• Title/Summary/Keyword: Enumeration method

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Development of DNA-Based Assessment Method for Mold in Floor Dust of Dwellings in Korea (바닥 먼지내에서 DNA 기반 곰팡이 분석기법 개발)

  • Lee, Jeong-Sub;Kim, Sung Yeon;Choi, Kil Yong;Ryu, Jungmin;Hwang, Eun Seol;Lee, Juyeong;Kwon, Myunghee;Chung, Hyenmi;Seo, SungChul
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.27 no.4
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    • pp.324-332
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    • 2017
  • Objectives: Much scientific evidence indicate a positive association between moldy environments and respiratory illnesses and/or symptoms. However, few comprehensive assessments of mold have been performed for such settings. Spore counts or microscopic enumeration only may not be sufficient for evaluating fungal exposure. Recently, Mold Specific QPCR technology developed by the US EPA (Environmental Relative Moldiness Index, ERMI) has been widely used worldwide and great performance for assessing fungal exposure has been shown. Methods: We aimed to develop a Korean version of ERMI suitable for the distribution of fungal flora in Korea. Thirty dwellings in the Seoul and Incheon area were selected for sampling, and each was classified as 'Flooded, 'Water-damaged' or 'Non-water-damaged'. Results: Dust on the floor and airborne sampling were collected using an MAS100 and a 'Dustream' collector. Samples were analyzed by quantitative polymerase chain reaction(QPCR) for the 36 molds belonging to ERMI. Student t-test and ANOVA tests were carried out using SAS software. The median ERMI values of flooded, water damaged, and non-water damaged dwellings were 8.24(range: -5.6 to 27.9), 5.47(-25. 4 to 32.7), and -15.30(-24.6 to 14.8), respectively. Significant differences were observed between flooded and non-water damaged dwellings (P=0.001) and between water-damaged and non-water damaged dwellings (P=0.032). Conclusion: Our findings indicate that ERMI values attributed to dust samples in Korea could be applicable for the identification of flooded or water damaged buildings. However, much data is needed for continuously developing the Korean version of ERMI values.

Comparison of Various Methods Applied to the Leukocyte Differential Counting (각종백혈구백분비계산법(各種白血球百分比計算法)에 관(關)한 고찰(考察))

  • Yoon, Young Hun
    • Korean Journal of Veterinary Research
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    • v.1 no.1
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    • pp.30-35
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    • 1961
  • Inasmuch as a great variety of methods for the leukocyte differential counting have been described, it may be expected that actual counts obtained considerably vary according with the particular methods applied to the enumeration of differentiated leukocyes. An attempt, therefore, was undertaken to compare the values measured by five authentic methods commonly in use with those obtained by thirteen methods devised by the author. The results seemed to indicate that relatively reliable method is to count, end to end, all leucocytes on fields looted on the central horizontal axis of the film and two adjacent lines parallel to the central line and quartering the film.

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Comparison of sampling method of phytoplankton for type approval of ballast water management system (선박평형수처리장치 형식승인을 위한 식물플랑크톤 샘플링 방법 비교)

  • Jang, Pung-Guk;Hyun, Bonggil;Lee, Woo-Jin;Choi, Keun-Hyung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.12
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    • pp.426-433
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    • 2020
  • This study aimed to compare the pretreatment methods of phytoplankton for type approval of the Ballast Water Treatment System (BWMS). The International Maritime Organization (IMO) and the United States Maritime Police (USCG) use two different test methods for this purpose. To compare the two methods, a test for concentration and non-concentration was performed with cultured and natural phytoplankton, and samples from the land-based BWMS test. The extent of damages caused by the process of concentration varied between cultured and natural species, indicating differences depending on the physiological and morphological characteristics of the species. In the land-based test, in the control water with a high biological population, the number of non-concentrated samples was about twice as high as that of the concentrated samples. There was no distinct difference between the two methods in the treated water with a low biological population. Thus, although there is a difference between concentration and non-concentration for phytoplankton sampling, the concentration method can be applied as a method of evaluating BWMS performance. However, a method for evaluating whether live species in treated water may be lost or damaged during the concentration process of sampling should be developed and validated.

