• Title/Summary/Keyword: Electron micrograph

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DigitalMicrograph Script Source Listing for a Geometric Phase Analysis

  • Kim, Kyou-Hyun
    • Applied Microscopy
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    • v.45 no.2
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    • pp.101-105
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    • 2015
  • Numerous digital image analysis techniques have been developed with regard to transmission electron microscopy (TEM) with the help of programming. DigitalMicrograph (DM, Gatan Inc., USA), which is installed on most TEMs as operational software, includes a script language to develop customized software for image analysis. Based on the DM script language, this work provides a script source listing for quantitative strain measurements based on a geometric phase analysis.

Development of Dark Field image Processing Technique for the Investigation of Nanostructures

  • Jeon, Jongchul;Kim, Kyou-Hyun
    • Journal of Powder Materials
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    • v.24 no.4
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    • pp.285-291
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    • 2017
  • We propose a custom analysis technique for the dark field (DF) image based on transmission electron microscopy (TEM). The custom analysis technique is developed based on the $DigitalMicrograph^{(R)}$ (DM) script language embedded in the Gatan digital microscopy software, which is used as the operational software for most TEM instruments. The developed software automatically scans an electron beam across a TEM sample and records a series of electron diffraction patterns. The recorded electron diffraction patterns provide DF and ADF images based on digital image processing. An experimental electron diffraction pattern is recorded from a IrMn polycrystal consisting of fine nanograins in order to test the proposed software. We demonstrate that the developed image processing technique well resolves nanograins of ~ 5 nm in diameter.

Microscopic Observation of Kenaf by Optical and Scanning Electron Micrograph (Kenaf 구성 세포의 현미경적 관찰)

  • Yoon, Seung-Lak
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.2
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    • pp.47-54
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    • 2009
  • Anatomical characteristics of kenaf were investigated in transverse, radial and tangential direction by optical and scanning electron micrograph. Kenaf was made up of bast fibers, wood fibers, vessels and parenchyma cells. Bast fibers were long slender cells with different types of pits. The shape of wood fibers were in various ways and pointed at the ends. The pits were observed on the surface of bast fibers. Kenafs were diffuse and radial porous. and composed of solitary pores and two or three radial pore multiples. Various types of vessels were observed. The pits showed alternate pitting and larger diameter than other cells. Parenchyma cells were rectangular or square with different shapes of pith parenchyma cells compared to conventional types of parenchyma cells in wood. The number of pith on the surfaces were small.

Preparation of Ultra Fine Ammonium Perchlorate Crystals (초미세 크기의 과염소산암모늄 결정체 제조)

  • 김준형;임유진
    • Journal of the Korean Society of Propulsion Engineers
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    • v.8 no.1
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    • pp.1-7
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    • 2004
  • The precipitation of ultra fine ammonium perchlorate(UFAP) crystals from the N-methyl pyrrolidone(NMP) solutions of ammonium perchlorate(AP) was studied. The characteristics of the precipitated crystals were investigated by means of scanning electron micrograph(SEM), X-ray diffraction(XRD), and thermogravimetric analysis(TGA). When chloroform, methylene chloride and toluene were the precipitants, the product crystals had a mean particle size less than 2$\mu\textrm{m}$. The crystallographical property and thermal decomposition behavior of the prepared UFAP were almost the same as those of the commercial AP.

Development and Application of Natural Antimicrobial Agent Isolated from Grapefruit Seed Extract (Grapefruit 종자추출물로부터 광범위 항균제 개발 및 응용에 관한 연구)

  • 조성환;이상열;김재원;고경혁;서일원
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.33-39
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    • 1995
  • The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed coparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

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EFFECTS OF VARIOUS NSAIDS ON PROSTAGLANDIN SYNTHESIS AND CELLULAR CONFIGURATION OF HUMAN PERIODONTAL LIGAMENT CELLS (다양한 NSAID가 인간 치주인대세포의 prostaglandin 합성 및 세포 형태에 미치는 효과에 대한 연구)

  • Kim, Hyeok-Soo;Shim, Hae-Young;Chae, Chang-Hoon;Chang, Young-Il;Park, Jun-Woo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.5
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    • pp.455-463
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    • 2007
  • The present study was designed to evaluate effects of the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) on human periodontal ligament cells. Human periodontal ligament cells were grown from a cell line provided by Kyungpook National University. Effects of NSAIDs on the proliferation of human periodontal ligament cells were assessed using MTT assays. And then $PGE_2$ concentrations were determined by ELISA and the changes of cellular configuration were found by electron micrograph. The results were as follows; 1. The MTT assay demonstrated that the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) had not significant cytotoxic effect on human periodontal ligament cells. 2. NSAIDs inhibited the $PGE_2$ synthesis of human periodontal ligament cells compared with the control group. These inhibitory effects had no significant differences with NSAID type and concentration. 3. Electron micrographs of human periodontal ligament cells treated with NSAIDs showed more narrow and irregular shape.

