• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.25-30
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• 2011

Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF-${\kappa}B$ is a transcription factor involved in many biological activities. Expression and activity of NF-${\kappa}B$ increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF-${\kappa}B$ protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF-${\kappa}B$ in the human Nanog promoter and postulated that NF-${\kappa}B$ protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF-${\kappa}B$ upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF-${\kappa}B$ binding site suggested involvement of NF-${\kappa}B$ in the negative regulation of the promoter, site-directed mutation of NF-${\kappa}B$ binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF-${\kappa}B$ with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF-${\kappa}B$ protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.

• Asian-Australasian Journal of Animal Sciences
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• 제20권2호
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• pp.184-193
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• 2007

In mammalian ovary, steroidogenic acute regulatory (StAR) protein mediates the true rate-limiting step of transport of cholesterol from outer to inner mitochondrial membrane. Appropriate expression of StAR gene represents an indispensable component of steroidogenesis and its regulation has been found to be species specific. However, limited information is available regarding StAR gene expression during estrous cycle in buffalo ovary. In the present study, expression, localization and hormonal regulation of StAR mRNA were analyzed by semi-quantitative RT-PCR in buffalo ovary and partial cDNA was cloned. Total RNA was isolated from whole follicles of different sizes, granulosa cells from different size follicles and postovulatory structures like corpus luteum and Corpus albicans. Semi-quantitative RT-PCR analyses showed StAR mRNA expression in the postovulatory structure, corpus luteum. No StAR mRNA was detected in total RNA isolated from whole follicles of different size including the preovulatory follicle (>9 mm in diameter). However, granulosa cells isolated from preovulatory follicles showed the moderate expression of StAR mRNA. To assess the hormonal regulation of StAR mRNA, primary culture of buffalo granulosa cells were treated with FSH (100 ng/ml) alone or along with IGF-I (100 ng/ml) for 12 to 18 h. The abundance of StAR mRNA increased in cells treated with FSH alone or FSH with IGF-I. However, effect of FSH with IGF-I on mRNA expression was found highly significant (p<0.01). In conclusion, differential expression of StAR messages was observed during estrous cycle in buffalo ovary. Also, there was a synergistic action of IGF-I on FSH stimulation of StAR gene.

• 아태비즈니스연구
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• 제15권1호
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• pp.239-256
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• 2024

Purpose - The purpose of this study was to examine the main determinants of the gap between housing demand and house affordability. Design/methodology/approach - This study used the micro-level data of 60,043 households from Korea Housing-Finance Corporation by covering the period 2011 to 2022. Findings - First, the trend of general housing demand showed a higher figure in the future demand than in current demand. And such a tendency showed in all types of households, a relative young, low income, and single households. In the case of current housing demand, it has increased by 2022 from the beginning of 2013, while the future demand has rapidly increased from 2020. Second, although the house affordability showed a higher figure in current housing demand by 2019, its trend changed to be higher in future housing demand from 2020 by a rapid decreasing affordbility in current demand. In the case of young householders, the current house affordability was higher than that of future. The figure of low income householders was below 1 point in both periods, and house affordability of single householders showed a similar level in both periods. which showed over 1 point. Third, financial regulation on housing markets induced th widening of the gap between housing demand and house affordability, and such a trend is much atronger in the future(potential) gap of demand and affordability. More specifically, the strengthen financial regulation leaded to the widening of the gap in all types of households, a relative young, low income, and single households. Research implications or Originality - The effect of financial regulation is necessary to consider under the features of each households.

• Archives of Pharmacal Research
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• 제10권4호
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• pp.212-218
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• 1987

Intact brain cell aggregates were dissociated from adult rat brains without cerebellum using a sieving technique. This proparation was used to elucidate the binding characteristics of agonist to muscarinic acetylcholine receptors (mAchR) in brain. Incubation of cells with carbamylcholine (carbachol) was shown agonist-induced receptor down-regulation depending on the concentration of agonist, not depending on the incubation time. This effect of carbachol was due to a reduction in the maximal binding capacity ($B_{max}$) to the mAchR without decreasing the affinity of the remaining receptors in incubation at 37.deg.C but was not apparent inincubation at $15^{\circ}}C$In addition, it was abolished when the receptors were blocked by atropine. The decline in ($^3H$)N-methylscopolamine (($^3H$)NMS) binding induced by agonist was reflected as a significant reduction in the receptor density with no change in receptor affinity, suggesting that 'true' receptor down-regulation takes place. Moreover, when the receptors were labeled with the lipophilic antagonist ($^3H$) quinuclidinyl benzilate (($^3H$) QNB) insted of the hydrophilic ligand ($^3H$)NMS, the magnitude of the observed receptor down-regulation was significantly lower in case of the former than the latter. This suggested that exposure of intact brain cells to muscarinic agonists might induce a slight degree of accumulation of receptors in intracellular sites before the receptors are actually degraded.

