• Title/Summary/Keyword: Environmental Tests

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Risk Assessment of Pesticide for Earthworms (농약의 지렁이에 대한 위해성 평가)

  • Park, Kyung-Hun;Park, Yeon-Ki;Joo, Jin-Bok;Kyung, Kee-Sung;Shin, Jin-Sup;Kim, Chan-Sub;Park, Byung-Jun;Uhm, Jae-Youl
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.280-287
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    • 2003
  • To assess the risk of pesticides on earthworm, the acute toxicities of 10 pesticides were investigated and their toxicity exposure ratios(TERs) were calculated. As the TERs of paraquat dichloride and pendimethalin were more than 100, their risks were rated negligible. Risk of benfuracarb, cadusafos, chlorpyrifos-methyl, endosulfan, isazofos and parathion which have TERs of $10\sim100$ were rated low. However, risk of imidacloprid and phorate which have TER of less than 10 were estimated highly to need a reproduction study. Earthworms were exposed to twenty two pesticides including dazomet 98% GR having PECs of more than $5mg{\cdot}kg^{-1}$ in artificial soil at standard and double dose for 14 days. All the earthworms exposed to dazomet 98% GR and metam-sodium 25% SL were died to show their high risk, while no serious adverse effects were observed in the soil treated with 15 pesticides, calcite 95% WP, calcium polysulfide 36% CF, chlorothalonil 75% WP, daminozide 85% WP, dichlonil 6.7% GR, etridiazole 25% EC, fosetyl-Al 80% WP, glyphosate 41 % SL, hymexazol 30% SL, iprodione 50% WP, machine oil 95% EC, mancozeb 75% WP, propineb 70% WP, terbuthylazine 80% WP and triazophos 40% EC. In case of thiophanate-methyl 70% WP, copper hydroxide 77% WP, dimethoate 46% EC, tolclofos-methyl 50% WP and propamocarb hydrochloride 67% SL, any effect did not show clearly, suggesting an additional subchronic toxicity study. The risk of thiophanate-methyl 70% WP to earthworm was estimated high, considering its subchronic effect, while effects of copper hydroxide 77% WP, dimethoate 46% EC, tolclofos-methyl 50% WP and propamocarb hydrochloride 67% SL to earthworms were negligible, considering no adverse effects in subchronic tests.

The Synthetic Study of Environmental Contamination at the Seokdae Municipal Waste Landfill in Pusan (부산 석대 생활폐기물 매립장의 환경오염에 대한 종합적 연구)

  • 김병우;정상용;이민희;이병헌
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.04a
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    • pp.98-103
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    • 2001
  • In order to understand the characteristics of leachate at the Seokdae municipal waste landfill in the Pusan city, the correlation between leachate pollution loading and volume of gas production. concentration of gas and subsidence of ground, the characteristical methos, geochemical analyses and laboratory column tests using samples of gases, leachate and surface soil of Seokdae waste landfill area. Through the analysis of water balance, leachate flow rate and pollution loading were estimated. Geistatistical analysis of four gas components ( $O_2$, C $H_4$, $H_2$S and CO) shows the possibility of ground subsidence around the group of a site with high concentration of gas. From geochemical analyses of leachate, EC and Total-Alkalinity of ground subsidence around the group of a site with high concentration of gas. From geochemical analysis of leachate, Ec and Total-Alkalinity were increased, and Cl, Cr, Mn, Cu, Zn, Cd and Pb were decreassed comparing to the part, and the type of water quality was Na-HC $O_3$ in trilinear diagram. It shows that biodecomposition of municipal wastes continues actively. From the analysis of water balance, the total leachate flow rate is about 465.11㎥/day and pure pollution loading of Cl, Mn and Fe are estimated to 223.8kg/day, 0.2kg/day, 0.3kg/day, respectively. The laboratory column test of residual soil and landfill soil shows 0.206cm and 0.019cm for linear velocity(equation omitted), 0.234 $\textrm{cm}^2$/min and 0.018$\textrm{cm}^2$/min for diffusion coefficient ( $D_{ι}$), and 1.136cm and 0.095cm longitudinal dispersion index ($\alpha$$_{ι}$), respective]y. It demonstrates that the delay time of contamination for residual soil is shorter than that of landfill soil.

