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Enhancement of PVA-Degrading Enzyme Production by the Application of pH Control Strategy

  • Li, Min;Zhang, Dongxu;Du, Guocheng;Chen, Jian
    • Journal of Microbiology and Biotechnology
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    • v.22 no.2
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    • pp.220-225
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    • 2012
  • In batch culture for Poly(vinyl alcohol) (PVA)-degrading enzyme (PVAase) production by a mixed culture, higher pH (pH 7.5) was favorable for PVAase production at the prophase of cultivation, but lower pH (pH 7.0) was favorable at the anaphase. This situation was caused by the fact that the optimum pH for different key enzymes [PVA dehydrogenase (PVADH) and oxidized PVA hydrolase (OPH)] production is various. The activity and average specific production rate of PVADH reached the highest values at constant pH 7.5, whereas those of OPH appeared at pH 7.0. A two-stage pH control strategy was therefore developed and compared for its potential in improving PVAase production. By using this strategy, the maximal PVAase activity reached 2.05 U/ml, which increased by 15.2% and 24.2% over the fermentation at constant pH 7.5 and 7.0.

Incremental Passivity Based Control for DC-DC Boost Converters under Time-Varying Disturbances via a Generalized Proportional Integral Observer

  • He, Wei;Li, Shihua;Yang, Jun;Wang, Zuo
    • Journal of Power Electronics
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    • v.18 no.1
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    • pp.147-159
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    • 2018
  • In this paper, the voltage tracking control of a conventional DC-DC boost converter affected by unknown, time-varying circuit parameter perturbations is investigated. Based on the fundamental property of incremental passivity, a passivity based control law is designed. Then, to obtain a better disturbance rejection property, two generalized proportional integral (GPI) observers are employed to estimate the time-varying uncertainties in the output voltage and inductor current channels, and the estimated values are applied as feedforward compensation. Moreover, the global trajectory tracking performance of a system with disturbances is ensured under the composite controller. Finally, simulation and experiment studies are provided to demonstrate the feasibility and effectiveness of the proposed method. The results show that the proposed controller delivers a promising disturbance rejection capability as well as a good nominal tracking performance.

Radiation induced grain boundary segregation in ferritic/martensitic steels

  • Xia, L.D.;Ji, Y.Z.;Liu, W.B.;Chen, H.;Yang, Z.G.;Zhang, C.;Chen, L.Q.
    • Nuclear Engineering and Technology
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    • v.52 no.1
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    • pp.148-154
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    • 2020
  • The radiation induced segregation of Cr at grain boundaries (GBs) in Ferritic/Martensitic steels was modeled assuming vacancy and interstitialcy diffusion mechanisms. In particular, the dependence of segregation on temperature and grain boundary misorientation angle was analyzed. It is found that Cr enriches at grain boundaries at low temperatures primarily through the interstitialcy mechanism while depletes at high temperatures predominantly through the vacancy mechanism. There is a crossover from Cr enrichment to depletion at an intermediate temperature where the Cr:Fe vacancy and interstitialcy diffusion coefficient ratios intersect. The bell-shape Cr enrichment response is attributed to the decreasing void sinks inside the grains as temperature rises. It is also shown that low angle grain boundaries (LAGBs) and special Σ coincidence-site lattice (CSL) grain boundaries exhibit suppressed radiation induced segregation (RIS) response while high angle grain boundaries (HAGBs) have high RIS segregation. This different behavior is attributed to the variations in dislocation density at different grain boundaries.

Factors governing dynamic response of steel-foam ceramic protected RC slabs under blast loads

  • Hou, Xiaomeng;Liu, Kunyu;Cao, Shaojun;Rong, Qin
    • Steel and Composite Structures
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    • v.33 no.3
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    • pp.333-346
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    • 2019
  • Foam ceramic materials contribute to the explosion effect weakening on concrete structures, due to the corresponding excellent energy absorption ability. The blast resistance of concrete members could be improved through steel-foam ceramics as protective cladding layers. An approach for the modeling of dynamic response of steel-foam ceramic protected reinforced concrete (Steel-FC-RC) slabs under blast loading was presented with the LS-DYNA software. The orthogonal analysis (five factors with five levels) under three degrees of blast loads was conducted. The influence rankings and trend laws were further analyzed. The dynamic displacement of the slab bottom was significantly reduced by increasing the thickness of steel plate, foam ceramic and RC slab, while the displacement decreased slightly as the steel yield strength and the compressive strength of concrete increased. However, the optimized efficiency of blast resistance decreases with factors increase to higher level. Moreover, an efficient design method was reported based on the orthogonal analysis.

