• Journal of Korean Library and Information Science Society
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• v.28
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• pp.93-135
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• 1998

The purpose of this study was to compare the effectiveness of programmed instruction versus lecture and discussion method on the knowledge of basic reference sources among undergraduate library and information science students. The hypotheses of the study were: 1. Programmed instruction will be more effective than the lecture/discussion method with regard to academic achievement. 2. There will be a significant difference in learning time between the experimental and the control groups. Seventy-eight library and information science students were participated m the study from the two universities in Chungchong Province. A programmed instruction manual, including 4-types of reference sources-dictionary, encyclopaedia, bibliography, indexes and abstracts, 40-item multiple choice post-test, and a questionnaire for the students' attitude toward programmed instruction were developed specifically for this research. The post-test only control-group design was selected for this experimental study. Students were given instruction on the specific reference titles in dictionary, encyclopedia, bibliography, indexes and abstracts. The control group was instructed by the lecture and discussion method while the experimental group completed a programmed instruction manual by themselves. Both the control and the experimental group were tested right after the instruction of 4-types of reference sources. In addition, a questionnaire asking students' attitude toward programmed instruction was administered to the experimental group. The findings from this study are summarized as follows: 1. The results showed that there were no significant difference in the mean of the post test score between the two groups. Therefore, programmed instruction is viable as an alternative method of instruction in the teaching of reference sources. 2. There was a significant difference in the mean of time spending for the leaning of bibliography, indexes and abstracts between the two groups. Accordingly, programmed instruction proved to be more efficient than the conventional lecture/discussion method in terms of learning time. 3. Students showed positive response to programmed instruction and evaluated it very interesting and challenging. In conclusion, the programmed instruction method was just as effective as the lecture/discussion method in the teaching of reference sources. And students' attitude toward the programmed instruction was favorable enough to secure a continued use of this method for the teaching of reference sources.

• Journal of the Korean Society for Library and Information Science
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• v.45 no.3
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• pp.107-140
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• 2011

The purpose of this study is to analyze Dongkuksipji's Yeamunji, written by Bae Sang-hyun(裴象鉉) in 1855. Dongkuksipji is a kind of encyclopedia. Dongkuksipji's Yeamunji record 137 books written in Goguryeo, Silla, Goryeo, Joseon and China. Yeamunji has its own classification system which includes 15 different topics. But this classification system emphasizes too much some field, such as Chinese classics of Confucianism. Yeamunji contains information on the period of compilation, author, title, and some bibliographic introductions. Although Dongkuksipji's Yeamunji does not have a perfect system for its contents, it is worthy of notice because it is a large catalogue of ancient books compiled by just one person.

• Molecules and Cells
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• v.38 no.4
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• pp.304-311
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• 2015

Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

• Food Science of Animal Resources
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• v.35 no.5
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• pp.692-702
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• 2015

Lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. To understand the global protein expression profile and metabolic features of L. mucosae LM1 in the early stationary phase, the QExactive^TM Hybrid Quadrupole-Orbitrap Mass Spectrometer was used. Characterization of the intracellular proteome identified 842 proteins, accounting for approximately 35% of the 2,404 protein-coding sequences in the complete genome of L. mucosae LM1. Proteome quantification using QExactive^TM Orbitrap MS detected 19 highly abundant proteins (> 1.0% of the intracellular proteome), including CysK (cysteine synthase, 5.41%) and EF-Tu (elongation factor Tu, 4.91%), which are involved in cell survival against environmental stresses. Metabolic pathway annotation of LM1 proteome using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that half of the proteins expressed are important for basic metabolic and biosynthetic processes, and the other half might be structurally important or involved in basic cellular processes. In addition, glycogen biosynthesis was activated in the early stationary phase, which is important for energy storage and maintenance. The proteogenomic data presented in this study provide a suitable reference to understand the protein expression pattern of lactobacilli in standard conditions

• Genomics & Informatics
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• v.12 no.4
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• pp.181-186
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• 2014

Genome-wide association studies have proven the highly polygenic architecture of complex diseases or traits; therefore, single-locus-based methods are usually unable to detect all involved loci, especially when individual loci exert small effects. Moreover, the majority of associated single-nucleotide polymorphisms resides in non-coding regions, making it difficult to understand their phenotypic contribution. In this work, we studied epistatic interactions associated with three common diseases using Korea Association Resource (KARE) data: type 2 diabetes mellitus (DM), hypertension (HT), and coronary artery disease (CAD). We showed that epistatic single-nucleotide polymorphisms (SNPs) were enriched in enhancers, as well as in DNase I footprints (the Encyclopedia of DNA Elements [ENCODE] Project Consortium 2012), which suggested that the disruption of the regulatory regions where transcription factors bind may be involved in the disease mechanism. Accordingly, to identify the genes affected by the SNPs, we employed whole-genome multiple-cell-type enhancer data which discovered using DNase I profiles and Cap Analysis Gene Expression (CAGE). Assigned genes were significantly enriched in known disease associated gene sets, which were explored based on the literature, suggesting that this approach is useful for detecting relevant affected genes. In our knowledge-based epistatic network, the three diseases share many associated genes and are also closely related with each other through many epistatic interactions. These findings elucidate the genetic basis of the close relationship between DM, HT, and CAD.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.6
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• pp.502-516
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• 2002

