• Analytical Science and Technology
• /
• v.30 no.1
• /
• pp.10-19
• /
• 2017

Anabolic steroids have similar structures to testosterone, both of which promote the growth of muscle mass and increase strength. However, the side effects of anabolic steroid use may lead to heart attacks or strokes. Additionally, the excessive use of steroids inhibits the production of the sex hormones in the body via a negative feedback loop, which results in testicular atrophy in males and amenorrhea in females. Currently, the method of choice used to test for the presence of anabolic steroids is GC-MS. However, GC-MS methods require chemical derivatization of the steroid sample to ensure compatibility with the analytical method; therefore, analysis of many different samples is difficult and time consuming. Unlike GC-MS, the liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) method is suitable for many samples. Twenty-two different anabolic steroids were analyzed by LC-Q-TOF-MS with various collision energies (CE). Accurate mass spectral data were obtained using a Q-TOF-MS equipped with an electro-spray ionization source and operated in the positive MS/MS mode for several classes of steroids that are often the targets of testing. Based on the collected data, fragmentation pathways were carefully elucidated. The high selectivity and sensitivity of the LC-Q-TOF-MS instrument combined with these fragmentation pathways offers a new approach for the rapid and accurate screening of anabolic steroids. The obtained data from the 22 different anabolic steroids will be shared with the scientific community in order to establish a library to aid in the screening of illegal anabolic steroids.

• Analytical Science and Technology
• /
• v.29 no.1
• /
• pp.35-42
• /
• 2016

A new nano-composite carbon ink for the development of disposable dopamine (DA) biosensors based on screen-printed carbon electrodes (SPCEs) is introduced. The method developed uses SPCEs coupled with a tyrosinase modified nano-composite carbon ink. The ink was prepared by an “in-house” procedure with reduced graphene oxide (rGO), Pt nanoparticles (PtNP), and carbon materials such as carbon black and graphite. The rGO-PtNP carbon composite ink was used to print the working electrodes of the SPCEs and the reference counter electrodes were printed by using a commercial Ag/AgCl ink. After the construction of nano-composite SPCEs, tyrosinase was immobilized onto the working electrodes by using a biocompatible matrix, chitosan. The composite of nano-materials was characterized by X-ray photoelectron spectroscopy (XPS) and the performance characteristics of the sensors were evaluated by using voltammetric and amperometric techniques. The cyclic voltammetry results indicated that the sensors prepared with the rGO-PtNP-carbon composite ink revealed a significant improvement in electro-catalytic activity to DA compared with the results obtained from bare or only PtNP embedded carbon inks. Optimum experimental parameters such as pH and operating potential were evaluated and calibration curves for dopamine were constructed with the results obtained from a series of amperometric detections at −0.1 V vs. Ag/AgCl. The limit of detection was found to be 14 nM in a linear range of 10 nM to 100 µM of DA, and the sensor’s sensitivity was calculated to be 0.4 µAµM⁻¹cm⁻².

• Journal of the Korean Society for Precision Engineering
• /
• v.16 no.7
• /
• pp.15-23
• /
• 1999

With a remarkable expansion of communication industry, a pager or a cellular phone becomes a necessary communication device in modern society. However, a paging signal by a buzzer is often acted as an unpleasant noise in some places, thus necessitating a paging signal by a vibration motor. In this paper, a simpler type of a vibration motor, a coreless permanent magnet(PM) DC motor, is considered to substitute for the conventional vibration motors. Using an analytical method, electromagnetic field and operating torque were calculated for the given inner and outer PM type motors, and the results were confirmed by FEM analysis. As design parameters, number of PM poles, PM radial thickness, coil arc angle and number of winding stacks were chosen for sensitivity analysis. It shows that coil arc angle is the most important design parameter to increase the motor performance, without giving an adverse effect on motor weight, size and manufacturing cost. Based on the analysis of the outer PM type motor, an outer square PM type motor is proposed as the final design. Compared to the outer PM type, outer square type provides more flexibility to attach to the small size cellular phones. With the optimum design of square outer PM DC motor, it can successfully substitute the conventional types with less expensive manufacturing cost. better performance and smaller necessary space.

