• Title/Summary/Keyword: Environmental Report

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Morphological and Molecular Characterization of Apharyngostrigea pipientis Faust, 1918 (Trematoda: Diplostomidea) from Ardeacinerea jouyi, Egretta intermedia and Nycticorax nycticorax in Korea

  • Kim, Hyeon-Cheol;Hong, Eui-Ju;Ryu, Si-Yun;Choi, Kyoung-Seong;Yu, Do-Hyeon;Cho, Jeong-Gon;Park, Jinho;Chae, Joon-Seok;Park, Bae-Keun
    • Journal of Veterinary Clinics
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    • v.37 no.5
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    • pp.242-248
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    • 2020
  • An intestinal strigeiid trematode, Apharyngostrigea pipientis, was detected from Ardea cinerea jouyi, Egretta intermedia and Nycticorax nycticorax between July, 2006 and June, 2020 in Daejeon metropolitan city and Kangwon-do, Republic of Korea. It was observed with a light and scanning electron microscope (SEM), and molecularly analyzed with 18S rRNA genes. The infection rate of the strigeiid trematode was 25.9% (7/27). The body is cylindrical in whole mount, greatly elongated in outline and shows a strong dorsal curvature. The overall measurements are: length 4.1-4.8 mm; fore-body 1.00-1.20 mm by 0.8-0.9 mm; hind-body 3.2-3.6 mm by 0.43-0.46 mm. The body is covered by a relatively thick cuticle, devoid of spines. The oral sucker leads into a short esophagus that is devoid of a muscular pharynx. The holdfast organ is enormously developed. The vitelline follicles distributed dorsal wall of fore-body and hind-body. This strigeiid trematode especially differs from other species of genus Apharyngostrigea in morphology. Although these A. pipientis are several morphological distinctions from Apharyngostrigea cornu, the partial sequence of 18S rRNA showed the sequence homology of A. pipientis (99.9%) and A. cornu (99.8%), and this result revealed their conspecific relationship between A. cornu and A. pipientis. This is the first report of A. pipientis in the Republic of Korea.

Chemical Composition and in vitro Antimicrobial and Antioxidant Activities of Commercially Available Essential Oils against Multidrug Resistant Bacteria (시판 정유의 성분 분석과 다약제 내성균에 대한 항균활성 및 항산화 효능 평가)

  • Yoo, Yeong Wol;Lee, Hyo Jeong;Kim, Seung;Bae, Min Suk;Lee, Mi Ja;Shim, Jung-Hyun;Cho, Seung Sik
    • Journal of Life Science
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    • v.24 no.3
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    • pp.266-273
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    • 2014
  • In the present study, the chemical compositions and antimicrobial and antioxidant activities of commercially available essential oils in Korea were investigated. The essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The results demonstrated that they exhibit a broad spectrum of antimicrobial activities against gram positive, gram negative, and multidrug resistant (MDR) strains. The antimicrobial activity of five of the essential oils against 40 organisms was assessed using the minimum inhibitory concentration (MIC). The MIC values were in the ranges of 0.0625-0.5% (v/w) for lemongrass and manuka, 0.03125-1.0% (v/w) for tea tree, 0.0625-1.0% (v/w) for thyme, and 1-4% (v/w) for ravensara, depending on the pathogens studied. This study revealed that, among the essential oils tested, lemongrass and thyme oil showed broad antimicrobial activity against infectious bacteria. The antioxidant activities and the reducing power of the essential oils were determined with a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. Thyme oil exhibited the strongest antioxidant activity comparing with ascorbic acid. This is the first report on the chemical compositions and antimicrobial activities of commercially available essential oils against infectious bacteria and MDR strains acquired from Korean hospitals.