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Microstructural Study of Mortar Bar on Akali-Silica Reaction by Means of SEM and EPMA Analysis (알칼리-실리카 반응에 의한 모르타르 봉의 SEM과 EPMA 분석을 통한 미세구조 연구)

  • Jun, Ssang-Sun;Lee, Hyo-Min;Jin, Chi-Sub
    • Journal of the Korea Concrete Institute
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    • v.21 no.4
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    • pp.531-537
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    • 2009
  • In this study alkali reactivity of crushed stone was conducted according to the ASTM C 227 that is traditional mortar bar test, and C 1260 that is accelerated mortar bar test method. The morphology and chemical composition of products formed in mortar bar, 3 years after the mortar bar tests had been performed, were examined using scanning electron microscopy (SEM) with secondary electron imaging (SEI) and electron probe microanalysis (EPMA) with backscattered electron imaging (BSEI). The crushed stone used in this study was not identified as being reactive by ASTM C 227. However, mortar bars exceeded the limit for deleterious expansion in accelerated mortar bar test used KOH solution. The result of SEM (SEI) analysis, after the ASTM C 227 mortar bar test, confirmed that there were no reactive products and evidence of reaction between aggregate particles and cement paste. However, mortar bars exposed to alkali solution (KOH) indicated that crystallized products having rosette morphology were observed in the interior wall of pores. EPMA results of mortar bar by ASTM C 227 indicated that white dots were observed on the surface of particles and these products were identified as Al-ASR gels. It can be considered that the mortar bar by ASTM C 227 started to appear sign of alkali-silica reaction in normal condition. EPMA results of the mortar bar by ASTM C 1260 showed the gel accumulated in the pores and diffused in to the cement matrix through cracks, and gel in the pores were found to be richer in calcium compared to gel in cracks within aggregate particles. In this experimental study, damages to mortar bars due to alkali-silica reaction (ASR) were observed. Due to the increasing needs of crushed stones, it is considered that specifications and guidelines to prevent ASR in new concrete should be developed.

Dynamic Response of Tension Leg Platform (Tension Leg Platform의 동적응답에 관한 연구)

  • Yeo, Woon Kwang;Pyun, Chong Kun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.5 no.1
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    • pp.21-30
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    • 1985
  • The tension leg platform (TLP) is a kind of compliant structures, and is also a type of moored stable platform with a buoyancy exceeding the weight because of having tensioned vertical anchor cables. In this paper, among the various kinds of tension leg structures, Deep Oil Technology (DOT) TLP was analyzed because it has large-displacement portions of the immersed surface such as vertical corner pontoons and small-diameter elongated members such as cross-bracing. It also has results of hydraulic model tests, comparable with theorectical analysis. Because of the vertical axes of symmetry in the three vertical buoyant legs and because there are no larger horizontal buoyant members between these three vertical members, it was decided to develop a numerical algorithm which would predict the dynamic response of the DOT TLP using the previously developed numerical algorithm Floating Vessel Response Simulation (FVRS) for vertically axisymmetric bodies of revolution. In addition, a linearized hydroelastic Morison equation subroutine would be developed to account for the hydrodynamic pressure forces on the small member cross bracing. Interaction between the large buoyant members or small member cross bracings is considered to be negligible and is not included in the analysis. The dynamic response of the DOT TLP in the surge mode is compared with the results of the TLP algorithm for various combinations of diffraction and Morison forces and moments. The results which include the Morison equation are better than the results for diffraction only. This is because the vertically axisymmetric buoyant members are only marginally large enough to consider diffractions effects. The prototype TLP results are expected to be more inertially dominated.

