• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.82-90
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• 2016

Most Araliaceae plant species distributed in Korea are economically important because of their high medicinal values. This study was conducted to develop barcode markers from sequence analysis of chloroplast DNA in 14 taxa of Araliaceae species grown in South Korea. Sequencing of seven chloroplast DNA regions was performed to establish the DNA barcode markers, as suggested by the Consortium for the Barcode of Life (CBOL). From the sequence analysis of chloroplast DNA, we identified specific sequences and nucleotides that allowed us to discriminate among each other 14 Korean Araliaceae species. The sequence in the region of psbA-trnH revealed the most frequent DNA indels and substitutions of all 7 regions studied. This psbA-trnH marker alone can discriminate among all 14 species. There are no differences between Korean and Chinese Panax ginseng in all seven sequenced chloroplast DNA regions. A phylogenetic tree constructed using the seven chloroplast DNA regions revealed that Tetrapanax papyriferus should be classified as an independent clade. The Aralia and Panax genera showed a close phylogenetic relationship. Five species in the Eleutherococcus genus were more closely related to Kalopanax septemlobus than to any Panax species.

• The Korea Journal of Herbology
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• v.25 no.1
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• pp.75-81
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• 2010

Objectives : It was investigated new herbal prescription HT005 supported by traditional Korean medicine has a activity on the longitudinal bone growth of rats. HT005 were consisted of the root of Angelica sinensis, the fruit of Alpinia oxyphylla, the root of Astragalus membranaceus, the stem of Eleutherococcus senticosus, and the root of Dioscorea japonica, and Poria cocos. Method : To investigate the effect on longitudinal bone growth in adolescent male Sprague-Dawley rats, the longitudinal length of growth plate was measured directly by the tetracycline fluorescent labeling method and chondrocyte staining method. HT005 administered orally for four days, and the tetracycline was injected twice on the growth plate of the animals for fluorescent dying. The rate of longitudinal bone growth was measured the length between the tetracycline bands which fixed on the 3rd day and 5th day after injection. Cresyl violet was also used to stain the chondrocytes in the growth plate. The length of growth plate after administration was compared. Expression of IGF-1 and BMP-2 in the growth plate was investigated by immunohistochemistry. Results : HT005 showed a significant longitudinal bone growth which was $301.0{\pm}6.1\;{\mu}m/day$ at the dose of 100 mg/kg and $283.8{\pm}1.25\;{\mu}m/day$ (p < 0.001)at the dose of 10 mg/kg of HT005, compared to control group by the tetracycline fluorescent labeling method. HT005 showed a significant chondrocyte length on growth plate which was measured $797.19{\pm}3.31\;{\mu}m$ (p < 0.001) at the dose of 100 mg/kg and $720.14{\pm}2.19$ (p < 0.001) ${\mu}m$ at the dose of 10 mg/kg compared to the control group by cresyl-violet staining method. Both the number and intensity of BMP-2 and IGF-1 positive cells were increased in the hypertrophic zone of growth plate. There was a significant correlation between BMP-2 and IGF-1 expression and heights of chondrocytes in growth plate. Conclusions : Treatment of HT005 on Sprague-Dawley rats markedly increased the longitudinal bone growth. Therefore, HT005 may be an alternative herbal source to growth hormone as it promotes bone growth in children afflicted by growth retardation.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.293-308
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• 2014

This study was carried out to investigate the vascular plants in the construction site of the National DMZ Native Botanic Garden. The period of survey was from May 2012 to November 2013. Vascular plants based on voucher specimen were summarized as 313 taxa including 79 families, 211 genera, 272 species, 4 subspecies, 32 varieties, 4 forms and 1 hybrids. The rare plant species designated by Korea Forest Service were 8 taxa including Galium boreale L., Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., Eranthis stellata Maxim. and Lloydia triflora (Ledeb.) Baker, etc. Endemic plant species were 4 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, Philadelphus schrenkii Rupr. and Cirsium setidens (Dunn) Nakai. Furthermore, 51 taxa were listed as specific plant species based on phytogeographical in the investigated area. The naturalized plants were recorded as 11 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 3.51%, and 3.43%, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.5-5
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• 2010

