• Journal of Life Science
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• v.25 no.2
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• pp.180-188
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• 2015

The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.

• Culinary science and hospitality research
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• v.21 no.4
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• pp.192-206
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• 2015

A study was conducted on dietitians in the Gyeonggido area to analyze the effect of purchase decision and attitude related factors regarding environmental-friendly agricultural products. The results of the factor analysis, correlation analysis and a multiple regression analysis are as follows. Many dieticians prefer environmental-friendly agricultural products, but they are reluctant to pay more money to purchase them. It was found that purchasing decision factors were 'loyalty' at 0.920, 'perceived quality' at 0.791, 'awareness' at 0.862, and 'brand' at 0.801. To conclude, the research result shows a statistically very close and positive correlation between the dieticians' attitude on environmental-friendly agricultural products and purchase decision. Other factors of quality(r=.296, p<.01), brand(r=.350, p<.01), loyalty(r=.558, p<.01), and awareness (r=.496, p<.01) acted as the most meaningful and significant variables. In short, of the dieticians surveyed in Gyeonggido province, the more favorably disposed to organic products, the more likely they are to purchase them. Accordingly, it is necessary for dieticians to be educated so that they may have greater awareness of environmental-friendly agricultural products. Furthermore, there is a need for more follow-up studies in order to gain an understanding of how to improve dieticians' attitudes on using organic products.

• Journal of Life Science
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• v.31 no.4
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• pp.377-384
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• 2021

Researching the soil fungal community is important to understand the interaction between fungi and living plants. However, too few studies have examined the soil fungal community and their interactions with plants. Rhododendron mucronulatum, commonly known as Korean rosebay, is an important forest resource that has aesthetic, ecological, and potential pharmacological values. We used a pyrosequencing method to analyze the characteristics of fungal communities from R. mucronulatum soil samples from Biseul mountain county park, which is one of the famous places for large R. mucronulatum colonies in South Korea. We collected soil core samples in February and August at three sites in the Biseul Mountain County Park, taking into consideration the regional and seasonal conditions. We obtained 454,157 validated reads after pyrosequencing all six samples. The fungal communities from the first observation spot in August had the richest species diversity among the samples. Basidiomycota, Ascomycota, and Mortierellomycota were major phyla in the samples. Agaricales_f, Mortierellaceae, and Clavariaceae were major families in the samples. The genus Mortierella was the most dominant in all six samples. Overall, 19 genera could be associated with R. mucronulatum. Sample 1 had 109 genera in sample 1, sample 2 had 111 genera, and sample 3 had 112 genera that were uniquely identified. The samples collected in August had 28 identified genera, that existed only in summer samples, indicating a weather effect. This study can be used as basic research to understand the relationship between soil fungi and plants.

• Journal of Mushroom
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• v.18 no.4
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• pp.339-343
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• 2020

This study aimed to optimize total nitrogen (TN) content in the medium and the growth temperature to improve the shelf life of Pleurotus eryngii Keneutari No. 2. We investigated the growth characteristics and changes in the O2/CO2 content in polypropylene film and performed sensory evaluations during cold storage under 1.5%, 1.8%, 2.1%, and 2.4% TN and at temperatures of 9℃, 12℃, and 15℃. The diameter and weight of the fruit bodies tended to increase with increasing nitrogen content, regardless of the growth temperature, and the highest weight per bottle (149.5 g) was obtained at 12℃ and 2.4% TN. Regardless of the nitrogen content, leaf color deepened with decreasing temperature. The gas concentration in the film, which changes depending on storage time, was maintained for the longest period at 9℃ and 2.1% TN. The sensory evaluation revealed no difference between the different nitrogen conditions tested, and the odor, discoloration, and decay were similar at 15℃ and 9℃ until day 28 of storage.

• Environmental and Resource Economics Review
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• v.31 no.2
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• pp.233-260
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• 2022

In this paper, we analyzed how oil price fluctuations affect stock price by industry using the non-parametric quantile causality test method. We used weekly data of WTI spot price, KOSPI index, and 22 industrial stock indices from January 1998 to April 2021. The empirical results show that the effect of changes in oil prices on the KOSPI index was not significant, which can be attributed to mixed responses of diverse stock prices in several industries included in the KOSPI index. Looking at the stock price response to oil price by industry, the 9 of 18 industries, including Cloth, Paper, and Medicine show a causality with oil prices, while 9 industries, including Food, Chemical, and Non-metal do not show a causal relationship. Four industries including Medicine and Communication (0.45~0.85), Cloth (0.15~0.45), and Construction (0.5~0.6) show causality with oil prices more than three quantiles consecutively. However, the quantiles in which causality appeared were different for each industry. From the result, we find that the effects of oil price on the stock prices differ significantly by industry, and even in one industry, and the response to oil price changes is different depending on the market situation. This suggests that the government's macroeconomic policies, such as industrial and employment policies, should be performed in consideration of the differences in the effects of oil price fluctuations by industry and market conditions. It also shows that investors have to rebalance their portfolio by industry when oil prices fluctuate.

