• 미생물학회지
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• 제20권3호
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• pp.119-124
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• 1982

As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4\;1.0g, \;sucrose\;30.0g, \;MgSo_4{\cdot}7H_2O\;0.5g, \;FeCl_3{\cdot}6H_2O\;1.0mg\;thiamine\;200{\gamma}g$, and distilled water 1000ml, pH6.5.

• 한국연초학회지
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• 제14권1호
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• pp.33-41
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• 1992

These studies were conducted to investigate effects of phytohormone and activated carbon on the growth and rooting of teratoma shoots induced from Nicotiana tabacum cv. NC2326 transformed by Aerobacterium tumefaciens C58. GA was effective for shoot elongation and reduction of multiple shoots from teratoma shoot, however, leaves of teratoma shoot cultured on the medium with GA were pointed. ABA was also effective in promoting shoot elongation, but was not for reduction of multiple shoots. Teratoma shoot cultured on the medium with 1 n activated carbon promoted shoot elongation and inhibited the number of shoots differentiated, but was grown as abnormal shoot. Addition of 1% activated carbon and 0.5mg/l BA to culture media was effective for shoot elongation and reduction of multiple shoot and for formation of round leaves as normal leaves. Though these shoots were inoculated on the rooting medium, they could not from roots but formed multiple shoots. Boric acid, myo-inositol and sucrose were also ineffective on the rooting of teratoma shoots.

• 한국수정란이식학회지
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• 제20권2호
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• pp.113-121
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• 2005

This study was conducted to investigate the effects of different hexoses (glucose, mannose, galactose and fructose) on in vitro development of parthenogenetic embryos in the pigs. When the parthnogenetic embryos were cultured in medium with concentrations of 5mM glucose or 1mM galactose, the rates of embyos developed to morula and blastocyst stages were significantly higher than those in another culture conditions (P<0.05). However, high concentration of galactose inhibited development to morula and blastocyst stages. Addition of hexoses at early stage of porcine parthenogenetic embryos were effective for in vitro development. Especially, the embryos cultured in medium with glucose at early stage were effective for development to 2-cell $(72\%)$ and blastocyst $(19\%)$ stages compared with embryo cultured without glucose. From the present results, it is suggested that development of porcine parthenogenetic embryos can improve in medium with 5mM glucose. The concentration of 1mM galactose was also effective for development of porcine parthenogenetic embryos. It also show that parthenogenetic embryos cultured with glucose at early stage can improve in vitro development.

• 미생물학회지
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• 제13권1호
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• pp.37-44
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• 1975

As a basic study to elucidate nutritional physiology and composition of synthetic medium of red rotting bacteria, Erwinia carotovora, of ginseng, the effects of hydrogen ion concentration, various kinds of carbon sources, nitrogen source, micrometallic salts and it's concentration on the gorwth of the bacteria were investigated and the results were as follows. Optimal pH in the basal medium for the growth of the bacteria was 6.5. After incubation the pH in culture media was neutralized. Among the various kinds of carbon sources, sucrose, glucose mannitol, but organic acids were not utilized effectively as nutrients. After incubation the pH turned acidic. Alanine as organic nitrogen sources nad ammonium sulfate as inorganic nitrogen promoted the growth, but L-valine and sodium nitrite were the least effective. Ferric chloride 1.0mg/dl and ferrous sulfate 100mg/dl were the most effective as micrometallic sources. Control and boric acid were the least effective. New synthetic medium based on the above results was follows ; Alanine 1.0g, $KH_2PO_4$ 1.0g, sucrose 30.0g, $MgSo_4$ $7H_2$O 0.5g, $FeCl_36H_2$O 1.0mg thiamine 200.gamma.g, and distilled water 1000ml, pH6.5.

• 대한수학회지
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• 제38권4호
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• pp.723-761
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• 2001

The objects of this paper are to formulated a model for the transport of a chain of radioactive waste products in a fractured porous medium, to devise an effective and efficient numerical method for approximating the solution of the model, and to demonstrated the convergence of the numerical method. The formulation begins from a model in an unfractured (single porosity) medium, passes through a double porosity model in a fractured medium, and ends with a modified single porosity model that takes the relevant time scales of the flow and the nuclear decay.