제주지역에 분포하는 특산식물의 현황을 알아보기 위해 지난 2007년부터 2010년까지 현지조사를 실시하여 44과 75속 63종 17변종 13품종으로 총 93분류군에 대한 자생지 환경, 개체군 동태, 생육특성 및 위협요인 등에 대한 자료를 구축하였다. 이중 세계적으로 제주지역에만 분포하는 제주특산식물은 긴다람쥐꼬리(Huperzi integrifolium) 등 27과 46속 36종 12변종 8품종으로 총 56분류군으로 분석되었으며, 제주도를 포함하여 우리나라에만 분포하는 한국특산식물은 구상나무(Abies koreana) 등 20과 28속 24종 4변종 4품종으로 총 32분류군인 것으로 파악되었다. 그리고 조사대상 특산식물 중 바위좀고사리(Asplenium sarelii var. anogrammoides) 등 5분류군은 분류학적 검토와 함께 특산식물여부에 대한 검토가 필요한 것으로 판단되었다. 그리고 개나리(Forsythia koreana)는 조경용으로 식재된 종이므로 제주지역에 자생지가 확인된 특산식물은 39과 70속 59종 16변종 12품종으로 총 87분류군으로 볼 수 있다. 제주지역의 특산식물은 해안지대에서 확인된 갯겨이삭(Puccinellia coreensis)부터 한라산 정상에서 자라는 한라솜다리(Leontopodium hallaisanense)까지 제주도 전역 분포하고 있으나 종에 따라 서로 다른 분포특성을 보였다. 특히, 한라산 해발 1,400m 이상 아고산대에 집중 분포하는 특성을 보였다. 특산식물의 자생지내 개체군의 동태를 보면 대부분의 식물이 개체수 및 분포범위가 한정되어 있는 것으로 나타났다. 더욱이 지리산오갈피(Eleutherococcus divaricatus var. chiisanensis)는 2개체만이 확인되어 종보전이 시급한 것으로 조사되었고, 한라솜다리, 모데미풀(Megaleranthis saniculifolia), 한라각시둥굴레(Polygonatum humile var. humillimum) 등 상당수의 식물 종이 수십 개체 이내로 매우 적은 개체수를 유지하고 있었다. 제주지역에 분포하는 특산식물은 인위적 또는 자연적 요인으로 인해 많은 자생지 면적감소나 개체수 감소 등의 위협요인을 갖고 있는 것으로 나타났다. 특히, 중산간 이하에 자라는 특산식물은 접근성 등이 용이하여 도채 등의 위험성이 높은 것으로 판단된다. 그리고 한라산국립공원 내에 분포하는 특산식물은 인위적 훼손 위험성은 상대적으로 낮은 편이나 자연적 요인 즉, 식생변화 등으로 인해 자생지 및 개체수 감소가 이루어질 것으로 보여 체계적인 종 보존전략이 마련되어야 할 것으로 판단된다. 한편, 제주지역 특산식물 중 자생지가 확인된 식물 종을 대상으로 근연종 분석 등을 통해 유용성을 분석하면 식물체 일부가 약용으로 가능한 식물 종이 개족도리(Asarum maculatum) 등 전체의 67.1%를 차지하였고, 관상용으로 활용가치가 높은 식물 종은 구상나무(Abies koreana) 등 전체의 60.2%인 것으로 분석되었으나 대부분의 종이 희소성의 가치가 있어 모든 특산식물이 관상용으로 이용가치가 있는 것으로 판단되었다. 제주지역에서 확인된 특산식물 중 전체적으로 90.9%가 유용성이 있는 것으로 나타나고 있으며 아직까지 유용성에 대한 정보가 미흡한 종도 자원적인 연구접근에 따라 다양한 자원으로 활용가치가 있는 것으로 판단된다.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.447-451
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• 2012

