• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.321-334
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• 2017

Process surveillance within agricultural biogas plants (BGPs) was concurrently studied by high-throughput 16S rRNA gene amplicon sequencing and an optimized quantitative microscopic fingerprinting (QMF) technique. In contrast to 16S rRNA gene amplicons, digitalized microscopy is a rapid and cost-effective method that facilitates enumeration and morphological differentiation of the most significant groups of methanogens regarding their shape and characteristic autofluorescent factor 420. Moreover, the fluorescence signal mirrors cell vitality. In this study, four different BGPs were investigated. The results indicated stable process performance in the mesophilic BGPs and in the thermophilic reactor. Bacterial subcommunity characterization revealed significant differences between the four BGPs. Most remarkably, the genera Defluviitoga and Halocella dominated the thermophilic bacterial subcommunity, whereas members of another taxon, Syntrophaceticus, were found to be abundant in the mesophilic BGP. The domain Archaea was dominated by the genus Methanoculleus in all four BGPs, followed by Methanosaeta in BGP1 and BGP3. In contrast, Methanothermobacter members were highly abundant in the thermophilic BGP4. Furthermore, a high consistency between the sequencing approach and the QMF method was shown, especially for the thermophilic BGP. The differences elucidated that using this biphasic approach for mesophilic BGPs provided novel insights regarding disaggregated single cells of Methanosarcina and Methanosaeta species. Both dominated the archaeal subcommunity and replaced coccoid Methanoculleus members belonging to the same group of Methanomicrobiales that have been frequently observed in similar BGPs. This work demonstrates that combining QMF and 16S rRNA gene amplicon sequencing is a complementary strategy to describe archaeal community structures within biogas processes.

• Journal of Life Science
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• v.20 no.7
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• pp.1073-1077
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• 2010

Fungi, including allergenic, pathogenic or toxigenic species, may have direct effects on human health, interfere with the disinfection process and maintenance of chlorine residual, and add to taste and odor problems. We surveyed distribution of waterborne fungi from raw water in Busan metropolitan city using the EPA standard method. According to the survey, conducted from March 2008 to October 2008, the number of fungi in raw water was high in September and low in April. Quantitative enumeration of fungi per 100 ml of raw water ranged from 5 to 64 CFU, and a higher number was detected in the Mulgum region compared to the Maeri region. Total fungi numbers highly correlated with pH, water temperature and total coliform in this area. Predominant fungi genera grown in medium were identified as Aspergillus, Penicillum, Histoplasma, Rhizopus, Trichophyton, Mucor and Sporthrix species. Aspergillus flavus and Penicillum notatum were particularly dominant in both areas. There were a total of 6 and 9 species detected in the Mulgum and Maeri regions respectively, and the number of species was more verified in the Maeri region than the Mulgum region.

• (The) Research of the performance art and culture
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• no.40
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• pp.163-192
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• 2020

Sinjungsin Mask Play, one of Ttangseolbeop, is related to Seongjusin's life story. Sinjungsin Mask Play is a reconstruction of the story of the folk gods Seongjusin met while returning home. Seongjusin's life story proceeds in the form of Mask Play, and the monk who leads the sermon plays narration and main roles. Many believers play various roles and musicians. Sinjungsin Mask Play introduces many folk beliefs, sounds for intrigue, and talks. Sinjungsin Mask Play uses the same method of enumeration and repetition as the existing Mask Play. The repetition of a sentence or phrase plays a role in foreseeing the meaning of the context or foretelling the development of the plot to the audience. This repetition is intended to emphasize the situation of the scene and to create rhythm. Since Mask Play was exclusively for the common people, Mask Play actors use the repeating method commonly used in folk songs to form lines. This gives the audience a familiarity, effectively communicating the lines and responding to their tastes. Sinjungsin Mask Play borrowed people's way of playing for the public's mission. It inherits the dramatic forms of traditional traditional plays such as repetition of words or sentences or phrases, codification of words or sentences, borrowing of existing songs, and formal expression units. In addition, through repeated performances, believers can easily and easily learn and understand. This is the dramatic form and characteristics of Sinjungsin Mask Play. Sinjungsin Mask Play was handed down from Faith Communities and was used as a means of folk cultivation to spread illegality. Buddhism externalizes the process of accepting folk beliefs through Mask Play, and in the case of Shinto who participated directly or indirectly, they naturally acquire the belief system of Hwaeom Kyung through play. Sinjungsin Mask Play, one of Ttangseolbeop, can be said to have great value as an ICH, as well as popularization and mission.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.3
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• pp.60-67
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• 2016

In cumulative transfer functions, as number of transfer increase, the impact of individual transfer to transfer cost increase linearly or non linearly. This function can effectively explain various passengers's travel behavior who choose their travel routes in integrated transit line networks including bus and railway modes. Using the function, it is possible to simulate general situations such that even though more travel times are expected, less number of transfer routes are preferred. However, because travel cost with cumulative transfer function is known as non additive cost function types in route search algorithms, finding an optimal route in integrated transit networks is confronted by the insolvable enumeration of all routes in many cases. This research proposes a methodology for finding an optimal path considering cumulative transfer function. For this purpose, the reversal phenomenon of optimal path generated in route search process is explained. Also a heuristic methodology for selecting an optimal route among multiple routes predefined by the K path algorithm. The incoming link based entire path deletion method is adopted for finding K ranking path thanks to the merit of security of route optimality condition. Through case studies the proposed methodology is discussed in terms of the applicability of real situations.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.185-192
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• 2013

