• Korean Journal of Microbiology
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• v.28 no.1
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• pp.6-12
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• 1990

The preS2 wequence of an adr hepatitis B virus was cloned and expressed in Escherichia coli as a $\beta$-galactosidase fusion polypeptide. Recombinant preS2 product interacted with the preS2-specific monoclonal antibody H8 which was induced by surface antigen particles isolated from a Korean gepatitis patient. The H8 showed only a minor cross-reactivity with recombinant preS2 product of adw2 subtype. Determination of nucleotide sequence of the adr preS2 revealed that twelve amino acid residue substitutions between adr and adw2 subtype sequences. The antigenic determinant to H8 must include some of these differences.

• Structural Engineering and Mechanics
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• v.56 no.6
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• pp.917-938
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• 2015

The nonlinear numerical analysis of the impact response of reinforced concrete/mortar beam incorporated with the updated Lagrangian method, namely the Smoothed Particle Hydrodynamics (SPH) is carried out in this study. The analysis includes the simulation of the effects of high mass low velocity impact load falling on beam structures. Three material models to describe the localized failure of structural elements are: (1) linear pressure-sensitive yield criteria (Drucker-Prager type) in the pre-peak regime for the concrete/mortar meanwhile, the shear strain energy criterion (Von Mises) is applied for the steel reinforcement (2) nonlinear hardening law by means of modified linear Drucker-Prager envelope by employing the plane cap surface to simulate the irreversible plastic behavior of concrete/mortar (3) implementation of linear and nonlinear softening in tension and compression regions, respectively, to express the complex behavior of concrete material during short time loading condition. Validation upon existing experimental test results is conducted, from which the impact behavior of concrete beams are best described using the SPH model adopting an average velocity and erosion algorithm, where instability in terms of numerical fragmentation is reduced considerably.

• Journal of Microbiology
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• v.35 no.3
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• pp.213-221
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• 1997

The secretion of the alkaliphilic Bacillus sp. S-1 extracellular pullulanase involves translocation across the cytoplasmic membrane of the Gram-positive bacterial cell envelope. Translocation of the intracellular pullulanase PUL-I, was traced to elucidate the mechanism and pathway of protein secretion from an alkaliphilic Bacillus sp. S-1. Pullulanase could be slowly bue quantitatively released into the medium during growth of the cells in medium contianing proteinase K. The released pullulanase lacked the N-terminal domain. The N-terminus is the sole membrane anchor in the pullulanase protein and was not affected by proteases, confirming that it is not exposed on the cell surface. Processing of a 180,000M$\_$r/ pullulanase to a 140,000M$\_$r/ polypeptide has been demonstrated in cell extracts using antibodies raised against 140,000M$\_$r/ extracellular form. Processing of the 180,000 M$\_$r/ protein occured during the preparation of extracts in an alkaline pH condition. A modified rapid extraction procedure suggested that the processing event also occured in vivo. Processing apparently increased the activity of pullulanase. The western blotting analysis with mouse anti-serum against 140-kDa extracellular pullulanase PUL-E showed that PUL-I is processed into PUL-X via intermediate form of PUL-E. Possible explanationa for the translocation are discussed.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.11
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• pp.1143-1149
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• 2008

In a nuclear power plant, loose part monitoring and its diagnostic technique is one of the major issues for ensuring the structural integrity of the reactor system. Typically, accelerometers are mounted on the surface of a reactor vessel to localize impact location cavsed by the impact of metallic substances on the reactor system. However, in some cases, the number of the accelerometers is not enough to estimate the impact location precisely. In such a case, one of alternative plan is to utilize another type sensors that can measure the vibration of the reactor structure even though the measuring frequency ranges are different from each others. The AE sensors installed on the reactor structure can be utilized as additional sensors for loose part monitoring. In this paper, we proposed a new method to estimate impact location by using both accelerometer signal and AE signal, simultaneously. The feasibility of the proposed method is verified by an experiment. The experimental results demonstrate that we can enhance the reliability and precision of the loose part monitoring.

• Journal of the Korean Society of Propulsion Engineers
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• v.9 no.2
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• pp.89-96
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• 2005

This study has been performed on the mechanism of heat transfer between stratospheric airship and its surroundings while the airship is staying in the air at the altitude of 20km. The computational grid of airship has been generated and the results influenced by the number and the shape of grids have been compared. The temperature distributions have been obtained through this thermal analysis considering three modes of heat transfer - conduction, convection and radiation - in stratospheric conditions. Based on the airship's surface and inner temperature variations, the influence of temperature distributions on the helium envelope and the payload has been predicted.

