• Korean Journal of Food Science and Technology
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• v.14 no.2
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• pp.174-178
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• 1982

Various separation methods such as solvent extraction, chemical treatment and molecular distillation were tested for the separation of tocopherols and sterols from soy oil scum. The end products of these methods were tocopherol concentrates and sterol crystals. In the solvent extraction, purity and yield of tocopherols were 21.2% and 28.3%, and those of sterols were 69.2% and 2.6%. In the chemical treatment, purity and yield of tocopherols were 11.8% and 76.4% and those of sterols were 85.1% and 34.3% respectively. In the molecular distillation, purity and yield of tocopherols were 45.0% and 68.0%, and those of sterols were 49.3% and 57.0% respectively. The end products from the methods were characterized by HPLC. Based on the results of this study, the molecular distillation method was found to be more efficient than any other method tested.

• Journal of Biomedical Engineering Research
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• v.28 no.5
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• pp.601-608
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• 2007

2 Dimensional Gel Electrophoresis(2DGE) is an essentialmethodology for analysis on the expression of various proteins. For example, information for the location, mass, expression, size and shape of the proteins obtained by 2DGE can be used for diagnosis, prognosis and biological progress by comparison of patients with the normal persons. Protein spot matching for this purpose is comparative analysis of protein expression pattern for the 2DGE images generated under different conditions. However, visual analysis of protein spots which are more than several hundreds included in a 2DGE image requires long time and heavy effort. Furthermore, geometrical distortion makes the spot matching for the same protein harder. In this paper, an iterative algorithm is introduced for more efficient spot matching. Proposed method is first performing global matching step, which reduces the geometrical difference between the landmarks and the spot to be matched. Thus, movement for a spot is defined by a weighted sum of the movement of the landmark spots. Weight for the summation is defined by the inverse of the distance from the spots to the landmarks. This movement is iteratively performed until the total sum of the difference between the corresponding landmarks is larger than a pre-selected value. Due to local distortion generally occurred in 2DGE images, there are many regions in whichmany spot pairs are miss-matched. In the second stage, the same spot matching algorithm is applied to such local regions with the additional landmarks for those regions. In other words, the same method is applied with the expanded landmark set to which additional landmarks are added. Our proposed algorithm for spot matching empirically proved reliable analysis of protein separation image by producing higher accuracy.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1751-1757
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• 2007

Elastin-like polypeptides (ELPs) undergo a reversible inverse phase transition upon a change in temperature. This thermally triggered phase transition allows for a simple and rapid means of purifying a fusion protein. Recovery of ELPs-tagged fusion protein was easily achieved by aggregation, triggered either by raising temperature or by adding salt. In this study, levansucrase has been used as a model enzyme in the development of a simple one-step purification method using ELPs. The levansucrase gene cloned from Pseudomonas aurantiaca S-4380 was tagged with various sizes of ELPs to functionally express and optimize the purification of levansucrase. One of two ELPs, ELP[V-20] or ELP[V-40], was fused at the C-terminus of the levansucrase gene. A levansucrase-ELP fusion protein was expressed in Escherichia coli $DH5{\alpha}$ at $37^{\circ}C$ for 18 h. The molecular masses of levansucrase-ELP[V-20] and levansucrase-ELP[V-40] were determined as 56 kDa and 65 kDa, respectively. The phase transition of levansucrase-ELP[V-20] occurred at $20^{\circ}C$ in 50 mM Tris-Cl (pH 8) buffer with 3 M NaCl added, whereas the phase transition temperature ($T_t$) of levansucrase-ELP[V-40] was $17^{\circ}C$ with 2 M NaCl. Levansucrase was successfully purified using the phase transition characteristics of ELPs, with a recovery yield of higher than 80%, as verified by SDS-PAGE. The specific activity was measured spectrophotometrically to be 173 U/mg and 171 U/mg for levansucrase-ELP[V-20] and levansucrase-ELP[V-40], respectively, implying that the ELP-tagging system provides an efficient one-step separation method for protein purification.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.3
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• pp.176-185
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• 1996

Transformin the huge size of drawings into a suitable format for CAD system and recognizng the contents of drawings are the major concerans in the automated analysis of engineering drawings. This paper proposes some methods for text/graphics separation, symbol extraction, vectorization and symbol recognition with the object of applying them to electronic cirucit drawings. We use MBR (Minimum bounding rectangle) and size of isolated region on the drawings for separating text and graphic regions. Characteristics parameters such as the number of pixels, the length of circular constant and the degree of round shape are used for extracting loop symbols and geometric structures for non-loop symbols. To recognize symbols, nearest netighbor between FD (foruier descriptor) of extractd symbols and these of classification reference symbols is used. Experimental results show that the proposed method can generate compact vector representation of extracted symbols and perform the scale change and rotation of extracted symbol using symbol vectorization. Also we achieve an efficient searching of circuit drawings.

