• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.05a
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• pp.57-57
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• 2004

• The Korean Journal of Zoology
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• v.17 no.4
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• pp.163-166
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• 1974

Malate dehydrogenases of ten bivalved mollusks consist of one or two isozyme, which are species-spcific. It is concluded that the electrophoretic character of the enzyme is available for identification of these ten bivalves.

• The Korean Journal of Zoology
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• v.15 no.3
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• pp.105-110
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• 1972

A cellulosse acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase (LDH and MDH) isozymes. The pattern of LDH and MDH isozymes in the tissues of the central nervous system of the six species of Anura examined are species specific and differ from those of mammals and birds. Both Rana nigromaculata nigromaculata and Rana nigromaculata coreana have two molecular forms of LDH and MDH, respectively, with almost the same pattern. Whole brain homogenate of Rana temporaria shows also a maximum of only two LDH isozymes. Both Bufo bufo asiaticus and Bombina orientalis have five molecular forms of LDH with an entirely different spacing on the zymograms, whereas Rana rugosa has three. Two molecular forms of MDH are present in all animals examined and one band is shown in olfactory lobe and mixture of cerebellum and medulla oblongata of Rana nigromaculata nigromaculata.

• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.371-379
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• 1988

To investigate on the biochemical characteristics of myofibrillar proteins between cold(pollack, salmon) and warm current fish (yellow corbina, shark), myofibrils and actomyosin were prepared, and their biological activities, effect of temperature on the myofibrillar ATPase activities and SDS-polyacrylamide gel electrophoretic patterns of myofibrils were compared. SDS-polyacrylamide gel electrophoretic analysis showed that electrophoretic patterns of myofibril vary from fish to fish. Difference in KCl concentration dependency of myofibrillar ATPase activities and ATPase activity- pH curve were found among fish species. Myofibrillar proteins from cold current fish showed higher specific activity at low temperature $(5^{\circ}C-10^{\circ}C)$ than those from warm current fish.

• Korean Journal of Veterinary Research
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• v.26 no.2
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• pp.265-276
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• 1986

The bovine serum factor influencing the growth of swine testicle (ST) cell and the END effect of hog cholera SN test was studied. Throughout the experimental studies. following results were obtained and summarized. 1. Bovine whole serum of 16(76.2%) and 4(19.0%) samples out of 21 have shown a positive ST cell growth and the END effect, respectively. However, all of 21(100%) and 8(38.1%) samples out of 21 serum supernatant fractions, prepared from the bovine whole serum, have shown positive ST cell growth and END effect, respectively. 2. In the SDS-polyacrylamide gel electrophoretic analysis of the bovine whole serum and the supernatant fractions, ST cell growth inhibiting factor was proved present in globulin fraction and in whole gel plate as a diffusible component. 3. The END ineffective component present in the whole serum and its supernatant fraction was proved to be BVDV neutralizing antibody. 4. The difference of osmolarity, optical density, pH, degree of precipitant formation following heat cold treatment, A/G ratio as we11 as electrophoretic pattern and NDV SN index of the samples were not correlated to the degree of 57 cell growth and to the END effectiveness.

• The Korean Journal of Mycology
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• v.14 no.2
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• pp.93-99
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• 1986

Isozyme patterns of leucine aminopeptidase, esterase and protein from 16 isolates of Ganoderma lucidum were observed by electrophoresis for characterization of the isolates. Even in the same isolate, the patterns of the isozymes from mycelium and cap were different. The esterase patterns from the mycelium could differentiate the isolates. Of 16 isolates, four isolates showed identical patterns. It was assumed that these had the same genetic background.

• The Korean Journal of Zoology
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• v.15 no.4
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• pp.193-205
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• 1972

Lactate and malate dehydrogenase isozymes in crude extracts of various tissues from 28 avian species were investigated by means of celluose acetate electrophoresis. In a given species the presence of two types of the malate dehydrogenase isozymes could be revealed in somatic tissues; one was characterized by fast electrophoretic mobility, and the other by slow mobility. Although the bird lactate dehydrogenase isozymes existed in a variety of species-specific molecular forms, they seemed to show the same pattern within the same genus and family.

• The Korean Journal of Zoology
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• v.17 no.3
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• pp.127-130
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• 1974

The lactate dehydrogenase isozymes in tissues of eight fresh water fishes were examined by cellulose acetate electrophoresis. The electrophoretic distribution of the isozymes showed clearly species-specific pattern. Various subbands were found more frequently in these fishes than in mammals. The isozyme pattern of muscle seems to tend to be the same with that of brain in these fishes. The fish lactic dehydrogenase suggested in many cases to be consisted of nonrandomly selected hybrids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.4
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• pp.334-341
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• 1990

The electrophoretic variations in protein and some enzymes during stratification of Panax ginsseng seeds were checked in relation to the differentiation and development of immature embryo, Seed-protein on PAGE showed eight major bands during the period of investigation, Four bands of Rf : 0.12, 0.24, 0.66 and 0,72 remained in dark stained condition, whereas the remainders (Rf : 0.40, 0.48, 0.56 and 0,76) became lighter in the staining condition as the treatment period was prolonged, In embryo, which was separately sampled from 75 days after the treatement, the only band of Rf 0.76 was not observed, In peroxidase, the number of bands was gradually increased and came to four bands at 45 days after treatment, and their staining color became gradually darker until the time of final samling. Also, the embryo had the same patterns as those in the intact seed. Esterase showed four bands during the period of investigation. As the treatment period was prolonged, the degree of staining condition came to darker in the band of Rf 0.36, but lighter in the band of Rf 0.56. The band of Rf 0.20 was not detected in the intact seed at 90 days after the treatment, but clearly observed in embryo. Glutamate dehydrogenase had only two bands: one (Rf : 0.16) was detected only at 60 days after the treatment and the other (Rf: 0.14) was always observed throughout the period, and showing simple pattern in the electrophoretic variation. Glutamate-oxaloacetate transaminase showed the only band of Rf 0.36 during the period of investigation and even in embryo, and showing the simplest pattern, indeed.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.75-81
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• 2015

Purpose: The present study was conducted to investigate whether the changes in structure and activity of antioxidative enzymes, superoxide dismutase(SOD) and catalase(CAT) present in the eyes appeared when they were repeatedly exposed to UV-A, and reveal the correlation of these changes. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-A of 365 min under the condition of 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-A irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. Results: SOD exposed repeatedly to UV-A showed the polymerization pattern through the electrophoretic analysis when it was repeatedly exposed under the condition of at least 1 hour a day however, the change of its activity was found to be less than 12%. On the other hand, CAT repeatedly exposed to UV-A showed reduced size of the electrophoretic band which indicated a structure denaturation and its activity was significantly decreased. In the case of that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band was shown in the electrphoretic analysis. Conclusions: From these results, it was revealed that the degree and pattern in structural denaturation of antioxidative enzymes differently appeared according to the type of enzyme, and the degree of structural denaturation was not always consistent with the reduction in enzyme activity.