• Applied Microscopy
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• 제50권
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• pp.13.1-13.7
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• 2020

We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15-19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58 ± 0.85 ㎛ (n = 20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.

• 천문학회보
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• 제45권1호
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• pp.42.4-43
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• 2020

The hydrogen-rich envelope mass of a dying massive star is the key factor that determines the type and properties of the resulting supernova. Emulating wind-driven mass loss of single stars with the MESA(Modules for Experiments in Stellar Astrophysics) stellar evolution code, we made a grid of models for a large parameter space of initial mass (12 M⊙ to 30M⊙), metallicity (solar, LMC and SMC), hydrogen envelope mass (0.01M⊙ to 10M⊙) for progenitor stars in their final step of evolution. Our results suggest the final luminosity of the progenitor is largely determined by the initial mass, which means there is luminosity degeneracy for stars with the same initial mass but with different hydrogen-rich envelope masses. Since we can break this degeneracy by correcting luminosity with surface gravity (spectroscopic HR diagram), we can infer the exact mass property of an observed progenitor. The surface temperature drastically varies near the envelope mass of ~0.1M⊙ and surface temperature of ~10000 K, where the demarcation between the hydrogen-rich envelope and the helium core lies, which explains the rarity of 'white' supergiants. There also exists a discontinuity in the chemical composition of the progenitor envelope around this critical hydrogen-rich envelope mass of ~0.1 M⊙, which can be tested in future observations of "flash spectroscopy" of supernovae.

• Applied Microscopy
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• 제41권1호
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• pp.13-20
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• 2011

바다빙어목(Osmeriformes), 바다빙어과(Osmeridae)에 속하는 빙어(Hypomesus nipponensis)의 성숙란 난막구조를 광학현미경과 전자현미경을 이용하여 관찰하였다. 빙어의 성숙란은 두 개의 난막으로 둘러싸여 있었으며 바깥쪽 난막의 외층은 한 층의 여포세포가 분포하고 있었고 안쪽의 난막 바깥쪽은 호염기성을 나타내는 구조물들이 배열하고 있었다. 난막의 단면을 투과전자현미경으로 관찰한 결과 두 개의 난막 중 바깥쪽 막은 전자밀도가 균일한 한 층으로 되어 있었고 안쪽막은 전자밀도가 높은 6층이 전자밀도가 낮은 5층에 의해서 분리되어 층상구조를 하고 있었다. 주사전자현미경으로 관찰한 결과 바깥쪽 막은 홈들이 관찰되었고 안쪽막 표면은 무정형의 구조물들이 산재하고 있었다. 또한 동물극 쪽에 한 개의 난문이 관찰되었다. 이상과 같이 빙어의 성숙란은 지금까지 보고된 경골어류의 경우 한 개의 난막으로 둘러싸여 있는데 비해서 두 개의 난막으로 둘러싸여 있다는 것은 매우 중요한 빙어의 종특이성을 나타내며 난막의 미세구조적 특징들은 종을 분류하는 데 이용될 수 있다.

• Applied Microscopy
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• 제52권
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• pp.6.1-6.5
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• 2022

We examined the morphology of the fertilized egg and the fine structure of fertilized egg envelopes of Poropanchax normani belonging to the family Poeciliidae, also known as Norman's lampeye using light and electron microscopes. The fertilized eggs with narrow perivitelline space were found to be spherical and demersal, additionally containing small oil droplets in the vitelline membrane. Further, a bundle of adhesive filaments was observed to be present on one side of the fertilized egg. These filaments possessed remarkably high elasticity and were approximately 1-3mm in length. The size of the fertilized egg was determined to be about 1.49 ± 0.07mm (n=30). The outer surface appeared smooth, and adhesive filaments originating at different location of the surface of the envelope were found to be distributed around the egg envelope and were joined together to form a single long bundle in scanning electron microscopic observation. A peak-like structure formed of several straight wrinkles was observed around the micropyle. However, the complete structure of the micropyle could not be studied due to the depth at which it was located. Additionally, the total thickness of the egg envelope was ascertained to be approximately12.5-14.5㎛. The egg envelope consisted of two distinct layers, an outer electron dense layer and an inner lamellar layer, further consisting of 10 sublayers of varying thicknesses. Collectively, it was observed that the morphological characteristics of the fertilized egg, fine structures surrounding the micropyle, outer surface, adhesive structure consisting adhesive filaments, and sections of fertilized egg envelope displayed species specificity.

