• Title/Summary/Keyword: Environmental forensic

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Embryotoxic and Teratogenic Effects of Tartrazine in Rats

  • Hashem, Mohamed Mohammed;Abd-Elhakim, Yasmina Mohammed;Abo-EL-Sooud, Khaled;Eleiwa, Mona M.E.
    • Toxicological Research
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    • v.35 no.1
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    • pp.75-81
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    • 2019
  • Tartrazine (TAZ) is one of the most commonly used artificial dyes for foods and drugs. We determined the effect of TAZ on fetal development by examining morphological, visceral, and skeletal malformations in rat fetuses following daily oral administration of TAZ to pregnant Wistar rats at the 6th-15th day of gestation. TAZ at 0.45 and 4.5 mg/kg induced 6.0 and 7.1% fetal resorptions, as well as 10.0 and 10.5% fetal mortality, respectively. Fetal body weight and length were significantly lower in the groups treated with TAZ at 0.45 ($3.97{\pm}0.21g$ and $27.3{\pm}0.54mm$, respectively) and 4.5 mg/kg ($3.48{\pm}0.15g$ and $23.22{\pm}1.02mm$, respectively) than in the control group ($4.0{\pm}0.15g$ and $30.01{\pm}0.42mm$, respectively). TAZ at 0.45 and 4.5 mg/kg induced hepatic damage (20 and 33.3%, respectively), dark brown pigmentation due to hemosiderin in the splenic parenchyma (16.7 and 21.7%, respectively), as well as destructed and necrotic renal tubules (16.7 and 26.7%, respectively) in the fetuses. Moreover, TAZ at 0.45 and 4.5 mg/kg caused one or more missing coccygeal vertebrae (20 and 40%, respectively), missing sternebrae (6 and 10%, respectively), missing hind limbs (24 and 4%, respectively), and irregular ribs (16 and 20, respectively) in the fetuses. We concluded that TAZ has embryotoxic and teratogenic potentials in rats.

Identification of Jet fuel (JP-8) in Petroleum Hydrocarbon Contaminated Soil through the Qualitative Analysis of Antioxidants (유류 오염 토양 중 산화방지제 정성 분석을 통한 항공유(JP-8) 유종 판별)

  • Kim, Yonghun;Lee, Goontaek;Jang, Hanjeon;Jo, Yunju;Kim, Moongun;Choi, Jaeho;Kang, Jiyoung
    • Journal of Soil and Groundwater Environment
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    • v.27 no.4
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    • pp.37-48
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    • 2022
  • Accurate analysis of petroleum hydrocarbons in soil is an important prerequisite for proper source tracking of contamination. Identification of petroleum compounds is commonly carried out by peak pattern matching in gas chromatography. However, this method has several technical limitations, especially when the soils underwent biological, physical and chemical transformation. For instance, it is very difficult to distinguish jet fuel (JP-8) from kerosene because JP-8 is derivatized from secondary reaction between chemical agents (e.g. anti-oxidants, antifreezer and so on) and kerosene. In this study, an alternative method to separately analyze JP-8 and kerosene in the petroleum hydrocarbon contaminated soil was proposed. Qualitative analyses were performed for representative phenolic antioxidants [2,6-di-tert-butyl phenol (2,6-DTBP), 2,4-di-tert- butylphenol(2,4-DTBP), 2,6-di-tert-butyl-4-methyl phenol (2,6-DTBMP)] using a two dimensional gas chromatograph mass spectrometer (2D GC×GC-TOF-MS). This qualitative analysis of antioxidants in soil would be a useful complementary tool for the peak pattern matching method to identify JP-8 contamination in soil.

Multi-Behavior Analysis Based on Google Archiving Data (구글 아카이빙 데이터 기반 멀티 행위 분석)

  • Yeeun Kim;Sara Hong;Seongmin Kim
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.33 no.5
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    • pp.737-751
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    • 2023
  • The importance of digital forensics in the cloud environment is increasing as businesses and individuals move their data from On-premise to the cloud. Cloud data can be stored on various devices, including mobile devices and desktops, and encompasses a variety of user behavior artifacts, such as information generated from linked accounts and cloud services. However, there are limitations in securing and analyzing digital evidence due to environmental constraints of the cloud, such as distributed storage of data and lack of artifact linkage. One solution to address this is archiving services, and Google's Takeout is prime example. In this paper, user behavior data is analyzed for cloud forensics based on archiving data and necessary items are selected from an investigation perspective. Additionally, we propose the process of analyzing selectively collected data based on time information and utilizing web-based visualization to meaningfully assess artifact associations and multi-behaviors. Through this, we aim to demonstrate the value of utilizing archiving data in response to the increasing significance of evidence collection for cloud data.