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A Study on the Fatigue Fracture Behavior in Butt Welded Joints of Steel Structures (강구조물(鋼構造物) 맞대기 용접연결부(鎔接連結部)의 피로파괴거동(疲勞破壞擧動)에 관한 연구(硏究))

  • Park, Je Seon;Chung, Yeong Wha;Kim, Jeong Tae
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.6 no.3
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    • pp.53-62
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    • 1986
  • For the research on the fatigue fracture behavior in the welded joints of steel structures, base metal specimens and welded ones were selected, and the direct fatigue tests were carried out. Thereafter, fatigue-life (S-N) curves, plastic strain-number of cycles (${\varepsilon}_p$-N) curve, the extrapolated fatigue-life (${\varepsilon}_p$-$N_c$) curve, and da/dN-${\Delta}K$ curves were plotted. By these results the followings were obtained. It was shown that the ratio of fatigue strength at $2{\times}10^6$ cycles of the welded specimen to that of the base metal one was 0.6, and that 0.72 for the base metal and 0.65 for the welded one were the ratio of fatigue strength at $2{\times}10^6$ cycles to yielding stress. The S-N curve for the welded specimen was separated into two sections, the low gradient section and the steep section. As this result, it was shown that the more stress became to reduce, the more the reduction of fatigue strength became to be great. It was shown that fatigue strength at $2{\times}10^6$ cycles from this case was about 83 % of that from the S-N curve plotted with one section. It was thought that the reason was that weld flaw acted greatly on the fatigue strength within the low stress range. It was shown that at the instart of crack initiation plastic strain increased abrupt1y in the case of the welded specimen more than the case of the base metal specimen, and increased abruptly in the upper stress range in both cases. It was shown that the experimental constant ${\alpha}$, 0.42, in the base metal nearly accorded with Manson-Coffin's result, but this made a great difference with the case in the welded specimen. It was thought that it was due to the abrupt change of plastic strain and the influence of weld flaw.

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The Optimal Configuration of Arch Structures Using Force Approximate Method (부재력(部材力) 근사해법(近似解法)을 이용(利用)한 아치구조물(構造物)의 형상최적화(形狀最適化)에 관한 연구(研究))

  • Lee, Gyu Won;Ro, Min Lae
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.2
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    • pp.95-109
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    • 1993
  • In this study, the optimal configuration of arch structure has been tested by a decomposition technique. The object of this study is to provide the method of optimizing the shapes of both two hinged and fixed arches. The problem of optimal configuration of arch structures includes the interaction formulas, the working stress, and the buckling stress constraints on the assumption that arch ribs can be approximated by a finite number of straight members. On the first level, buckling loads are calculated from the relation of the stiffness matrix and the geometric stiffness matrix by using Rayleigh-Ritz method, and the number of the structural analyses can be decreased by approximating member forces through sensitivity analysis using the design space approach. The objective function is formulated as the total weight of the structures, and the constraints are derived by including the working stress, the buckling stress, and the side limit. On the second level, the nodal point coordinates of the arch structures are used as design variables and the objective function has been taken as the weight function. By treating the nodal point coordinates as design variable, the problem of optimization can be reduced to unconstrained optimal design problem which is easy to solve. Numerical comparisons with results which are obtained from numerical tests for several arch structures with various shapes and constraints show that convergence rate is very fast regardless of constraint types and configuration of arch structures. And the optimal configuration or the arch structures obtained in this study is almost the identical one from other results. The total weight could be decreased by 17.7%-91.7% when an optimal configuration is accomplished.

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Analysis of Hemolytic Microflora from the Ark Shell (Scapharca broughtonii) (패류(Scapharca broughtonii) 유래의 용혈활성 미생물 다양성 분석)

