• BMB Reports
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• v.39 no.6
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• pp.649-655
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• 2006

The NSAID activated gene (NAG-1), a member of the TGF-$\beta$ superfamily, is involved in tumor progression and development. The over-expression of NAG-1 in cancer cells results in growth arrest and increase in apoptosis, suggesting that NAG-1 has anti-tumorigenic activity. This conclusion is further supported by results of experiments with transgenic mice that ubiquitously express human NAG-1. These transgenic mice are resistant to the development of intestinal tumors following treatment with azoxymethane or by introduction of a mutant APC gene. In contrast, other data suggest a pro-tumorigenic role for NAG-1, for example, high expression of NAG-1 is frequently observed in tumors. NAG-1 may be like other members of the TGF-$\beta$ superfamily, acting as a tumor suppressor in the early stages, but acting pro-tumorigenic at the later stages of tumor progression. The expression of NAG-1 can be increased by treatment with drugs and chemicals documented to prevent tumor formation and development. Most notable is the increase in NAG-1 expression by the inhibitors of cyclooxygenases that prevent human colorectal cancer development. The regulation of NAG-1 is complex, but these agents act through either p53 or EGR-1 related pathways. In addition, an increase in NAG-1 is observed in inhibition of the AKT/GSK-$3{\beta}$ pathway, suggesting NAG-1 alters cell survival. Thus, NAG-1 expression is regulated by tumor suppressor pathways and appears to modulate tumor progression.

• Journal of Life Science
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• v.14 no.1
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• pp.26-31
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• 2004

Redox factor-1 (Ref-1), known as a redox regulator, controls the DNA binding of AP-1 and is activated in HT29 colon cancer cells by hypoxia in vitro. REF-1 also increases tile DNA binding affinity of Hypoxia-inducible Factor-lalpha$ (HIF-lalpha$), HIF-like Factor (HLF) and early growth response-1 (Egr-1) which induce expression of the genes involved in angiogenesis, so that we speculate that REF-1 may play a role in hypoxia-induced angiogenesis. In this research we tried to detect novel proteins interacting with REF-1 using Yeast two-hybrid system using full-length REF-1 cDNA as bait. As result of such screening we detected 3 positive clones. DNA sequencing and GeneBank search revealed that one of the clones contained the same sequences as M.musculus cDNA for tioredoxin.

• Journal of Power System Engineering
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• v.15 no.5
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• pp.10-15
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• 2011

This paper describes the engine performance and combustion characteristics of a CRDI diesel engine, operated by electronically controlled diesel fuel injector with variable injection timing. This experiment focused on fuel injection timing and pressure about combustion characteristics of CRDI diesel engine. EGR was excepted because it would be furtherly analyzed with additional experiments. The experiment was conducted under the circumstance of engine torque for 4, 8, 12 and 16 kgf-m and fuel injection timing for $15^{\circ}$, $10^{\circ}$ and $5^{\circ}$ BTDC, at the engine speed of 1100, 1400, 1700 and 2000 rpm. Fuel injection was controlled to retard or advance initiation of the injection event by electronically controlled fuel injection unit injector on the personal computer. When fuel was injected into the cylinders of a CRDI diesel engine it would go through ignition delay before starting of combustion. Therefore, fuel injection timing of CRDI diesel engine had a significant effect upon performance and combustion characteristics. Depending on the injection timing the fuel consumption rate following the rotational speed and torque was 3~78 g/psh (1.7~30.6%). The range of fuel injection timing that resulted in low fuel consumption overall was BTDC 15-10 degrees.

• Biomolecules & Therapeutics
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• v.30 no.6
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• pp.593-602
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• 2022

The human papillomavirus (HPV)-18 E7 (E7) oncoprotein is a major transforming protein that is thought to be involved in the development of cervical cancer. It is well-known that E7 stimulates tumour development by inactivating pRb. However, this alone cannot explain the various characteristics acquired by HPV infection. Therefore, we examined other molecules that could help explain the acquired cancer properties during E7-induced cancer development. Using the yeast two-hybrid (Y2H) method, we found that the Elk-1 factor, which is crucial for cell proliferation, invasion, cell survival, anti-apoptotic activity, and cancer development, binds to the E7. By determining which part of E7 binds to which domain of Elk-1 using the Y2H method, it was found that CR2 and CR3 of the E7 and parts 1-206, including the ETS-DNA domain of Elk-1, interact with each other. As a result of their interaction, the transcriptional activity of Elk-1 was increased, thereby increasing the expression of target genes EGR-1, c-fos, and E2F. Additionally, the colony forming assay revealed that overexpression of Elk-1 and E7 promotes C33A cell proliferation. We expect that the discovery of a novel E7 function as an Elk-1 activator could help explain whether the E7 has novel oncogenic activities in addition to p53 inactivation. We also expect that it will offer new methods for developing improved strategies for cervical cancer treatment.

