• 한국환경보건학회지
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• 제31권1호
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• pp.23-30
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• 2005

Staphylococcus aureus is one of the important pathogenic agents, which are related to the hygienic condition. This study performed for the detection of Staphylococcus aureus and screening staphylococcal enterotoxin a, b, c genes in strains isolated from the environment for production of non-pasteurized strawberry juice. A total of 44 samples were collected from utensils, machinery, employees, raw materials, and strawberry juices in 3 strawberry juice shops in Jinju, western Gyeongnam. The isolation rate of Staphylococcus aureus was 26%. Specially Staphylococcus aureus was frequently isolated from employee's hands, strawberry and strawberry juices. The sea, seb, and sec genes were also investigated by polymerase chain reaction (PCR). One hundred and 55% of each isolate had found sea gene and seb gene, respectively. However, sec gene was not detected anywhere. To prevent food-borne disease associated with juice, the accomplishment of HACCP to be more efficient and systematic is necessary.

• 대한미생물학회지
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• 제21권1호
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• pp.47-52
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• 1986

A heat-labile enterotoxin and no heat-stable enterotoxin producing($LT^+ST^-$) plasmid (110 kilobases in size) was isolated from an enterotoxigenic Escherichia coli of human strain O15:H11 and used for analysis of the $LT^+$ deoxyrionucleic acid region using recombinant DNA technology. A DNA segment containing the $LT^+$ DNA region which was one restriction endonuclease BamHl fragment(6.2 kb in size) was joind to a small multicopy plasmid, pUC9. E. coli K-12 strain, JM103 harboring the chimeric plasmid produced greater amounts of LT than did the enterotoxigenic E. coli O15:H11 strain. The BamHl fragment was further digested with various restriction endonucleases and contained no HindIll restriction site which is an essential in $LT^+ST^+$ plasmid. The detailed DNA sequencing of this $LT^+ST^-$ plasmid is required.

• Food Science and Biotechnology
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• 제16권4호
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• pp.621-625
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• 2007

Staphylococcus aureus in Korean kimbab rolls was monitored seasonally in 4 major cities of Korea to investigate the risk of S. aureus in a pre-prepared meal. Thirty-five (28.6%) of 105 kimbab rolls purchased in winter were contaminated with S. aureus with an average level of 2.6 log CFU/g. Thirty-six (33.0%) of 109 kimbab rolls purchased in summer and autumn were contained S. aureus with an average level of 2.9 log CFU/g. Kimbab purchased in snack bars showed higher S. aureus contamination rates with the maximum level of 4.7 log CFU/g than that purchased in convenience stores. Of the raw materials in kimbab, uncooked perilla leaf had the highest contamination rate of S. aureus. Less than 50% of S. aureus isolated from kimbab produced enterotoxin and most of the staphylococcal enterotoxin produced by S. aureus in kimbab was type A.

• Journal of Magnetics
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• 제21권1호
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• pp.141-147
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• 2016

Staphylococcus aureus cultures exposed to rotating magnetic field (RMF) were studied in order to analyse the possible induced changes in staphylococcal enterotoxin genes (se) expression. Liquid cultures of S. aureus strains carrying different se were exposed to the RMF of magnetic frequency 50 Hz and magnetic induction 34 mT for 10 h at $37^{\circ}C$. Three time points of bacterial growth cycle were considered for RNA extractions. Gene expression analyses were evaluated using real-time quantitative PCR method. The present study confirmed, that the RMF can stimulate the growth rate of S. aureus cultures in comparison to the unexposed controls, while the stimulation is not strain dependent. The studies have also shown, that the RMF, depending on the exposure time but regardless the bacterial strain, can influence on the expression of various se. In general, except for sea, as a result of bacterial exposure to the RMF through subsequent growth phases, the expression of se decreased, reaching the values below results recorded for unexposed controls. In the case of sea expression remained at a lower level as compared to the control, regardless the time of exposition.

