• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.229-237
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• 2018

This study was conducted to investigate the effect of natural mineral complex fed to laying hens during the summer in terms of the egg quality. A total of 480 laying hens (160 leghorns, 160 Korean native chickens and 160 silkies, all 62 weeks old), were used for 4 weeks. The four dietary treatments were as follows: CON) basal diet; MC1) CON + 0.1% natural mineral complex (NMC); NMC3) CON + 0.3% NMC, and NMC5) CON + 0.5% NMC. In terms of egg weight, the NMC3 and NMC5 treatments had significantly higher egg weights than that of the CON (p < 0.001) in all species. Eggshell strength in the NMC3 and NMC5 treatments was significantly higher than that of the CON in the leghorns on 2 week (p < 0.01). In the Korean native chickens, the eggshell strength for the NMC1, NMC3 and NMC5 treatments was significantly higher than that of the CON during all periods. In 4 week, the eggshell strength for the CON and NMC1 treatment was significantly higher in the leghorns (p < 0.001), but the MC treatments had a significantly higher eggshell strength than that of the CON in Korean native chickens. Egg production was significantly improved in all the treatments compared to the NMC3 treatment (p < 0.05), and hatchability was also improved compared to the CON (p < 0.05). In conclusion, supplementation of natural mineral complex in chicken diets influenced the egg quality during the summer season.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.839-844
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• 2012

This experiment was conducted to evaluate the effects of single or multi-particle size limestone on the egg shell quality, egg production, egg quality and feed intake in laying hens. A total of 280 laying hens (ISA brown) were used in this 10-wk trial. Laying hens were randomly assigned to 4 treatments with 14 replications per treatment and 5 adjacent cages as a replication (hens were caged individually). The experimental treatments were: i) L, basal diet+10% large particle limestone; ii) LS1, basal diet+8% large particle limestone+2% small particle limestone; iii) LS2, basal diet+6% large particle limestone+4% small particle limestone; iv) S, basal diet+10% small particle limestone. The egg production was unaffected by dietary treatments. The egg weight in S treatment was lighter than other treatments (p<0.05). The egg specific gravity in S treatment was lower than other treatments (p<0.05). The eggshell strength and eggshell thickness in S treatment were decreased when compared with other dietary treatments (p<0.05). The laying hens in LS1 and LS2 treatment had a higher average feed intake than the other two treatments (p<0.05). Collectively，the dietary multi-particle size limestone supplementation could be as efficient as large particle size limestone.

• Research in Plant Disease
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• v.15 no.2
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• pp.106-111
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• 2009

Before transplanting Chinese cabbage seedlings, two kinds of eggshell powder were blended into the soil of cabbage field where the club root pathogen, Plasmodiophora brassicae, was infested. The incidence of clubroot disease, the shoot and root growth of cabbages, and soil pH were examined four times at 10 to 13 days interval from transplanting Chinese cabbage. As results, the cabbages treated with eggshell powder without membrane showed the fastest growth in above ground part, and the lowest disease index for clubroot disease. The cabbages treated with eggshell powder with membrane showed better growth than the cabbages of non-treated check, but lower growth than those treated with eggshell powder without membrane. Soil pH started to increase from 3 weeks after soil blending of eggshell powder, and it reached to above 8.0. However, the soil pH of non-treated check stayed at around 6.8. In the experiment to compare the effect of eggshell powder with other calcium compounds, soil-blending of $CaCO_3$ resulted the lowest disease incidence of 1.7 and the registered fungicide, 'flusulfamide', and the resistant variety 'CR Green cabbage' followed with the incidence of 1.9. Cabbages of non-treated check scored the highest disease incidence, 3.4, and that of eggshell powder without membrane was as high as 2.7. However, the growth of Chinese cabbage showed the different pattern to the disease incidence. Chinese cabbages treated with eggshell without membrane recorded the highest average growth, around 2.1 kg. On the other hand, the average growth of CR Green Chinese cabbage was about 2.0 kg, that of flusulfamide-treatment plot was 1.7, and that of non-treated check was as low as 1.3 kg. Soil blending of eggshell powder without membrane did not inhibit the development of clubroot, but increased the growth of cabbage to a great extent. Therefore, it was confirmed that soil blending of eggshell powder before transplanting makes the Chinese cabbage culture possible even in the field infested with club root pathogen.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.12
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• pp.1095-1104
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• 2009

