• The Journal of the Korean Society for Microbiology
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• v.34 no.5
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• pp.453-459
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• 1999

Astrovirus is frequently associated with diarrhea in children. It can not be readily isolated by cell culture, and an electronmicroscope is usually used for detection of this agent. Recently in 1995 a combined method of reverse transcription-polymerase chain reaction (RT-PCR) was designed for easier detection of astrovirus, which is based on the conserved sequence in 3'-end of genomes of the 7 known serotypes of human astrovirus. As of yet there has not been any report of astrovirus data in Korea using the RT-PCR methods. The purpose of this study was to detect astrovirus incidence, severity of symptoms, seasonal variation and co infection rate with rotavirus in Korean children inpatients with diarrhea. Fecal specimens from 61 young children hospitalized with gasteroenteritis Korea from Jan. 1996 through Mar. 1997. They were examined for astroviurs infection by RT-PCR method. Results are as follows:1. Astrovirus was detected at 9.8% (6/61) from fecal specimens of children with severe diarrhea by EIA using monoclonal antibody coated plates. 2. Astorvirus was detected at 29.5% (18/61) from fecal specimens of children with severe diarrhea by RT-PCR. 3. The age of the 18 children affected by astrovirus ranged from 2 monthes to 7 years with mean of 3.0 years. 4. Mean hospital stay of the 18 children was 6.1 days. 5. Five (27.8%) astrovirus RT-PCR positive strains were confirmed in November and in December, respectively out of 18 specimens in total. 6. Astrovirus coinfection with rotavirus type G1 was confirmed in 15/16 specimens (93.8%), and with type G2 was in 1/16 specimens (6.3%).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.11
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• pp.520-526
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• 2019

I-type girders are widely applied as very economical sections in plate girder bridges. There has been research on developing composite laminated panels, curved plates reinforced with closed-end ribs, and new forms of ribs and compression flanges for steel box girders. However, there is a limitation in analyzing the exact cause of local buckling caused by an I-type girder's webs. Therefore, an I-type girder's web was modeled using the finite element analysis program LUSAS 17.0 before and after reinforcement. We checked for the minimum thickness criteria presented in the Korea highway bridge design code, and the cause of buckling after performing a linear elastic buckling analysis of dead and live loads was analyzed. Before reinforcement, an eigenvalue (λ₁) at the 1st mode was 0.7025, the critical buckling load was smaller than the applied load, and there is a buckling. After reinforcement, when applying vertical and horizontal stiffeners to the web part of the girder at support, a Nodal line was formed, the eigenvalue was 1.5272, and buckling stability was secured. To improve buckling trace of the girder at the support, an additional plate was applied to the web at the support to ensure visual and structural safety, but buckling occurs at center of web. The eigenvalue (λ₁) was 3.5299, and this method is efficient for reinforcing the web of the support.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1219-1225
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• 2010

Actinobacillus succinogenes, a representative succinicacid-producing microorganism, is seriously inhibited by ammonium ions, thereby hampering the industrial use of A. succinogenes with ammonium-ion-based materials as the pH controller. Therefore, this study isolated an ammonium-ion-tolerant mutant of A. succinogenes using a continuous-culture technique in which all the environmental factors, besides the stress (ammonium ions), were kept constant. Instead of operating the mutant-generating system as a nutrient-limited chemostat, it was used as a nutrient-unlimited system, allowing the cells to be continuously cultured at the maximum specific growth rate. The mutants were isolated on agar plates containing the acid-base indicator bromothymol blue and a high level of ammonium ions that would normally kill the parent strain by 100%. When cultured in anaerobic bottles with an ammonium ion concentration of 354 mmol/l, the mutant YZ0819 produced 40.21 g/l of succinic acid with a yield of 80.4%, whereas the parent strain NJ113 was unable to grow. When using $NH_4OH$ to buffer the culture pH in a 3.0 l stirredbioreactor, YZ0819 produced 35.15 g/l of succinic acid with a yield of 70.3%, which was 155% higher than that produced by NJ113. In addition, the morphology of YZ0819 changed in the fermentation broth, as the cells were aggregated from the beginning to the end of the fermentation. Therefore, these results indicate that YZ0819 can efficiently produce succinic acid when using $NH_4OH$ as the pH controller, and the formation of aggregates can be useful for transferring the cells from a cultivation medium for various industrial applications.

• Journal of Korean Society of Steel Construction
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• v.27 no.1
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• pp.23-30
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• 2015

Developed in this study were Octagonal-hollow-section(OHS) struts, whose compressive strengths against flexural and local buckling is higher than H-shape or rectangular-hollow-section(RHS) struts with the same unit weight. OHS members are also advantageous in handling and storing compared to circular hollow sections(CHS). OHS members were fabricated from HR Plates by cold forming and fillet welding. 5 numbers of 20m long OHS struts were assembled, each of which consist of two 9.6m long OHS member and two end connection elements made of cast iron. The compressive strength of the OHS strut was evaluated by comparing the test results, design codes and FEM analysis each other. Test results show that all of the struts have almost same or larger compressive strength than Korean Road Bridge Design Code(KRBDC) (2012). The initial imperfections can be estimated by using measured strains and are turned out to be less than L/450 for all the struts tested. The results of FEM analysis show that the variation of initial imperfection has less effects on the compressive strength for struts with vertical surcharge than for those with self-weight only, while the strength decreases as the initial imperfection increases. As the result of this study, the allowable initial imperfection for 20m long OHS struts is recommended to be less than L/350 on job sites.

