• Korean Journal of Microbiology
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• v.32 no.2
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• pp.147-154
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• 1994

Nuclease was secreted to the environmental media from the Escherichia coli JM107 tranformant harboring the extracellular nuclease gene of Serratia marcescens in the plasmid of pNUC4. Under the growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth conditions, the amount of secreted enzyme was increased in parallel with bacterial growth. The enzyme was purified using chromatofraphic procedures of Matrex green gel and heparin agarose affinity gel, resulted in 50-fold purification with 15% recovery of the enzyme. The apparent molecular weight of the enzyme was estimated to be 29Kda by sodium dodecylsulfate denaturing gel electrophoresis. Using the purified enzyme, polyclonal antibody was obtained from the rabbit. The specificity of the antibody was confirmed by immunoblotting and immunoprecipitaion. For the investigation of cellular distribution of the enzyme, cells were fractionated into three fractions; cytoplasm, periplasm and extracellular fluid. While more than 80% of the enzymatic activity was detected in the extracellular fluid and periplasm, a little was found in the cytoplasm, indicating that the enzyme was likely to be immediately exported to the membrane for excretion after biosynthesis. These results were confirmed again by immunocytochemistry technique using the antibody.

• Information Systems Review
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• v.17 no.3
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• pp.77-94
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• 2015

As Internet has become a popular media for sharing information, users create and share tremendous volume of information including large amount of personal information in cyberspace. Sharing private information online can enhance strength of social relationship but it could also bring negative consequences like information privacy invasion. Although many companies and governments address the importance of information privacy online, there are countless cases of crimes and hackings relating personal information online world wide. Since there are some researches investigating the role of governments and organizations on online privacy domain but there is little research regarding users' privacy protection behaviors. This study investigates relationship between Internet users' information privacy protection behavior and environmental factors. Especially, this study focuses on users' behaviors regarding information privacy protection technology adoption. According to our research results, users' online privacy protective behaviors positively affected by governmental regulations expressed as an information privacy protection law. In addition, if user is allowed to use anonymity when he or she uses online services, they have more tendencies to adopt privacy protection technologies. The detailed research findings and contribution are discussed as well.

• Asia pacific journal of information systems
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• v.23 no.4
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• pp.29-51
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• 2013

In line with network technology development and smart device penetration, the social network service (SNS) has expanded its influence. The SNS which is a service based on communication and sharing among people, has grown based on users' voluntary engagement and participation and its influence has appeared beyond the cyberspace into the overall areas of domestic and foreign culture and society. In particular, SNS-based real-time communication during diverse disasters, can help prevent further damage. By sharing information on social donation activities and environmental campaigns, people have used SNS as a tool to change the society in a more positive way. Such series of activities functioning as a power to change the society have been made much faster and wider through the help of a new media called SNS. To better understand such trends, we are required to study about the SNS and its user relationships first. In this context, this study sought to identify the effects of people's desire to build friendships through SNS on the voluntary and society-friendly activities of people. This study considers online pro-social behavior and proposes online citizenship behavior. Citizenship behavior has been examined in organization context. That is, organizational citizenship behavior explains an employee's pro-social behavior in an organization context. Organizational citizenship behavior is characterized by the individual's helping others and promoting the functioning of the organization. By applying organizational citizenship behavior to an online context, we propose online citizenship behavior, an individual's pro-social behavior in an online context. An individual's pro-social behavior, i.e., online citizenship behavior, could be considered as a way for the better management of online community and society. It also needs to examine the development of online citizenship behavior. This study examined online citizenship behavior from the friendship desire. Because online society or community is characterized by online relationships between members, the friendship between members would lead to pro-social behavior, i.e., helping others and promoting the functioning of the online society, in such online context. This study further examines the antecedents of friendship desire in terms of SNS interactivity with its four factors. The findings based on the survey from real SNS users explain that the three factors of SNS interactivity (connectivity, enjoyment, and synchronicity) increases online friendship desire which then increases online citizenship behavior significantly. This study contributes to the literature by examining the key role of online friendship desire in leading to online citizenship behavior and identifying its antecedents in terms of SNS characteristics. The findings in this study also provide guidance on how to manage online society and how to promote the effective functioning of SNS.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.6 no.2
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• pp.209-221
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• 1996

