• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 하계학술대회 논문집
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• pp.354-354
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• 2009

We have developed the prototypes of superconducting magnetic separation system with high temperature superconductor wire. This separation filter system consist of magnetized matrix SUS430 wire and acrylic frame. This study introduced rolled steel process coolant wastewater applied superconductor HGMS(High Gradient Magnetic Separation). HGMS treatment have acted high efficient method for various wastewater. We have surveyed superconducting magnetic separation technology and reviewed the status of related industries using applied superconductivity. In our basic preliminary experiment using HGMS, it was made clear that the fine para-magnetic particles in the wastewater obtained from rolling process of steel can be separated with high efficiency. We investigated the ability of magnetic flock formation, which used inorganic materials and polymer coagulants. We had a purpose to remove SS of coolant at steel factory. Maximum coagulation remove rate of SS 98%. Removing ratio of $Fe_3O_4$ fine particles in wastewater showed over than 99% in the wastewater containing magnetic fines after four times of repetition of separation.

• 약학회지
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• 제21권2호
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• pp.110-114
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• 1977

The purified .omicron.-iodobenzoic acid-$^{131}$ I, sodium iodide-$^{131}$I and mixtures of those compounds were applied to radio paper or radio thin layer chromatography to select the developing solvents with useful separation efficiency. The separation efficiencies were checked by radiochromatogram sacnnings. It has been found that the two dimensional radio paper chromatogrphy using the solvent systems of 8% phenol and butanol-glacial acid-water (4:1:1 v/v) and the radio thin layer chromatography using alumina gel plate and butanol- glacial acetic acid- water (4:1:1 v/v) are both efficient. In this method, the Rf value of .omicron.-iodobenzoic acid-$^{131}$ I and the unbound $^{131}$ I is 0.48, 0.85 and 0.15, respectively.

• 한국가금학회:학술대회논문집
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• 한국가금학회 1999년도 제16차 정기총회및학술발표회
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• pp.11-33
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• 1999

This study was conducted to determine the embryonic stages for the isolation of the highest number of PGCs and to improve PGCs enrichment method. The primordial germ cells(PGCs) from different sources of chick embryos were isolated. The embryonic stage having the highest number of PGCs from each sources was selected ; 1-day-old embryos for germinal crescent (stage 6-8), 2.5-day-old embryos for blood (stage 17-18) and 5.5-day-old embryos for gonad (stage 27-28). The number of PGCs from one embryonic germinal crescent, blood and gonad was about 87$\pm$1.8, 103$\pm$4.0, and 932$\pm$10.9, respectively. The viability of PGCs after Ficoll from each sources was similar, showing approximately 70%. the PGCs enrichment method was improved using Ficoll density gradient centrifugation. After this step the purity of PGCs from germinal crescent, blood, and gonad was 45$\pm$9.10%, 85$\pm$1.18%, and 86$\pm$0.19%, respectively. Also, PGCs were picked up by mouth pipette to improve the purity. This improved method for the separation of PGCs from different sources will serve as a useful too to preserve the foundation stocks of poultry and to produce germline chimeras.

• 한국축산식품학회지
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• 제36권6호
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• pp.769-778
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• 2016

In this study, we improved the eggshell-membrane separation process by separating the shell and membrane with EDTA solution, evaluating effects of three different extraction solutions (acetic acid, EDTA, and phosphate solution), and co-purifying multiple eggshell proteins with two successive ion-exchange chromatography procedures (CM Sepharose Fast Flow and DEAE Sepharose Fast Flow). The recovery and residual rates of eggshell and membrane separated by the modified method with added EDTA solution were 93.88%, 91.15% and 1.01%, 2.87%, respectively. Ovocleidin-116 (OC-116) and ovocalyxin-36 (OCX-36) were obtained by loading 50 mM Na-Hepes, pH 7.5, 2 mM DTT and 350 mM NaCl buffer onto the DEAE-FF column at a flow rate of 1 mL/min, ovocleidin-17 (OC-17) was obtained by loading 100 mM NaCl, 50 mM Tris, pH 8.0 on the CM-FF column at a flow rate of 0.5 mL/min. The purities of OCX-36, OC-17 and OC-116 were 96.82%, 80.15% and 73.22%, and the recovery rates were 55.27%, 53.38% and 36.34%, respectively. Antibacterial activity test suggested that phosphate solution extract exhibited significantly higher activity against the tested bacterial strains than the acetic acid or EDTA extract, probably due to more types of proteins in the extract. These results demonstrate that this separation method is feasible and efficient.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.522-525
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• 2002

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

• 한국항공우주학회지
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• 제34권5호
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• pp.19-29
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• 2006

