• Journal of Yeungnam Medical Science
• /
• 제20권2호
• /
• pp.129-141
• /
• 2003

Background: Leptin is a 16-KDa non-glycosylated peptide hormone synthesized almost exclusively by adipocytes. The well-known function of leptin is regulation of food intake and energy expenditure. Leptin also plays a regulatory role in immune and inflammatory process including cytokine production. The purpose of this study was to investigate the effect of leptin on the expression of several chemokine genes(RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$) in THP-1 cells. Materials and Methods: Total RNA of THP-1 cells were prepared by Trizol method, and then stimulated with the leptin(250 ng/$m{\ell}$) or LPS(100 ng/$m{\ell}$). We examined the expression patterns of various chemokine mRNAs in THP-1 cell lines by RT-PCR and Northern blot. Results: Leptin did not induce the expression of chemokine mRNAs in THP-1 cells. The expression patterns of RANTES, IL-8, MCP-1, IP-10, and Mig mRNAs in THP-1 cells stimulated with leptin and LPS simultaneously was almost same to the patterns of LPS alone-induced chemokine mRNAs. RANTES mRNA expression was independent on the concentrations of leptin. Although leptin did not have strong effect on the expression of RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$ mRNAs in THP-1 cells, leptin could induce the expression of long isoform of leptin receptor(OB-RL) mRNA, and its expression was elevated in simultaneous stimulation of leptin and LPS. Conclusion: These data suggest that leptin is able to induce OB-RL in THP-1 cells, however, leptin has little effect on the expression of pro-inflammatory chemokine genes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권8호
• /
• pp.1062-1068
• /
• 2004

Leptin has a major role in the regulation of food intake and energy homeostasis. In addition, leptin participates in many physiological functions including regulation of lipid metabolism. Bovine recombinant leptin protein was produced in E. coli cells in order to understand function of leptin in the regulation of lipid metabolism. The leptin expression vector was constructed in pGEX-4T-3 vector and transformed into E. coli BL21 cells. Expression of the GST-leptin fusion protein was induced with IPTG. The fusion protein was purified using glutathione sepharose 4B batch method, and the recombinant leptin was eluted after thrombin protease digestion. The effect of leptin on glucose transport was examined in the differentiated adipocytes of 3T3-L1 cells. Leptin had no effect on basal and insulin-stimulated glucose transport in 3T3-L1 cells (p>0.05). Effect of recombinant leptin on glucose and acetate transport was examined in adipose tissues of Korean cattle (Hanwoo). Insulin stimulated glucose transport in both intramuscular and subcutaneous adipose tissues (p<0.05), but leptin did not affect glucose transport in both adipose tissues (p>0.05). Insulin stimulated acetate transport in bovine adipose tissues (p<0.05), but leptin did not affect acetate transport (p>0.05). Northern and RT-PCR analyses showed that mRNA levels of uncoupling protein-2 were increased by leptin treatment in 3T3-L1 cells without statistical difference (p>0.05). In conclusion, bovine recombinant leptin did not affect glucose and acetate transport in both 3T3-L1 adipocytes and bovine adipose tissues, while it stimulates UCP-2 mRNA expression in 3T3-L1 cells.

• 한국위성정보통신학회논문지
• /
• 제10권1호
• /
• pp.49-55
• /
• 2015

인지무선 네트워크에서 스펙트럼 센싱은 우선사용자에게 간섭을 주지 않기 위해 기본적으로 수행해야 하는 단계이다. 스펙트럼 센싱에 요구되는 샘플 수는 2차 사용자의 성능에 직접적으로 영향을 주기 때문에, 2차 사용자의 성능과 우선사용자에 대한 간섭은 트레이드오프 관계에 있다. 스펙트럼 센싱에 필요한 샘플 수는 요구되는 오검출 확률, 검출확률 및 우선 사용자의 최소 요구 SNR로 부터 얻어진다. 우선 사용자 센싱에 요구되는 SNR은 2차 사용자의 전송반경과 관련 있기 때문에, 2차사용자들을 모아 센싱집합으로 구성하고 요구되는 전송영역을 최소화시킴으로써 스펙트럼 센싱에 요구되는 우선사용자의 SNR을 완화시킬 수 있다. 따라서 스펙트럼 센싱에 필요한 최소 샘플 수를 줄임으로써 인지무선 네트워크의 전송량을 향상시킬 수 있다. 본 논문에서는 이를 위해 센싱집합인 클러스터링을 통해 게임이론으로 클러스터의 크기에 따라 얻는 이득과 손실을 트레이드오프로 디자인하고, 시뮬레이션을 통해 제안된 방법의 성능을 확인한다.

