• Title/Summary/Keyword: Electrospining

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Application of Biosystems Engineering to Tissue Engineering - Electrospining Technology to Fabricate Scaffolds for Bioartificial Organs - (생체조직공학에 바이오시스템공학의 응용 -인공장기용 지지체 제조를 위한 전기방사기술 -)

  • 정종훈
    • Journal of Biosystems Engineering
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    • v.29 no.4
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    • pp.367-374
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    • 2004
  • 2004년부터 본 학회지의 명칭이 바이오시스템 공학으로 바뀌었으나, 바이오시스템 공학의 분야가 명확하게 정의되지 않은 채 사용되고 있는 것 같다. 필자가 이를 정의하자면 바이오시스템 공학은 식물자원, 동물자원, 식품 등의 농업생명공학과 의료생명공학을 포함한 생물산업 분야에 필요한 생물, 기계, 전기 및 전자 등의 공학적 기술을 제공하고 다루는 응용공학이라 할 수 있다. 이에 바이오시스템 공학을 인공장기와 생체조직을 제조하는 생체조직공학의 한 분야에 응용하는 예로서, 인공생체조직용 나노단위 지지체(scaffold) 제조에 사용하는 전기방사기술과 그 시스템을 소개하고자 한다.(중략)

Micropatterning on Biodegradable Nanofiber Scaffolds by Femtosecond Laser Ablation Process (펨토초 레이저 절삭 공정을 이용한 생분해성 나노섬유 표면 미세 패터닝 공정)

  • Chung, Yongwoo;Jun, Indong;Kim, Yu-Chan;Seok, Hyun-Kwang;Chung, Seok;Jeon, Hojeong
    • Journal of the Korean institute of surface engineering
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    • v.49 no.6
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    • pp.555-559
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    • 2016
  • A biodegradable nanofiber scaffolds using electrospining provide fibrous guidance cues for controlling cell fate that mimic the native extracellular matrix (ECM). It can create a pattern using conventional electrospining method, but has a difficulty to generate one or more pattern structures. Femtosecond(fs) laser ablation has much interested in patterning on biomaterials in order to distinguish the fundamental or systemic interaction between cell and material surface. The ablated materials with a short pulse duration using femtosecond laser that allows for precise removal of materials without transition of the inherent material properties. In this study, linear grooves and circular craters were fabricated on electrospun nanofiber scaffolds (poly-L-lactide(PLLA)) by femtosecond laser patterning processes. As parametric studies, pulse energy and beam spot size were varied to determine the effects of the laser pulse on groove size. We confirmed controlling pulse energy to $5{\mu}J-20{\mu}J$ and variation of lens maginfication of 2X, 5X, 10X, 20X created grooves of width to approximately $5{\mu}m-50{\mu}m$. Our results demonstrate that femtosecond laser processing is an effective means for flexibly structuring the surface of electrospun PLLA nanofibers.

The hydrogen adsorption of electrospun carbon fibers web involving transition metal (전이금속 함유 전기방사 된 탄소섬유 웹의 수소 흡장)

  • Im, Ji-Sun;Kim, Ju-Wan;Park, Soo-Jin;Kim, Young-Ho;Lee, Young-Seak
    • 한국신재생에너지학회:학술대회논문집
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    • 2007.06a
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    • pp.77-80
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    • 2007
  • To increase the capacity of hydrogen adsorption, transition metals were adopted as catalyst. The PAN-based CNFs involving transition metal were obtained by electrospinning method and heat treatment. To study the surface of carbon fibers, SEM analysis was conducted. The mass of transition metals were spreaded or covered among CNFs. XRD and EDX analysis were used to confirm transition metals on the surface of carbon fibers. Volumetric method was used for studying the capacity of hydrogen adsorption on the carbon fibers involving transition metals. In this study. vanadium has the best characteristics among chromium, titanium, and copper for hydrogen adsorption.

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Fabrication of Electrospun Titania Nanofiber (전기방사법을 이용한 산화티탄 나노섬유의 제조)

  • Park, Sooil;Lee, Deuk-Yong;Lee, Myung-Hyun;Lee, Se-Jong;Kim, Bae-Yeon
    • Journal of the Korean Ceramic Society
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    • v.42 no.8 s.279
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    • pp.548-553
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    • 2005
  • $TiO_2$ nanofibers were fabricated by annealing electrospun $TiO_2$/PVP nanofibers for 3 h at $500^{\circ}C$ in air. Size and uniformity of electrospun $TiO_2$ nanofiber diameters were evaluated via XRD and SEM by varying electric field, PVP concentration, Ti tetraisopropoxide concentration and precursor flow rate. Experimental results revealed that the effect of PVP concentration on size and uniformity of electrospun $TiO_2$ nanofiber diameters was most profound, however, the other effects were relatively small. Uniform fibers with no beads were observed for the electrospun anatase titania nanofibers with a diameter of 170 nm.

