• Title/Summary/Keyword: Environmental flow

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A novel method for cell counting of Microcystis colonies in water resources using a digital imaging flow cytometer and microscope

  • Park, Jungsu;Kim, Yongje;Kim, Minjae;Lee, Woo Hyoung
    • Environmental Engineering Research
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    • v.24 no.3
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    • pp.397-403
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    • 2019
  • Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.

MATHEMATICAL MODEL OF SULFUR UTILIZING AUTOTROPHIC DENITRIFICATION IN AN UP-FLOW PACKED-BED REACTOR BASED ON BIOMASS DISTRIBUTION

  • Park, Woo-Shin;Ahn, Yoeng-Hee;Jung, Kyung-Ja;Tatavarty, Rameshwar;Kim, In-S.
    • Environmental Engineering Research
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    • v.10 no.4
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    • pp.191-198
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    • 2005
  • A novel technology for the removal of nitrogen from wastewater, an autotrophic denitrification process with sulfur particles, has been developed. A respirometer was employed to monitor the nitrogen gas produced in the reactor, while 4',6-diamidino-2-phenylindole staining was employed to investigate the biomass distribution in terms of cell number according to the reactor height. From the respirometric monitoring, the denitrification reaction was defined as a first order reaction. The reactor was divided into 7 sections and biomass was analyzed in each section where cell number was ranged from $4.8\;{\times}\;10^6\;to\;8.7\;{\times}\;10^7$ cells/g dry weight of sulfur. Cells placed mostly in the lower layer ( < 10 cm of height). A function for biomass distribution was obtained with non-linear regression. Then a mathematical model has been developed by combining a plug-flow model with the biomass distribution function. The model could make a vertical profile of the up-flow packed-bed reactor resulting in a reasonable comparison with measured nitrate concentration with 5% of error range.

Design Verification of Environmental Control System by Flow Balance Test (유량평형시험을 통한 환경제어계통 설계 검증)

  • Park, Dong-Myung;Joung, Yong-In;Moon, Woo-Yong;Park, Sung-Sun
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.40 no.7
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    • pp.608-615
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    • 2012
  • In this study, we analyzed the system impedance of Unmanned Aerial Vehicle avionics bay and Environmental Control System(ECS), and estimated the proper air flow rate to be supplied avionics equipments. As the result of estimation, we evaluated the performance of ECS after analysing the flow balance rate and the air flow rate about each outlet port, and simultaneously decided the flow balance rate after evaluating the thermal substantiality by the thermal analysis of avionics bay. In order to verify the property of analysis result, we conducted the flow balance test using the actual avionics equipments and finally deduced the flow rate to be met system requirements of avionics equipments. Also, as the analysis results, we verified the satisfaction of system requirements at midium altitude condition and proved the performance characteristics of an Environmental Control System(ECS).

A Economic Feasibility Study on Environmental Ecology Flow Supply Plan using Agricultural Reservoir - Focused on Dongbok River - (농업용저수지를 이용한 환경생태유량 확보방안 경제성 비교 연구 - 동복천을 중심으로 -)

  • Park, Jin-hyeon;Ko, Jae han;Sung, Mu-hong;Jung, Hyoung-mo;Park, Tae-sun;Kwak, Yeong Cheol;Choi, Woo-young;Boem, Jina;Jeung, Minhyuk;Yoo, Seung-hwan;Yoon, Kwang-sik
    • Journal of The Korean Society of Agricultural Engineers
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    • v.61 no.5
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    • pp.33-47
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    • 2019
  • The environmental ecological flow (EEF) of stream or river will be designated according to the enforcement of the Water Environment Preservation Act. Previous researches by Ministry of Environment have proposed agricultural reservoirs as alternative resources to secure river flow where multi-purpose dam does not exist. However, agricultural reservoirs are constructed for the supply of agricultural water, and in fact, there is not a sufficient amount of water to be supplied to rivers as EEF. Therefore, this study examines the economic feasibility of securing EEF through the remodeling or construction of agricultural reservoirs. We investigated water balance of reservoir through simulation of three types of water supply demands such as agricultural water, agricultural water and river maintenance flow, and agricultural water and environmental ecology flow. The economics analysis was conducted on water supply demands and corresponding remodeling or construction of reservoirs. As a result, it was found that the method of securing through heightening existing reservoir enhancement is economically feasible. However, it was not possible to secure all the amount of the EEF due to the size limitation of existing reservoirs or constrain of the watershed for newly built reservoir. Therefore, in order to secure all of the EEF, the utilization of other alternatives as well as agricultural reservoirs should be considered. This study demonstrated the method of economical feasibility study of securing river maintenace flow and EEF using agricultural reservoirs and other considerations.