Improvement of Infiltration Performance Measurement in BTL (Build-Transfer-Lease) Sewer Rehabilitation Projects - Focusing on Jeju Special Self-Governing Province - (하수관거정비 임대형민자사업에 있어 침입수 성과지표의 개선에 관한 연구 - 제주특별자치도를 중심으로 -)

  • Ko, Young-Nam;Lee, Dong Wook
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.36 no.6
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    • pp.1133-1143
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    • 2016
  • The purpose of this study is to improve project performance analysis indicators for BTL sewer rehabilitation projects. Among the assessment indicators for BTL sewer rehabilitation projects, an infiltration assessment indicator is given a high score of 17.5 points as a single assessment item. This infiltration assessment indicator is assessed focusing on the amount of infiltration, and presently calculated according to 'Nighttime Domestic Flow Evaluation' method. However, this assessment indicator's failure to reflect the geological features of Jeju region is emerging as a problem in the operational stage. Thus, this study intended to compare and analyze the calculation result depending on the assessment indicators and the actual amount of infiltration, centering on Jeju region. To this end, this study analyzed the amount of infiltration in five areas of Jeju Province calculated according to 'Nighttime Domestic Flow Evaluation' method. Also, a complete enumeration survey was carried out about the conditions for actual infiltration occurrence. According to the results of this survey, ground water level is distributed lower than the level of sewer pipes. The results of a sewer pipe function test show there was no infiltration occurrence caused by sewer pipe defect. So, it is concluded that 'Nighttime Domestic Flow Evaluation' method, which is utilized for the current assessment indicator, is not appropriate to apply to Jeju region, and it is thought that there is a need to establish infiltration criteria specialized for Jeju region.

A Study on Brown's Subject Classification (브라운의 주제분류법 연구)

  • Kwak, Chul-Wan
    • Journal of the Korean Society for information Management
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    • v.35 no.4
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    • pp.37-50
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    • 2018
  • The purpose of this study is to analyze the first edition of Brown's Subject Classification and to understand the implications of today's library classification. For this purpose, the first edition of the Subject Classification published in 1906 was analyzed. The analysis results are divided into three main areas. First, SC is divided into eleven main classes and each class is subdivided into enumerated subdivisions from 000 to 999. Second, As a method of synthesizing the classification numbers, there were three methods of synthesis. There was a flexibility to insert a new classification number at the appropriate location when a new topic that does not appear in the main table appeared. Implications for classification studies can be divided into four main categories. First, SC proposed a method of classification number synthesis for composite topics, which is an innovative method that was not available in previous library classification. Second, the subject matter was explained in various aspects through the operation of auxiliary tables supporting the facets. Third, it is possible to easily insert a new topic into the classification system by using the SC that can have a flexible classification system for each library, or to use a short classification number according to the size of the library collection. Fourth, it provided a directory that can be considered as access points for digital materials.

Blood Picture of Army Pack Horses(Jeju Horses) (군용타마(軍用馱馬)(제주마(濟州馬))의 혈액상(血液像))