• 한국약용작물학회지
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• 제12권5호
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• pp.372-377
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• 2004

Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

• 미생물과산업
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• 제26권1호
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• pp.18-30
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• 2000

질소 고정능이 있는 Nostoc pruniforme를 재료로, 3종의 유기물, glucose, oxalic, 및 ethanol을 N-free medium과 NO$^{[-993]}$ riched medium에 처리해 주었을 때 다음과 같은 효과를 얻었다. 1)N-free medium과 NO$^{[-993]}$ riched medium에서 위의 유기물 모두가 질소 고정과정의 energy원으로서 또는 substrate로서의 유효한 결과를 얻었다. 3)oxalic acid를 처리한 실험에서는NO$^{[-993]}$ richedmedium의 경우 고농도 처리구에서 질소고정량이 많았고 N-free medium은 그 반대의 결과였다. 4)비교구인 control의 질소 고정량은 일예를 제회하고는 전반적으로 고농도 처리구와 저농도 처리구의 중간에 위치했다.

• 한국전산구조공학회논문집
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• 제17권4호
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• pp.451-458
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• 2004

본 연구는 동결토의 동결심도 및 매설된 파이프라인의 온도분포를 예측하기 위하여 유효열용량 개념 반영한 수치해석 모델의 개발에 초점이 맞추어져 있다. 이를 위하여 저자는 사용코드인 ANAQUS를 활용하여 파이프라인이 매설된 폐쇄형 시스템의 화강 동결토에 대하여 비정상 열전달 수치해석을 수행하였다. 제안된 수치해석 모델은 Frozen Fringe에서 간극수의 상변화 효과가 반영되었다. 제안된 수치해석 모델과 실내 실험으로부터 얻어진 결과들을 비교함으로써 유효열용량 모델의 적용성을 검증하였다.

• 식물조직배양학회지
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• 제22권4호
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• pp.223-227
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• 1995

네오게레리아의 대량번식을 위해 미숙화기와 액아를 배양하였으며, 배양에 적합한 재료 채취시기, 생장조절제 종류와 농도 및 배양방법이 부정아 형성 및 생장에 미치는 영향을 조사한 결과, 미숙화기는 화아분화 4주후에, 액아는 화아 분화 7주후에서 절취하여 배양하는 것이 부정아 형성률이 가장 높았다. 부정아 형성에 적합한 생장조절제 종류와 농도는 MS + BA 1 mg/L + BA 0.5 mg/L이었다. 고체배지가 액체배지보다 부정아 형성 및 생장에 더 효과적이었다. 발근에 적합한 생장조절제 종류와 농도는 NAA 1 mg/L이었다. 액체배지가 고체배지보다 신초의 발근 및 생장에 더 효과적이었다.

• Current Optics and Photonics
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• 제2권5호
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• pp.482-487
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• 2018

We investigate the radiation characteristics of radially polarized light and azimuthally polarized light through plasmonic subwavelength-scale annular slit (PSAS) structures, by means of both theoretical and numerical methods. Effective-medium theory was utilized to analyze the characteristics of PSAS structures, and the corresponding results showed that PSAS structures can function as a metallic medium for azimuthally polarized light, or as a low-loss dielectric medium for radially polarized light. Numerical calculations based on the finite-element method were also performed, to verify the theoretical analyses. It turned out that the numerical results supported the theoretical results. Moreover, we exploited the PSAS structures in novel nanophotonic elements with dual functionalities that could selectively focus or pass/block incident light, depending on its polarization state. For example, if PSAS structures were implemented in the dielectric region of a metallic Fresnel zone plate, the modified zone plate could function as a blocking element to azimuthally polarized light, yet as a focusing element to radially polarized light. On the contrary, if PSAS structures were implemented in the metallic region of a metallic Fresnel zone plate (i.e. the inverted form of the former), it could function as a focusing element to azimuthally polarized light, yet as a simple transparent element to radially polarized light.