The purification and characteristics of the biosynthesis enzyme of vitamin C from microorganisms related with kimchi fermentation were investigated to define vitamin C biosynthetic pathways in yeast. A yeast strain (Pichia onychis 16-4) which synthesizes vitamin C with galacturonic acid as substrate at high rate was isolated from kimchi. The enzyme $L-galactono-{\gamma}-lactone$ oxidase isolated from the yeast was purified and characterized. The specific activity of the crude enzyme was 7.26 unit/mg protein, which increased to 4,698 unit/mg protein with a chromatography of Sephacryl S-200HR; indicating a 647.1-fold level of purification. The molecular weights of the dissociated enzymes were estimated to be 31,000, 39,000, and 50,000 KD. Among the substrates tested, $L-galactono-{\gamma}-lactone$ was the most effective. The enzyme showed optimum activity ah pH 7.8 and 35c. The purified enzyme uses $O_2$ as the electron acceptor for oxidation of $L-galactono-{\gamma}-lactone$.
Journal of the Korean Regional Science Association
/
v.36
no.3
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pp.53-65
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2020
This study attempted to seek policy directions for development of sustainable urban industrial space through reviewing recent policies and projects of US cities that experienced urban industrial land replacement and industrial suburbanization. The policies and regulations for the urban industrial space preservation include the New York City's Industrial Business Zone, Chicago's Planned Manufacturing District, the no-net-loss framework in San Jose, and the subdivided and the state of the art in zoning systems in Philadelphia and Seattle. These policies and zoning systems have been effective for preventing the conversion of urban industrial lands to other uses, promoting business activities, and creating jobs for urban residents. In addition, the updated and innovative zoning systems allow for a wide variety of urban industrial spaces to meet newly emerging industrial demands. On the other hand, we suggested several policy implications through reviewing the cases of urban regeneration projects in Portland's Willamette Industrial Urban Renewal Area, Atlanta's BeltLine, and Milwaukee's Menomone Valley as follows: 1) updating and reinforcing spatial planning and policies for preservation and revitalization of urban industrial land; 2) considering existing businesses and local workers first; 3) setting up a participatory planning for local workers and residents; 4) preparing policies for brownfield cleanups.
Lee Seung-Woo;Han Seung-Hwan;Ko Suck-Bum;Kim Ji-Won
International Journal of Highway Engineering
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v.8
no.3
s.29
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pp.105-114
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2006
A popular alternative to extend the life of aged pavement is asphalt overlay. However, it has a very serious and inherent shortcoming in deterring a reflection crack. Although joint-rehabilitation and stress-relief techniques have been applied to deter such reflection cracks in aged pavement, the techniques had a limited success only in slowing down the progress of a reflection crack. Rubblization technique rubblizes the concrete pavement slab in situ and uses the rubblized slab as the base material. Then, pavement overlay is applied to finish off the rehabilitation of aged pavement. This rubblization technique has the advantage of solving the problem of reflection cracking completely. When rubblization technique is applied, the upper layer of aged concrete pavement is rubblized between 40mm-70mm in depth. However, the lower layer is typically rubblized more than 100mm in depth. Nevertheless, it is difficult to turn the entire concrete pavement of more than 30cm in depth into rubblized aggregate of appropriate size. Thus, a simulation experiment was carried out to find the appropriate rubblized depth, which avoids the reflection cracking and still maintains the function of subbase, by varying the depth of rubblized depth in loom increments of 0cm, 10cm, and 20cm. The result indicated the optimum rubblized depth was 10cm (Lee, 2006). Additionally, a small rubblizinge equipment was developed in order to derive the rubblization technique appropriate for thick concrete pavement. This equipment was tested out on an experimental pavement, which was constructed with the same standard and specification for the road in actual use, by varying its rubblizing head shape and energy as well as the effective area of rubblization. This experiment led to a prototype equipment for rubblization of thick concrete pavement. The prototype was put into use on a highway, undergoing a test construction and monitoring afterwards. This entire process was necessary for the validation of the proposed rubblization technique.
Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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2006.05a
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pp.164-170
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2006
This paper proposes a RSM-based hybrid evolutionary algorithm (RHEA) which combines the merits of the popular programs such as genetic algorithm (GA), tabu search method, response surface methodology (RSM). This algorithm, for improving the convergent speed that is thought to be the demerit of genetic algorithm, uses response surface methodology and simplex method. The mutation of GA offers random variety to finding the optimum solution. In this study, however, systematic variety can be secured through the use of tabu list. Efficiency of this method has been proven by applying traditional test functions and comparing the results to GA. And it was also proved that the newly suggested algorithm is very effective to find the global optimum solution to minimize the weight for avoiding the resonance of fresh water tank that is placed in the rear of ship. According to the study, GA's convergent speed in initial stages is improved by using RSM method. An optimized solution is calculated without the evaluation of additional actual objective function. In a summary, it is concluded that RHEA is a very powerful global optimization algorithm from the view point of convergent speed and global search ability.
Coal ash is generated from coal-fired thermal power plants every year. The remaining quantity of coal ash ends up in the landfills except for the recycled portion, and the existing ash pond capacity is limited almost. Currently, the difficulties are faced in building a new ash treatment plant because of the concerns about the environmental impacts of landfills at individual plant facilities. In terms of minimizing the environmental impact, the recycling and effective uses of coal ash are recognized as urgent issues to be challenged. Accordingly, this study examines the obstacles in expanding the recycling of the coal ash in South Korea and proposes solutions based on the case study analysis. The analysis results are as follows: 1) specific recycling guidelines and standards are required to be established in accordance with the contact medium (soil, ground water, surface water and sea water) and the chemical. 2) by providing the recognition environmentally safe in recycling the coal ash, transparency in establishing the planning stages and active communication with the community through promotion and research are essentially needed. 3) practical support system is required to encourage the power plant companies to use the coal ash as beneficial use.
In this paper, we develop a speech recognition system using a throat microphone. The use of this kind of microphone minimizes the impact of environmental noise. However, because of the absence of high frequencies and the partially loss of formant frequencies, previous systems developed with those devices have shown a lower recognition rate than systems which use standard microphone signals. This problem has led to researchers using throat microphone signals as supplementary data sources supporting standard microphone signals. In this paper, we present a high performance ASR system which we developed using only a throat microphone by taking advantage of Korean Phonological Feature Theory and a detailed throat signal analysis. Analyzing the spectrum and the result of FFT of the throat microphone signal, we find that the conventional MFCC feature vector that uses a critical pass filter does not characterize the throat microphone signals well. We also describe the conditions of the feature extraction algorithm which make it best suited for throat microphone signal analysis. The conditions involve (1) a sensitive band-pass filter and (2) use of feature vector which is suitable for voice/non-voice classification. We experimentally show that the ZCPA algorithm designed to meet these conditions improves the recognizer's performance by approximately 16%. And we find that an additional noise-canceling algorithm such as RAST A results in 2% more performance improvement.
Journal of the Korea institute for structural maintenance and inspection
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v.16
no.5
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pp.129-136
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2012
Temperature of fresh concrete can be effectively used to predict the strength of concrete being cured and make an informed decision for stripping the molds. A hygrothermograph and thermo-couple sensors that require an extensive wiring have been applied to measure a temperature of concrete at the early stage of the curing process on site. However, these methods have limits to provide the temperature data in real time due to harsh working environment including frequent cutting of wires. Therefore, this study is aiming at developing a device based on wireless sensor network to measure the temperature of concrete being cured in formwork. The result showed that the wireless sensor with probe type thermistor which is developed had the same temperature data compared to the existed wire type thermistor, and we confirmed the temperature history of concrete in real time for 28 days throughout the gateway by wireless network that collects the temperature data measured from specimens in laboratory. Also, the network device for transmission can be easily separated from the probe sensor part and reused consistently. If the wireless sensor network device developed uses in the field, the temperature management of concrete will be systematically conducted from at the early stage of the curing, and especially be effective for cold weather concrete construction. In addition, it will contribute to the establishment of advanced quality control system for concrete and productivity of supervisors on site will be increased in the future.