Characterization of Nickel Oxide Nanofibers Obtained by Electrospinning

  • Park, Juyun;Kang, Yong-Cheol;Koh, Sung Wi
    • Journal of Integrative Natural Science
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    • v.11 no.1
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    • pp.14-18
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    • 2018
  • Nickel oxide nanofibers were synthesized by electrospinning with nickel(II) acetate tetrahydrate and polyvinylpyrrolidone and calcination process. The nanofiber shape was easily detected from the nanofibers with high Ni contents after calcined at $600^{\circ}C$ and the crystal structure of layer-by-layer growth was observed from SEM images at $900^{\circ}C$. XRD and TEM results showed metallic Ni and NiO structure were formed at nanofibers obtained at 600 and $900^{\circ}C$ and the crystallite size was calculated from 25 to 55 nm. The surface of nanofibers was fully oxidized from the deconvoluted Cu 2p and O 1s XPS spectra.

Adsorption Characteristic of L-tryptophan of Affinity Membrane (친화막의 L-tryptophan 흡착특성)

  • Byun, Hong-Sik;Hong, Byung-Pyo
    • Membrane Journal
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    • v.18 no.3
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    • pp.214-218
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    • 2008
  • Protain affinity membranes based on PES-BSA was preapared by the electrospinning method. The process problem caused by the electrospining was solved by using HFB having high solubility and boiling point. It was expecting that the mass production of protein affinity membrane would be possible with broad range of optimized temperature and humidity. BSA in the PES nanofiber was confirmed by the color change from colorless to violet during the biuret test. The buffer solution with DMSO showed that the amount of elution was 5 times higher than the one when the buffer solution without DMSO was used. This is due to the restriction effect of DMSO on the dissociation of L-tryptophan from BSA during the washing step.

A study on the differentiation of MC3T3-E1 incubated on the layer-built silica/polycaprolactone non-woven fabric produced by electrospinning (전기방사법으로 제조된 실리카/폴리카프로락톤 적층형 부직포에 배양한 골아 세포의 중식, 분화에 관한 연구)

  • AN, Min-Kuk;Kim, Kyoung-Hwa;Kim, Tae-II;Lee, Yong-Moo;Rhee, Sang-Hoon;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung;Han, Soo-Boo;Seol, Yang-Jo
    • Journal of Periodontal and Implant Science
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    • v.37 no.1
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    • pp.115-124
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    • 2007
  • Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well $1{\times}10^5$ concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air. 5% $CO_2$, $37^{\circ}C$). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of $1{\times}10^5$ concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p/0,05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.

Preparation and Characterization Study of PET Nanofiber-reinforced PEI Membrane, Investigation of the Application of Organic Solvent Nanofiltration Membrane (PET 나노섬유 강화 PEI 막의 제조 및 특성화 연구, 그에 따른 유기용매 나노여과막 가능성 검증)

  • Sung-Bae Hong;Kwangseop Im;Dong-Jun Kwon;Sang Yong Nam
    • Journal of Adhesion and Interface
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    • v.24 no.1
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    • pp.17-25
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    • 2023
  • In this study, waste polyethylene terephthalate (PET) was recycled to produce a support and then polyetherimide (PEI) was used for environmentally friendly organic solvent nanofiltration. The prepared composite membrane was first prepared by electrospinning a PET support, then casted on the support using PEI having excellent solvent resistance, and organic solvent nanoparticles using a Non-solvent Induced Phase Separation (NIPS) method. A filtration membrane was prepared. First, the fiber diameter and tensile strength of the PET scaffold prepared prior to membrane fabrication were identified through morphology analysis, and the optimal scaffold for the organic solvent nanofiltration membrane was identified. Afterward, the PET/PEI composite membrane prepared was checked for the DEA removal rate of Congo red having a molecular weight of 697 g/mol in ethanol to understand the performance as an organic solvent nanofiltration membrane according to the concentration of PEI. Finally, the removal rate of Congo red was 90% or more.