• Journal of Plant Biotechnology
• /
• v.39 no.4
• /
• pp.225-234
• /
• 2012

This report describes recent advances in tissue culture and genetic transformation of commercial Phalaenopsis. Recently, an importance of Phalaenopsis has been increased due to its popularity with beautiful flowers and is widely used for pot plants as well as cut-flower. Its use is rapidly enlarging in worldwide. Thus, demands for the release of new elite cultivars in Phalaenopsis have been increased. During the last several decades, some critical progresses have been made in tissue culture and genetic transformation in Phalaenopsis species. Cooperation with these biotechnological methods are supposed to promote the release of commercial Phalaenopsis cultivars in the near future. Until now, no technical review on tissue culture and genetic transformation in Phalaenopsis has been reported in Korea. Therefore, we inquired the brief history and techniques of tissue culture system in Korea.

• Smart Structures and Systems
• /
• v.22 no.4
• /
• pp.459-468
• /
• 2018

In this paper, the effect of non-persistent joints was determined on the behavior of concrete specimens subjected to biaxial loading through numerical modeling using particle flow code in two dimensions (PFC2D). Firstly, a numerical model was calibrated by uniaxial, Brazilian and triaxial experimental results to ensure the conformity of the simulated numerical model's response. Secondly, sixteen rectangular models with dimension of 100 mm by 100 mm were developed. Each model contains two non-persistent joints with lengths of 40 mm and 20 mm, respectively. The angularity of the larger joint changes from $30^{\circ}$ to $90^{\circ}$. In each configuration, the small joint angularity changes from $0^{\circ}$ to $90^{\circ}$ in $30^{\circ}$ increments. All of the models were under confining stress of 1 MPa. By using of the biaxial test configuration, the failure process was visually observed. Discrete element simulations demonstrated that macro shear fractures in models are because of microscopic tensile breakage of a large number of bonded discs. The failure pattern in Rock Bridge is mostly affected by joint overlapping whereas the biaxial strength is closely related to the failure pattern.

• Computers and Concrete
• /
• v.20 no.4
• /
• pp.429-437
• /
• 2017

In this study, the effect of the tensile strength and ratio of disc spacing to penetration depth on the efficiency of tunnel boring machine (TBM) is investigated using Particle flow code (PFC) in two dimensions. Models with dimensions of $150{\times}70mm$ made of rocks with four different tensile strength values of 5 MPa, 10 MPa, 15 MPa and 20 MPa were separately analyzed and two "U" shape cutters with width of 10 mm were penetrated into the rock model by velocity rate of 0.1 mm/s. The spacing between cutters was also varied in this study. Failure patterns for 5 different penetration depths of 3 mm, 4 mm, 5 mm, 6 mm, and 7 mm were registered. Totally 100 indentation test were performed to study the optimal tool-rock interaction. An equation relating mechanical rock properties with geometric characteristics for the optimal TBM performance is proposed. The results of numerical simulations show that the effective rock-cutting condition corresponding to the minimum specific energy can be estimated by an optimized disc spacing to penetration depth, which, in fact, is found to be proportional to the rock's tensile strength.

• Structural Engineering and Mechanics
• /
• v.68 no.5
• /
• pp.537-547
• /
• 2018

This research presents the effect of anisotropy of the hollow disc mode under Brazilian test using PFC3D. The Brazilian tensile strength test was performed on the hollow disc specimens containing the bedding layers and then these specimens were numerically modeled by using the two dimensional discrete element code (PFC3D) to calibrate this computer code for the simulation of the cracks propagation and cracks coalescence in the anisotropic bedded rocks. The thickness of each layer within the specimens varied as 5 mm, 10 mm and 20 mm and the layers angles were changed as $0^{\circ}$, $25^{\circ}$, $50^{\circ}$, $75^{\circ}$ and $90^{\circ}$. The diameter of internal hole was taken as 15 mm and the loading rate during the testing process kept as 0.016 mm/s. It has been shown that for layers angles below $25^{\circ}$ the tensile cracks produce in between the layers and extend toward the model boundary till interact and break the specimen. The failure process of the specimen may enhance as the layer angle increases so that the Brazilian tensile strength reaches to its minimum value when the bedding layers is between $50^{\circ}$ and $75^{\circ}$ but its value reaches to maximum at a layer angle of $90^{\circ}$. The number of tensile cracks decreases as the layers thickness increases and with increasing the layers angle, less layer mobilize in the failure process.

• Structural Engineering and Mechanics
• /
• v.69 no.1
• /
• pp.51-67
• /
• 2019

In this paper, the anisotropy of tensile behaviours of layered rocks consisting internal notch has been investigated using particle flow code. For this purpose, firstly calibration of PFC2D was performed using Brazilian tensile strength. Secondly Brazilian test models consisting bedding layer was simulated numerically. Thickness of layers was 10 mm and layered angularity was $90^{\circ}$, $75^{\circ}$, $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. The strength of bedding interface was too high. Each model was consisted of one internal notch. Notch length is 1 cm, 2 cm and 4 cm and notch angularities are $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. Totally, 90 model were tested. The results show that failure pattern was affected by notch orientation and notch length. It's to be noted that layer angle has not any effect on the failure pattern. Also, Brazilian tensile strength is affected by notch orientation and notch length.