• Applied Microscopy
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• 제30권1호
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• pp.61-72
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• 2000

U. unicinctus 난자를 대상으로 인공수정 후 수정막 형성에 따른 물질적 기원과 첨체반응 등에 관련된 구조변화에 대하여 광학현미경과 전자현미경을 이용하여 관찰하였다. 관찰결과에 의하면 1차 수정막의 물질은 과잉의 정자에 대한 효과적 방어 기능을 보유한 난외막 표층 기원의 물질이었으며, 2차 수정막은 피질과립의 분비에 의하여 형성되었다. 3차 수정막은 광학현미경하에서 특이한 구조의 관찰은 없었으나 전자현미경에 의한 관찰시 많은 입자상 물질들로 구성되었으며, 수정 후 60분에는 이들 입자물질의 응집에 의해서 수정막은 경과되는 것으로 관찰되었다. 1차 수정막과 난외막 표층의 유사성 그리고 1차 수정막의 형성과 미세융모의 퇴화가 동시적인 사건이라는 것은 미세융모가 U. unicinctus에서 정자의 수용체로 작용할 수 있는 것으로 추정할 수 있었다. 첨체소관에 의한 난세포막 침투시 침체 단부에서 과립상의 물질이 표층(surface coat)에 확산되었다. 첨체소관은 첨체의 기부에서 시작되고, 첨체간격을 통하여 신장되었다.

• 한국컴퓨터그래픽스학회논문지
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• 제8권4호
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• pp.1-7
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• 2002

본 논문에서는 3차원 인체 형상 스캔 데이터로부터 팔, 다리형상을 복원하는 방법을 제시한다. 이 방법에서는 팔, 다리 스캔 데이터의 대략적인 형상을 나타내는 기반 곡면과 자세한 세부 현상을 나타내는 displacement 맵의 이중구조로 형상을 복원한다. 팔, 다리 부분의 스캔 데이터 형상은 골격을 따라 스윕하는 타원체로 근사되며, 이 타원체 스윕을 부드럽게 감싸는 envelope 곡면으로 기반 곡면을 생성한다. 타원체 스윕의 envelope 곡면은 빠른 계산을 위해 골격을 따라 추출되는 타원의 스윕 곡면으로 근사된다. 기반 곡면에 대한 스캔 데이터 점들의 displacement는 각 단면 타원으로의 매핑을 통해 스칼라 값으로 구해지며, 다단계 스플라인 함수를 이용하여 매개화된 displacement 맵을 구성한다. 이 과정에서 복원된 형상 위의 점들은 해당하는 타원체 상으로 매핑된다. 본 방법을 통하여 팔, 다리의 간결한 형상 표현을 추출할 수 있으며, 매핑된 타원체를 이용하여 형상을 빠르고 사실적으로 변형할 수 있다.

• Applied Microscopy
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• 제45권3호
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• pp.144-149
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• 2015

Nothobranchius guentheri and Nothobranchius patrizii have special life cycle to sustain the dry season. So, we investigated the fertilized eggs morphology, and compared ultrastructures of surface structures and the cross section of fertilized egg envelopes using light and electron microscopes to determine whether these fertilized eggs and egg envelopes show the species specificity or have special structure to sustain the dry season. These fertilized eggs were spherical, yellowish, demersal and adhesive, and had a one-sided large oil droplet. The whip-like structures, adhesive filament were distributed throughout egg envelope in both species. But, that of N. guentheri was covered with fibrous structures, and that of N. patrizii was smooth. The egg envelope consisted of two distinct layers: an outer, electron-dense layer containing adhesive filaments and an inner layer of 16 to 17 horizontal electron-dense lamellae alternating with 15 to 16 interlamellae of lower electron density in both species. The external shapes of fertilized egg and section of fertilized egg envelope were same, but ultrastructure of adhesive filaments on the outer surface was concluded to show species specificity. Our data indicate that the ultrastructural differences of adhesive filament and outer surface of fertilized egg envelope show species specificity although these species belong to same genus.