Determination of Flow Patterns for Multi-Phase Flow in Petroleum Production Systems (석유생산 시스템에서 다상유동의 패턴 결정)

  • Lee, Kun-Sang;Kim, Hyun-Tae
    • Economic and Environmental Geology
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    • v.44 no.2
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    • pp.153-159
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    • 2011
  • A comprehensive mechanistic model has been used to determine the flow pattern for gas-oil two-phase flow in pipes of petroleum production system. Depending on operational parameters, geometrical variables, and physical properties of the two phases, the two phases shows a specific flow patterns. For different parameters of the system, How pattern were compared for wide range of superficial velocities of oil and gas. In a variety of parameters, the inclinational angle and superficial velocities of oil and gas are the most dominant factors in determining the flow patterns for two-phase flow in pipelines. Other parameters such as pipe diameter and fluid properties have a limited effect on the change of flow patterns except for near transition. The mechanistic model is shown to be useful to determine the flow pattern in situations where either an experimental evaluation in a laboratory or reliable correlations are not available.

Current Status of Refractory Dissolved Organic Carbon in the Nakdong River Basin (낙동강유역 난분해성 용존 유기탄소 배출 현황 분석)

  • Lee, Jeonghoon;Kim, Jungsun;Lee, Jae Kwan;Kang, Limseok;Kim, Sangdan
    • Journal of Korean Society on Water Environment
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    • v.28 no.4
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    • pp.538-550
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    • 2012
  • This study suggests a general methodology which is designed for assessing RDOC behavior at the catchment scale by coupling properly a series of steam flow and water quality simulation models and actual monitoring data set. The modified TANK model in which a river routing function is incorporated to the conventional one is applied to simulate the long-term daily stream flow data, and the simulated stream flow data is combined with the 7-parameter log-linear model coupled to the minimum variance unbiased estimator to simulate the long-term daily water quality (BOD, COD and TOC) loads. Finally, the regression analysis between the usually monitored water quality data (BOD, COD and TOC) and RDOC is combined with the simulated water quality data to manifest the spatio-temporal variability of RDOC flux behavior at the Korean TMDL catchment scale.

Reuse Possibility of By-pass Flow and Secondary Effluent using BAF (BAF를 이용한 2차 처리수의 재이용 가능성 및 강우시 하수처리장 월류수의 처리)

  • An, J.H.;Park, J.B.;Kim, S.W.;Park, J.H.;Ha, J.S.;Choi, E.
    • Journal of Korean Society on Water Environment
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    • v.21 no.2
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    • pp.190-195
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    • 2005
  • The laboratory and pilot scale BAFs (biological aerated filters) were operated with 0.3 hr to 1.1 hr EBCT(empty bed contact time) at a maximum filtration rate of $472m^3/m^2/day$ as a treatment method for reuse of secondary effluent and by-pass flow in this study. The effluent BOD and SS were generally 3.5 to 5 mg/L and 2 to 3 mg/L, respectively with 2ndary effluent, but the SS concentrations increased to 4 to 8 mg/L with the increased flow rates of by-pass flow. Potential nitrification rates were very high, but the nitrogen removal efficiencies were low due to the limited carbon sources. Bypass of a part of primary effluent seemed to be desirable to increase the nitrogen removal. Disinfection must be furnished for the reuse of BAF effluent.