• 한국항해항만학회지
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• 제32권10호
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• pp.785-791
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• 2008

최근 선박을 이용한 화물운송의 증대, 해양관광 및 레저 활동을 위한 연안 여객선의 운항 증가, 그리고 해양생태계 파괴와 사회적 문제로 확산된 서해안 기름유출 사고 등 해양에 대한 관심이 갈수록 고조되고 있는 상황에서 선박의 안전운항은 필수적인 요건이다. 이러한 상황에서 현재 운용되고 있는 선박의 출항통제기준에 대한 보다 정량적이고 객관적인 검토가 시급한 실정이다. 본 논문에서는 선박 운항 시 안전운항과 가장 밀접한 관련을 갖는 파랑을 분석하여, 우리나라 연안항로에 취항하고 있는 대표적인 선형의 여객선들을 대상으로 해상상태 및 선형에 따라 내항성능지표를 이용하여 선박의 항해안전성을 평가하였다. 이러한 결과는 선박출항통제 기준의 개선과 연안 여객선의 안전운항에 큰 도움이 될 것으로 판단된다.

• Genes and Genomics
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• 제40권12호
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• pp.1331-1338
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• 2018

Although there have been plenty of dominance deviation analysis, few studies have dealt with multiple phenotypes. Because researchers focused on multiple phenotypes (final weight and backfat thickness) of Landrace pigs, the classification of the genes was possible. With genome-wide association studies (GWASs), we analyzed the additive and dominance effects of the single nucleotide polymorphisms (SNPs). The classification of the pig genes into four categories (overdominance in final weight, overdominance in backfat thickness and overdominance in final weight, underdominance in backfat thickness, etc.) can enable us not only to analyze each phenotype's dominant effects, but also to illustrate the gene ontology (GO) analysis with different aspects. We aimed to determine the additive and dominant effect in backfat thickness and final weight and performed GO analysis. Using additive model and dominance deviation analysis in GWASs, Landrace pigs' overdominant and underdominant SNP effects in final weight and backfat thickness were surveyed. Then through GO analysis, we investigated the genes that were classified in the GWASs. The major GO terms of the underdominant effects in final weight and overdominant effects in backfat thickness were ion transport with the SLC8A3, KCNJ16, P2RX7 and TRPC3 genes. Interestingly, the major GO terms in the underdominant effects in the final weight and the underdominant effects in the backfat thickness were the regulation of ion transport with the STAC, GCK, TRPC6, UBASH3B, CAMK2D, CACNG4 and SCN4B genes. These results demonstrate that ion transport and ion transport regulation genes have distinct dominant effects. Through GWASs using the mode of linear additive model and dominance deviation, overdominant effects and underdominant effects in backfat thickness was contrary to each other in GO terms (ion transport and ion transport regulation, respectively). Additionally, because ion transport and ion transport regulation genes are associative with adipose tissue accumulation, we could infer that these two groups of genes had to do with unique fat accumulation mechanisms in Landrace pigs.

• 우울조울병
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• 제9권2호
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• pp.78-86
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• 2011

Objectives : The aim of this study was to investigate relationship between perceived stress level, cognitive emotion regulation (CER) strategy and hope in group with high depressive symptom and higher-level perceived stress (H-H) and group with high depressive symptom and lower-level perceived stress (H-L) in patients with depression. Method : A total of 85 patients (over score of 16 by Beck depression Inventory; BDI) were surveyed with Cognitive emotion regulation questionnaire (CERQ), Perceived stress scale (PSS), and The state hope scale (SHS). Mean scores of CERQ and SHS were compared between relatively higher perceived stress and lower perceived stress groups. Correlation analysis and multiple linear regression analyses were performed to identify the effect of BDI, PSS and CER strategy on SHS in two groups. Results : In ANOVA, the level of hope and maladaptive CERQ score proved to be significantly lower among the H-H group than among the H-L group, while adaptive CERQ scores were not. In Regression analysis, the effective CER strategy in SHS were 'Refocus on planning' in H-H group, while it was 'Acceptance' CER strategy in H-L group. The final regression model explained 36% of the variance of SHS in H-H group and explained 21% of SHS in H-L group. Conclusion : These findings suggest that 'Refocus on planning' and 'Acceptance' cognitive emotion strategy are helpful in promotion of state hope on depression. Especially, 'Refocus on planning' strategy is more effective in high depressive symptom and high-perceived stress level, while 'Acceptance' strategy help to promote hope in high depressive symptom and low-perceived stress level in patients with depression.