• Journal of the Microelectronics and Packaging Society
• /
• v.20 no.1
• /
• pp.21-26
• /
• 2013

This study presents new analysis method to predict bending behavior of packaging substrate structure by comparing finite element method simulation and measured curvature using 3D scanner. Packaging substrate is easily bent and deflected while undergoing various processes such as curing of prepreg and copper pattern plating. We prepare specimens with various conditions and measure contours of each specimen and compute the residual stresses on deposited films using analytical solution to find the principle of bending. Core and prepreg in packaging substrate are made up of resin and bundles of fiber which exist orthogonally each other. Anisotropic material properties cause peculiar bending behavior of packaging substrate. We simulate the bending deflection with finite element method and verify the simulated deflection with measured data. The plating stress of electrodeposited copper is about 58 MPa. The curing stresses of solder resist and prepreg are about 13 MPa and 6.4 MPa respectively in room temperature.

• Korean Journal of Optics and Photonics
• /
• v.16 no.5
• /
• pp.423-427
• /
• 2005

In this paper, we propose easily tooling method for Seidel third order aberration, which are not well utilized in actual design process due to the complication of mathematical operation and the difficulty of understanding Seidel third order aberration theory, even though most insightful and systematic means in pre-designing for the initial data of optimization. First, using paraxial ray tracing and Seidel third order aberration theory, spherical aberration coefficient is derived for a two-mirror system with a finite object distance. The coefficient, which is expressed as a higher-order nonlinear equation, consists of design parameters(object distance, two curvatures, and inter-mirror distance) and effective focal length(EFL). Then, the expressed analytical equation is solved by using a computer with numerical analysis method. From the obtained numerical solutions satisfying the nearly zero coefficient condition($<10^{-6}$), linear fitting process offers a linear relationship called the curvature linear equation between two mirrors. Consequently, this linear equation has two worthy meanings: the equation gives a possibility to obtain initial design data for optimization easily. And the equation shows linear relationship to a two-mirror system with a finite object distance under the condition of corrected third order spherical aberration.

• Food Science and Biotechnology
• /
• v.18 no.1
• /
• pp.113-117
• /
• 2009

A method was developed using enhanced liquid chromatography coupled with electrospray ionization mass spectrometry for the analysis and quantitation of 2 main potato glycoalkaloids, $\alpha$-chaconine, and $\alpha$-solanine, without any pre-concentration or derivatisation steps. Calibration curves generated by this technique exhibited a linear dynamic range from 0.025 to $50{\mu}g/mL$ and from 0.05 to $50{\mu}g/mL$ for $\alpha$-chaconine and $\alpha$-solanine, respectively. Matrix effects were evaluated by comparing calibration curves measured in matrix-matched and solvent-based systems. Ion suppression due to matrix effects was weak and extraction recoveries of 88 to 114% were obtained in different sample matrices spiked with analyte concentrations ranging from 15 to $35{\mu}g/mL$. Potatoes that had been genetically modified to tolerate glufosinate contained the same glycoalkaloid levels as their non-transgenic counterpart. We suggest complementing compositional comparison assessment strategy by validating quantitative analytical methods for the toxic glycoalkaloids in potato plants.

• Journal of Food Hygiene and Safety
• /
• v.31 no.3
• /
• pp.186-193
• /
• 2016

An analytical method was developed for the determination of oxathiapiprolin in agricultural commodities. Oxathiapiprolin is a new oomycide (fungicide of piperidinyl thiazole isoxazoline class) which controls downy mildew in cucurbits caused by Pseudoperonospora cubensis (oomycete plant pathogen). Agricultural commodities were extracted with acetonitrile and partitioned with dichloromethane to remove the interference, adjusting pH between 9 and 10 by 1 N sodium hydroxide. After purification by silica SPE cartridge to clean up the interference of organic compounds, they were finally quantified by HPLC-UVD (high performance liquid chromatograph ultraviolet detector) using a wavelength at 260 nm and confirmed by LC-MS (liquid chromatograph mass spectrometer) in electro-spray ionization positive ion mode. The standard calibration curve was linear with coefficients of determination ($r^2$) 1.00 over the calibration ranges (0.025-2.5 mg/L). Recoveries were ranged between 86.7 to 112.7%, with relative standard deviations less than 10% at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates. The overall results were determined and estimated according to the CODEX guidelines (CAC/GL40). The proposed method for determination of oxathiapiprolin residues in agricultural commodities can be used as an official method.