Imwonshibyukji is a copying manuscript written around 1825, the 27th year of king Soonjo of the Chosun Period. It is a massive home encyclopedia of total 52 books and 100 volumes. It was quoted from many Chinese literatures The unpublished 'Jeongjoji'of its 17-20th volumes was analyzed to study the cooking methods on the Korean rice cakes (dduck) and studied for the degree of their usefulness and medical values in the light of the cooking and processing science. The book introduced 6 kinds of Korean rice cake: sirudduck (steamed rice cake), chinundduck (steamed and struck rice cake), zizinundduck (small oil fried rice cake), danggui (steamed rice cake mixed with rice power and honey), hondon (=danja: boiled and stuffed rice cake) and butu (fermented rice cake), all of which were composed of total 64 items. The main materials fur the Korean rice cake were as follows: sirudduck and butu were nonwaxy rice powder, chinundduct dinggui and hondon were glutinous rice powder, and zizinundduck was wheat flour. The side material was mainly natural food with the effect of a medicine. The literatures quoted in Imwonshibyukji are as follows: 4 Korean books including Ongheejabji were quoted total 28 times (41.5%), 5 Chinese books including Junsengpaljeon were quoted total 35times (52.2%), and I Japanese book Whawhansamjedohoi was quoted total 4 times (6.0%). The Korean rice cakes quoted in the Korean literature so far exist but the Chinese ones no longer exist. In the comparative analysis of Imwonshibyukji and other contemporary literatures, Chosunmoossangsinsikyorijebub was the most similar one to the book among others.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.343-351
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• 2018

Background: Red ginseng is a popularly used traditional medicine with antiaging effects in Asian countries. The present study aimed to explore the changes in protein expression underlying the mechanisms of life span extension and antiaging caused by red ginseng extract (RGE) in Drosophila melanogaster. Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods. Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster. Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.

• Journal of architectural history
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• v.16 no.1
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• pp.29-48
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• 2007

This study is an investigation that explication of the reading method of transcription marking, and explains what it means and where to use transcription marking of architectural vocabulary. focusing on timber class vocabulary from on SeoGwolYeongGeonDoGamUiGwe(${\ulcorner}$西闕營建都監儀軌(1831)${\lrcorner}$). This study is reviewed sequentially classifying items according to timber class, stone class, iron class architectural vocabulary. This treatise, named 'Architectural Vocabulary Study (1)', is mainly focused on making out a transcription marking of timber class vocabulary on the documents. But, because of the properties of the UiGwe(儀軌), there are some different expressions used for the same vocabulary. Therefore, the different expressions are referenced according to vocabulary used from the 17th century to the 20c century. This study lists timber class architectural vocabulary of transcription marking on SeoGwolYeongGeonDoGamUiGwe(${\ulcorner}$西闕營建都監儀軌(1831)$\lrcorner$). This study also explicates and explains the meaning of them. In advance, this study compares them with the vocabulary of national language in the middle ages and modern times. If we could explicate the transcription marking of the documents as it is, we can't only recover plenty of vocabulary related with characteristic architecture in the age of later Chosun Dynasty, but also correct wrongly used vocabulary nowadays. With the results, we can standardize and adjust vocabulary use of Korean traditional architecture. In advance, we can correct errors of spelling and mistaken explanation in the Korean Encyclopedia.

• Journal of Ginseng Research
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• v.44 no.2
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• pp.312-320
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• 2020

Background: Ginseng (Panax ginseng Meyer) is an essential source of pharmaceuticals and functional foods. Ginseng productivity has been compromised by high light (HL) stress, which is one of the major abiotic stresses during the ginseng cultivation period. The genetic improvement for HL tolerance in ginseng could be facilitated by analyzing its genetic and molecular characteristics associated with HL stress. Methods: Genome-wide analysis of gene expression was performed under HL and recovery conditions in 1-year-old Korean ginseng (P. ginseng cv. Chunpoong) using the Illumina HiSeq platform. After de novo assembly of transcripts, we performed expression profiling and identified differentially expressed genes (DEGs). Furthermore, putative functions of identified DEGs were explored using Gene Ontology terms and Kyoto Encyclopedia of Genes and Genome pathway enrichment analysis. Results: A total of 438 highly expressed DEGs in response to HL stress were identified and selected from 29,184 representative transcripts. Among the DEGs, 326 and 114 transcripts were upregulated and downregulated, respectively. Based on the functional analysis, most upregulated and a significant number of downregulated transcripts were related to stress responses and cellular metabolic processes, respectively. Conclusion: Transcriptome profiling could be a strategy to comprehensively elucidate the genetic and molecular mechanisms of HL tolerance and susceptibility. This study would provide a foundation for developing breeding and metabolic engineering strategies to improve the environmental stress tolerance of ginseng.

• Journal of the Korean association of regional geographers
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• v.19 no.1
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• pp.14-30
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• 2013

Place names in North Korea has been regarded as an effective instrument of revolution since division of territory(1945) and as typical case which politcal ideology affected the place names. Especially in North Korea, self-reliance ideology(Juche Idea) and idolization of Kim Il Sung influenced the place names. With local administrative district reform in 1952, names of district and village were changed on national scale. National survey of place names were proceeded in 1964~1966 with direct support of Kim, Ilsung. After this survey, North Korea made alteration of place names in terms of idolization of Kim Il Sung family as well as socialist revolution. Encyclopedia of place names were widely published. Almost linguist were forced to produce writing and papers for the praise of the legitimacy of new place names. But it should be attended that research trend are slowly changed since 2000s. Research for idolization of Kim Il Sung has become a little importance.