• KSBB Journal
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• v.15 no.4
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• pp.342-345
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• 2000

A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.566-569
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• 2005

A novel purification method was developed for producing homoharringtonine from Cephalotaxus koreana, to guarantee high purity and yield. Our simple, efficient procedure for isolating and purifying homoharringtonine from C. koreanabiomass consisted of solvent extraction, synthetic adsorbent treatment, low-pressure chromatography, followed by high performance liquid chromatography (HPLC). The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and dramatically increased the yield and purity of crude homoharringtonine for high-performance liquid chromatography (HPLC) purification steps compared with alternative processes. Homoharringtonine could be obtained simply with high yield and purity from biomass using this purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification. Purified homoharringtonine was identified and characterized.

• The Transactions of the Korean Institute of Power Electronics
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• v.13 no.1
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• pp.70-77
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• 2008

Conventional PDP power system, which commonly uses two isolation transformer, consists of sustaining power ($V_S$), addressing power($V_A$), and Multi power($V_M$). Because each of these power conversion circuits use transformer and control IC, there are several defects: decrease of efficiency, rise of cost, and parts stress. This paper is proposed the method which operates PDP power system only with one transformer. The proposed method contributes not only to high-efficient performance of the DC/DC power stage and improvement of reliability but also to reduction of cost by reducing volume and size. Also, proposed method is proper to Address Display-period Separation(ADS) which is one of the driving methods of PDP. Superiority of the proposed method is proved by comparison with conventional method and theoretical, experimental analysis.

• Korean Journal of Geomatics
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• v.3 no.1
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• pp.67-72
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• 2003

We have seen rapid changes in road systems and networks in urban areas due to fast urbanization and increased traffic demands. As a result, many researchers have put greater importance on extraction, correction and updating of information about road systems. Also, by using the various data on road systems and its condition, we can manage our road more efficiently and economically. Furthermore, such information can be used as input for digital map and GIS analysis. In this research, we used a high resolution aerial photo of the roads in Seongnam area. First, we applied the top-hat filter to the area of interest so that the road markings could be extracted in an efficient manner. The lane separation lines were selected, considering the shape similarity between the selected lane separation line and reference data. Next, we extracted the roads in the urban area using the aforementioned road marking. Using this technique, we could easily extract roads in urban area in semi-automatic way.

• Resources Recycling
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• v.19 no.5
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• pp.3-12
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• 2010

Platinum group metals with high purity are important raw materials for high technology industry. Since there are no domestic ores containing PGMs, it is of importance to develop a separation process by which ultra pure PGMs can be recovered from diverse spent resources. Data on the chemical properties and dissolution behavior of PGMs were obtained from the literature. A method for the thermodynamic analysis of the HCl solution with chlorine gas was introduced. Utilizing the difference in the chemical properties and dissolution behavior of PGMs would lead to an efficient separation process to recover ultra pure PGMs.

• Proceedings of the IEEK Conference
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• 2001.10a
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• pp.730-733
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• 2001

Separation of PET and PVC is a key technology to achieve effective plastics recycling but no efficient and economically feasible method has been developed yet. The application of flotation was investigated by many researchers but the causes of the selectivity were not clarified. This paper described the adsorption mechanism of wetting agents onto plastics, using the agents which have various polarity and hydrocarbon chain length. It was found that (1) hydrophobic interaction played a predominant role for the adsorption, (2) anionic wetting agents could be adsorbed onto negatively charged plastics with the polar radicals oriented outer part of the plastics, then often depressed plastics more effectively than cationic agents, and (3) PET and PVC could be separated with dodecyamine hydrochloride and sodium dodecyl- sulfonate in the concentration ranges of 1.0$\times$10$^{[-10]}$ $^{6}$ -5.0$\times$10$^{[-10]}$ $^{5}$ and 2.0$\times$10$^{[-10]}$ $^{6}$ -1.0$\times$10$^{[-10]}$ $^{5}$ mo1/1, respectively.

• Journal of the Korean Chemical Society
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• v.39 no.8
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• pp.635-642
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• 1995

Improved sample introduction system has been investigated for the determination of volatile organic compounds in water using a purge & trap preconcentration apparatus and a capillary gas chromatography/mass spectrometry. The present limitations associated with the moisture control module and cryorefocusing system suggested by EPA were discussed. To solve the problems such as improper separation of peaks due to the adsorption of water and contamination of purge & trap system, a more efficient connection system between the purge & trap apparatus and the gas chromatograph was introduced and the optimum operational conditions were suggested. A carbopack B/carboxen 1000 and 1001 trap was used for the purge & trap procedure and a custom made crosslinked dimethyldiphenylpolysiloxane capillary column was used for the separation of compounds. Accuracy and precision of the method suggested in this report were examined and the method detection limit of each compound was proposed for the simultaneous determination of 54 volatile organic compounds in water.

• The KIPS Transactions:PartB
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• v.9B no.5
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• pp.643-652
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• 2002

This paper proposes an efficient hybrid fixed-point (FP) algorithm for improving performances of the independent component analysis (ICA) based on neural networks. The proposed algorithm is the FP algorithm based on secant method and momentum for ICA. Secant method is applied to improve the separation performance by simplifying the computation process for estimating the root of objective function, which is to minimize the mutual informations of the independent components. The momentum is applied for high-speed convergence by restraining the oscillation if the process of converging to the optimal solution. It can simultaneously achieve a superior properties of the secant method and the momentum. The proposed algorithm has been applied to the composite fingerprints and the images generated by random mixing matrix in the 8 fingerprints of $256\times{256}$-pixel and the 10 images of $512\times{512}$-pixel, respectively. The simulation results show that the proposed algorithm has better performances of the separation speed and rate than those using the FP algorithm based on Newton and secant method. Especially, the secant FP algorithm can be solved the separating performances depending on initial points settings and the nonrealistic learning time for separating the large size images by using the Newton FP algorithm.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.3
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• pp.155-162
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• 2014

In this paper, we propose an efficient shaking correction techniques for a moving vehicle image stabilization. The proposed shaking correction techniques was calculated cumulative histogram for the conversion and the separating information via color separation of video image frame of the input received. And it were to matching the histogram for match the color information as compensation result of the shaking vehicle video imaging. In this paper, the proposed the shaking correction techniques was obtained to the restoration result when compared to the existing shaking correction techniques that the smallest noise and better the naturalness of image through stabilization of luminance level and color level. Also, the imaging stabilization method was demonstrated the efficiency compared to other methods through to the real-time processing without the use of the memory.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.501-504
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• 2003

A novel prepurification method was developed aiming at increasing yield and purity, also reducing solvent usage for purification of paclitaxel. This method was a simple and efficient procedure, for the isolation and prepurification of paclitaxel from the biomass of Taxus chinensis, consisting of micelle formation, followed by two steps of precipitation. The use of a micelle and precipitation in the prepurification process allows for rapid separation of paclitaxel from interfering compounds and dramatically reduces solvent usage compared to alternative methodologies. This prepurification process serves to minimize the size and complexity of the HPLC operations for paclitaxel purification. This process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. As much as possible, the process has been optimized to minimize solvent usage, complexity, and operating costs.