Ammonia Nitrogen Removal in Wastewater Using Microwave Irradiation (마이크로웨이브를 이용한 폐수 내 고농도 암모니아성질소 제거)

  • Shin, Soyeun;Koo, Bonheung;Kim, Taehyun;Lee, Yuhak;Ahn, Johng-Hwa
    • Journal of Korean Society on Water Environment
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    • v.30 no.5
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    • pp.486-490
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    • 2014
  • Industrial use of microwave heating as an alternative to conventional heating is becoming popular mainly due to dramatic reductions in reaction time. Therefore, this work experimentally determined the effect of microwave irradiation on ammonia nitrogen removal in wastewater. The effects of air flow rate (0.3~0.9 L/min), treatment temperature ($70{\sim}100^{\circ}C$), and initial pH (9~11) were characterized. As the air flow rate increased from 0.3 to 0.9 L/min, the ammonia removal rate constant (k) increased from -0.6642 to $-1.0755min^{-1}$. As the temperature increased from 70 to $100^{\circ}C$, k increased -0.0338 to $-1.0755min^{-1}$. As the pH increased from 9 to 11, k increased -0.2443 to $-1.0755min^{-1}$. Ammonia removal was strongly dependent on temperature and pH rather than air flow rate. The results show that microwave irradiation is effective in ammonia nitrogen removal in wastewater due to advantages of fast and effective processing.

Study on the Management of Minimum Low Flow and Water Quality of Hongjechun (홍제천 정비를 위한 하천유지유량 확보 및 수질개선 방안 연구)

  • Hwang, Byung-Gi;Ho, Jong-Kwang;Oh, Kyung-Seok
    • Journal of Environmental Impact Assessment
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    • v.14 no.1
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    • pp.37-46
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    • 2005
  • In this study, field surveys were performed for 12 stations in main stream of Hongjechun and 3 stations in 2 tributaries, respectively, in order to find out dried reaches of the stream, and to examine the water quality of the stream, and to suggest methods to improve the stream concerned into eco-stream. In the results of water quality in stream, however distinct difference for seasonal variation of the water quality was not found, the water quality of winter was relatively better than that of other seasons. Annual mean concentration of BOD was 6.5mg/L in the upper reach, 11.8mg/L in the middle reach, 15.3mg/L in the lower reach of main stream, and total mean was 12.5mg/L, while the BOD concentration was 3.6mg/L in the upper reach, and was 9.6mg/L in the low reach of Gukichun, the tributary. Based on flow examination, the level of water depth was so low and the flow can not be traveled downstream in the reach between ST-9 and ST-10 for low water season, whereas it was observed that the flow was traveling except the dry season even the water level was lower than that of adjacent stations.

Transformation of dissolved organic matter in a constructed wetland: A molecular-level composition analysis using pyrolysis-gas chromatography mass spectrometry

  • Park, Jongkwan;Choi, Mijin;Cho, Jaeweon;Chon, Kyongmi
    • Environmental Engineering Research
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    • v.23 no.4
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    • pp.390-396
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    • 2018
  • This study investigated the transformation of dissolved organic matter (DOM) in a free-water surface flow constructed wetland. Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) coupled with preparative high-performance liquid chromatography (prep-HPLC) was used to analyze the compositions of biopolymers (polysaccharides, amino sugars, proteins, polyhydroxy aromatics, lipids and lignin) in DOM according to the molecular size at three sampling points of the water flow: inflow, midflow, and outflow. The prep-HPLC results verified the decomposition of DOM through the decrease in the number of peaks from three to one in the chromatograms of the sampling points. The Py-GC/MS results for the degradable peaks indicated that biopolymers relating to polysaccharides and proteins gradually biodegraded with the water flow. On the other hand, the recalcitrant organic fraction (the remaining peak) in the outflow showed a relatively high concentration of aromatic compounds. Therefore, the ecological processes in the constructed wetland caused DOM to become more aromatic and homogeneous. This indicated that the constructed wetland can be an effective buffer area for releasing biochemically stable DOM, which has less influence on biological water quality indicators, e.g., biochemical oxygen demand, into an aquatic ecosystem.