  • Mun, Gyeong Gi;Lee, Do Pil;Chung, Soon Tong
    • Korean Journal of Veterinary Research
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    • v.15 no.2
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    • pp.147-152
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    • 1975
  • Although considerable research has been done on the blood picture of the horse, hot-blooded and cold-blooded, little work has been made of the blood picture of the army pack horse, Jeju horse. The object of the present investigation was to make good this deficiency, and to suggest standard for the blood picture of army pack horses kept under the regular military training and the ideal feeding in the heart of a mountain. Blood samples were drawn from the jugular vein through a 15-gauge bleeding needle from 41 males and 28 females, aging 3 to 9 years old. It was taken between seven and nine o'clock in the morning. Animals were handled as quietly as possible to avoid any excitation. No restraint other than a halter was used. Enumeration of erythrocyte, total and differential leukocyte count, determination of hemoglobin in blood, and the value of packed cell volume were male in the usual manner, and erythrocytic constant was calculated by the method of Wintrobe. Erythrocyte count was $7.83{\pm}0.20(4.95{\sim}11.05){\times}10^6/mm^3$(SE). This value was much lower than hot-horses, but slightly higer than the values of cold-horse reported from foreign country. Concentration of hemoglobin in blood was $13.0{\pm}0.33(9.5{\sim}17.8)g/100ml$. This value was much higher than that of cold-horses observed by the other authors, approaching to the values of hot-horses. Packed cell volume was $32.1{\pm}0.92(22{\sim}42)ml/100ml$. This vague was a little higher than that of the other cold-horses. Mean corpuscular volume was $41.5{\pm}1.20(26.6{\sim}59.3){\mu}m^3$. This value matched so well with the other results recorded by various investigators. Mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were $16.9{\pm}0.43(12.3{\sim}25)$ pg and $41.0{\pm}0.45(29.1{\sim}51.1)g/100ml$, respectively. These values were significantly higher than the values found by the other investigations. Total leukocyte enumeration was $10.5{\pm}0.41(5.6{\sim}17.9){\times}10^3/mm^3$, being considered as normal. And differential leukocyte count of neutrophil was $44.5{\pm}2.23(15{\sim}76)%$, $5,527{\pm}234(2,231{\sim}9,144)/mm^3$, of lymphocyte $50.5{\pm}1.19(19{\sim}77)%$, $4,307{\pm}125(1,456{\sim}11,098)/mm^3$, of monocytel (0~4)%, $105(0{\sim}352)/mm^3$, of eosiophil 3.2(0~14)%, $340(0{\sim}1,232)/mm^3$ and of basophil 0.25(0~3)%, $23(0{\sim}236)/mm^3$. The percentage of the differential count obtained from the present work showed a good agreement with the results of various authors. Of the horses examined monocyte was found from 42 horses, eosinophil from 62 horses and basophil from 10 horses. No significant differences recognized between male and female horses, and the effect of age was not observed between three to nine years old. Judging from the blood picture of the present investigation, it could be stated that the army pack horses on training were kept better than the average farming conditions.

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Effects of Areal Interpolation Methods on Environmental Equity Analysis (면내삽법이 환경적 형평성 분석에 미치는 영향)

  • Jun, Byong-Woon
    • Journal of the Korean association of regional geographers
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    • v.14 no.6
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    • pp.736-751
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    • 2008
  • Although a growing number of studies have commonly used a simple areal weighting interpolation method to quantify demographic characteristics of impacted areas in environmental equity analysis, the results obtained are inevitably imprecise because of the method's unrealistic assumption that population is evenly distributed within a census enumeration unit. Two alternative areal interpolation methods such as intelligent areal weighting and regression methods can account for the distributional biases in the estimation of impacted populations by making use of additional information about the geographic distribution of population. This research explores five areal interpolation methods for estimating the population characteristics of impacted areas in environmental equity analysis and evaluates the sensitivity of the outcomes of environmental equity analysis to areal interpolation methods. This study used GIS techniques to allow areal interpolation to be informed by the distribution of land cover types, as inferred from a satellite image. in both the source and target units. Independent samples t-test statistics were measured to verify the environmental equity hypothesis while coefficients of variation were calculated to compare the relative variability and consistency in the socioeconomic characteristics of populations at risk over different areal interpolation methods. Results show that the outcomes of environmental equity analysis in the study area are not sensitive to the areal interpolation methods used in estimating affected populations, but the population estimates within the impacted areas are largely variable as different areal interpolation methods are used. This implies that the use of different areal interpolation methods may to some degree alter the statistical results of environmental equity analysis.