Broadcast encryption system is a cryptographic primitive that enables a sender to broadcast a message to a set of receivers in a secure channel. Out of previous proposed broadcast encryption systems, the most effective is the one that uses the Subset Difference(SD) method in a binary tree structure. The SD method has been realized by two underlying approaches: Pseudo-Random Generator(PRG) and Secret Sharing(SS). 2-SD method is the generalized version of the SD method by which two subsets of revoked receivers can be dealt with by one subset (in an SD-based broadcast encryption system). The primary advantage of the 2-SD method is to further reduce the size of transmission overhead, compared to the SD method. Until now, however, there is no known broadcast encryption system that is based on such a 2-SD technique using either PRG or SS basis. In this paper, we suggest a new 2-SD broadcast encryption system using the SS-based technique that was suggested by Jae Hwan Lee et al. in 2014[9]. The new system can reduce the size of ciphertext by 25% of the one in the previous SS-based broadcast encryption system. Also, on a theoretical note, ours is the first 2-SD broadcast encryption system that is provably secure.
In Korea, the Emergency Medical Service, EMS is provided by Fire Services with 119 EMS and all the public uses the service for free. Although it appears very successful and is respected nationally, structural problem EMS exist and are worsening. First of all, the "free riding effect" becomes increasingly problematic. Some argue that 30% of the demands is not urgent or emergent. The total number of demands is increasing even without the free riding effect. The Current EMS system itself cannot meet the increasing EMS demand. The medical aspect is so poor that the EMS cannot dispatch a medical specialist to the scene. The cardiac arrest resuscitation rate is only $1.24{\sim}9.9%$, compared to 40% in Boston, MA, USA. But due to the regulations and limitations of the Fire Service organization, it is difficult to secure enough EMS resources. To work out these problems, it needs a structural innovation. To secure enough resources and achieve higher medical performance we should invite the medical sector and the private sector into EMS arena by contracting partnerships with Fire Services and charging a reasonable EMS fee. We found through statistical test that any partnership system is more effective than fire-alone system and most countries around the world have partnership system rather than fire-alone system.
Journal of the Korean Society for Library and Information Science
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v.24
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pp.139-169
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1993
The major purpose of this study is to scrutinize and analyze various factors in numerous aspects of two different library systems for the visually handicapped, namely braille library and a special section for the visually handicapped in the public library system. The analyses may provide theoretical and conceptual framework on which strategies to enhance services for the visually handicapped is to be developed. More specifically, this study seeks (1) to examine a linkage between the degree of maintenance of staffs, collections, and equipment and the level of satisfactions of librarians and (2) to come up with more effective mechanisms of welfare services for the visually handicapped in each library system. This research is qualitative-descriptive and uses interview method. Fourteen libraries throughout the nation (4 public libraries with a reading room for the visually handicapped and 8 braille libraries) which had been in operation more than one year were selected as subjects for the research. Findings and results of the analyses are summarized as follows : 1. There seems to be general consensus that each of public and braille library has significant but, to a certain extent, different roles to play in order to enhance library services for the visually handicapped. Recruitment of qualified librarians, increase of the volume of collection, and enlargement of reading room space are noted as immediate concerns for the former. For the latter, it was suggested that increase of the number of staff for publishing, securing more equipments for pulication in braille, and wider scope as well as more variety of publications are the pressing problems at hand. 2. Both public and braille library employ one librarian for the visually handicapped who is required to have educational background in library science and special education. In addition, one must have competence in reading braille types. 3. Majority of reading rooms in those libraries is so small in physical aspect that size and number of seats are 66m2 and 15 seats respectively because circulation services are mainly in use due to the problem of inaccessibility which is commonly suffered by the visually handicapped. 4. Library services for the visually handicapped are heavily concentrated upon the large urban area. In fact, most of braille libraries are located in Seoul whereas a reading room for the visually handicapped in the public library system are exclusively placed in the large cities such as Inchon, Taejon, and Taegu. 5. All of the public library is neither equipped with necessary facilities to publish in braille nor secured supporting instruments which may facilitate library use for the visually handicapped. On the contrary, most of braille libraries are equipped with arrangements to publish in braille despite supporting devices are gererally lacking. 6. Consequently, provision of services In the public library is largely confined to reading and circulation of materials purchased from braille libraries while major task of braille libraries centers around publication and distributions of the reading materials in braille.
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