• The Journal of Korean Medicine
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• v.19 no.1
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• pp.19-26
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• 1998

The purpose of present study is to clarify the differences between EIslwitzia Ciliata (Thunb.) Hylander(향유) and Elsholtzia splendens Nakai ex F. Maekawa (꽃향유) for standardization and the proper usage as medicinal herbs. The major ingredients of both species were isolated by distillation and extraction. The qualitative and quantitative analyses of major distillates were carried out by the use of GC/MS. There was a significant difference between the components of Elsholtzia ciliata and Elsholtzia splendens in the aspects of major components. Several common ingredients were identified as linalool, cumene, elsholtzia ketone, naginata ketone isomer, naginata ketone, myristicin, and sesquiterpene alcohol. Comparison between Elsholtzia. ciliata and Elsholtzia splendens was done in the aspect of major compounds. Myristicin (33.7%) has been shown to be the major component in Elsholtzia ciliata whereas naginata ketone isomer (26.1%) was believed to be a major ingredient in Elsholtzia splendens. The elsholtzia ketone was also one of the major differentiating factors between Elsholtzia splendens and Elsholtzia ciliata, and the quantity is 15.1% in Elslwltzia splendens compared to 2.87% in Elsholtzia ciliata. Moreover, in the Elsholtzia splendens, 4- vinylguaiacol and isoosmorhizole were absent, but both compounds were present in the EIsholtzia ciliata.

• Food Science and Preservation
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• v.13 no.3
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• pp.363-368
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• 2006

This study was performed to develop flavor materials from Elsholtzia ciliata Hylander with analyzing functionality and aroma profile and to optimize supercritical fluid extraction method and optimum condition. The qualities of water extracts such as total yield total phenolic compound electron donation ability, estragole and L-carvone, were affected by extraction pressure than time. The response variables had significant with pressure than with time and the established polynomial model was suitable(P>0.05) model by Lack-of-Fit analysis. The optimum extraction conditions which were limited of maximum value for dependent variables under experimental conditions based on central composite design were 238 bar and 42 min.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.463-472
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• 2004

This study was carried out to investigate the chemical composition of essential oils, absolutes and oleoresins isolated from Elsholtzia ciliata and the biological activities of them. Yields of essential oils, absolutes and oleoresins were 0.34%, 11.33% and 15.24%, respectively. The major component was naginate ketone in essential oils, methyl linolenate in absolutes and 9,12,15-octadecatrienoic acid in oleoresins. Eseential oils and oleoresins showed the inhibitory activities in enzyme-dependent, enzyme-independent and autooxidatve lipid peroxidation systems. $EC_{50}$ values in neutral red uptake assays 24 h of exposure times were $23.3\;{\mu}g/m{\ell}$, $341.0\;{\mu}g/m{\ell}$ and $17.2\;{\mu}g/m{\ell}$ in essential oils, absolutes and oleoresins, respectively, and essential oils and oleoresins showed the cytotoxic effect at the only high dose. Absolutes and oleoresins did not show antibiotic and mutagenic activities. On the contrary, essential oils with over $500\;{\mu}g/plate$ showed antibiotic and mutagenic activities in Ames test. Essential oils and oleoresins have a prolongating effect the ciliostasis of rat trachea.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.6
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• pp.341-347
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• 2020

The purpose of this study was to investigate the possibility of herbal medicine complex extract (NI-01), which were prepared from 6 natural materials (Cinnamomum cassia Blume, Lonicerae Flos, Paeonia suffruticosa Andrews, Arctium lappa Linne, Schzandra chinesis Bailon, Elsholtzia ciliata Hylander), as a functional material for inhibition of atopic dermatitis. anti-oxidative activity was confirmed by measuring DPPH electron donating ability and ABTS+ radical scavenging ability. Cytotoxicity and NO inhibition were measured using RAW 264.7 cells to confirm anti-inflammatory efficacy. The test substance was orally administered to the pruritus-induced ICR mice to confirm the inhibition of pruritus. The bovine cornea opacity and permeability (BCOP) assay was performed to confirm safety for irritation. NI-01 showed high antioxidant activity in DPPH and ABTS+ methods. In the anti-inflammatory effect tests with RAW 264.7 cells, NO production was inhibited at NI-01 concentrations of 50 (14.9%) and 100 (4.2%) ㎍/mL, which indicated that the anti-inflammatory effect was increased in a concentration-dependent manner. NI-01 also showed anti-itching effect after inducing of itching by compound 48/80 in ICR mice. NI-01 was proved to be a non-irritant substance in BCOP assay. The results of this study suggested that the herbal medicine combined extract (NI-01) has high antioxidant, anti-inflammatory and anti-itching effects, and safety for irritation. Therefore, herbal medicine complex extract (NI-01) is thought to be highly applicable for the inhibitory ingredients of the atopic dermatitis.