The Phase Separation of Low Alkali Borosilicate Glass by Substituting $Li_2O$ for $Na_2O$ (산화리튬의 치환에 따른 붕규산 유리의 분상에 관한 연구)

  • 양중식
    • Journal of the Korean Ceramic Society
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    • v.18 no.1
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    • pp.27-34
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    • 1981
  • The phase separation of low-alkali borosilicate glass with the composition of $6.25Na_2O$.$18.75B_2O_3$.$75.00SiO_2$(mole%) substituting $Li_2O$ for $Na_2O$ was studied. The phase separation in the temperature range of transformation was examined with various heating temperatures and soaking times. Durability to water, thermal expansion and specific density of the specimen were investigated and the microstructure of the separated phase was also observed by transmission electron micrograph techniques. The maximum alkali extraction result with the best phase separation effect was obtained when $Na_2O$ of the base glass was replaced with $1.88Li_2O$ (mole %) and electron micrograph of carbon film replica of $1.88Li_2O$$4.37Na_2O$.$18.75B_2O_3$.$75.00SiO_2$ (mole %) glass showed that the glass consisted of homogeneous two phases. The minimum specific density was shown with the specimen treated at 57$0^{\circ}C$ and it was also shown that the longer the treating time the lower the specific density. The apparent activation energies of approximately 45 kcal/mole by the alkali extraction and 43kcal/mole by the thermal expansion method were derived from the Arrhenius plots, respectively.

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Artifacts Frequently Encountered in Electron Micrographs (생물시료의 전자현미경 시료 제작 및 관찰 과정에서 발생되는 인공물)

  • Park, Chang-Hyun;Cho, Kang-Yong;Uhm, Chang-Sub
    • Applied Microscopy
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    • v.35 no.1
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    • pp.1-13
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    • 2005
  • Fine photographs are essential in the electron microscopy. Artifacts can be introduced during all steps of electron microscopy; specimen processing, observation and printing. Every caution is necessary to avoid the artifact formation. In this review, the authors discussed the causes of various artifacts and suggested the solution to help the correct tissue handling and electron microscopic observations.

Physical Properties of Elastic Epoxies for High Voltage

  • Lee, Kwan-Woo;Park, Yong-Sung;Park, Dae-Hee
    • KIEE International Transactions on Electrophysics and Applications
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    • v.4C no.2
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    • pp.51-54
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    • 2004
  • In this paper, the thermal and mechanical properties of elastic epoxy for the application of high voltage products were investigated. Glass transition temperature (Tg) of elastic epoxies cannot be found from room temperature to 20$0^{\circ}C$ by DSC (Differential Scanning Calorimetry). Weight reduction occurred at 285$^{\circ}C$ and 451$^{\circ}C$ according to a thermogravimeter. The first temperature was affected by addictives and the second by epoxies characteristic. Maximum tensile strain showed 28.3kgf/$\textrm{cm}^2$/$\textrm{cm}^2$ at 20% of mechanical stress in addictives 35 (phr). The SEM (Scanning electron microscope) micrograph of the fracture surface observed void and tearing of elastic epoxy at addictives 35 (phr). On the other side, the SEM micrograph of the rigid epoxy showed a broken trace.

Use of Antibody Displayed Phage for the Detection of Dextran Using a Dipstick Assay and Transmission Electron Micrograph

  • Kim Du-Woon;Day Donal F.
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1316-1319
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    • 2006
  • An antibody displayed phage collection (SBAE-2R), screened from a human synthetic phage antibody library (Fab 21ox), was used for the determination of dextran. The dextran-binding affinity was determined by serologically specific transmission electron microscopy (TEM) and a paper dipstick assay. The phage collection was distributed over the dextrancoated grids with 39$\pm$25 phages/$\mu$m$^2$ on the grids. Phages were not seen on dextran-coated grids exposed to the Fab 2lox phage library. The phage collection (SBAE-2R) produced 54$\pm$3 color normalized intensity (N.I.) from 125 ppm to 1,000 ppm of dextran and 5$\pm$1 (N.I.) for 63 ppm of dextran in a paper dipstick assay. This research extends the analytical options for dextran analysis by antibody displayed phage with a minimum of equipment usage.