• Journal of Korean Society of Steel Construction
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• v.23 no.3
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• pp.337-347
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• 2011

In this study, seismic resistance of concrete encased U-shaped steel beam-to-steel H-shaped column connections was evaluated. Three specimens of the beam-to-column connection were tested under cyclic loading. The composite beam was integrated with concrete slab using studs. Re-bars for negative moment were placed in the slab. The primary test parameter was the details of the connections, which are strengthening and weakening strategies for the beam end and the degree of composite action. The depth of the composite beams was 600mm including the slab thickness. The steel beam and the re-bars in the slab were weld-connected to the steel column. For the strengthening strategy, cover plates were weld-connected to the bottom and top flanges of the steel beam. For the weakening strategy, a void using styrofoam box was located inside the core concrete at the potential plastic hinge zone. The test results showed that the fully composite specimens exhibited good strength, deformation, and energy dissipation capacities. The deformation capacity of the beam exceeded 4% rotation angle, which is the requirement for the Special Moment Frame.

• The Journal of the Acoustical Society of Korea
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• v.32 no.2
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• pp.181-189
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• 2013

The purpose of the present study is to investigate the effect of cortical bone on acoustic properties of trabecular bone, such as speed of sound (SOS) and normalized broadband ultrasound attenuation (nBUA), in bovine femur in vitro. Twelve trabecular bone samples and three cortical bone plates with thicknesses of 1.00, 1.47, and 2.00 mm were extracted from the proximal end of two bovine femurs. The correlations between acoustic properties and trabecular apparent bone density were also examined before and after attaching a cortical bone plate to the trabecular bone samples. SOS increased linearly with increasing thickness of the cortical plate attached to one side of ultrasonic incidence of the trabecular bone samples, whereas nBUA showed a nonlinear dependence on the thickness of the cortical plate. All the SOS (r = 0.95-0.97) and nBUA (r = 0.53-0.73) measurements with and without the cortical bone plate with various thicknesses were found to exhibit high correlations with the trabecular apparent bone density. These results imply that the acoustic properties measured in the femur with lateral cortical layers in vitro can be useful indices for the prediction of trabecular bone mineral density.

• Journal of Dental Rehabilitation and Applied Science
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• v.37 no.2
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• pp.61-72
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• 2021

A beautiful smile is made when it is symmetrical and gums are displayed less than 2 - 3 mm. Excessive gingival display also known as "gummy smile" is often recognized to be unaesthetic. Causes of gummy smile can be caused by delayed eruption, vertical maxillary excess, hypermobile upper lip, or a short upper lip. Meanwhile botulinum toxin which is an exotoxin produced from Clostridium botulinum, works by blocking the release of acetylcholine from the cholinergic nerve end plates leading to inactivity of the muscles. The application site and weakens the muscle tone is drawing attention as a gummy smile treatment caused by hypermobile upper lip. There have been many studies about the method of injecting botulinum toxin into muscles around the lips, but there is still no standardized research method and treatment method, so there is controversy over the therapeutic effect. The aim of this study is to review the previous studies about the predictors of indication and effects of gummy smile treatment using botulinum toxin injection. Especially we tried to propose a protocol for optimal dose and efficient injection point through the anatomical considerations for gummy smile treatment using botulinum toxin.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.2
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• pp.25-32
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• 2023

In this study, a double wall system is introduced, which was invented to simplify the complicated manufacturing process of the existing precast concrete (PC) double wall systems and to remove defects such as laitance that may occur during the production of concrete panels. An experimental study was conducted to investigate the tensile resisting capacity of the steel tube which is embedded in the precast concrete panel to keep the spacing between PC panels and to prevent damage of the PC panels during transportation and casting concrete onsite. The experiment was planned to determine the detail of effective steel tube connection considering the steel plate treatment method according to the formation of the opening, the presence of embedded concrete, and the reinforcement welding for additional dowel action as key variables. As a result, the ultimate tensile strength increased by 20-30% compared to the control specimen (ST) except for the steel tube specimen (ST_CP) which has steel plates bent inward at the end part of the steel tube. Since the specimen (ST_CON) filled with concrete inside the control specimen has no additional process and cost for the steel tube connections compared to the control specimen during the production of the developed double wall system, it is determined to be the appropriate detail of steel tube connection.