• 한국식품과학회지
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• 제38권2호
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• pp.295-303
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• 2006

본 연구는 서부 경남지역 토마토 농장 5 곳에서 총 120점의 시료를 수집하여 S. aureus를 분리하였으며, 분리된 S. aureus 에대하여 PCR에 방법을 이용, 내독소 유전자(enterotoxin genes)와디스크 확산방법(disk diffusion method)을 이용하여 22가지 항생제에 대하여 항생제 감수성 실험을 수행하였다. 본 연구의 결과 14점의 시료(11.7%)가 S. aureus에 오염되어 있었다. 가장 빈번하게 검출된 시료로는 관개용수, 양액, 작업자의 손 그리고 토마토였다. 또한 14개의 시료에서 enterotoxin 유전자가 검출되었고, 이들 중 sea 유전자를 갖는 균주는 2주(14.3%), sea와 sed 유전자를 동시에 갖는 균주가 3주(21.4%), sea, sed 및 see 유전자가 모두 검출된 균주는 7주(50.0%)였다. 그러나 sec, sed와 tsst유전자를 갖는 균주는 검출되지 않았다. 분리된 균주를 대상으로 항생제 내성실험결과 penicillin에 대한 항생제 내성이 64.3%로 뚜렷하게 높은 것으로 나타났다. 그 외 novobiocin(57.1%)에 대하여 8 균주, amphicillin(42.9%)에 대하여 6균주, erythromycin(13.4%), oxacillin (14.2%)에 대하여 2 균주, kanamycin(7.2%), doxycycline(7.2%)에대하여 1 균주가 내성을 나타내었다. 특히 B 농장의 롤러와 D 농장의 양액에서 분리된 균주는 oxacillin에 내성을 갖는 MRSA(Methicillin Resistant S. aureur)균주였다. 따라서 본 연구결과는 안전 농산물 생산을 위한 미생물학적 정보를 제공할 수 있을 것으로 사료되는 바이다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권6호
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• pp.671-677
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• 1996

Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination (MSHA) and mannose-resistant hemagglutination (MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-labile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28 (3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable (STaI and STaII) and heat-labile (LT) enterotoxin genes in E. coli.

• 한국식품위생안전성학회지
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• 제6권2호
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• pp.79-81
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• 1991

1990년 5우러 23일 27열의 식중독이 보고되었다. 그들은 미국 New Jersey 주의 Gergen Country에 있는 한 회사의 개업기념 야유회에 참석한 직원과 가족들 중 일부였다. 섭취한 음식에 대한 설문조사로 ziti 가 전파매체였던 것으로 생각되었다. (Chi Square 9.05) 잠복기는 9시간, 증세 시속시간은 평균 24시간이었다. 대변검사결과 Clostridium perfringens와 엔테로톡신이 분리되었다.

• 대한의생명과학회지
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• 제11권3호
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• pp.295-299
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• 2005

Staphylococcus aureus is an important animal and human pathogen implicated in a variety of disease including food-poisoning caused by staphyloccal enterotoxins (SEs). In order to investigate the difference in genomic types and to monitor the transmission of S. aureus isolates, a total of 25 S. aureus isolates from different sources were determined for their genotypic characteristics by pulsed-field gel electrophoresis (PFGE) in addition to their ability to enterotoxin production and antibiotic resistance patterns in this study. All the isolates were susceptible to amikacin, and the resistance pattern to ampicillin and penicillin were most common among 14 different patterns. Eleven of 24 isolates produced one of three SEs, SEA, SEC or SED. Sixteen representative PFGE patterns were obtained by Smal restriction fragments of S. aureus isolates. Analysis of dendrogram based on PFGE band patterns suggested that food-poisoning outbreaks be caused by the diverse sources of food, of which their raw materials were infected with S. aureus. Also, it could be concluded that PFGE was a powerful tool for epidemiological tracing of infection source for food-initiated outbreaks.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.305.2-305
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• 1998

No Abstract, See Full Text

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.617-618
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• 2005