The purpose of this study was to find a suitable cosolvent to $CS_2$ so that desorption efficiency can be improved for both polar and non-polar organic solvent mixtures collected on an activated charcoal tube. Cosolvents added to $CS_2$ include: DMF(N,N-dimethylformamide): $CS_2$ (v/v 1:99), DMF:$CS_2$(v/v 3:97), BC (butyl carbitol, 2-(2-butoxy ethoxy) ethanol):$CS_2$(v/v 1:99), and BC:$CS_2$(v/v 3:97)). The results obtained were as follows : 1. Comparing the desorption efficiency of $CS_2$ with those of $CS_2$ with 1, 3, 5 % DMF and 1, 3 % BC cosolvents for two different groups of charcoal tubes each containing 8 different polar and non-polar organic solvents with 3 different concentration levels, the desorption efficiencies of the cosolvent-added $CS_2$ increased significantly for all polar organic solvents regardless of concentration levels tested. For non-polar organic solvents, no noticeable improvement was detected except xylene and trichloroethylene. The desorption efficiency of xylene increased significantly while that of trichloroethylene increased significantly at the lowest concentration level tested. 2. Either 5 % DMF or 3 % BC was the most suitable cosolvent because the desorption efficiency for non-polar organic solvent mixtures was similar or slightly improved compared with that of $CS_2$, while those of for polar organic solvent mixtures were above 75 % except for cyclohexanone. 3. The smallest variations in desorption efficiency represented by the ratio calculated from the maximum to minimum desorption efficiency for all concentration levels tested were found when 3 % BC was used as a cosolvent. The above results indicate that the desorption efficiency of $CS_2$ particularly for polar organic solvent mixtures collected on a charcoal tube can be significantly improved by the use of cosolvents such as 5 % DMF or 3 % BC. A caution, however, is in order for selecting a cosolvent whenever the cosolvent itself is being used in the workplace or the impurities contained in the cosolvent may interfere with the analytical results. In addition, to improve desorption efficiencies for such organic solvents as cyclohexanone or ketones, it is recommended to use suitable collection and desorption media other than the traditional method of charcoal tube collection/$CS_2$ desorption.

• Journal of the Korea Safety Management & Science
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• v.22 no.2
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• pp.23-29
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• 2020

Korea's elevator industry is one of the world's eighth-largest industrial sectors and the third largest in the world by new installations. This year, the number of elevators has exceeded 700,000, and the number of new installations is 30,000-40,000 every year. However, the news of elevator-related accidents is reported continuously through the media and the accident rate is not decreasing. In particular, among the recent accidents related to elevators, accidents related to elevator workers are increasing, causing social problems. This year, the National Assembly's Environmental Labor Relations Commission's National Auditors lost five lives a year and 12 elevator workers were killed in fall and stenosis accidents during the installation, maintenance and replacement of the elevators for about two years since 2018. It took place to adopt the representatives of four domestic elevator companies as witnesses. An elevator worker is a collective term for workers involved in the design, manufacturing, installation, replacement, maintenance, inspection, management, and supervision related to the elevator industry, and the related accidents are called elevator worker accidents. Analysis of elevator-related accidents in the past has shown that the fault of the user accounted for 70% of the total, and the fault of the worker accounted for about 2.5%, and the accident occurred to the user or the user due to carelessness of the worker during the lift-related work. Currently, elevator-related accidents are reported by the Korea Elevator Safety Agency under Article 48 of the Elevator Safety Management Act under the Ministry of Interior and Safety. If deemed necessary for the prevention and prevention of recurrence of an elevator accident, the cause and condition of the elevator accident may be investigated. However, the current draft law is limited only to elevators after installation inspection, and is separated from the Ministry of Employment and Labor's data on accidents occurring in the manufacturing and installation stages related to the elevator industry. This study analyzes the recent safety accidents of elevator workers and prepares safety measures to prevent them through the risk analysis, and also draws out the problems and improvements of the current elevator worker accident investigation to find the elevator worker accident rate that is on the increase trend.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.5
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• pp.369-376
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• 2007

This experiment was carried out to investigate optimum conditions of coir-based substrates for the red pepper plug seedlings. Eleven different coir based substrates prepared by mixing of coir, vermiculite, rice hull, perlite, zeolite, mixed at different ratios were tested. The physical and chemical properties of the substrates were analyzed by the CEN (European committee for standardization) method. Fresh and dry weights of shoot and root, leaf area, root length, and T/R ratio (dry shoot weight/dry root weight) were determined at 55 days after sowing. The results showed that the growing media CRZ 8(coir:rice hull: vermiculite=8:1.9:0.1) and CVSZ 6(coir:silver vermiculite: zeolite=6:3.9:0.1) can successfully be used for pepper plug seedlings judging from dry weight and T/R ratio of the plug seedlings. The optimal range of total pore space, water volume, air volume, easily available water content and water buffering capacity of the coir-based growing substrates for pepper plug seedlings were in the range of 92~94%, 52~60%, 32~43%, 18~21%, and 0.9~8%, respectively.