• Tuberculosis and Respiratory Diseases
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• v.50 no.4
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• pp.426-436
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• 2001

Background : Chronic obstructive pulmonary disease(COPD) is one of the major contributors to morbidity and mortality among the adult population. Cigarette smoking(CS) is undoubtedly the single most important factor in the pathogenesis of COPD. However, its mechanism is unclear. The current hypothesis regarding the pathogenesis of COPD postulates that an imbalance between proteases and antiproteases leads to the destructive changes in the lung parenchyma. This study had two aims. First, to evaluate the effect of CS exposure on histologic changes of the lung parenchyme, and second, to evaluate the effect of CS exposure on the expression of the gelatinolytic enzymes in BAL fluid cells in guinea pigs. Methods : Two groups of five guinea pigs were exposed to the whole smoke of 20 commercial cigarettes per day, 5 hours/day, 5 days/week, for 6weeks, and 12 weeks, respectively, using a smoking apparatus. Five age-matched guinea pigs exposed to room air were used as controls. Five or more sections were microscopically extamined(${\times}400$) and the number of cellular infiltration of the alveolar wall was measured in order to evaluate the effect of CS exposure on the histologic changes of lung parenchyme. The statistical significance was analyzed by a linear regression method. To evaluate the expression of the gelatinolytic enzymes in intraalveolar cells, BAL fluid was obtained and the intraalveolar cells were separated by centrifugation (500 g for 10 min at $4^{\circ}C$). Two sets of culture plates were loaded with $1{\times}10^6$ intraalveolar cells. One plate, contained O.1mM EDTA, a inhibitor of matrix metalloproteases(MMPs), and the other plate had no EDTA. Both plates were incubated for 48 hours at $37^{\circ}C$. After incubation, gelatinolytic protease expression in the supernatants was analyzed by gelatin zymography. Results : At the end of CS exposure, the level of blood carboxy Hb had increased significantly(4.1g/dl in control group, 24g/dl immediately after CS exposure, 18g/dl 30 min after CS exposure, 15g/dl 1 hour after CS exposure). Alveolar inflammatory cells were identified in the CS exposed guinea pigs. The number of alveolar cellular cells observed in a microscopic field ($400{\times}$) was $121.4{\pm}7.2$, $158.0{\pm}20.2$, $196.8{\pm}32.8$, in the control, the 6 weeks, and the 12 weeks group, respectively. The increased extent of inflammatory cellular infiltration of the lung parenchema showed a statistically significant linear relationship with the duration of CS exposure(p=0.001, $r^2=0.675$). Several types of gelatinolytic enzymes in the intraalveolar cells of CS exposed guinea pigs were expressed, of which some were inhibited by EDT A. However, the gelatinolytic enzymes were not expressed in the control groups. Conclusion : CS exposure increases inflammatory cellular infiltration of the alveolar wall and the expression of gelatinolytic proteases in guinea pigs. EDTA inhibits some of the gelatinolytic proteases. These findings suggest a possibility that CS exposure may increase MMP expression in the lungs of guinea pigs.

• The korean journal of orthodontics
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• v.26 no.2 s.55
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• pp.153-162
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• 1996

Rapid maxillary expansion is widely used for the correction of anteroposterior discrepancies, constriction of the maxillary arch, etc. This experiment was undertaken to examine the serial changes in the osteogenesis as well as the collagen fiber bundles in the intermaxillary suture during the rapid maxillary expansion treatment. Four young female dogs aged 6 to 8 months old and not showing menarche yet were used for the experiment. The maxillary impression of dogs were taken, expansion device cast and Hyrax screw soldered at the midline in the 1st premolar area. RME device was delivered to the dogs and the activation of 0.25 mm per quarter-turn was done 2 times per day for 10 days until 5 mm separation was made. Separation of the maxilla was confirmed by X-ray. The animals were sacrificed on 0, 15, 30, 60 days from the finish of maxillary separation and preparations for light microscopy and surface electron microscopy were made. The sutures were cut into frontal serial sections for examination of the histological reactions. The following results were obtained and the conclusions made. 1. The edges of the two palatal plates bordering the midpalatal suture which at the beginning of the retention period were mainly composed of compact bone, underwent extensive resorption followed by new bone formation and gradually became spongy bone rich in bone marrow which in the 60 day retention animal became the compact bone with short intermaxillary suture space. During this transformation, newly formed trabecular bone tissues were added to the original margin. 2. Throughout the expansion period, the collagen fibers underwent successive changes such as stretching, loss of polarity, and finally fibrillogenesis. Towards the end of the expansion procedure, sharpey's fiber formation in newly formed bones were observed. 3. Bony spicules were found in the initial stage of retention on occlusal topographic X-rays, which later were confirmed to have ossified. 4. Judging from the histological changes occuring during the experimental expansion, excessive expansion will cause an excessive bleeding, and retard the remodeling of intermaxillary suture. According to the above results, the bone remodeling after rapid maxillary expansion was preceded by the migration of migratory cells into the intermaxillary suture area. The bone remodeling phenomena were on-going during the 2 months retention sample.