• 펄프종이기술
• /
• 제36권3호
• /
• pp.15-34
• /
• 2004

Diverse benefits such as reduction of fresh water consumption and effluent discharge, efficient use of raw materials and energy savings can be obtained by papermaking system closure. Closure of papermaking processes, however, causes many problems including reduction of the efficiency of additives, decrease of retention and dewatering, felt plugging, poor Paper quality, generation of slime and odor, poor vacuum efficiency, etc, and it has been recognized that accumulation of Inorganic and organic substances in the process white water is the prime cause of these problems. Therefore, technological developments for preventing accumulation of these detrimental substances are urgently required for Implementing papermaking system closure. Understanding of the accumulation phenomena of the inorganic and organic substances in the papermaking process white water is prerequisite for papermaking system closure. In this study a process simulation method was used to analyze the accumulation phenomena of anionic starch In the process white water as the closure level of a fine paper making process is increased. A pilot paper machine was used as a model process. Starch adsorption and desorption models were developed based on the concept of starch adsorption ratio, which was not considered in previous studies. Steady state simulation studies were carried out based on this model using a commercial simulator. In steady state simulation, the variation of dissolved starch concentration in each process unit was monitored as a function of white water usage for wire shower. The result of the steady state simulation showed that dissolved starch concentration and its increase ratio in Process units increased as white water usage ratio for wire shower increased.

• Fisheries and Aquatic Sciences
• /
• 제1권1호
• /
• pp.48-62
• /
• 1998

Seasonal volume transport on the Texas-Louisiana continental shelf is investigated in terms of objectively fitted transport streamfunction fields based on the current meter data of the Texas­Louisiana Shelf Circulation and Transport Processes Study. Adopted here for the objective mapping is a method employing a two-dimensional truncated Fourier representation of the streamfunction over a domain, with the amplitudes determined by least square fit of the observation. The fitting was done with depth-averaged flow rather than depth-integrated flow to reduce the root-mean-square error. The fitting process filters out $11\%$ of the kinetic energy in the monthly mean transport fields. The shelf-wide pattern of streamfunction fields is similar to that of near-surface velocity fields over the region. The nearshore transport, about 0.1 to 0.3 Sv $(1 Sv= 10^6\;m^3/sec)$, is well correlated with the seasonal signal of along-shelf wind stress. The spring transport is weak compared to other seasons in the inner shelf region. The transport along the shelf break is large and variable. In the southwestern shelf break, transport amounts up to 4.7 Sv, which is associated with the activities of the encroaching of energetic anticyclonic eddies originated in Loop Current of the eastern Gulf of Mexico. The first empirical orthogonal function (EOF) of streamfunction variability contains $67.3\%$ of the variance and shows a simple, shelf-wide, along-shelf pattern of transport. The amplitude evolution of the first EOF is highly correlated (correlation coefficient: 0.88) with the evolution of the along-shelf wind stress. This provides strong evidence that the large portion of seasonal variation of the shelf transport is wind-forced. The second EOF contains $23.7\%$ of the variance and shows eddy activities at the southwestern shelf break. The correlation coefficient between the amplitudes of the second EOF and wind stress is 0.42. We assume that this mode is coupled a periodic inner shelf process with a non-periodic eddy process on the shelf break. The third EOF (accounting for $7.2\% of the variance) shows several cell structures near the shelf break associated with the variability of the Loop Current Eddies. The amplitude time series of the third EOF show little correlation with the along-shelf wind.

• 한국항해항만학회:학술대회논문집
• /
• 한국항해항만학회 2005년도 춘계학술대회 논문집
• /
• pp.385-390
• /
• 2005

LMTT(Linear Motor-based Transfer Technology)는 항만 자동화를 위한 수평 이송 시스템이며, 셔틀카(shuttle car)와 격자구조의 레일에 부착된 스테이터 모듈(stator module)로 구성된 PLMSL(Permanent Magnetic Linear Synchronous Motor)에 의해 구동된다. 본 논문에서는 LMTT시스템 부품인 이동체(mover)의 경량화를 위하여 직교배열표 및 크리깅 방법을 이용하여 최적설계를 수행한다. 설계변수로는 가로빔, 세로빔, 휠빔의 두께와 높이 방향의 치수를 결정하는 형상변수를 포함시켰다. 목적함수로는 중량, 제한조건 함수로는 안전율이 고려된 응력으로 설정하였다. 본 연구에서 제시된 방법으로 구한 최적해를 민감도기반 최적설계로 구해진 최적해와 비교, 검토하였다.