The objective of this research was to evaluate the feasibility of using oyster-shell and eggshell wastes for the stabilization of arsenic-contaminated soil. Artificial As(V) contaminated soil was mixed with 0~5% oyster-shell and eggshell wastes and each sample was incubated for 30 days in a controlled environment. The efficiency of each treatment was evaluated using various single extractants (1 N HCl, 0.1 N NaOH and 0.5 N $H_2SO_4$). The concentration of As(V) was reduced by 10% upon a 5% oyster-shell or eggshell waste treatments based on the Korea Standard Test method (1 N HCl extraction). Analogous trends were observed in the 0.1 N NaOH or 0.5 N $H_2SO_4$ extractions. In addition, the oyster-shell and eggshell waste treatments increased the pH of each soil from 6.54 (Control) to 7.62~7.94. The exchangeable Ca in each soil also sharply increased from 6.87 cmol(+)/kg (Control) to 12.77~20.18 cmol(+)/kg. Further research is needed to increase the effectiveness of the oyster-shell and eggshell waste for the stabilization of As(V) in the contaminated soil.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1300-1308
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• 2016

This study was conducted to evaluate the effects of phytogenic additive and antibiotic growth promoter in laying Japanese quails. One hundred and sixty five quails were divided into three groups of 5 replicates and 11 quails (8 females and 3 males) in each replicate. Treatment 1 was fed control diet, treatment 2 was fed control diet supplemented with 0.05% bacitracin methylene disalicylate as antibiotic growth promoter and treatment 3 was fed control diet supplemented with 0.1% phytogenic feed additive (PFA) for two periods of 3 weeks each from 37 to 42 weeks of age. Results showed that egg production, eggshell strength, eggshell weight, villus height and villus height to crypt depth ratio were significantly (p${\leq}$0.05) increased and feed consumption, feed conversion ratio, albumen, Haugh unit, cholesterol, low-density lipoprotein, alanine transaminase, gamma glutamyltransferase, alkaline phosphatase, high-density lipoprotein, triglyceride, number of goblet cell, crypt depth and intestinal bacterial population of Coliforms, Salmonella and E. coli were significantly (p${\leq}$0.05) decreased in PFA fed group. It is concluded that addition of PFA containing phytomolecules and organic acids as main ingredients could significantly improve the production parameters and the general health of laying quails as an alternative to antibiotic growth promoters.

• Korean Journal of Agricultural Science
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• v.45 no.3
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• pp.485-492
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• 2018

Two experiments were conducted to investigate the effect of feeding a diet supplemented with swine hair protein (SHP) on the nutrient digestibility of Korean native chickens and their egg production as well as on egg quality. In experiment 1, twenty roosters of the Hanhyup-3 strain were assigned to an individual cage to give 10 replicates per treatment (i.e., 0 and 10% of SHP), and the nutrient digestibility in response to the SHP supplementation was measured. In experiment 2, fifty-four layers of the Hy-Line strain were assigned to an individual cage to give 27 replicates per treatment (i.e., 0 and 10% of SHP), and egg production and egg quality were measured. In experiment 1, no significant difference (p > 0.05) was found in the digestibility of crude protein, crude fat, nitrogen-free extract (NFE), and crude fiber in response to the diet supplemented with SHP. In experiment 2, although no significant difference (p > 0.05) was found with the dietary treatments, the 10% SHP supplemented diet decreased (p < 0.05) the feed intake of laying hens. However, the SHP supplemented diet did not affect (p > 0.05) the laying rate, egg weight and feed conversion ratio of those fed the diet. Eggshell thickness and yolk color decreased (p < 0.05); however, eggshell strength, eggshell color, albumen height and Haugh units increased (p < 0.05) by feeding layers a diet supplemented with SHP. In conclusion, the results suggest that 10% SHP supplemented in a poultry diet could be a useful protein source.

• Korean Journal of Poultry Science
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• v.43 no.2
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• pp.97-103
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• 2016

This study aimed of effects of replacing maize with rice or brown rice on laying performance, egg quality, and apparent fecal digestibility of nutrient in Hy-Line Brown laying hens. A total of 200, 25-week old Hy-Line Brown laying hens were randomly assigned to one of 5 treatments, each of which was replicated 4 times. Four experimental diets that were formulated two different grains (brown rice or rice) with two inclusion levels of (15 and 30%) as a replacement of maize. The experimental period was 8 weeks. During the experiment, hens were provided with feed and water ad libitum and were exposed to a 16:8=L:D lighting schedule. There were no differences in feed intake, egg weight, and egg mass during the 8 weeks of the feeding trial among groups. However, hen-day egg production was greater (P<0.05) for Brown rice treatment groups than that for basal and rice treatment groups. In addition, the supplementation of brown rice or rice did not have an effect on eggshell strength, eggshell thickness, and HU during of the feeding trial (Table 4). However, egg yolk color was less (P<0.05) for basal treatment groups than that for among treatment group. ATTD of dry matter (DM) and crude fat were greater (P<0.05) for brown rice 15 treatment than for rice 30 treatment. Crude protein (CP) was greater (P<0.05) for brown rice 15 treatment than for basal treatment. In conclusion, brown rice or rice grains is a good alternative energy feedstuff and can be used in laying hens, totally replacing maize, without any negative effect on the laying performance, egg quality, and nutrient digestibility.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1028-1033
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• 2010

