• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1085-1089
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• 1999

Energy requirement of Rhode Island Red (RIR) hens was studied by comparative slaughter technique. Seventeen hens above 72 weeks of age were slaughtered in batches. Batch I consisted of 5 hens which were slaughtered initially. Batch II comprised of six hens, which were fed ad libitum broken rice (BR)-based diet for 18 days. Record of feed intake, number of eggs laid and egg weight during the period was kept. These hens were slaughtered and body energy content was determined. Egg energy was consisted as energy deposited. Batch III consisting of six hens which were fed varying quantity of diet for 15 days, were slaughtered similarly as hens of batch II. Regression equation (body weight to body energy) developed on batch I was applied to batch II and developed on batch II was applied to batch III hens, to find out initial body energy content of hens. Egg energy (EE) was calculated according to formula: EE (kcal) = -19.7 + 1.81 egg weight (g). Regressing metabolisable energy (ME) intake on energy balance (body energy change + egg energy), maintenance ME requirement of hens was found to be $119.8kcal/kg\;W^{0.75}/d$. Multiple regression of ME required for production on energy retained as protein and fat (body plus egg energy) indicated that RIR hens synthesize proteins with an efficiency of 85.5 and fat with an efficiency exceeding 100 percent on BR based diet.

• Journal of Nutrition and Health
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• 제18권2호
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• pp.98-114
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• 1985

In the previous studies, one of our author had observed the obligatory nitrogen losses through urine and feces in young Korean male adults, and utilization of whole egg protein in Korean young adults with ordinary intake of energy as a basic study for estimating protein requirements of Korean. This study has tried to determine the protein requirement and utilization when subjects ate the Korean mixed diet at the energy level of 45 kcal per Kg body weight per day. Six healthy Korean young female adults, college students, aged $20{\sim}25$ years old, and $45{\sim}61Kg$ of body weight participated as the study subjects. They were given isocaloric diets with four different protein levels for five days each successively. These diets contained protein levels of 0.45, 0.60, 0.75 and 0.90g per Kg of body weight per day, respectively. It was attempted to observe energy and protein intake, urinary and fecal nitrogen losses, true digestibility of protein, net protein utilization of protein in the body over last two days of period of eating each diet and nitrogen balances were calculated. The body weight change and hematological observation were also performed. The results obtained were summarized as follows ; 1) True digestibility of protein of Korean mixed diets ranged from 83.1% to 86.5% and the average of which was $84.7{\pm}1.7%$. 2) Net protein utilization rate of Korean mixed diets was range of 49% to 55% and the average of which was $52{\pm}3%.$ 3) The body weight of subjects were slightly increased and the values of hemoglobin and hematocrit of the blood were also little increased during the experimental periods of twenty days. 4) Protein requirements of Korean mixed diet with the energy level of 45 kcal/Kg body weight per day of Korean young female adults were estimated as 1.00g/Kg/day on this experimental condition.

• Journal of Nutrition and Health
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• 제44권4호
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• pp.338-343
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• 2011

This study assessed the current EAR, RDA, and AMDR for protein, which were set in 2005 and revised in 2010 as the DRIs for Koreans. A classical approach to establish the EAR for protein has been the nitrogen balance method. This method has practical limitations and problems in statistical analysis by giving over estimations of nitrogen balance. Thus, the present EAR for protein might be lower than the true requirement. Recent reevaluations of nitrogen balance studies by bilinear regression analysis and the IAAO method have indicated that the EAR of 0.66 g/kg bw/d should be increased by 39% to give 0.92 g/kg bw/d. The AMDR for protein in the Korean DRIs was set at 7-10%, which covers almost the entire population's protein intake. Since the 5th percentile of Korean protein intake is close to 10% of energy and due to the beneficial effects of protein beyond the maintenance of nitrogen equilibrium, the lower range of 7% needs to be increased up to 10%. For practical meal arrangement, 15% of energy as protein, which is close to the average protein intake of Koreans, seems to be proper, although the value is almost two times the EAR.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.75-79
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• 1992

Fourteen Sahiwal ${\times}$ Friesian crossbred heifers were used in a 10-wk feeding trial to determine maintenance energy requirements and efficiency of gain. The heifers were individually fed with a diet consisting of 30% dry grass and 70% concentrates at either 110, 140 or 180% of the anticipated maintenance requirement ($494kJ\;ME/kg^{0.75}/day$). Liveweight of individual heifers was measured weekly to calculate diet requirements and average daily gain (ADG). Diet digestibility was determined for all heifers to determine ME intake. Retained energy (RE) of individual heifers was determined from changes in total body fat and protein using a TOH isotope dilution procedure and, assuming calorific values of 39.3 and 23.6 kJ/g for fat and protein respectively. The estimated ME for maintenance was 433 and $470kJ/kg^{0.75}/day$ by liveweight (ADG) equilibrium and energy (RE) equilibrium analysis respectively. ME requirement for one g of liveight gain was 28 kJ.