  • Kim, Dong-Gyun;Nam, Bo-Hye;Kong, Hee-Jeong;Kim, Woo-Jin;Kim, Bong-Seok;Jee, Young-Ju;Lee, Sang-Jun;Jung, Choon-Goo;Kong, Mi-Sun;Kim, Young-Ok
    • Journal of Life Science
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    • v.22 no.5
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    • pp.642-649
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    • 2012
  • The southern coast of Korea is important for the ark shell ($Scapharca$ $broughtonii$) aquaculture, but the productivity was rapidly reduced during the previous decade by mass mortality. To overcome this economic loss, investigations only focused on environmental factors, and microbiological researches were performed insufficiently. In this study, two sites (Gangjin and Jinhae bay) were selected for their high and low rate of mortality, respectively, and the existence of microflora from underwater sediments in the bodies of $S.$ $broughtonii$ was analyzed. We screened the whole body of each sample and chose unique colonies, which exhibit alpha- and beta-hemolytic activity, for identification. The microflora in $S.$ $broughtonii$ was less variable than sediments, and restricted species were isolated. We identified 17 genera of 88 species and 16 genera of 64 species from the two bays, respectively. A major proportion was comprised of $Bacillus$ species, with the $Bacillus$ $cereus$ group being the most common species among the $Bacillus$ strains, while $Paenibacillus$, $Lynsilbacillus$, and $Vibrio$ species were the second most abundant species. At the genus level, there were no significant microbial differences between the two coastal regions. 64 species were isolated from rare site (Jinhae bay), but more species (88) with greater variety were isolated from the frequent site (Gangjin bay). Therefore, it was assumed that the cause of mass mortality lay in the difference in specie-level diversity, and conducting investigations on the diagnosis of pathogenic species by challenging tests using isolated unique species.

Diagnostic Performance of HPV E6/E7 mRNA and HPV DNA Assays for the Detection and Screening of Oncogenic Human Papillomavirus Infection among Woman with Cervical Lesions in China

  • Wang, Hye-young;Lee, Dongsup;Park, Sunyoung;Kim, Geehyuk;Kim, Sunghyun;Han, Lin;Yubo, Ren;Li, Yingxue;Park, Kwang Hwa;Lee, Hyeyoung
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7633-7640
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    • 2015
  • Background: Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it is responsible for most cases of cervical uterine cancer. Although HPV infections of the cervix do not always progress to cancer, 90% of cervical cancer cases have been found to be associated with high risk HPV (HR-HPV) infection. HPV DNA testing is widely used, along with Papanicolaou (Pap) testing, to screen for cervical abnormalities. However, there are no data on the prevalence of genotype-specific HPV infections assessed by measuring HPV E6/E7 mRNA in women representative of the Chinese population across a broad age range. Materials and Methods: In the present study, we compared the results with the CervicGen HPV RT-qDx assay, which detects 16 HR-HPV genotypes (Alpha-9: HPV 16, 31, 33, 35, 52, and 58; Alpha-7: HPV 18, 39, 45, 51, 59, and 68; and Alpha-5, 6: HPV 53, 56, 66, and 69), and the REBA HPV-ID assay, which detects 32 HPV genotypes based on the reverse blot hybridization assay (REBA) for the detection of oncogenic HPV infection according to cytological diagnosis. We also investigated the prevalence and genotype distribution of HPV infection with a total of 324 liquid-based cytology samples collected in western Shandong province, East China. Results: The overall HPV prevalences determined by HPV DNA and HPV E6/E7 mRNA assays in this study were 79.9% (259/324) and 55.6% (180/324), respectively. Although the positivity of HPV E6/E7 mRNA expression was significantly lower than HPV DNA positivity, the HPV E6/E7 mRNA assay showed greater specificity than the HPV DNA assay (88.6% vs. 48.1%) in normal cytology samples. The prevalence of Alpha-9 (HPV 16, 31, 33, 35, 52, and 58) HPV infection among these women accounted for up to 80.3% and 76.1% of the high-grade lesions detected in the HPV mRNA and DNA tests, respectively. The HR-HPV genotype distribution, based on HPV DNA and E6/E7 mRNA expression by age group in patients with cytologically confirmed lesions, was highest in women aged 40 to 49 years (35.9% for cytologically confirmed cases, Pearson correlation r value=0.993, p<0.001) for high-grade lesions. Among the oncogenic HR-HPV genotypes for all age groups, there was little difference in the distribution of HPV genotypes between the HPV DNA (HPV -16, 53, 18, 58, and 33) and HPV E6/E7 mRNA (HPV -16, 53, 33, 58, and 18) assays. HPV 16 was the most common HPV genotype among women with high-grade lesions. Conclusions: Our results suggest that the HPV E6/E7 mRNA assay can be a sensitive and specific tool for the screening and investigation of cervical cancer. Furthermore, it may provide useful information regarding the necessity for early cervical cancer screenings and the development of additional effective HPV vaccines, such as one for HPV 53 and 58. Additionally, gaining knowledge of HPV distribution may also inform us about ecological changes in HPV after the vaccination.

