• 한국작물학회지
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• 제52권4호
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• pp.403-410
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• 2007

A field experiment was conducted to investigate effects of application time and rate of biofertilizer alone and in combination with chemical NPK fertilizer on growth, yield and quality of rice. The biofertilizer used composted food waste as substrate and added with effective microorganism. The treatments included recommended NPK fertilizer(RF, $11-5.5-4.8kg\;10a^{-1}$), half recommended NPK fertilizer(HRF, $5.5-2.8-2.4kg\;10a^{-1}$), half recommended NPK fertilizer plus $250kg\;10a^{-1}$ biofertilizer(HRF+Bio 250) and $500kg\;10a^{-1}$ biofertilizer(HRF+Bio 500). The biofertilizer treatments were applied at 0, 5 and 10 days before transplanting(DBT). Grain yield of HRF+Bio 250 at 5 DBT($648.4kg\;10a^{-1}$) was statistically similar to the highest obtained in the RF($654.1kg\;10a^{-1}$). Tiller numbers at HRF plus biofertilizer treatments were already high during the maximum tillering stage, and were similar with that of the RF and higher than that of the HRF during heading stage. Likewise, ripening ratio at HRF plus biofertilizer treatments was similar with that of the RF and higher than that of the HRF. Furthermore, all the biofertilizer treatments improved protein content but reduced the amylose content and palatability compared to treatments with chemical NPK fertilizer alone. Thus, HRF+Bio 250 at 5 DBT can be used to save 50% chemical NPK fertilizer and at the same time obtain an improved rice grain yield and quality.

• 전자공학회논문지
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• 제49권11호
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• pp.199-204
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• 2012

지지구 온난화 등과 관련하여 에너지 개발과 절약에 대한 연구가 활발하다. 본 연구에서는 전체 전기에너지 사용의 19%를 차지하는 조명기구 소비전력의 효율적인 절감과 수명연장을 위해 주파수변조와 펄스폭 변조를 혼용한 전류제어 방식의 새로운 형태인 하프브리지형 전원장치를 제안한다. 제안한 전원장치가 갖는 우수성과 안정성에 대해 입증하고 그 특성을 분석 하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.159-159
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• 2022

Nitric oxide (NO) is a versatile signaling molecule, which is not only involved in plant growth and development but also regulates biological processes in response to biotic and abiotic stresses. Exogenous application of NO regulates the endogenous level of nitric oxide in response to stress conditions and therefore, NO donors are frequently used for stress alleviation. However, NO has very short half-life along with high reactivity. Therefore, conventional NO donors are often disadvantageous due to the relative instability of NO. On the contrary, development of NO releasing nanoparticles is a potential technique for enhancing the availability of NO in plants. Therefore, our aim was to synthesize such potential NO releasing nanoparticles which may be useful for application in agriculture. We have prepared Chitosan encapsulated S-nitrosoglutathione nanoparticles (GSNONP) and tried it with different concentrations for basic research in Arabidopsis thaliana. Our results suggest that lower concentration of this nanoparticle is highly effective for better growth of plants whereas higher concentration produces toxicity that leads to plant death. We observed better growth of Arabidopsis thaliana at 1µM concentration of the GSNONP compared to free GSNO.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.225-225
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• 2022

Rice is a major food crop consumed by approximately half of the world's population. Heading date is one of the major agronomic traits and has a wide impact on the productivity and quality of rice. Recently, shortening the growth period of rice through modulating heading date has been emphasized as one of the most effective strategies for reducing methane emissions from paddy fields. OsPRR37, a major heading date gene in rice, represses flowering under both short-day and long-day conditions. Plants carrying the loss-of-function alleles of OsPRR37 have been reported to flower approximately seven days and 20 days earlier than those carrying the functional alleles in short day and long day conditions, respectively. In this study, we investigated the nucleotide sequence variation existing in the exonic regions of OsPRR37 and catalogued the allelic distribution in 208 Korean rice cultivars. We used four sets of primers for amplifying and sequencing the eight exons of OsPRR37. As a result, two types of loss-of-function alleles and four types of functional alleles were found in 208 Korean rice cultivars. Interestingly, only three cultivars (Jinbuolbyeo, Jinseolchal, and Mimyeon) carried loss-of-function alleles while 205 carried functional alleles, indicating that OsPRR37 loss-of-function alleles have been used very rarely in Korean rice breeding programs. To generate useful information for the development of early-maturing rice cultivars, our future work will focus on analyzing the effect of different OsPRR37 alleles on heading date and other major agronomic traits.