• 복식
• /
• 제57권2호
• /
• pp.1-10
• /
• 2007

This study aims at the improvement of sewing function through understandings of dynamic property about the sewing methods and the thread material selection in knitwear. The tensile strength and shear of KES-FB and the Instron were measured for the analysis of the mechanical properties. The knit cloth was structured In the plain stitch, $1\times1$ rib stitch and $2\times1$ rib stitch with the combination of wool and cotton. With regard to the sewing method, intralooping and interlacing were applied. For thread materials, polyester, cotton, wool and silk were used. Since silk has the lowest extension and similar values regardless of its construction in intralooping, it is available knit apparel with uniform elastic recoverv. It also has small shearing resistance. It can be used in apparel which needs big mobility, but it causes rutting problem. Therefore, it is suitable to use intralooping. When the same sewing yarn and textile are use, it can lower shearing resistance and extension in intralooping, Since wool needs a lot of extension energy and it can be cut, intralooping is more suitable than interlacing in sewing of wool. In interlacing using polyester, extension and shearing resistance are high. Therefore, it is suitable for knit sewing with high massing. Silk is not suitable for interlacing since it can be rut. Even though knit materials are different, the RT values of polyester and cotton are similar in same construction. Therefore, they can be substituted each other considering resilience after sewing.

• 한국정밀공학회지
• /
• 제30권7호
• /
• pp.769-776
• /
• 2013

The dynamic compliance characteristics of a prosthetic foot midgait are very important for natural performance in an amputee's gait and should be in a range that provides natural, stable walking. In this study, finite element analysis (FEA) and classical laminate theory were used to examine the mechanical characteristics of a carbon-epoxy composite laminate prosthetic foot as a function of variation in the lamination composition. From this analysis, an FEM model of a prosthetic keel, made from the composite material, was developed. The lamination composition of the keel was designed for improved stiffness. The prototype product was fabricated using an autoclave. Vertical loading response tests were performed to verify the simulation model. The results of the experiments were similar to those from simulations below the loading level of the gait, suggesting use of the proposed simulation model for prosthetic keel design.

• 대한전자공학회논문지SP
• /
• 제47권1호
• /
• pp.1-8
• /
• 2010

얼굴의 윤곽선을 검출하기 위해서는 일반적으로 입력 영상에 직접 동적 윤곽선 모델(Active Contour Model)을 적용하는 방법을 많이 사용한다. 그러나 동적 윤곽선 모델은 초기의 위치 설정과 사용되는 에너지 함수의 계수 값에 따라 성능에 영향을 받기 때문에, 다양한 조명조건과 환경조건에 따라 최적화된 파라미터들을 설정해야 하는 번거로움이 있다. 또한 섬세한 윤곽선의 검출을 위해서는 모델에서 사용되는 정점의 수를 증가시켜야 하는 단점이 있다. 이러한 단점들을 해결하기 위해, 본 논문에서는 입력영상의 분할된 영역에서의 히스토그램 분석을 통하여, 얼굴 영역과 배경 영역의 픽셀 값을 구분할 수 있는 임계값을 자동으로 찾아, 얼굴의 윤곽선 영역을 검출하는 접근 방법을 제안한다. 제안된 방법은 입력 영상의 분석을 통하여 얼굴의 윤곽선 영역을 검출하기 때문에 다양한 조명과 배경 조건하에서도 높은 성능으로 얼굴의 윤곽선 영역을 검출하였다.

• 한국태양에너지학회:학술대회논문집
• /
• 한국태양에너지학회 2012년도 춘계학술발표대회 논문집
• /
• pp.96-101
• /
• 2012

An experimental study was conducted to investigate the performance of a concentrating photovoltaic cell (CPV) against temperature. It is know that a high efficiency of a CPV can be achieved only with proper cell temperature as well as high concentration ratio (CR). This study is concerned with appropriate cooling condition for a liquid-convection cooler for the best performance of a specific CPV. A series of experiments was conducted in a range of cell temperatures as a result of varying cooling conditions, while the concentration ratio was 390 and the solar irradiation flux was higher than 900 $W/m^2$ in outdoor environment. The CPV had a planar dimension of 10 by 10 mm. A Fresnel lens was used as a concentrator, of which the dimension was 221 mm(W) ${\times}$ 221 mm(L) ${\times}$ 3 mm(t) and the transmissivity was known to be 0.8. The cooler was attached to the bottom side of the CPV and had a contact area of 21 mm(W) ${\times}$ 26 mm(L), which was identical to the size of the base plate of the CPV. The coolant temperature was controlled by an isothermal bath and the flow rate was controlled and measured by a flowmeter. The experimental results showed that the average of power efficiency of the CPV decreased from 28.6 % to 24.7 % as the cell temperature increased from $36^{\circ}C$ to $97^{\circ}C$. An appropriate cooling method of a CPV might increase the power conversion efficiency by about 4% for the same concentration ratio. Discussion is included from the viewpoint of the combined efficiency in addition to the power efficiency.