Characteristics of Unit Load Generation and Discharge from Livestock Resources (축분자원화물 발생 및 배출부하 원단위 특성)

  • Han, Gee-Bong;Lee, Young-Siu;Lim, Jai-Myug
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.4
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    • pp.82-90
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    • 2008
  • The generation amounts of manure and urine were total 4.57kg/head/d of 1.49kg manure/head/d and 3.08kg urine/head/d with consideration of 3 seasons and live weight. The finalized mean unit load generation of filth were estimated at BOD 199.5g/head/d, CODCr 413.5g/head/d, T-N 27.8g/head/d, T-P 5.3g/head/d with consideration of seasons and the type of stalls. The unit load generation of manure and urine from korean cow were analyzed as 10.9kg/head/day and 3.3kg/head/day, respectively. The unit load generation of manure from milch cow was analyzed to be 24.6L/head/day which was similar to the report of MOE(2006). The urine generation, 10.5L/head/day, was higher than the results from MOA(2000) and NIAR(2000), but was lower than the results from MOE(2006), NIER(1986) and KIST(1990). The unit load generation from egg layers estimated by discharged amount and concentration analysis increases in due to the difference of planting head numbers, feed stuffs and manure disposal. In case of deer unit load generation by herbivora were calculated to be higher than data from existing references because of the gap between weight per livestock and the generation amounts of manure and urine.

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Comparative Analysis of Aniline Dioxygenase Genes from Aniline Degrading Bacteria, Burkholderia sp. HY1 and Delftia sp. HY99. (Aniline 분해균주 Burkholderia sp. HY1과 Delftia sp. HY99에서 유래된 Aniline Dioxygenases 유전자의 비교 분석)

  • Kahng, Hyung-Yeel;Oh, Kye-Heon
    • Microbiology and Biotechnology Letters
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    • v.35 no.2
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    • pp.104-111
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    • 2007
  • In this study, aniline dioxygenase genes responsible for initial catabolism of aniline in Burkholderia sp. HY1 and Delftia sp. HY99 were cloned and the amino acid sequences were comparatively analyzed, which already have been reported as bacteria utilizing aniline as a sole source of carbon and nitrogen, B. sp. HY1 was found to have at least a plasmid, and the plasmld-cured strain, B. sp. HY1-PC obtained using mitomycin C was tested with wild type strain to investigate whether the former maintained the degradability for aniline. This proved that the aniline oxygenase gene from B. sp. HY1 was located in chromosomal DNA, not in plasmid DNA. Aniline dioxygenase small subunits from B. sp. HY1 and D. sp. HY99 were found, based on 146 amino acids, to share 79% similarity. Notably, ado2 genes from B. sp. HY1 and D. sp. HY99 which were found to be terminal dioxygenase of aniline dioxygenase small subunit showed 99% similarity in the deduced amino acid sequences with tdnA2 of Frateuria sp. ANA-18 and danA2 of D. sp. AN3, respectively. Besides, enzyme assay and amino acid sequence analysis of catechol dioxygenase supported the previous report that B. sp. HY1 might occupy ortho-cleavage pathway using catechol 1,2-dioxygenase, while D. sp. HY99 might occupy catechol 2,3-dioxygenase for meta-cleavage pathway.

A Cost-Benefit Analysis on the Introduction of EU REACH to Korea (EU 신화학물질정책(REACH) 도입에 대한 비용편익 분석)

  • Cheong, Hoe-Seog
    • Journal of Environmental Policy
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    • v.8 no.3
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    • pp.53-79
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    • 2009
  • This study conducted a regulatory impact analysis regarding the introduction of the Korean version of REACH(Registration, Evaluation and Authorization of Chemicals). The direct cost of the Korean REACH is estimated at a total of 101 billion Korean won over the 11 year period. The cost includes pre-registration, testing, registration, Chemical Safety Assessment(CSA) and Chemical Safety Report(CSR), evaluation, and the authorization costs of 15,223 chemical substances produced and imported more than 1 ton per year in Korea in 2006. With regard to the benefit, the only public health benefit is included in the estimation. Based on the available foreign and domestic data, this study estimated that the economic values of public health benefits are in the range of 33.2~138.6 billion Korean won if only the savings of the National Health Expenditures are considered and it reaches 203.9~1,640.3 billion Korean won if the willingness to pay(WTP) for disease prevention is included. This study proved that the Korean REACH passed the cost/benefit criteria. The benefit-cost ratio of the Korean REACH, however, is estimated to be lower than its EU counterpart. Thus it is suggested that a rigorous study to reduce the costs to industry be required before the Korean government introduces the Korean REACH.