• 한국전산유체공학회:학술대회논문집
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• 2001.10a
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• pp.132-137
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• 2001

We used In-line orifice mixer for efficient chemicals mixing in water treatment. The method of using In-line orifice mixer has been already proved the improvement of water treatment efficiency. Code of computational fluid dynamics for numerical analysis was performed using FLUENT, a commercial code. As variable for exactly standardizing, a proper ratio between an outer diameter of deflector and a diameter of pipe, the distance between deflector and orifice, a determination of orifice diameter fur an optimal mixing, a distance between injection nozzle's position and cone, Numerical study has been performed for optimal standard and analyzed flow field on a basis of turbulent intensity in an orifice downstream.

• Structural Engineering and Mechanics
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• v.61 no.2
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• pp.183-192
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• 2017

Seismic pounding between adjacent buildings with inadequate separation and different dynamic characteristics can cause severe damage to the colliding buildings. Efficient estimation of the maximum pounding force is required to control the extent of damage in adjacent structures or develop an appropriate mitigation method. In this paper, an analytical approach on the basis of statistical relations is presented for approximate computation of extreme value of pounding force between two adjacent structures with equal or unequal heights subjected to stationary and non-stationary excitations. The nonlinearity of adjacent structures is considered using Bouc-Wen model of hysteresis and the pounding effect is simulated by applying the nonlinear viscoelastic model. It is shown that the proposed approach can significantly save computational costs by obviating the need for performing dynamic analysis. To assess the reliability and accuracy of the proposed approach, the results are compared with those obtained from nonlinear dynamic analysis.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.2
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• pp.187-196
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• 1996

This paper introduces the concept of fixed weights and proposes an algorithm for classification by adding this concept to vector space separation method in LVQ. The proposed algorithm is based on competitive learning. It uses fixed weightsfor generality and fast adaptation efficient radius for new weight creation, and L1 distance for fast calcualtion. It can be applied to many fields requiring adaptive learning with the support of generality, real-tiem processing and sufficient training effect using smaller data set. Recognition rate of over 98% for the train set and 94% for the test set was obtained by applying the suggested algorithm to on-line handwritten recognition.

• Journal of the Korean Ceramic Society
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• v.52 no.6
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• pp.489-496
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• 2015

In this study, novel $Ag_2Se$ sensitized $TiO_2$ nanocomposites were prepared by facile sonochemical assisted synthesis method. The as-prepared products were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), and N2 adsorption BET analysis. The as-prepared $Ag_2Se/TiO_2$ nanocomposites simultaneously possessed great adsorptivity for organic dyes and efficient charge separation properties. In the decolorization of rhodamine B, a significant enhancement in the reaction rate was observed for the $Ag_2Se/TiO_2$ nanocomposite compared to the cases of using pure P25 or $TiO_2$. The sonocatalysis activity was higher due to the greater formation of reactive radicals, as well as to the increase of the active surface area of the $Ag_2Se/TiO_2$ nanocomposite.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.459.1-459.1
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• 2014

Because both $TiO_2$ and ZnO has superior characteristic optically and electrically, there are various of research for these materials. However, they have large band gap energy which correspond with not visible light, but UV light. To make up for this disadvantage, Quantum dots (CdS, CdSe) which can absorb the visible light could be deposited on $ZnO/TiO_2$ nanostructure so that the the photoelectrochecmical cell can absorb the light that has larger region of wavelength. Both $TiO_2$ and ZnO can be grown to one-dimensional nanowire structure at low temperature through solutional method. Three-dimensional hierarcical $ZnO/TiO_2$ nanostructure is fabricated by applying these process. Large surface area of this structure make the light absorbed more efficiently. Through type 2 like-cascade energy band structure of nanostructure, the efficient separation of electron-hole pairs is expected. Photoelectrochemical charateristics are found by using these nanostructure to photoelectrode.

• Proceedings of the Membrane Society of Korea Conference
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• 1995.10a
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• pp.58-73
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• 1995

Membrane filtration is a promising technology for efficient solid/liquid separation in water purification. In FY 1991, the Ministry of Health and Welfare, Japanese Government launched a comprehensive research project "MAC 21" for development of membrane technology and its application to public water supply. The project was conducted by the Water Purification Process Association (WPPA), under the supervision of the Institute of Public Health. By the research project from FY 1991 to FY 1993, we confirmed that microfiltration (MF)/ultrafiltration (UF) technology was applicable to water purification and MF/UF was a effective method for the removal of such contaminants as particulate matter and coliforms. The Guideline Committee organized under the Technical Committee prepared a the guidelines on application of membrane system to small-scale public water supplies, based on the results as written above. The guidelines has been published in Dec., 1994 by WPPA.4 by WPPA.