• 한국가금학회지
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• 제15권3호
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• pp.229-246
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• 1988

본 시험은 하절기 산란계의 생산성 저하 방지 및 사료효율개선을 목적으로 하절기 산란계 사료의 적정 에너지수준 및 단백질수준을 구명하기 위해 대사에너지 3수준(2,500, 2,700, 2,900kcal/kg) 과 단백질 2수준(15%, 17%)을 조합한 6개 처리에 49주령 된 산란기 480수를 공시하여 1988년 6월 10일부터 1988년 9월 1일까지 12주간에 걸쳐 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 생존율은 전처리구에서 98.75~100%로 처리간에 유의성이 없었으며, 대사에너지수준이나 단백질수준간에도 일정한 경향이나 큰 차이가 없었다. 2. 산란율, 난중 및 산란량은 대사에너지 2,500 kcal/kg, 단백질 17%수준에서 각각 84.37%, 62.29g, 그리고 52.55g으로 가장 우수했으며 대사에너지수준이 증가함으로써 점차 낮아졌고, 사료 내 단백질수준이 증가함으로써 유의하게 높아졌으나(p＜0.05), 산란율은 대사에너지 2,500kcal/kg과 2,700kcal/kg, 난중은 2,500kcal/kg과 2,700kcal/kg 및 2,700kcal/kg과 2,900kcal/kg 수준간에는 유의한 차이가 없었다. 3. 사료 섭취량은 대사에너지 2,500kcal/kg, 단백질 55%수준에서 118.28g/day으로 가장 높았으며, 대사에너지수준이 증가함에 따라 사료 섭취량은 유의하게 감소했으나(p＜0.05), 사료중 단백질 수준에 따라서는 큰 차이가 없었다. 4. 대사에너지 섭취량은 대사에너지 2,900kcal/kg, 단백질 15%수준에서 326.60kcal/day로 가장 높았으며 대사에너지수준이 증가함에 따라 유의하게 증가되었으나(p＜0.05), 사료중 단백질 수준에 따라서는 유의한 차이가 없었다. 5. 단백질섭취양은 대사에너지 2,500kcal/kg, 단백질 17%수준에서 19.99 g/day로 가장 높았으며 대사에너지수준이 증가함에 따라 유의하게 감소하였으나, 사료 중 단백질수준이 증가함에 따라 유의하게 증가하였다(p＜0.05). 6 사료 요구율은 대사에너지와 2,500kcal/kg과 단백질 17%수준에서 2.24로 가장 낮았으나, 사료중 대사에너지수준에 따라서는 큰 차이가 없었으나, 단백질수준이 증가함에 따라 유의하게 개선되었다 (p＜0.05). 7. 산란kg당 대사에너지와 단백질 요구량은 2,900kcal/kg와 15% 및 2,700kcal/kg와 17%의 대사에너지와 단백질수준에서 각각 6871.64kcal와 385.76g으로 가장 높았으며 사료중의 에너지수준이 증가할수록 산란kg당 대사에너지요구량은 유의하게 증가하였으나(p＜0.05), 단백노요구량을 일정한 경향을 보이지 않았고. 사료중 단백질수준이 증가함에 따라 산란당 대사에너지 요구량은 유의하게 감소한 반면 산란당 단백질요구량은 유의하게 증가하였다(p＜0.05). 산란kg당 사료비는 2,500kcal/kg과 15%의 대사에너지와 단백질수준에서 279.84 won으로 가장 적었다. 이상의 결과를 종합하여 볼 때, 우리나라 실정에 맞는 하절기 산란계사료의 적정 대사에너지 및 단백질수준은 각각 2,500kcal/kg과 17%가 가장 적합할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.685-692
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• 2003

Information on nutrient requirements and forage intake of fallow weaner deer is required for the development of feeding strategies during the year. An experiment was conducted in which 60 fallow weaner deer (grazing on medic and ryegrass based pastures) were supplemented with a concentrated diet at three levels. The diet contained 2% minerals, 30% lupin and 68% barley grain. Twelve deer from each treatment were dosed with commercial alkane capsules in May, June, July, September and October to predict nutrient intake. The relationships between body weight gain and intake of metabolisable energy and crude protein were established using a general linear models analysis. Dry matter intake from pastures ranged from 0.137 kg to 0.304 kg in May and June and increased to 1.2 kg in October. Nutrient intake from pastures was strongly influenced by amount of supplementary feed and gender. Digestible energy intake from pastures was 1.3, 3.8 and 6.1 MJ/day higher for males than females in July, August and October, respectively. The protein and energy intake was strongly correlated with body weight gain. The energy requirement for maintenance were 7.3, 8.2, 10.2, 10.2 and 10.7 MJ DE/day and the DE required for each kg body weight gain were 19, 18, 29, 34 and 49 MJ in May, June, August and October, respectively. The protein requirement for maintenance was 12.2, 12.6, 15.0, 11.4 and $8.5g/W^{0.75}$ in May, June, July, August and October, respectively. The nutrient requirement defined from this study can be used to assist farmers to explore the possible pasture and stock management practices under southern Australian conditions. However, further research is required to develop rapid and cheap methods for estimating dry matter intake, nutritive value of pastures and to quantify the potential growth rate of fallow deer in southern Australia.