The effects of delta-aminolevulinic acid (DALA) or chito-oligosaccharide (COS) in layer diets on production performance, egg quality and hematological characteristics were investigated in this 8-week trial. Two hundred and seventy 27-week-old (Hy-Line brown) layers were randomly assigned to 5 treatments with 9 replications per treatment. Dietary treatments were i) Control (basal diet); ii) DALA1 (basal diet+DALA 2 mg/kg); iii) DALA2 (basal diet+DALA 4 mg/kg); iv) COS1 (basal diet+COS 100 mg/kg) and v) COS2 (basal diet+COS 200 mg/kg). In this study, no significant difference was observed in feed intake and egg shell quality (eggshell breaking strength and egg shell thickness) among treatments. The inclusion of DALA increased egg production during the first 4 weeks. Egg weight was increased in both DALA (quadratic, p<0.05) and COS (linear, p<0.05) treatments compared with the control treatment. Laying hens fed the DALA treatment had an increased yolk color unit (quadratic, p<0.05) and haugh unit (linear, p<0.05) compared with the control group. Similarly, these characteristics were also affected by COS treatments, with both values being linearly increased (p<0.05) in COS treatments compared with the control treatment. Additionally, birds fed DALA treatments significantly increased (quadratic, p<0.05) the number of RBC, WBC and lymphocytes compared with the control treatment. Dietary DALA supplementation linearly increased (p<0.05) the serum iron concentration at the end of the 8th week. The inclusion of COS increased (linear, p<0.05) the concentration of RBC, WBC and lymphocytes compared with the control treatment. In conclusion, dietary DALA at the lower dosage (2 mg/kg) could exert better effects in laying hens than higher dosage (4 mg/kg). Birds fed DALA supplemented diet had an increased iron availability, egg weight, eggshell quality and immunity. Moreover, the inclusion of COS (200 mg/kg) can increase egg weight, eggshell quality and immunity in laying hens. Therefore, both the utilization of COS and DALA could be considered as a new strategy for optimizing egg quality and health condition of laying hens.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.994-998
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• 2017

Objective: An experiment was conducted to determine the effect of superdosing phytase on productive performance and egg quality in laying hens. Methods: A total of 200 42-wk-old Hy-Line Brown laying hens were allotted into 1 of 5 dietary treatments with 5 replicates consisting of 8 hens per replicate. The positive control (PC) and negative control diets (NC) were prepared based on the recommended P levels in layer diets. Supplemental phytase was added to the negative control diet at 10,000 (SD10), 20,000 (SD20), or 30,000 (SD30) fytase units (FTU)/kg. Productive performance was summarized for 6 weeks from 42 weeks to 47 weeks of age. Egg quality was assessed from 4 eggs per replicate randomly collected at the conclusion of the experiment. Results: The SD20 treatment had greater (p<0.05) hen-day egg production than PC, NC, and SD10 treatment groups. There was no difference in hen-day egg production between SD20 and SD30 treatment groups. However, SD30 treatment had greater (p<0.05) hen-day egg production than PC treatment, but showed no difference in hen-day egg production as compared to NC and SD10 treatment groups. However, egg weight, egg mass, feed intake, and feed conversion ratio were not affected by dietary treatments. Egg quality including eggshell strength, eggshell color, egg yolk color, and haugh unit was not influenced by dietary treatments. Conclusion: Superdosing level of 20,000 FTU/kg phytase in diets has a positive effect on egg production rate, but no beneficial effect on egg quality in laying hens.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.135-140
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• 2002

An experiment was conducted to determine the effects of vitamin C supplementation on the performance and egg quality of layers. A total of 800 ISA Brown layers of 71 weeks old were assigned to one of the following diets containing 0, 3, 10, 50 or 100 ppm LG-vitamin C, 100ppm free ascorbic acid, 100 ppm Rovimix stay C-35 or 50 ppm LG-vitamin C supplemented with 100 ppm Zn methionine chelate. The feeding trial was conducted for flour weeks during the period of May to June when the average ambient temperature was $23.8^{\circ}C$ (5 :00 PM). Each treatment was replicated five times with 20 birds housed in 2 birds cage units. Vitamin C supplementation did not improve the overall performances (egg Production and egg Quality). Birds fed 100 ppm of LG vitamin C had the lowest egg Production while birds fed 100 ppm of Rovimix-35 had the lowest egg weight among birds fed different sources of vitamin C. Although supplementation of vitamin C over 50 ppm increased soft and broken egg Production, supplementation of 10 ppm LG vitamin C showed lowest soft and broken egg Production among all treatments. E99she11 thickness was highest in the treatment of 50 ppm LG vitamin C. In conclusion, supplementation of vitamin C at the level of 3 ~ 100 ppm did not significantly influence the laying performance and eggshell quality of laying hens under normal ambient(20 ~$26^{\circ}C$ ) condition.