In order to investigate the efficacies for cognitive function of edible plants, we measured the inhibitory effects of acetylcholinesterase(AChE) activity, radical scavenging activities, and the contents of GABA and glutamate in the plant extracts. Among the plant extracts, Schizandra chinensis contained the highest GABA 14.8 mg/g and the extracts of Cnidium officinale and Polygonum multiflorum also had a relatively high GABA. On the other hand, plant extracts except, Acorus gramineus, showed similar glutamate contents. S. chinensis, Hovenia dulcis, Thuja orientalis, and Eleutherococcus senticosus exhibited high inhibition against AChE activities at about 18-33% at 1 mg/mL. In addition, strong radical scavenging activities were also detected in those extracts which showed high AChE inhibition. Taken together, H. dulcis, T. orientalis, E. senticosus, and S. chinensis could be effective resources for enhancing cognitive function. Further, it was suggested that the AChE inhibitory activities of plant extracts might be related to antioxidative activity.

• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.1-19
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• 2002

The use of herbals has increased considerably while their efficacy and safety remain untested. This unsupported surge in demand has prompted a call for their clinical evaluation. One area in which evaluations are emerging is ginseng and diabetes. Growing evidence is accumulating from in vitro and animal models indicating that various ginseng species, American (Panax quinquefolius L), Asian (Panax ginseng C.A. Meyer), Korean Red, San-chi (Panax notoginseng [Burk.] P.R. Chen), and the non-panax species Siberian (Eleutherococcus senticossus) ginsing, and their fractions, saponins (ginsenosides) and peptidoglycans (panaxans for panax species and eleutehrans for Siberian ginseng), might affect carbohydrate metabolism and related signaling molecules. Recent human studies from our laboratory have also shown a blood glucose lowering effect of American ginseng (AG) and some other ginseng spices postprandially after acute administration and chronically after administration for 8-weeks in people with type 2 diabetes. Although generally encouraging, these data only indicate a need for more evaluations of ginsengs safety and efficacy. Because of poor industry standardization, it is not known whether all ginsengs will affect blood glucose. In this regards some ginseng batches have demonstrated null effects while others have even raised postprandial glycemia. Clinical research should therefore focus on components involved in its glucose lowering effects.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.379-406
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• 2019

A floristic study on the vascular plants from Juwangsan National Park was conducted 13 times in total, from March to October 2017. Based on the voucher specimens and pictures, it was revealed that the vascular plants in this region comprised 758 taxa: 112 families, 399 genera, 672 species, 4 subspecies, 72 varieties, and 10 forma. Among these plants, 221 taxa were recorded for the first time from this region. The taxa in category II of endangered plants designated by the Korean Ministry of Environment were two taxa, including Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. and Epilobium hirsutum L. The rare plants numbered 5 taxa designated by National Institute of Biological Resources were also found. 20 taxa of Korean endemic plants including Corydalis alata B.U.Oh & W.R.Lee, Iris odaesanensis Y.N.Lee, and Scrophularia cephalantha Nakai were investigated in this region. 40 taxa of floristic regional indicator plants in the 3rd to the 5th degrees were identified. Naturalized plants of 55 taxa were recorded, and 3 taxa of ecosystem disturbance plants, which were Ambrosia artemisiifolia L., Aster pilosus Willd., and Lactuca scariola L., also found.