• 정신신체의학
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• 제20권2호
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• pp.135-138
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• 2012

기능성 위장관 장애(functional gastrointestinal disorder, FGID)의 생물학적 병태생리는 아직 명확하지 않은 실정이나 최근에 뇌-장관 축(brain-gut axis, BGA) 모델이 각광받고 있다. BGA 모델은 중추신경계(CNS)와 장관신경계(ENS)가 신경계, 신경내분비계, 신경면역계 등으로 밀접하게 연계되어 양방향성으로 항상성을 유지한다는 이론이다. 또한 BGA는 FGID에 대한 항우울제 효과의 이론적 근거를 제공한다. 저자들은 세로토닌 노르에프네프린 재흡수 차단제인 duloxetine이 목 이물감, 우울감을 보이는 환자와 상부 위장관 팽만감, 건강염려증적 불안을 보이는 환자에서 위장관 증상 및 정신의학적 증상에 효과적이었음을 경험하였다. 이에 두 증례보고를 통하여 BGA에 대해서 간략히 검토하고 duloxetine의 효과를 BGA의 측면에서 고찰하였다. 이 두 증례에서 duloxetine의 장관에서 뇌로의 영향(bottom-up regulation)으로는 세로토닌 수용체, 노르에피네프린 수용체 그리고 부신피질자극호르몬방출인자(CRF) 길항효과를 통해서 내장과민성(visceral hypersensitivity) 및 정신의학적 증상을 호전시키고, 뇌에서 장관으로의 영향(top-down regulation)으로는 우울과 불안을 호전시킴으로 위장관 증상에 영향을 미친 것으로 추정해 볼 수 있다.

• Journal of Radiation Protection and Research
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• 제49권1호
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• pp.19-28
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• 2024

Background: This study aims to analyze radiation safety management and regulatory perceptions, focusing on companies that must report radiation sources. The intent is to reduce the gap between regulation measures and addressing real concerns while improving practical safety management measures and regulations for all stakeholders. Materials and Methods: Radiation safety officers at a total of 244 reporting companies using radiation generators (79.8%) and sealed radioisotopes (15.1%) were surveyed using a questionnaire. Results and Discussion: The perception that regulation is stronger than the actual risk of the radiation source used was 3.47 points (out of 5 points), indicating a score above average. The most important factors and considerations were education and training (48%) as a human factor, safety devices of the radiation source (71.3%) as a hazardous material factor, the use of radiation (50.8%) as an organizational environment, and the radiation effect of nearby facilities (67.2%) as a physical environment. Radiation safety management educational experience (F= 5.030, p< 0.01), the group with high subjective knowledge (t= 6.017, p< 0.001), and the group with high objective knowledge (t= 1.989, p< 0.05) was found to be better at radiation safety management. Conclusion: It is necessary to standardize the educational experience regarding radiation safety management because each staff member has individual differences in educational experience. It is necessary to provide more information on how to solve radiation accidents via educational content. Applying radiation safety regulations based on the factors that significantly affect radiation safety management shown in this survey will help improve safety.

• 가정과삶의질연구
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• 제24권1호
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• pp.31-41
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• 2006

This study was to investigate differences in peer attachment, emotion regulation style and depression perceived by adolescents according to age and gender of adolescents. Also, aim that identifies variables which have influences on adolescents' depression. The subjects were 2279 middle and high school students(male 1150, female 1129) who live in Seoul and Kyonggi. All respondents answered by self-report questionnaire. The scales of emotion regulation style consist of three parts: support-seeking, avoidance/distraction, and active problem-solving. Data were analyzed by two-way ANOVA, t-tset and stepwise multiple regression. The major findings were as follows. First, older male and female adolescents perceived less peer attachment than younger adolescents. Specially female adolescents showed more differences between older adolescents and younger adolescents. Second, male adolescents showed more avoidance/distraction style and active problem-solving style but less support-seeking style than female adolescents. Third, older adolescents perceived more depression than younger adolescents. Fourth, peer attachment, age, avoidance/distraction style, active problem-solving style, support-seeking style and gender had a significant effect on adolescents' depression. In other words, adolescents who had more avoidance/distraction style and support-seeking style, who had less peer attachment and active problem-solving style, who had more avoidance/distraction style and support-seeking style, who had less peer attachment and active problem-solving style, who was older adolescents than younger adolescents, and who was female adolescents than male adolescents perceived more depression. The study results suggest that the degree of peer attachment was the most powerful predictor of adolescents' depression.