Multidimensional data generation of water distribution systems using adversarially trained autoencoder (적대적 학습 기반 오토인코더(ATAE)를 이용한 다차원 상수도관망 데이터 생성)

  • Kim, Sehyeong;Jun, Sanghoon;Jung, Donghwi
    • Journal of Korea Water Resources Association
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    • v.56 no.7
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    • pp.439-449
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    • 2023
  • Recent advancements in data measuring technology have facilitated the installation of various sensors, such as pressure meters and flow meters, to effectively assess the real-time conditions of water distribution systems (WDSs). However, as cities expand extensively, the factors that impact the reliability of measurements have become increasingly diverse. In particular, demand data, one of the most significant hydraulic variable in WDS, is challenging to be measured directly and is prone to missing values, making the development of accurate data generation models more important. Therefore, this paper proposes an adversarially trained autoencoder (ATAE) model based on generative deep learning techniques to accurately estimate demand data in WDSs. The proposed model utilizes two neural networks: a generative network and a discriminative network. The generative network generates demand data using the information provided from the measured pressure data, while the discriminative network evaluates the generated demand outputs and provides feedback to the generator to learn the distinctive features of the data. To validate its performance, the ATAE model is applied to a real distribution system in Austin, Texas, USA. The study analyzes the impact of data uncertainty by calculating the accuracy of ATAE's prediction results for varying levels of uncertainty in the demand and the pressure time series data. Additionally, the model's performance is evaluated by comparing the results for different data collection periods (low, average, and high demand hours) to assess its ability to generate demand data based on water consumption levels.

Effects of the Temperature and Light Intensity on the Growth and Microcystin Production of Three Species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. viridis) (Microcystis 3종(M. aeruginosa, M. ichthyoblabe, M. viridis)의 성장과 microcystins 생성에 대한 온도 및 조도의 영향)

  • Lee, Kyung-Lak;Jheong, Weon-Hwa;Kim, Jin-Hee;Kim, Han-Soon
    • Korean Journal of Ecology and Environment
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    • v.43 no.3
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    • pp.400-408
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    • 2010
  • The growth and microcystins production characteristics of three species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. viridis) isolated from Yeongchun dam and Ankei dam in Kyungpook Province, South Korea were investigated at temperatures of $15{\sim}35^{\circ}C$ and light intensities of $35{\sim}180\;{\mu}mol\;m^{-2}\;s^{-1}$. All of the three species exhibited the highest growth rates (${\mu}_{max}$) over the $30^{\circ}C$. The maximum growth rates of M. aeruginosa and M. ichthyoblabe was observed at $70\;{\mu}mol\;m^{-2}\;s^{-1}$, while M. viridis showed maximum growth rate at $35\;{\mu}mol\;m^{-2}\;s^{-1}$. The maximum production of total microcystins was observed at $20^{\circ}C$, and the production of microcystins decreased according as temperature increase. The highest microcystins production of M. aeruginosa, M. ichthyoblabe and M. viridis observed at light intensities of $120\;{\mu}mol\;m^{-2}\;s^{-1}$, $70\;{\mu}mol\;m^{-2}\;s^{-1}$ and $35\;{\mu}mol\;m^{-2}\;s^{-1}$, respectively. The concentration of microcyst in production and microcystin types of three species according to temperatures and light intensities showed clear difference between the species.

Comparison of Optimal Storage Temperature and Collection Reagents for Living Bacterial Cells in Swab Samples (면봉시료에서 세균의 보존을 위한 최적 보관 온도와 채취 시약의 비교)

  • Lee, Yeong Ju;You, Hee Sang;Lee, Song Hee;Lee, So Lip;Lee, Han;Sung, Ho Joong;Kang, Hee Gyoo;Hyun, Sung Hee
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.4
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    • pp.326-332
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    • 2021
  • Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

Trace element analysis of korean car windshield using LA-ICP-MS (LA-ICP-MS를 이용한 한국 자동차 유리의 미량원소 분석)

  • Min, Ji-Sook;Choi, Man-Sik;Heo, Sang-Cheol;Kim, Jae-Kyun
    • Analytical Science and Technology
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    • v.22 no.3
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    • pp.235-246
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    • 2009
  • The analyses of minor and trace elements in glass debris were performed using LA-ICP-MS in order to identify manufacturers using real commercial samples. At first, a calibration curve was made using standard glass samples of NIST 610, 612, 614 and 616. $^{29}Si$ was used as an internal standard, and the ratios of metal/Si for each metal were compared with their concentrations. Based on elements in each sample and standard materials, 24 metals were quantified and the LOD in analysis, according to the blank sample, was in the range of 0.11 mg/kg (Ti)-4.91 mg/kg (Ca). Eleven samples from two manufacturers were collected and five sub-samples were taken from each sample for analysis. 15 elements (Co, Ce, Ca, Mn, Sr, Ba, Li, Rb, U, La, Th, Na, Al, Zr and Hf) were selected to identify manufacturers because some elements (Cu, Cr, Cd and Ni) were below the detection limit and some elements (Ti, Pr, Mg, Nb, Nd) were absent in the analysis of standards and others (Pb and Sn) had a problem of homogeneity. The attempts to identify manufacturers and the manufacturing period were performed through a triangular diagram. In the manufacturer discrimination by discriminant analysis, a canonical discriminant function was made based on Mn, Ce and Rb, and each sample could be identified.