• Archives of Pharmacal Research
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• 제26권3호
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• pp.224-231
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• 2003

The anti-thrombotic effects of LB30057, a direct thrombin inhibitor, were evaluated with in vivo rat and dog thrombosis models. In rats, 1 mg/kg of LB30057 inhibited half of the clot formations in the inferior vena cava at 5 minutes after intravenous application. When measured at 2 hours after oral application, 100 mg/kg prevented approximately half of the clot formations in the inferior vena cava and 50 mg/kg prolonged the mean occlusion time from $15.6{\pm}1.3$ minutes to $47.2{\pm}8.3$ minutes in the carotid artery. In dogs, the formation of thrombus in the jugular vein was reduced to half at a dose range of 20-30 mg/kg at 6 hours after oral application. In addition, the LB30057 dosage required to reduce venous clot formation by approximately 80-90% in dogs was only about 10% of that required for the same reduction in rats. This is probably due to the variation in its time-dependent blood concentration profiles in each species; for example, the plasma half-life of LB71350 in dogs was longer than that in rats ($153.0{\pm}3.0$ vs. $129.7{\pm}12.7$ min at 30 mg/kg, i.v., respectively). AUG, $T_{max},{\;}G_{max}$, and BA in dogs were 59, 8.9, 9.17, and 13.3 times higher than those in rats at oral 30 mg/kg, respectively. Taken together, these results suggest that LB30057 administered orally is effective in the prevention of arterial and venous thrombosis in rats and dogs. It therefore represents a good lead compound for investigations to discover a new, orally available, therapeutic agent for treating thrombotic diseases.

• 원예과학기술지
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• 제28권5호
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• pp.790-795
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• 2010

본 연구는 절화 아이리스 '블루매직'의 수명에 미치는 cycloheximide(CHI)와 보존용액의 효과를 알기 위해 실시하였다. 아이리스의 절화수명은 증류수구에 비해 3% sucrose (S) + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA 또는 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI, 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI 처리구에서 현저히 길었다. $200mg{\cdot}L^{-1}$ HQS를 보존용액에 첨가했을 때 나타나는 줄기 기부의 쪼그라듬 현상은 $100mg{\cdot}L^{-1}$ HQS 처리 시 완전히 없어졌다. 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA 처리는 절화수명, 생체중, 수분균형, 개화에 가장 효과적이었다. 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI, 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI 보존용액 처리시 용액흡수량과 증산량은 낮았으나 수분균형은 높았다. 반개화 상태에서 수확한 아이리스에 CHI 처리는 노화를 지연시켰고 절화수명도 현저히 증가시켰다. 봉오리 단계의 아이리스에 CHI를 함유한 보존용액을 처리하면 개화가 잘 이루어지지 않으나, 꽃잎이 벌어진 상태에서 CHI를 함유한 보존용액 처리를 하면 개화가 완전하게 이루어졌다.

• 생명과학회지
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• 제8권5호
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• pp.520-525
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• 1998

한란(Cymbidium kanran)의 급속증식을 위한 식물생장 조절물질과 항산화제의 처리결과, 2.5 g/1 gelrite가 생장과 경제적인 면에서 우수하였는데, 9 g/1 시약한천보다 적은 약 28% 정도의 양과 절반 정도의 가격으로 우수한 결과를 나타났다. 식용한천은 시약한천에 비해 절반 정도의 가격으로 거의 비슷한 생육을 보였다. BA와 NAA의 농도가 높을수록 배지의 갈변도가 심하였으며, 식물생장조절물질의 작용을 억제하였다. polyvinylpyrrolidone (PVP, M.W. 40,000)이 ascorbic acid, aspartic acid, rutin 보다 우수 항산화제인 것으로 나타났다. 식물생장조절물질 처리에서는 0.1-1.0 mg/1 BA와 0.1 mg기 NAA, 1 g/1 PVP 처리구가 가장 좋은 생육을 보였다. 따라서 한란의 경우 급속한 개체 증식을 위해서는 MS 기본배지에 2.5 g기 gelrite를 불활성 지지물로 사용하고, 0.1-1.0 mg/1 BA 수준에 0.1 mg/1 NAA를 처리하고, 1 g/1 PVP 첨가를 통해 배지의 산화를 억제하여 배양기간을 연장하고, 식물생장조절물질의 역할이 정상적으로 작용하도록 하여 적은 양의 식물생장조절물질의 사용으로 강건한 묘를 확보할 수 있는 것으로 나타났다.