• Smart Structures and Systems
• /
• v.20 no.3
• /
• pp.329-342
• /
• 2017

In this paper, the dispersion characteristics of elastic waves propagation in sandwich nano-beams with functionally graded (FG) face-sheets reinforced with carbon nanotubes (CNTs) is investigated based on various high order shear deformation beam theories (HOSDBTs) as well as nonlocal strain gradient theory (NSGT). In order to align CNTs as symmetric and asymmetric in top and bottom face-sheets with respect to neutral geometric axis of the sandwich nano-beam, various patterns are employed in this analysis. The sandwich nano-beam resting on Pasternak foundation is subjected to thermal, magnetic and electrical fields. In order to involve small scale parameter in governing equations, the NSGT is employed for this analysis. The governing equations of motion are derived using Hamilton's principle based on various HSDBTs. Then the governing equations are solved using analytical method. A detailed parametric study is conducted to study the effects of length scale parameter, different HSDBTs, the nonlocal parameter, various aligning of CNTs in thickness direction of face-sheets, different volume fraction of CNTs, foundation stiffness, applied voltage, magnetic intensity field and temperature change on the wave propagation characteristics of sandwich nano-beam. Also cut-off frequency and phase velocity are investigated in detail. According to results obtained, UU and VA patterns have the same cut-off frequency value but AV pattern has the lower value with respect to them.

• Analytical Science and Technology
• /
• v.12 no.1
• /
• pp.68-74
• /
• 1999

The Concentrations of PCDDs/PCDFs and their precusors(chlorophenols, chlorobenzenes, PCB) were analyzed from the dioxin control device such as EP and SCR to know the emission patterns of these compounds and find the dioxin index compounds. The dioxin concentration increased 7 times in outlet part than inlet part of EP and the concentration of CBs, CPs and PCBs also were increased through this control device. These phenomia may be related to the operating temperature of Electroprecipitator(EP), which the operating temperature is near the $300^{\circ}C$, the method of the decreasing the operating temperature need to consider to prevent the formation of these compounds. In the selected catalytic reactor with wet scrubber(SCR+WS), these compounds were removed after passing the device over 90% for CPs, 30~40% for CBs and 60% for PCBs. But, the systematic study have to perform to reduce the formation of PCDDs/PCDFs and precusors.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.44 no.5
• /
• pp.45-54
• /
• 2007

Time/frequency analysis has been extensively used in biomedical signal processing. By extracting some essential features from the electro-physiological signals, these methods are able to determine the clinical pathology mechanisms of some diseases. However, this method assumes that the signal should be stationary, which limits its application in non-stationary system. In this paper, we develop a new signal processing method using Hilbert-Huang Transform to perform analysis of the nonlinear and non-stationary ventricular fibrillation(VF). Hilbert-Huang Transform combines two major analytical theories: Empirical Mode Decomposition(EMD) and the Hilbert Transform. Hilbert-Huang Transform can be used to decompose natural data into independent Intrinsic Mode Functions using the theories of EMD. Furthermore, Hilbert-Huang Transform employs Hilbert Transform to determine instantaneous frequency and amplitude, and therefore can be used to accurately describe the local behavior of signals. This paper studied for Return Of Spontaneous Circulation(ROSC) and non-ROSC prediction performance by Support Vector Machine and three parameters(EMD-IF, EMD-FFT) extracted from ventricular fibrillation ECG waveform using Hilbert-Huang transform. On the average results of sensitivity and specificity were 87.35% and 76.88% respectively. Hilbert-Huang Transform shows that it enables us to predict the ROSC of VF more precisely.