• Structural Engineering and Mechanics
• /
• v.66 no.5
• /
• pp.569-582
• /
• 2018

Effects of crack separation, bridge area, on the tensile behaviour of concrete are studied experimentally and numerically through the Brazilian tensile test. The physical data obtained from the Brazilian tests are used to calibrate the two-dimensional particle flow code based on discrete element method (DEM). Then some specially designed Brazilian disc specimens containing two parallel cracks are used to perform the physical tests in the laboratory and numerically simulated to make the suitable numerical models to be tested. The experimental and numerical results of the Brazilian disc specimens are compared to conclude the validity and applicability of these models used in this research. Validation of the simulated models can be easily checked with the results of Brazilian tests performed on non-persistent cracked physical models. The Brazilian discs used in this work have a diameter of 54 mm and contain two parallel centred cracks ($90^{\circ}$ to the horizontal) loaded indirectly under the compressive line loading. The lengths of cracks are considered as; 10 mm, 20 mm, 30 mm and 40 mm, respectively. The visually observed failure process gained through numerical Brazilian tests are found to be very similar to those obtained through the experimental tests. The fracture patterns demonstrated by DEM simulations are mostly affected by the crack separation but the tensile strength of bridge area is related to the fracture pattern and failure mechanism of the testing samples. It has also been shown that when the crack lengths are less than 30 mm, the tensile cracks may initiate from the cracks tips and propagate parallel to loading direction till coalesce with the other cracks tips while when the cracks lengths are more than 30 mm, these tensile cracks may propagate through the intact concrete itself rather than that of the bridge area.

• Structural Engineering and Mechanics
• /
• v.60 no.6
• /
• pp.939-952
• /
• 2016

A compression to tensile load transforming (CTT) device was developed to determine indirect tensile strength of concrete material. Before CTT test, Particle flow code was used for the determination of the standard dimension of physical samples. Four numerical models with different dimensions were made and were subjected to tensile loading. The geometry of the model with ideal failure pattern was selected for physical sample preparation. A concrete slab with dimensions of $15{\times}19{\times}6cm$ and a hole at its center was prepared and subjected to tensile loading using this special loading device. The ratio of hole diameter to sample width was 0.5. The samples were made from a mixture of water, fine sand and cement with a ratio of 1-0.5-1, respectively. A 30-ton hydraulic jack with a load cell applied compressive loading to CTT with the compressive pressure rate of 0.02 MPa per second. The compressive loading was converted to tensile stress on the sample because of the overall test design. A numerical modeling was also done to analyze the effect of the hole diameter on stress concentrations of the hole side along its horizontal axis to provide a suitable criterion for determining the real tensile strength of concrete. Concurrent with indirect tensile test, the Brazilian test was performed to compare the results from two methods and also to perform numerical calibration. The numerical modeling shows that the models have tensile failure in the sides of the hole along the horizontal axis before any failure under shear loading. Also the stress concentration at the edge of the hole was 1.4 times more than the applied stress registered by the machine. Experimental Results showed that, the indirect tensile strength was clearly lower than the Brazilian test strength.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.17 no.2
• /
• pp.391-411
• /
• 2023

Aggregates play the skeleton and supporting role in the construction field, high-precision measurement and high-efficiency analysis of aggregates are frequently employed to evaluate the project quality. Aiming at the unbalanced operation time and segmentation accuracy for multi-class segmentation algorithms of aggregate images, a Chaotic Sparrow Search Algorithm (CSSA) is put forward to optimize it. In this algorithm, the chaotic map is combined with the sinusoidal dynamic weight and the elite mutation strategies; and it is firstly proposed to promote the SSA's optimization accuracy and stability without reducing the SSA's speed. The CSSA is utilized to optimize the popular multi-class segmentation algorithm-Multiple Entropy Thresholding (MET). By taking three METs as objective functions, i.e., Kapur Entropy, Minimum-cross Entropy and Renyi Entropy, the CSSA is implemented to quickly and automatically calculate the extreme value of the function and get the corresponding correct thresholds. The image adaptive multi-class segmentation model is called CSSA-MET. In order to comprehensively evaluate it, a new parameter I based on the segmentation accuracy and processing speed is constructed. The results reveal that the CSSA outperforms the other seven methods of optimization performance, as well as the quality evaluation of aggregate images segmented by the CSSA-MET, and the speed and accuracy are balanced. In particular, the highest I value can be obtained when the CSSA is applied to optimize the Renyi Entropy, which indicates that this combination is more suitable for segmenting the aggregate images.