• Molecules and Cells
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• 제42권12호
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• pp.869-883
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• 2019

Interleukin (IL)-15 is an essential immune-modulator with high potential for use in cancer treatment. Natural IL-15 has a low biological potency because of its short half-life and difficulties in mass-production. IL-15Rα, a member of the IL-15 receptor complex, is famous for its high affinity to IL-15 and its ability to lengthen the half-life of IL-15. We have double-transfected IL-15 and its truncated receptor IL-15Rα into CT26 colon cancer cells to target them for intracellular assembly. The secreted IL-15:IL-15Rα complexes were confirmed in ELISA and Co-IP experiments. IL-15:IL-15Rα secreting clones showed a higher anti-tumor effect than IL-15 secreting clones. Furthermore, we also evaluated the vaccine and therapeutic efficacy of the whole cancer-cell vaccine using mitomycin C (MMC)-treated IL-15:IL-15Rα secreting CT26 clones. Three sets of experiments were evaluated; (1) therapeutics, (2) vaccination, and (3) long-term protection. Wild-type CT26-bearing mice treated with a single dose of MMC-inactivated secreted IL-15:IL-15Rα clones prolonged survival compared to the control group. Survival of MMC-inactivated IL-15:IL-15Rα clone-vaccinated mice (without any further adjuvant) exceeded up to 100%. This protection effect even lasted for at least three months after the immunization. Secreted IL-15:IL-15Rα clones challenging trigger anti-tumor response via CD4⁺ T, CD8⁺ T, and natural killer (NK) cell-dependent cytotoxicity. Our result suggested that cell-based vaccine secreting IL-15:IL-15Rα, may offer the new tools for immunotherapy to treat cancer.

• 한국환경농학회지
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• 제30권2호
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• pp.196-201
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• 2011

BACKGROUND: Bioavailability enhancement by solubilization of lipophilic drugs in nano-emulsion has been reported and it may be useful in pharmaceutical and nutraceutical products. This study was performed to compare in vivo bioavailability of nano-emulsion formulation with that of the general product as control. METHODS AND RESULTS: The pharmacokinetics assessment of Vitamin A, D and E complex of nanoemulsion formulation (LaVita), in comparison to the general product, was performed in the male rat plasma by a single oral dose at 20 mL/kg body weight (n=3/group). For nano-emulsion formulation (LaVita), $C_{max}$ of vitamin A and E in plasma were much higher and the area under the curve (AUC) of vitamin A, D and E were 14-63% higher, and the half-life of vitamin E was 2-fold longer than the general product. According to statistical analysis, each $C_{max}$ of vitamin A, D & E was significantly higher (p<0.01, 0.05 and 0.01, respectively) than that of general product. Half-life of vitamin A was significantly higher (p<0.01) and AUC of vitamin A and D were also significantly higher than the general product. CONCLUSION(s): Considering significant increment of $C_{max}$ and AUC, LaVita made of nano-emulsion could be more effective the absorption rate and extent for bioavailability of vitamin A, D & E than those of general product.

• 대한임상독성학회지
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• 제17권2호
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• pp.102-107
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• 2019

Purpose: Acute carbon monoxide (CO) poisoning is one of the most common types of poisoning and a major health problem worldwide. Treatment options are limited to normobaric oxygen therapy, administered using a non-rebreather face mask or hyperbaric oxygen. Compared to conventional oxygen therapy, high-flow nasal cannula oxygen (HFNC) creates a positive pressure effect through high-flow rates. The purpose of this human pilot study is to determine the effects of HFNC on the rate of CO clearance from the blood, in patients with mild to moderate CO poisoning. Methods: CO-poisoned patients were administered 100% oxygen from HFNC (flow of 60 L/min). The fraction of COHb (fCOHb) was measured at 30-min intervals until it decreased to under 10%, and the half-life time of fCOHb (fCOHb t_1/2) was subsequently determined. Results: At the time of ED arrival, a total of 10 patients had fCOHb levels ≥10%, with 4 patients ranging between 10% and 50%. The mean rate of fCOHb elimination patterns exhibits logarithmic growth curves that initially increase quickly with time (HFNC equation, Y=0.3388*X+11.67). The mean fCOHbt_1/2 in the HFNC group was determined to be 48.5±12.4 minutes. Conclusion: In patients with mild to moderate CO poisoning, oxygen delivered via high flow nasal cannula is a safe and comfortable method to treat acute CO toxicity, and is effective in reducing the COHb half-life. Our results indicate HFNC to be a promising alternative method of delivering oxygen for CO toxicity. Validating the effectiveness of this method will require larger studies with clinical outcomes.