BACKGROUND: As a result of rural exodus, aging of rural population, development of high-yield rice varieties and so on, abandoned paddy fields of South Korea have generally increased. The phytosociological studies are needed to recommend the effective vegetation management methods of abandoned paddy fields. The current study performed an analysis of vegetation characteristics by classifying plant communities for abandoned paddy fields in South Korea. METHODS AND RESULTS: A comparative analysis of syntaxonomy, synecology, and syndynamics was performed by using the Z.-M. method of phytosociology and vegetation survey which was obtained from an enumeration district of total 203 phytosociological releves of 5 regions in Gangwon-do, 5 regions in Gyeonggi-do, 2 regions in Chungcheong-do, 18 regions in Gyeongsang-do, and 10 regions in Jeolla-do. Alopecurus aequalis community, Juncus effusus var. decipiens community and Salix koreensis community were, respectively, identified as successional plant communities of the early stage, the middle stage, and the late stage in the vegetation of abandoned paddy field. Sere of abandoned paddy field can be classified, developmentally, into annual plant stage, perennial plant stage, shrub plant stage, and forest tree stage. Several kinds of dominant species rather than a distinct species were found in the middle stage of abandoned paddy field. CONCLUSION(S): In this study, changing pattern in vegetational communities of abandoned paddy field were understood. These results will provide important information for restoring and conserving the ecology of fallow ground.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.586-599
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• 2017

This study attempts to understand the overall research trends in Vocational Studies using a text mining method, which is a means to analyze big data. The findings of the research show that Vocational Studies in Korea has been directly influenced by global economic crises, as evidenced by its exponential growth after the 1997 foreign exchange crisis that resulted in a bailout from the IMF. In addition, the topics of research have been shifting from such macro subjects as government policies and systems to such micro topics as individual career development. Moreover, the perspective of research is being moved from the socially vulnerable, including women and the disabled, to the economically marginalized, including retirees and the unemployed. As for the research targets, college students overwhelmingly outnumbered primary and secondary school students. However, few cases analyzed the clinical outcomes of career counseling or attempted to process job information and study the history of jobs. This research is limited in that it only analyzed journal abstracts. Nonetheless, it is meaningful because it used topic analysis, one of the text mining methods, to give a complete enumeration of all articles available for search, thereby crafting a framework of quantitative analysis methodology for Vocational Studies. It is also significant in that it is the first attempt to analyze themes in every stage of the development of Vocational Studies.

• Journal of dental hygiene science
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• v.15 no.4
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• pp.495-502
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• 2015

In this study, it was to understand the factors influencing choice conviction of dental hygiene major graduate school of dental hygienist. This study aims to provide the basic data for developing the dental hygienic profession. The study method was complete enumeration of the master's graduate 84 students majoring in dental hygiene in the country 8 graduate school. The results of this study, the analysis of the differences between the external factors and internal factors of graduate school choice, factors of financial accessibility factor related marriage factor. Factor of the education program were more unmarried. The average monthly income was found to have recognized high financial accessibility of factors, education program. Also the carrier is low, factor of the education program it was found to be high. The result of graduate school choice conviction, the more unmarried chosen choice conviction score in high, it choice conviction score was related to monthly income. The result of correlation analysis, external reputation, future, education program if high, it was found to have conviction of graduate school. In graduate school choice conviction external factors of education program and future factor was found to influence the choice of graduate school but internal factors was not found to influence. Therefore, we must seek the support measures that can deepen the sustainable development and dental hygiene of the dental hygienist.

• Journal of Korean Society of Transportation
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• v.21 no.2
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• pp.95-105
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• 2003

Because the basic assumptions of deterministic user equilibrium assignment that all network users have perfect information of network condition and determine their routes without errors are known to be unrealistic, several stochastic assignment models have been proposed to relax this assumption. However. it is not easy to solve such stochastic assignment models due to the probability distribution they assume. Also. in order to avoid all path enumeration they restrict the number of feasible path set, thereby they can not preciously explain the travel behavior when the travel cost is varied in a network loading step. Another problem of the stochastic assignment models is stemmed from that they use heuristic approach in attaining optimal moving size, due to the difficulty for evaluation of their objective function. This paper presents a logit-based stochastic assignment model and its solution algorithm to cope with the problems above. We also provide a stochastic user equilibrium condition of the model. The model is based on path where all feasible paths are enumerated in advance. This kind of method needs a more computing demand for running the model compared to the link-based one. However, there are same advantages. It could describe the travel behavior more exactly, and too much computing time does not require than we expect, because we calculate the path set only one time in initial step Two numerical examples are also given in order to assess the model and to compare it with other methods.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• Korean Journal of Food Science and Technology
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• v.41 no.1
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• pp.11-15
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• 2009

Commercial tofu was cut and re-packed in a vacuum container, an airtight container and an open tofu tray in an effort to assess the effects of vacuum containers on the shelf life of leftover tofu. The re-packed tofu were stored at $5^{\circ}C$ and $25^{\circ}C$ for the enumeration of total bacteria and coliforms. We also measured changes in color, pH, and opacity. The vacuum retarded the bacterial growth under $10^5$ CFU/mL for up to 12 days at $5^{\circ}C$. The color of the tofu was unaffected by the container type at both storage temperatures, whereas the pH and opacity of the immersion solution were altered less profoundly by storage in the vacuum container at $5^{\circ}C$. However, we noted no significant differences in bacterial growths, pH, and opacity among container types at $25^{\circ}C$. The results of this study showed that the usage of vacuum containers may be a favorable method for the preservation of tofu under refrigerated conditions.