• Clean Technology
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• v.29 no.2
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• pp.95-101
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• 2023

An experiment was conducted to produce vaterite-type precipitated calcium carbonate from waste oyster shells in order to use them as recyclable resources. Calcined oyster shells containing calcium oxide as their main component were prepared at a temperature of 800℃ for 24 h. The oyster shells were dissolved in nitric acid or hydrochloric acid solution to make 0.1 M calcium nitrate or calcium chloride aqueous solution, and a carbonation reaction was performed using a 0.1 M sodium carbonate aqueous solution under various experimental conditions, which included varying the amount of aspatic acid additive, the amount of NH₄OH added, the reaction time, the reaction temperature, the stirring speed, and the type of dissolved acid. The XRD, SEM, and size distributions were analyzed and the vaterite content was calculated. Spherical precipitated calcium carbonate with a vaterite content of 95.9% was synthesized by adding 0.1 mol aspatic acid/1 mol CaO and 2 cm³ of NH₄OH, and reacting for 1 h at 25℃ while stirring at 600 rpm. The average particle diameter was found to be 12.11 ㎛. Calcium carbonate contatining high vaterite is used as high value added calcium carbonate for medical, food, inke additiver, etc.

• Journal of the Korean Applied Science and Technology
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• v.40 no.4
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• pp.710-723
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• 2023

The purpose of this study is to investigate the effect on the revisit intention and recommendation intention on the golf course selection attribute factors of Chinese golf course users using IPA. In order to carry out this study, a survey was conducted on 388 Chinese golfers, data processing and IPA analysis were conducted, and the following conclusions were obtained. First, the importance of the selection attribute was the highest in employee etiquette, and the price of restaurants and food and beverage was the highest in satisfaction. Second, as a result of the IPA matrix analysis, there were 8 selection attributes for continuous maintenance, 14 for concentration improvement, 5 for low priority, and 3 for excessive effort avoidance. Third, cost, accessibility, course facilities, auxiliary facilities, caddy expertise, and satisfaction with user management were found to affect both revisit and recommendation intentions. Since this study was limited to Chinese golfers, there may be differences in comparing the results with previous studies conducted in other countries on golf culture. Therefore, studies considering cultural diversity should be conducted in the future.

• KSBB Journal
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• v.6 no.3
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• pp.309-319
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• 1991

A continuous stirred tank membrane reactor(CSTMR ) was developed and optimized for the production of cod skin gelatin hydrolyzates using endo-protease Alcalase. A experimental design methodology was used to optimize the four performance variables: enzyme concentration, substrate concentration, permeate flux and reactor volume. All four variables studied had an effect on substrate conversion, with enzyme and substrate concentrations being predominant. Conversion increased with the increase in enzyme concentration, with the decrease in substrate concentration, at high volumes and low flux. A strong interaction was observed between enzyme and substrate concentrations and smaller interactions between enzyme and flux and substrate and flux. The optimum operating conditions for the CSTMR process for an initial substrate concentration for 10% were $50^{\circ}C$, pH 8, flux 7.3ml/min, residence time 82 min, and Alcalase to substrate ratio 0.02(w/w). A gradual decay in reactor activity during 8 hrs was 2.1% conversion/hr. Enzyme leakage through the 10, 000 MWCO membrane was 16% at $50^{\circ}C$ and 12% at $35^{\circ}C$, 6hrs. However, there was no apparent correlation between enayme leakage and substrate conversion. The Km value for the CSTMR was 20 times higher than the batch reactor. The productivity(expressed as mg product/mg enzyme) of the CSTMR was more than six fold higher than the batch at $50^{\circ}C$. The hydrolyzate was non-bitter.

• Culinary science and hospitality research
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• v.18 no.2
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• pp.91-105
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• 2012

This study aims to analyze the effects of satisfaction on behavioral intentions depending on brand equity based on the consumers who visited specialty coffee shops in Busan. The self-administered method was used for empirical analysis and 295 copies (89%) out of 300 copies of questionnaires were used for analysis. For data processing, the SPSS WIN Version 15.0 statistical package program was used and the frequency analysis, reliability analysis, factor analysis were conducted so the hypothesis was verified through the regression analysis. As a result, for the hypothesis that "Brand equity(perceived quality, brand awareness, brand image) will have a significant impact on satisfaction," perceived quality, brand awareness, and brand image had a significant impact on satisfaction. Satisfaction was shown to have a significant impact on behavioral intentions for the hypothesis that "Satisfaction will have a significant impact on behavioral intentions" For the hypothesis that "Brand equity(perceived quality, brand awareness, brand image) will have a significant impact on behavior intentions," perceived quality and brand awareness had a significant impact on behavioral intentions while brand image didn't. Based on the results of this study, companies should be systematically managed to prepare strategies to remind consumers of their brands and focus on to strengthen brand equity effectively in order to build a competitive brand.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.233-241
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• 2015

Kimchi cabbage 'Choon Gwang' cultivar that was grown in highlands in Gangneung was subjected to predrying, room cooling, and forced air cooling, and then packed with/without 0.02 mm HDPE film to investigate the effect of postharvest treatment on quality characteristics during 8 weeks storage at $2^{\circ}C$ (RH $90{\pm}5%$). Weight loss in forced air cooling and room cooling was lower than 3-4% with 0.02 mm HDPE film liner treatment during storage. However, it was only below 10% in room cooling without liner treatment and forced air cooling without liner treatment led to the highest weight loss, above 15%. Conversely, the control had lower weight loss than the others. SSC was $2-4^{\circ}brix$ for all treatments and there was no difference between postharvest treatments and liner treatments. Color index and firmness both showed no differences with/without 0.02 mm HDPE film and postharvest treatments. In sensory evaluation, forced air cooling with liner treatment was effective, with the highest score, especially in appearance and crispness. After 6 weeks, control kimchi cabbage without liner treatment was damaged seriously in appearance and the internal color had changed to brown. Room cooling and predrying with liner treatment changed the start of internal browning to after 8 weeks storage.