• 한국어류학회지
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• 제13권1호
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• pp.19-23
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• 2001

미꾸리과 어류 2종 수수미꾸리 Niwaella multifasciata, 좀수수치 Kichulchoia brevifasciata의 성숙란에 대하여 난막구조를 조사하였다. 성숙란의 난막 (egg envelope)은 부착구조 (adhesive structure)가 부착하는 zona radiata externa와 여러 전자밀도층을 가지는 zona radiata interna층으로 구성되어 있다. 수수미꾸리의 zona radiata externa는 짧은 융모형태 (short villus-like protuberance)의 구조를 가지며 길이는 약 1.5~2.5 ${\mu}m$로서 2.0~2.5 ${\mu}m$ 간격으로 배열하고 있었다. 반면에 좀수수치는 물결모양 형태 (wave-like structure)를 가지는데 길이는 약 1.5~2.5 ${\mu}m$ 크기로 서로 2.5~3.3 ${\mu}m$ 간격으로 배열되어 있다.

• Animal cells and systems
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• 제5권1호
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• pp.45-50
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• 2001

Rana ovarian follicles consist of oocyte, vitelline envelope, granulosa cells, and theca/epithelial layer. Using scanning electron microscopy, the surface structure of each follicular component was investigated. Changes in oocyte surface during oocyte maturation were also examined. Theca/epithelial layer was almost transparent and some blood vessels and granulosa cells were observed underneath in intact follicle. The number of granulosa cells was estimated to be 6700-7200 per oocyte. The granulosa cells partially overlapped each other and their microvilli penetrated the vitelline membrane via holes present in the vitelline envelope and seemed to be linked to oocyte microvilli. After removal of the vitelline envelope by microforcep, oocyte microvilli were observed on the surface of the devitellined oocyte. The oocyte microvilli formed partial clusters on the surface of white spot area which appears iust before germinal vesicle breakdown (GVBD), whereas they were evenly distributed in other areas. The microvilli became shorter and less dense with oocyte maturation. The lengths of oocyte microvilli in the immature and mature oocyte were 1.5 $\mu$m and 0.6 $\mu$m, respectively. The present study suggests a fundamental structural change occurring on the oocyte surface during maturation.

• 국제학술발표논문집
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• The 5th International Conference on Construction Engineering and Project Management
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• pp.553-557
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• 2013

Roof and exterior wall are designed and constructed in a manner that prevents the accumulation of water within the wall and roof assembly in the formal building. However, in a freeform building there is no clear distinction between exterior wall and roof. In other words, the exterior walls and roof systems of the freeform building are integrated as a surface, unlike the formal building envelope. Therefore, freeform architecture needs a systemized envelope design method to perform functions of exterior wall and roof. However, in many cases, construction methods for roof and exterior wall are applied to freeform buildings without necessary alterations, which lead to incomplete design, leakage, cracks and other problems. Freeform architecture is thus designed and constructed differently from formal buildings. In order to more easily and inexpensively actualize freeform architecture, Building Information Modeling (hereinafter referred to as BIM) has recently been applied in the construction industry. The studies and case analysis are not sufficient to identify the implications and contributions of freeform buildings in future similar projects. Therefore, this research will study design and construction methods for freeform surfaces. This study attempts to analyze the pros and cons of each method for the concrete surface frame, and then presents the panel options for envelope system of the freeform architecture.