A Study on the Au Recoverability from Mongolian Tailings (몽골 광미로부터 Au 회수 가능성에 관한 연구)

  • Ko, Chin-Surk;Burentogtokh, Togtokhmaa;Lee, Jong-Ju;Park, Cheon-Young
    • Korean Journal of Mineralogy and Petrology
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    • v.33 no.1
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    • pp.41-51
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    • 2020
  • The purpose of this study was to investigate the possibility of eco-friendly/efficient recovery of valuable resources, such as Au from mine tailings, which are environmental pollutants in the Mongolian mine sector. For this purpose, this study selected 4 place of mine tailings of the Mongolian mines sector and carried out mineralogy evaluation of the valuable resources in the tailings. In this study, flotation was performed to separate and concentrate valuable resources in the tailings. Microwave nitric acid leaching was used to leach the valuable resources contained in the sample and to improve the Au grade. Chloride leaching attempted to leach Au from the leaching residues. XRD analysis of the tailings samples showed that most of the samples consisted of silicate minerals. As a result of confirming the content of the element through XRF analysis, the SiO2 content was very high, the Fe2O3 content was 2.32-4.23%, and the content of PbO, CuO and ZnO components were all within 2%. As a result of flotation for the tailings samples, the recovery of Au was the highest in Bayanairag sample (95.38%). As a result of microwave nitric solution experiment on Au concentrate sample obtained by flotation, the content of Au in the microwave nitrate leaching residue increased by 12.15% from 192.72 g/ton to 216.14g/ton in Khamo sample, the highest increase was 57.58% in Bayanairag sample. TCLP tests on tailings generated after flotation showed dissolution characteristics within EPA. Chloride leaching test was performed to recover Au from solid residues. The leaching rate was 87.43-89.35% within 10 minutes. For Khamo sample, 100% Au was leached after 60 minutes of leaching time. Therefore, in order to process the tailings continuously generated in Mongolia, applying the same process as the present study is expected to effectively recover the valuable resources contained in the tailings.

Introduction of an Electrofishing Technique for Assessments of Fish Assemblages to Korean Watersheds (우리나라의 수계에서 어류채집 효율성을 위한 전기충격기의 도입)

  • Hwang, Soon-Jin;Bae, Dae-Yeul;Lee, Jae-Yon;Kim, Ja-Hyun;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.38 no.4 s.114
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    • pp.482-493
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    • 2005
  • The objective of this research was to evaluate an sampling effect of fish species and individual number on sampling techniques of electrofishing and conventional capture methods of cast net and kick net in 38 stream sites sampled during June - September, 2005. For the study, sampling gears were categorized as three types of electrofishing method (EM), conventional sampling technique (CM), and the combined technique (CT) of the electrofishing and the conventional method to compare statistical differences. Major differences of species composition between the CM and EM method were found in some benthic species of Misgurnus mizolepis and Iksookimia koreensis along with lithophilic species of Pungtungia herzi. These species were predominated in the EM rather than the CM, indicating that conventional sampling can underestimate the abundance of benthic or lithophilic species. In contrast ,individual number of typical water column species such as Zacco platypus and Zacco temmincki were more sampled by the CM, so that these fish populations were community overestimated. Also, t-tests on the types of sampling gear from various watersheds of Chogang Stream, Yugu Stream, Daejeon Stream, and Gap Stream showed that total individual numbers and species number in each stream were significantly (t values = 2.806-6.896, p values<0.05, n = 5-14) greater in the CT than the CM. Similar statistical significance (p<1.001, n = (10-24) on sampling seasons were observed during the monsoon and postmonsoon. These results indicate that if the electrofishing is not added to the conventional gears, the abundance of fish population and community can be underestimated and some benthic or lithophilic species may be excluded from the analysis, resulting in overall errors including sampling, fish fauna, and final judgement of community abundance. Overall our results strongly suggest that new application of electrofishing method along with the conventional sampling gears reduce sampling bias on underestimation of the real fish populations and communities.