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Herpes Simplex Virus Infection after Corrective Rhinoplasty through External Approach: Two Case Reports (외접근 비교정술 후 발생한 단순 포진 바이러스 감염 치험례)

  • Kim, Hong Il;Hwang, So Min;Ahn, Sung-Min;Lim, Kwang Ryeol;Jung, Yong Hui;Song, Jennifer K.;Jeong, Jae Yong
    • Archives of Craniofacial Surgery
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    • v.13 no.1
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    • pp.68-71
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    • 2012
  • Purpose: Eczema herpeticum, caused by herpes simplex virus, is an infectious disease involving skin and internal organs. Varieties of physiologic, psychosocial, or environmental stress reactivate reservoir virus which exists in the trigeminal nerve ganglia. Authors report rare cases of nasal eczema herpeticum following corrective rhinoplasty. Methods: First case, 22-year-old female underwent corrective rhioplasty through an external approach in a local clinic. She developed progressive and painful erythema, nodules and vesicles on nose on the 9th day postoperatively. This unfamiliar lesion lead to a misdiagnosis as a bacterial infection, and had accelerated its progress to the trigeminal innervation of the nasal unit. Second case, a 23-year-old female underwent corrective rhinoplasty by external lateral osteotomy. Ten days after the surgery, disruption occurred on the external osteotomy site, and the ulceration gradually worsened. The surgeon misdiagnosed it as secondary bacterial infection and only an antibacterial agent was applied. Results: Both cases were healed effectively without any complication with proper wound dressing and antiviral therapy, and show no sequelae during an 8-month follow-up period. Conclusion: Eczema herpeticum is rare in the field of plastic surgery, but it should be kept in mind that secondary bacterial infections may lead to serious complications such as full-thickness skin loss. Thus, acknowledgement of the patient's past history regarding perioral or intraoral lesion may provide the surgeon with the possible expectancy of eczema herpeticum. Thus, if anyone develops eczema herpeticum, following facial plastic surgery, early diagnosis and immediate proper antiviral therapy will allow fast recovery without serious complications.

Ribonucleotide Reductase Activity of Schizosaccbarornyces pombe Is Inhibited by Escherichia coli RecA Antibody (Schizosaccbarornyces pombe에서 Escherichia coli RecA 항체에 의한 Ribonucleotide Reductase 효소활성 저해)

  • Lee, Jung-Sup;Chun, Min-Suck;Kim, Ok-Bong;Park, Jong-Kun;Kim, Si-Wouk;Park, Yeal;Yang, Young-Ki;Hong, Seung-Hwan;Park, Sang-Dai
    • The Korean Journal of Zoology
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    • v.38 no.4
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    • pp.565-569
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    • 1995
  • We have previoosly demonstrated that the RecA-like protein of Schizosaccharomyces pombe (S. pombe) is immunologically related to Escherichia coil (E. coil) RecA protein and that the cellular level of the protein is significantly increased by inhibitors of nucleotide pool-forming enzymes such as hydroxyurea (HU) and methotrexate (MTX) (lee and Park, 1994; lee et al., 1994). In this study, we report that the ribonudeotide redudase activity of S. pombe is inhibited by E. coil RecA antibody, as determined by thin layer chromatography using [5-$^3$H]CDP as a substrate. The relative activity of ribonucleotide reductase was dramatically inhibited by 100 mM of flu (26.4% reduction) in in vitro assay, compared to that of non-treated control. The ribonucleotide reductase activity was also inhibited by immunoprecipitation with E. coil RecA antibody (43.3% reduction). These results indicate that the strudure of S. pombe ribonucleotide reductase is in part similar to that of E. coil RecA protein.