본 연구에서는 비행중인 유도무기의 기체분리에 대한 수치적 해석을 수행하였다. 기체의 정확한 분리운동을 모사하기 위해 삼차원 공간에서 각각의 물체가 연결되어 움직이는 6자유도 운동방정식을 유도하였으며, 물체를 둘러싼 격자계의 독립적인 처리를 위해 개발된 비정렬 중첩격자기법과 연계하였다. 개발된 해석기법은 유도무기의 받음각이 0도와 5도인 경우의 기체분리운동 해석에 적용되었으며, 이를 통해 본 연구에서의 해석기법이 다중 물체간의 상대운동이 있는 비정상 유동장을 해석하는데 효과적임을 알 수 있었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.260-263
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• 2000

대량발효 후 미세여과막과 MWCO 100K, 50K의 한외여과막을 이용한 막분리시스템을 적용하여 MTGase를 분리한 결과 pore size 1.6, $0.7\;{\mu}m$의 cellulose fiber 재질의 예비여과막과 $0.45\;{\mu}m$ cellulose acetate재질의 미세여과막을 이용하여 얻어진 분리액을 MWCO 100K와 50K를이용하여 농축시킨 결과 enzyme의 농도가 1.29 units/ml, 효소 비활성도는 약 0.2 units/mg protein 으로 나타났으며 초기 배양액에 비해 3.7배의 농축효과를 보였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2002년도 추계학술대회 논문집(I)
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• pp.266-271
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• 2002

The purpose of this paper is to propose the efficient method of structuring the standardization strategies of the logistics in korea. It is necessary to plan the long-short terms policy which can be covered with the various fields in the logistics. Also, It must be composed of the separation of the role and cooperation among the each division. In addition, public relations and education is need to be the standardization of the logistics. Finally, we make effort to change the national standardization to the international standardization.

• 한국지능시스템학회논문지
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• 제13권2호
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• pp.200-208
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• 2003

본 연구에서는 효율적인 학습규칙의 신경망 기반 독립성분분석기법을 이용한 영상신호의 분리와 특징추출을 제안하였다. 제안된 학습규칙은 할선법과 모멘트를 이용한 조합형 고정점 학습알고리즘이다. 여기서 할선법은 독립성분 상호간의 정보를 최소화하기 위한 목적함수의 최적화 과정에서 요구되는 1차 미분에 따른 계산을 간략화하기 위함이고, 모멘트는 최적화 과정에서 발생하는 발진을 억제하여 보다 빠른 학습을 위함이다. 제안된 기법을 $512\times512$의 픽셀을 가지는 10개의 영상을 대상으로 임의의 혼합행렬에 따라 발생되는 혼합영상의 분리에 적용한 결과, 뉴우턴법에 기초한 기존의 알고리즘과 할선법만에 기초한 알고리즘보다 각각 우수한 분리률과 빠른 분리속도가 있음을 확인하였다. 또한 $256\times256$ 픽셀의 10개 지문상과 $480\times225$ 픽셀의 지폐영상에서 선택된 각각 10,000개의 3가지 영상패치들을 대상으로 적용한 결과, 제안된 기법은 뉴우턴법이나 할선법의 알고리즘보다도 빠른 특징추출 속도가 있음을 확인하였다. 한편 추출된 $16\times16$ 펙셀의 160개 독립성분 기저벡터 각각은 영상 각각에 포함된 공간적인 주파수 특성과 방향성을 가지는 경계 특성이 잘 드러나는 국부적인 특징들임을 확인하였다.

• 한국지능시스템학회논문지
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• 제12권3호
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• pp.210-218
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• 2002

본 연구에서는 근사화된 학습알고리즘의 신경망 기반 독립성분분석에 의한 효율적인 복합영상 분리기법을 제안하였다. 제안된 학습알고리즘은 엔트로피 최적화론 위한 목적함수의 판을 구하기 위해, 도함수 계산을 요구하는 뉴우턴법 대신 단순히 함수 값만을 이용하여 계산을 근사화한 할선법 기초한 고정점 알고리즘이다. 이렇게 하면 뉴우턴법에서 요구되는 복잡한 도함수의 계산과정을 간략화 할 수 있어 고정점 알고리즘의 독립성분분석이 가지는 학습성능을 더욱 더 개선시킬 수 있다. 제안된 학습알고리즘의 독립성분분석 기법을 500개의 샘플을 가지는 4개 신호와 $512{\times}512$의 픽셀을 가지는 10개의 영상을 대상으로 임의의 혼합행렬에 따라 발생되는 복합신호 및 복합영상들을 시뮬레이션하였다. 시뮬레이션 결과, 기존의 뉴우턴법에 기초한 고정점 알고리즘의 분석기법보다 빠른 학습속도와 개선된 분리성능이 있음을 확인하였다. 특히 기존의 알고리즘에서 임의로 선정되는 초기값에 의존하는 학습성능과 대규모의 영상분리에서 발생될 수 있는 비현실적인 학습시간도 함께 해결할 수 있음을 확인할 수 있었다.