• Smart Structures and Systems
• /
• v.25 no.4
• /
• pp.487-499
• /
• 2020

This paper proposes a novel approach to model updating for a large-scale cable-stayed bridge based on ambient vibration tests coupled with a hybrid metaheuristic search algorithm. Vibration measurements are carried out under excitation sources of passing vehicles and wind. Based on the measured structural dynamic characteristics, a finite element (FE) model is updated. For long-span bridges, ambient vibration test (AVT) is the most effective vibration testing technique because ambient excitation is freely available, whereas a forced vibration test (FVT) requires considerable efforts to install actuators such as shakers to produce measurable responses. Particle swarm optimization (PSO) is a famous metaheuristic algorithm applied successfully in numerous fields over the last decades. However, PSO has big drawbacks that may decrease its efficiency in tackling the optimization problems. A possible drawback of PSO is premature convergence leading to low convergence level, particularly in complicated multi-peak search issues. On the other hand, PSO not only depends crucially on the quality of initial populations, but also it is impossible to improve the quality of new generations. If the positions of initial particles are far from the global best, it may be difficult to seek the best solution. To overcome the drawbacks of PSO, we propose a hybrid algorithm combining GA with an improved PSO (HGAIPSO). Two striking characteristics of HGAIPSO are briefly described as follows: (1) because of possessing crossover and mutation operators, GA is applied to generate the initial elite populations and (2) those populations are then employed to seek the best solution based on the global search capacity of IPSO that can tackle the problem of premature convergence of PSO. The results show that HGAIPSO not only identifies uncertain parameters of the considered bridge accurately, but also outperforms than PSO, improved PSO (IPSO), and a combination of GA and PSO (HGAPSO) in terms of convergence level and accuracy.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.10-10
• /
• 2017

Rice consumption has been continuously decreasing as the eating habits of Koreans have become westernized and diversified. The per capita annual rice consumption in Korea has dropped sharply from 136.4 kg in 1970 to 61.9 kg in 2016. The Korean government, therefore, has been trying to promote rice consumption by invigorating the processed food industry using rice flour. To facilitate the market for processed rice foods, it is essential to develop proper milling technology in terms of flour particle size and damaged starch content to produce high quality rice flour at competitive cost. Dry milling and wet milling are the two major processes used to produce rice flour. Although the dry milling process is relatively simple with a lower production cost, damaged starch content increases because of the high grain hardness of rice. In wet milling, the quality of rice flour is improved by reducing flour particle size as well as damaged starch content through soaking procedures. However, the production costs are high because of the additional expenses associated with the disposal of waste water, sterilization and drying of the wet flour. Recently developed technologies such as jet milling and cryogenic milling also require expensive investment and production. Therefore, developing new rice cultivars with dry milling adaptability as well as good processing properties is an important goal of rice breeding in Korea. 'Suweon 542' is a floury endosperm mutant line derived from sodium azide treatment on a high-yield, early maturing, and non-glutinous japonica rice cultivar, 'Namil'. Compared with the wild type, after dry milling process, the grain hardness of 'Suweon 542' was significantly lower because of its round and loosely packed starch granules. Also, the flour of 'Suweon 542' had significantly smaller particles and less damaged starch than 'Namil' and other rice cultivars and its particle size distribution was similar to a commercial wheat cultivar. Recently, through collaborations with nine universities and food companies, a total of 21 kinds of processed prototypes, using the dry milling flour of 'Suweon 542', were evaluated. In the production of major rice processing products, there was no significant quality difference between the flours prepared by wet milling and dry milling. Although the amount of water added to the dough was slightly increased, it was confirmed that the recipe applying the wet flour could be used without significant change. To efficiently transfer the floury endosperm characteristics of 'Suweon 542' to other commercial rice cultivars, it is essential to develop DNA marker tightly linked to the target gene. Association analysis using 70 genome-wide SSR markers and 94 F2 plants derived from 'Suweon 542'/'Milyang 23' showed that markers on chromosome 5 explained a large portion of the variation in floury grains percentage (FGP). Further analysis with an increased number of SSR markers revealed that the floury endosperm of 'Suweon 542' was directed by a major recessive locus, flo7(t), located in the 19.33-19.86 Mbp region of chromosome 5, with RM18639 explaining 92.2% of FGP variation in the F2 population. Through further physical mapping, a co-segregate and co-dominant DNA marker with the locus, flo7(t) was successfully developed, by which, thereby, breeding efficiency of rice cultivars having proper dry milling adaptability with high yield potential or useful functional materials would be improved. 'Suweon 542' maintained the early maturity of the wild type, Namil, which can be used in rice-wheat double cropping systems in Korea not only for improved arable land but also for sharing flour production facilities. In addition to the high susceptibility against major rice diseases, nevertheless, another possible drawback of 'Suweon 542' is the high rate of viviparous under prolonged rainfall during the harvesting season. To overcome susceptibility and vivipary of 'Suweon 542', the progeny lines, derived from the crosses 'Suweon 542' and 'Jopyeong', an early maturing rice cultivar with multiple resistance against rice blast, bacterial blight, and rice strip virus, and 'Heugjinju', a anthocyanin pigment containing black rice cultivar, were intensively evaluated. As the outputs, three dry milling suitable rice elite lines, 'Jeonju614', 'Jeonju615', and 'Jeonju616' were developed.