• The Korea Journal of Herbology
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• v.28 no.4
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• pp.71-76
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• 2013

Objectives : The biological activities and compound contents of herbal medicine vary depending on manufacturing processes. In this study, we compared anti-inflammatory effects and compound contents of three kinds of multi-herbal extract HT008 produced by different manufacturing processes in order to determine chemical and biological equivalence. Methods : HT008 was produced by three different manufacturing methods: 1. Freeze dried extract of Eleutherococcus senticosus, Scutellaria baicalensis and Angelica sinensis (HT008 FD), 2. Spray dried extract of E. senticosus and S. baicalensis combined with reflux extract of A. sinensis (HT008 SD), 3. Spray dried extract of E. senticosus and S. baicalensis combined with supercritial fluid extract of A. sinensis (HT008 SF). Anti-inflammatory effects were evaluated using acetic acid induced pain model and ${\lambda}$-carageenan induced paw edema model. Compound contents were evaluated by HPLC quantitative analysis of standard compounds of HT008, eleutheroside E, baicalin, z-ligustilide. Results : HT008 FD, HT008 SD and HT008 SF significantly decreased acetic acid induced pain index and ${\lambda}$-carrageenan induced paw edema volume compared with that of control group. There was no significant difference in efficacy among the HT008 FD, HT008 SD and HT008 SF. Standard compound contents of HT008 FD, HT008 SD and HT008 SF were quantified within the range of Korean pharmacopoeia or other research. Conclusions : Three different manufacturing methods of multi-herbal extracts have been developed without noticeable difference in the efficacy or compound contents. The results might be used to establish manufacturing process and industrialization of herbal extracts.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1796-1800
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• 2014

Trienzyme digestion (AOAC Official Method 2004.05) procedure using protease, ${\alpha}$-amylase, and chicken pancreas conjugase was evaluated to determine its usefulness in the microbiological quantitation of total folate in foods. Folate values obtained by alkali hydrolysis (Korean Food Standards Codex) were compared to those obtained by the trienzyme method for four certified reference materials (CRM) representing diverse matrixes. Trienzyme treatment increased measurable folate from most CRM compared to levels found after alkali hydrolysis. The largest increases were observed with CRM 487 (pig liver, 5.8-fold) and CRM 121 (whole meal flour, 3.1-fold) after trienzyme digestion. Using trienzyme digestion method, total folate contents of raw and blanched edible plants were determined. Eleutherococcus senticosus ($146.9{\mu}g/100g$) showed the highest total folate content, followed by Aster glehni F. Schmidt ($142.8{\mu}g/100g$) and Ledebouriella seseloides H. Wolff ($140.4{\mu}g/100g$) on a wet weight basis. Blanching of samples resulted in an insignificant decrease in folate content for five samples and 11~63% reduction for nine samples. Our finding suggests that trienzyme digestion method is accurate for the determination of food folate in leafy vegetables.

• Korean Journal of Environment and Ecology
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• v.36 no.3
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• pp.220-256
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• 2022

The most important thing for conserving plant diversity in an area is to make an overall inventory of the plant species inhabiting the area. In particular, limestone areas are known for their high plant diversity and distribution of specific plants. Despite that, only a few have been designated as protected areas. This study investigated the vascular plants distributed in Goyangsan Mtn. and Munraesan Mtn., located in limestone areas of the central part of the Korean Peninsula. A field survey was conducted eight times from April to October 2021. As a result, we identified a total of 654 taxa comprising 113 families, 357 genera, 592 species, 15 subspecies, 44 varieties, and 3 formulas. They included four endangered wild plant species: Astilboides tabularis, Eleutherococcus senticosus, Cypripedium macranthos, and Epilobium hirsutum. Endemic plants in Korea were identified as 32 taxa. Floristic target plants were identified as 168 taxa, specifically 5 taxa of grade V, 41 taxa of grade IV, and 36 taxa of grade III. The red data plants included 2 taxa as "Endangered (EN)", 7 taxa as "Vulnerable (VU)", and 7 taxa as "Near threatened (NT)". A total of 41 taxa of naturalized plants were identified, and 4 of them were invasive alien plants. The surveyed vicinity of Goyangsan Mtn. and Munraesan Mtn. showed high plant diversity and contained core habitats for distribution of an endangered wild plant, Astilboides tabularis,in the limestone area. Moreover, both mountains contained a small population of Cotoneaster integerrimus. These findings confirm that the area has conservation values. Therefore, we propose to identify areas with high plant diversity and designate them as special protected areas.