• Korean Journal of Applied Biomechanics
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• v.18 no.3
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• pp.61-69
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• 2008

The purpose of this study is to examine the success or failure on the balance beam in element group requirements posture which is bending salto side-ward tucked through kinetic analysis. The national team players were participated. The goal was to present training methods to coaches and athletes so as to provide scientifically useful information. The results from this study were summarized as below. When the performance was successful, the features of the body's center of gravity during the side somersault motion showed to spread from the center of the balance beam and the center of the gravity moved to the direction of the body's rotation. In the spring sections - event2 and 3, when the performance was successful, up/down fluctuation became more wider and increased air time. It supported the result that the projecting variable was higher than in failure trial. In addition, the right side hip joint angles and speed, and angular velocity as jumping up for a leap were larger than in failure trial. Those variables showed the optimal conditions for a leap. By increasing the speed of the upper limb from the shoulder and the speed of the shoulder joint angular velocity, the momentum was increased. Especially the right side shoulder joint angular velocity increased dramatically because the right leg was held. As to the side somersault motion, the angular momentum of successful trial with respect to x-axis was bigger than failed trial. It indicated that the increasing angular momentum with respect to x-axis was an important factor in flying motion. Besides, as to side somersault, the appropriate proportion of angular momentum with respect to y-axis and z-axis was a key to successful trails.

• Korean Journal of Applied Biomechanics
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• v.18 no.1
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• pp.137-146
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• 2008

The purpose of this study is to examine the causes of errors from EGR posture on the balance beam, which is bending flick-flack salto backward stretched national team players through kinematic analysis, and present training methods for them so as to provide scientifically useful information to coaches and athlete. Findings from this study are summarized below. The most important factors that affect the errors in boyd center position and speed change were the speed change of left and right body centers and the horizontal and vertical speed changes. The left and right acceleration changes were greater in failed posture than in successful posture. The horizontal and vertical accelerations in E3 and E5 were the key factors that affected the backward somersault and landing. The angular speed changes which varied between success and failure were notable in head and shoulder joints. In individual results. The section when the angular speeds of head and shoulder joint must be the greatest was E4. In this section, when the body is extending instantly in a bent posture, increasing the angular speeds of head, shoulder and hip joints can improve the duration of staying in the air and the rotation radius of a somersault.

• Journal of Plant Biology
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• v.36 no.4
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• pp.363-370
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• 1993

Experiments with non-nodulated alfalfa (Medicago sativa L. cv, Europe) plants grown in hydroponic cuiture, were carried out to estimate the remobiJization of nitrogen (N) reserves and to investigate the transported forms of reduced soluble-N in xylem during regrowth following shoot removal. Endogenous N remobilization were estimated by $^{15}N$ labelling and amino acids in xylem sap were analysed. The, $^{15}N$ contents of ~egr9wing leaves and stems increased as a result of remobilization of N reserves mainly from root ,system, Regrowing leaves were a stronger sink than regrowing stems, with about tWo-thirds of remobilized 15N being recoved in leaves. Endogenous N in lateral roots accounted for about 46% of the total N reserves used for regrowth, while tap roots accounted for 23%, About 72% of total endogenous N remobilized to regrowing shoot, occurred during the first 10 days of regrowth, The outflow of reduced soluble-N (mainly amino acids) was greater than that of protein-N, while the latter was the largest storage pool in tap root and lateral roots. It is suggested that amino acids-N was the most readily avaiable form of N reserves. Asparagine, which repre5ented about 75% of amino acids-N in xylem sap, was the main transported form of reduced N. Its relative contents, during the first 10 days of regrowth, decreased from 75% to 59%. This decline was accompanied by compensatory increase in the relative contents of asparatate and glutamine.