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A Hybrid Dasymetric Mapping for Population Density Surface using Remote Sensing Data (원격탐사자료를 바탕으로 인구밀도 분포 작성을 위한 하이브리드 대시메트릭 지도법)

  • Kim, Hwa-Hwan;Choi, Jin-Mu
    • Journal of the Korean Geographical Society
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    • v.46 no.1
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    • pp.67-80
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    • 2011
  • Choropleth mapping of population distribution is based on the assumption that people are uniformly distributed throughout each enumeration unit. Dasymetric mapping technique improves choropleth mapping by refining spatially aggregated data with residential information. Further, pycnophylactic interpolation can upgrade dasymetric mapping by considering population distribution of neighboring areas, while preserving the volumes of original units. This study proposed a combined solution of dasymetric mapping and pycnophylactic interpolation to improve the accuracy of population density distribution. Specifically, the dasymetric method accounts for the spatial distribution of population within each census unit, while pycnophylactic interpolation considers population distribution of neighboring area. This technique is demonstrated with 1990 census data of the Athens, GA. with land use land cover information derived from remotely-sensed imagery for the areal extent of populated areas. The results are evaluated by comparison between original population counts of smaller census units (census block groups) and population counts of the grid map built from larger units (census tracts) aggregated to the same areal units. The estimated populations indicate a satisfactory level of accuracy. Population distribution acquired by the suggested method can be re-aggregated to any type of geographic boundaries such as electoral boundaries, school districts, and even watershed for a variety of applications.

Methane Production and T-RFLP Patterns of Methanogenic Bacteria Dependent on Agricultural Methods (농법에 따른 메탄생성과 메탄생성 세균의 T-RFLP 패턴)

  • Kim, Hun-Soo;Cho, Ju-Sik;Park, Kyeong-Ryang
    • Korean Journal of Microbiology
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    • v.45 no.1
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    • pp.17-25
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    • 2009
  • We studied soil components, methane production, the number of methanogens, and T-RFLP patterns dependent on agricultural methods with the change of seasons. There is no regular increase or decrease tendency of the most soil components followed by sampling period. And the water content in soil was higher in October than May. Also a lot of methanogens existed in soil, and acetotrophs were relatively of smaller number than hydogenotrophs and formate utilizing methanogens using MPN (most probable number) enumeration. In the experiment using the formate, it was used from the first week, and only a minute amount was detecte after four weeks. However in the acetate, it was increased until the third week, and after that was consumed. And there was higher methane production for all soil samples which administered with the hydrogen spike. The activity of methanogens was higher in the organic and low-agrichemical agricultural method samples, and the organic agricultural method had high methanogen activity among the other samples. A result of T-RFLP pattern of mcrA gene digested with Sau96I, methanogen community have a little relation with agricultural methods and seasons. This results also agreed to no critical difference the soil components dependent on agricultural methods, but some analytical data have a positive relationship with a agricultural methods. Therefor we could concluded that the comparison study of community for soil bacteria sufficiently could be useful for the microbiological indicator.

Application of Epifluorescence, Microscopy for Measurement of Bacterial Population in Water Supplies (용수중(用水中) 세균계수(細菌計數)를 위한 형광검경법(螢光檢鏡法)의 응용(應用))

  • Rhee, Young-Hwan;Shin, Seung-Yee
    • Korean Journal of Environmental Agriculture
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    • v.1 no.1
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    • pp.48-52
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    • 1982
  • Methods for the measurement of aquatic bacteria can be divided into two groups. The first group of these methods is based on the 'replicon' concept that live bacterial cells, when diluted and transferred to a suitable medium, produce colonies. These methods distinguish living from dead bacteria, but they massively underestimate bacterial numbers. The second group of enumeration methods uses visual counting technique using specific apparatus such as a microscope. These methods are generally direct and simple, but it is very hard to distinguish between live and dead bacteria and between small particle and bacteria. Recently developed technique in staining methods has provided a reliable method of visual determination of aquatic bacteria. This uses epifluorescence microscopy to measure the total bacterial population. In order to present the fluorescence microscopy as a new methodology for the determination of bacterial numbers in water supplies, data were obtained from chlorine and monochloramine doses added to samples. Total counts by fluorescence microscopy were compared with standard plate count method. The total number of bacteria in water supplies can be determined with fluorescence microscopy. This technique allows better resolution of small bacteria and differentiation of particle from bacteria. Chloramine was found to persist longer in natural waters and prevent bacterial regrowth.

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