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Forest Environment Degradation and Rehabilitation of Copper Mine Area in Ashio, Japan (일본 아시오(足尾) 銅鑛山地域의 삼림황폐와 삼림환경 복구사업에 관한 분석)

    • Korean Journal of Environment and Ecology
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    • v.15 no.3
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    • pp.276-285
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    • 2001
  • This report surveyed degradation of forest environment and rehabilitation in Ashio of Japan. Since 1880, a large scale forestry in this area has been destroyed by sooty smoke, and the local government invested heavily to rehabilitate the damaged forestry and denuded mountains. These degradations are due to complex operations, such as sulfurous acid gas from copper refinery, forest fires, steep slope and disadvantageous climate. The rehabilitation works on degraded forestry(2,399ha) were undertaken by tree planting fur three years from 1897. However, forest degradation and disasters were continued, and the total damaged areas were about 2,400~3,000ha in 1956. A Manual labor method, a Helicopter method and also Combination of manual labor and helicopter method had been adopted to rehabilitation works from 1945 to 1996, while 828.19ha of the degraded mountains was rehabilitated. Total investment for those projects was 80 billion yen. A debris control dam, a soil arresting structure, a vegetation-block, a vegetation sack measures and tree planting have implemented significantly fur the method of rehabilitation. An objective of manual labor works is a complete rehabilitation on each place through 3 stage working. The revived green areas accounted fur 49% of the total, and the entire afforest areas are less than 10%. In coming 25 years, an amount of 21.3 billion yen will be invested to rehabilitate 564ha of degraded mountain lands. However, it is impossible to estimate that how long it will take until the whole degraded mountain lands are completely rehabilitated. Rehabilitation works in Ashio may be applicable to environmental restoration and revegetation in the abandoned coal-mine lands of Korea.

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Developing Trends of the Chiral Drug Separation and Analysis Technology by using Molecular Recognition (분자인식 기법에 의한 키랄 의약품 분리 분석기술 개발동향)

  • Park, Gyung Hee;Lee, Yo-Han;Chang, Sang Mok;Kim, Woo-Sik;Kim, Jong-Min
    • Clean Technology
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    • v.22 no.2
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    • pp.75-81
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    • 2016
  • As the quality of life has improved, the desire for the safety and quality of the foods and drugs has been gradually increasing. For safety and quality management in foods, drugs, health management, agriculture, environmental conservation, and the industrial fields, the demand for quickly and accurately measuring various chemicals has been increasing. As well, the desire for self-diagnosis of one's own health state and self-examining the safety of environment has been gradually increasing. Optical Isomers can have very different physiological effects on human beings. One isomer can exhibit desirable pharmacological, pharmacodynamic, pharmacokinetic and physiological properties, while the other isomer can exhibit undesirable and toxic properties toward living organisms, especially human beings. And they can exhibit different activities in chemical and biotechnological processes. Although the majority of commercially available drugs are now both synthetic and chiral materials, a most chiral drugs are still marketed as racemic drugs. Thus, to avoid possible undesirable side effects from chiral drugs, more effective methods for separating and recognizing chiral compounds are urgently needed. In this report, we investigated the overall developing trends of the chiral drug separation and analysis technology by using molecular recognition.

Study on Market Prospects, Financing Challenges and Alternative Solutions in New Nuclear Power Projects (신규 원전의 시장전망 및 금융조달의 과제와 대안)

  • Lee, Jang-pyo
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.1
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    • pp.133-141
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    • 2016
  • Although construction of any new nuclear power projects had not been launched since mid-1970s until recently in the USA, many new nuclear power plants have been constructed in many countries with the support of their governments mainly as part of their national energy security and electric source diversification policies. For many reasons, the nuclear power industry seemed to reclaim their renaissance from the beginning of this century and the investment in the nuclear power projects draw positive concern from the private financial sector. But the global financial crisis in 2008 and subsequent economic slow-down together with tighter bank credit regulations caused commercial banks, the main source of financing, to lose appetite for investing in new nuclear power projects. But the nuclear power economics shows that the nuclear power is viable in terms of the environmental benefit and long-term average cost compared to other power generation sources. Also doubt about nuclear power safety was much mitigated due to technology development and reinforced safety-related tests and monitoring. Therefore, the prospect for nuclear power market expansion remains positive although there are comparatively big differences among different scenarios. After Korea Electric Power Corp. won the UAE nuclear power project in December of 2009, the competition in nuclear power markets is undergoing huge changes. Competitors backed by the support of their own governments are now entering the market with many aggressive and innovative financing packages to win bids of new nuclear power projects. This report analyzed the nuclear power market prospects, competitive edges of nuclear power, risk management measures, and financing challenges and recommends alternative solutions to promote competitive edges in winning bids of new nuclear power projects.