• Journal of Nutrition and Health
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• 제27권4호
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• pp.336-346
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• 1994

balance and to estimate daily energy requirement in 43 Korean female college students maintaining their usual diet and activity level. Energy intake and expenditure were measured in two separate periods about one month apart, each period lasting for 3 days. All the subjects participated in both periods. Energy intake was assessed by two methods ; weighed diet record and duplicate portion analysis of diet minus fecal and urinary excretion. Mean daily energy intake level calculated from diet records was 28.5 kcal/kgB.W(1, 476 kcal/day), and similar to the level of 27.8 kcal/kgB.W(1, 438 kcal/days) obtained from the chemical analysis of duplicate portions. Mean daily energy expenditure, calculated from activity records of each subject, was 34.6 kcal/kgB.W, or 1.39 times BMR, which corresponds to light activity level. Mean daily energy balance of subjects was -5.9 kcal/kgB.W. Mean daily requirement of energy, calculated from the activity records of the subjects, was 34.6 kcal/kgB.W, similar to the level of the Korean RDA for light activity level. The results of this study indicate that 1) the activity level of the study subjects corresponds to the sedentary level ; 2) present Korean RDA for energy for light activity is adequate for the subjects ; and 3) the subjects should increase their energy intake since they are largely in negative energy balance.

• Asian-Australasian Journal of Animal Sciences
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• 제1권1호
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• pp.7-12
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• 1988

Twenty cows, by order of calving, were used in a completely randomized $2{\times}2$ factorial experiment. Variables were tow protein levels (14 and 18% crude protein) and concentration of fat (2 and 6% ether extract) in diets. Fat addition, via unprocessed whole sunflower seed, insured forage utilization in diets to meet energy requirement of cows. A total of 36 wks of lactation was subdivided into three 12-wk stages of lactation. Net energy lactation was set at 1.72, 1.57 and 1.42 Mcal/kg for each stage. Higher protein diets improved the efficiency of energy (FCM/net energy intake) which was particularly noted for diets containing high fat (85.7%). However, diets with low protein-high fat resulted in the lowest efficiency (67.7%). No difference in milk yield and butterfat was due to different levels and combinations of protein and lipid in diets. High protein diets depressed blood cholesterol and glucose compared to low-protein counterparts. Relative decline in milk production was slower for lower fat diets than for higher fat groups, especially mid to later stage of lactation. Results of this experiment tend to support our thesis on the synergistic effect of dietary protein and energy (lipid) upon efficiency of lactation.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1397-1406
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• 2019

Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance ($ME_m$) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs. Methods: Thirty-six growing pigs ($26.3{\pm}1.7kg$) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI ($2,400kJ/kg\;BW^{0.60}\;d$). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein ($RE_p$) and fat ($RE_f$) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment. Results: The average daily gain (ADG) and HP as well as the $RE_p$ and $RE_f$ linearly increased with increasing feed intake (p<0.010). ${\beta}$-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, $kJ/kg\;BW^{0.60}\;d=1.88{\times}ADG$, g/d+782 ($R^2=0.86$) and $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of $RE_p$ and $RE_f$ on MEI. Conclusion: The $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$ in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.107-111
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• 1996

Studies on the effects of protein levels on the total sulphur amino acid (TSAA) requirements of chickens were conducted on the starter broilers by feeding four levels of crude protein(16, 18, 20 and 23%) at three levels of TSAA (0.83, 0.93 and 1.03%) and on the grower broilers by feeding three levels of crude protein (16, 18 and 20%) at three levels of TSAA(0.72, 0.79 and 0.86%). The metabolisable energy of the diets was maintained constant at 3,200 kcal/kg and the experiments were carried out for two growing periods: starter (0-3 wk) and grower (3-6 wk). The results showed that there were significant differences in body weight gain, feed intake and feed:gain ratio under different protein levels of the starters. Crude protein, ME and TSAA intake were significantly affected by increasing the CP levels. The TSAA requirement of the starter broilers is recommended at 0.93% and it is not influenced by different protein levels used in the experiment. For the grower period, body weight gain and feed:gain ratio improved significantly at higher protein diets. Birds fed higher protein diet consumed greater quantities of protein. Responses to TSAA supplementation for body weight gain, feed intake and feed:gain ratio were not significant. The present results showed that the TSAA level of 0.79 to 0.86% was required for grower diets and that the protein levels of the diet did not influence the TSAA requirement.