• International Journal of Advanced Culture Technology
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• v.9 no.4
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• pp.18-28
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• 2021

This study surveyed how retirees' evaluation of starting food service business affects the effectiveness their new business and quality of life, based on personal factors such as entrepreneurship and business-starting capability and environmental factors by using questionnaires. Bootstrapping was carried out in order to find out factors affecting rapidly changing new business environments and retirees' initial intent to start a business so as to verify basic hypothesis about relation between retirees' evaluation of starting food service business (social, economic, and psychological effects) and the effectiveness of their new business and quality of life and confirm whether the effectiveness of the new business acts as a medium between the evaluation of starting food service business and quality of life. In addition, PLS-MGA was performed in order to verify whether the correlations among test factors can be varied according to the kind of job the target retirees had. Having examined the basic thesis, it was found that social and economic factors had significant positive effect on the effectiveness of the new business, and social and psychological factors had significant positive effect on the quality of life. Having analyzed whether the effectiveness of new business acted as a media between evaluation of starting food service business and quality of life, there was no significant effect as a medium. Having studied whether the kind of job of the retirees controlled or affected the relations among evaluation of starting food service business, effectiveness of new business and quality of life, the results were as follows: in the office job retiree group, the greater economic factor led to increase of effectiveness of new business, while social and psychological factors influenced the quality of life; In the physical labor group, the higher social factor resulted in higher effectiveness of new business, which showed significant positive effect on the quality of life. Having researched about which element is considered to be most important in starting food service business, the most important element was found to be dish/menu, followed by staff management, accounting management, business management, and service education. Having analyzed relation between accomplishment and important consideration for starting food service business, "managers with entrepreneurship," "appropriate distribution of time to prepare for starting business," and "operation of practical field education programs" showed higher importance compared to the degree of satisfaction, so it is needed to more concentrate on the above matters. This study intends to raise retirees' awareness of starting business and help them live better life based on the analysis results, and further suggest detailed mechanism and specific operations of factors affecting retirees' decision making on starting business, such that they can use the information as basic materials to make better choices that can lead to successful business.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.4
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• pp.326-332
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• 2021

Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.18 no.1
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• pp.74-82
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• 2019

The revolution of industry 4.0 is enabling us to build an intelligent connection society called smart cities. The use of renewable energy in particular solar energy is extremely important for modern society due to the growing power demand in smart cities, but its difficult to monitor and manage in each buildings since need to be deploy low energy sensors and information need to be transfer via wireless sensor network (WSN). The Internet of Things (IoT) / low-power wide-area (LPWA) is an emerging WSN technology, to collect and monitor data about environmental and physical electrical / electronics devices conditions in real time. However, providing power to IoT sensor end devices and other public electrical loads such as street lights, etc is an important challenging role because the sensor are usually battery powered and have a limited life time. In this paper, we proposes an efficient solar energy-based power management scheme for smart city based on IoT technology using LoRa wide-area network (LoRaWAN). This approach facilitates to maintain and prevent errors of solar panel based energy systems. The proposed solution maximizing output the power generated from solar panels system to distribute the power to the load and the grid. In this paper, we proved the efficiency of the proposed system with Simulink based system modeling and real-time emulation.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.197-202
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• 2019

In the era of the fourth revolution, interest in content production using proven engines in the broadcasting sector, such as Vizrt, is growing. The new visual effects required in the 5G era are critical to content production training. Vizrt has a good production time utility and affordability for broadcast and media content. In this paper, we are going to use this to present a practical case of the theorem and application of the basic training course in the production of virtual content, and to present the basic training direction. In the introduction, the graphic algorithm analyzed and studied the characteristics and environmental factors of the Vizrt engine. In this paper, the production process was studied separately, and the work carried out through engine implementation was presented. The VS Studio Foundation was provided as a practical production case at each stage. The Vizrt engine operator process is important in graphic approach and application, and through the results of the lecture, the method of understanding and implementing algorithms for virtual reality perspective suitable for basic learning was studied. Based on practice, the research method of main theory was to create Vizrt contents specialized in 5G contents work in each sector and to implement graphic production in new areas from contents image. Through this study, we came to the conclusion of the basic training method through virtual reality content work based on Vizrt by practicing content creation according to the subject. It also proposes the effect of creating Vizrt content and the direction of building Vizrt basic training courses.