• Geomechanics and Engineering
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• v.7 no.2
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• pp.213-231
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• 2014

An elastoplastic model for structured clays, which is formulated based on the fact that the difference in mechanical behavior of structured and reconstituted clays is caused by the change of fabric in the post-yield deformation range, is present in this paper. This model is developed from an elastoplastic model for overconsolidated reconstituted clays, by considering that the variation in the yield surface of structured clays is similar to that of overconsolidated reconstituted clays. However, in order to describe the mechanical behavior of structured clays with precision, the model takes the bonding and parabolic strength envelope into consideration. Compared with the Cam-clay model, only two new parameters are required in the model for structured clays, which can be determined from isotropic compression and triaxial shear tests at different confining pressures. The comparison of model predictions and results of drained and undrained triaxial shear tests on four different marine clays shows that the model can capture reasonable well the strength and deformation characteristics of structured clays, including negative and positive dilatancy, strain-hardening and softening during shearing.

• Korean Journal of Materials Research
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• v.31 no.8
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• pp.450-457
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• 2021

From measured thermal conductivity and modeling by simulation, this study suggests that U-factors are highly related to materials used between steel and polymer. The objective and prospective point of this study are to relate the relationship between the U-factor and the thermal conductivity of the materials used. For the characterization, EDX, SEM, a thermal conductive meter, and computer simulation utility are used to analyze the elemental, surface structural properties, and U-factor with a simulation of the used material between steel and polymer. This study set out to divide the curtain wall system that makes up the envelope into an aluminum frame section and entrance frame section and interpret their thermal performance with U-factors. Based on the U-factor thermal analysis results, the target curtain wall system is divided into fix and vent types. The glass is 24 mm double glazing (6 mm common glass +12 mm Argon +6 mm Low E). The same U-factor of 1.45 W/m²·K is applied. The interpretation results show that the U-factor and total U-value of the aluminum frame section are 1.449 and 2.343 W/m²·K, respectively. Meanwhile, those of the entrance frame section are 1.449 and 2.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.3
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• pp.509-520
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• 2023

In this paper, the structure and the characteristics of the Fly-By-Wire system applied to the civil aircraft was described. The development trend of the redundant method of the flight control system, data communication system, control surface actuation system and the control laws implemented by the Fly-By-Wire system of the civil aircraft are discussed. The Fly-By-Wire system was first applied to the fighter and its inherent advantages lead to the advent of the Fly-By-Wire civil aircraft. Recently even the small jet aircraft shows the trend of adopting the Fly-By-Wire system. In the future, most of the aircraft are expected to be the Fly-By-Wire type.

• BMB Reports
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• v.29 no.2
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• pp.175-179
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• 1996

Viral surface proteins are known to play an essential role in attachment of the virus particle to the host cell membrane. In case of the hepatitis B virus (HBV) several reports have described potential receptors on the target cell side, but no definite receptor protein has been isolated yet. As for the viral side, it has been suggested that the preS region of the envelope protein, especially the preS1 region, is involved in binding of HBV to the host cell. In this study, preS1 region was recombinantly expressed in the form of a maltose binding protein (MBP) fusion protein and used to identify and visualize the expression of putative HBV receptor(s) on the host cell. Using laser scanned confocal microscopy and by FACS analysis, MBP-preS1 proteins were shown to bind to the human hepatoma cell line HepG2 in a receptor-ligand specific manner. The binding kinetic of MBP-preS1 to its cellular receptor was shown to be temperature and time dependent. In cells permeabilized with Triton X-100 and treated with the fusion protein, a specific staining of the nuclear membrane could be observed. To determine the precise location of the receptor binding site within the preS1 region, several short overlapping peptides from this region were synthesized and used in a competition assay. In this way the receptor binding epitope in preS1 was revealed to be amino acid residues 27 to 51, which is in agreement with previous reports. These results confirm the significance of the preS1 region in virus attachment in general, and suggest an internalization pathway mediated by direct attachment of the viral particle to the target cell membrane.

• KIEAE Journal
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• v.3 no.2
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• pp.37-44
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• 2003

The aim of this study was to analyze the thermal performance through Test-Cell of TI-wall in domestic climate. This study was carried out as follows: 1) The TI-wall was studied for ability to reduce heat loss through the building envelope and analyzed to TIM properties. 2) Test models of TI-wall were designed through the investigation of previous paper and work, measured for winter and spring, and the thermal effects were analyzed. The type of the TIM used in test model is small-celled(diameter 4mm and thickness 50mm) capillary and cement brick(density $1500kg/m^3$) was used by thermal mass. 3) Test-cell of TI-wall was calibrated from measured data and the dynamic simulation program ESP-r 9.0. In these simulations, the measured climate conditions of TaeJon were used as outdoor conditions, and the simulation model of Test-cell was developed. 4) The sensitivity analysis is executed in various aspects with standard weather files and ESP-r 9.0, and then most suitable system of TI-wall are predicted. Finally, The suitable system of TI-wall was analysed according to sizes of air gap, kinds, thickness, and the surface absorption of therm wall. The result is following. In TI-wall, Concrete is better than cement brick, at that time the surface absorption is 95%, and the most efficient thickness is 250mm. As smaller of a air gap, as reducer of convection heat loss, it is efficient for heating energy. However, ensuring of a air gap at least more than 50mm is desirable for natural ventilation in Summer.