Arch-to-beam rigidity analysis for V-shaped rigid frame composite arch bridges

  • Gou, Hongye;Pu, Qianhui;Zhou, Yang;Hong, Yu
    • Steel and Composite Structures
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    • v.19 no.2
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    • pp.405-416
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    • 2015
  • We proposed the concept of nominal rigidity of a long-span V-shaped rigid frame composite arch bridge, analyzed the effects of structural parameters on nominal rigidity, and derived a theoretical nominal rigidity equation. In addition, we discussed the selection of the arch-to-beam rigidity ratio and its effect on the distribution of internal forces, and analyzed the influence of the ratio on the internal forces. We determined the delimitation value between rigid arch-flexible beam and flexible arch-rigid beam. We summarized the nominal rigidity and arch to beam rigidity ratios of existing bridges. The results show that (1) rigid arch-flexible beam and flexible arch-rigid beam can be defined by the arch-to-beam rigidity ratio; (2) nominal rigidities have no obvious differences among the continuous rigid frame composite arch bridge, V-shaped rigid frame bridge, and arch bridge, which shows that nominal rigidity can reflect the global stiffness of a structure.

Structural evaluation of a foldable cable-strut structure for kinematic roofs

  • Cai, Jianguo;Zhang, Qian;Zhang, Yiqun;Lee, Daniel Sang-hoon;Feng, Jian
    • Steel and Composite Structures
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    • v.29 no.5
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    • pp.669-680
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    • 2018
  • The rapidly decreasing natural resources and the global variation of the climate push us to find intelligent and efficient structural systems to provide more people with fewer resources. This paper proposed a kinematic cable-strut system to realize sustainable structures in responding to changing environmental conditions. At first, the concept of the kinematic system based on crystal-cell pyramid (CP) cable-strut unit was given. Then the deployment of the structure was studied experimentally. After that, the static behaviors in the fully deployed state under the symmetric and asymmetric load cases were investigated. Moreover, the effects of thermal loading and the initial prestress distribution were also discussed. Comparative studies between the proposed structure and other deployable cable-strut system under three times of design load cases were carried out. Finally, the robustness of the system was studied by removal of one passive cable at one time.

Interface monitoring of steel-concrete-steel sandwich structures using piezoelectric transducers

  • Yan, Jiachuan;Zhou, Wensong;Zhang, Xin;Lin, Youzhu
    • Nuclear Engineering and Technology
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    • v.51 no.4
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    • pp.1132-1141
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    • 2019
  • Steel-concrete-steel (SCS) sandwich structures have important advantages over conventional concrete structures, however, bond-slip between the steel plate and concrete may lead to a loss of composite action, resulting in a reduction of stiffness and fatigue life of SCS sandwich structures. Due to the inaccessibility and invisibility of the interface, the interfacial performance monitoring and debonding detection using traditional measurement methods, such as relative displacement between the steel plate and core concrete, have proved challenging. In this work, two methods using piezoelectric transducers are proposed to detect the bond-slip between steel plate and core concrete during the test of the beam. The first one is acoustic emission (AE) method, which can detect the dynamic process of bond-slip. AE signals can be detected when initial micro cracks form and indicate the damage severity, types and locations. The second is electromechanical impedance (EMI) method, which can be used to evaluate the damage due to bond-slip through comparing with the reference data in static state, even if the bond-slip is invisible and suspends. In this work, the experiment is implemented to demonstrate the bond-slip monitoring using above methods. Experimental results and further analysis show the validity and unique advantage of the proposed methods.

Dynamic response of railway vehicles under unsteady aerodynamic forces caused by local landforms

  • Chen, Zhengwei;Liu, Tanghong;Li, Ming;Yu, Miao;Lu, Zhaijun;Liu, Dongrun
    • Wind and Structures
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    • v.29 no.3
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    • pp.149-161
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    • 2019
  • When a railway vehicle runs in crosswinds, the unsteady aerodynamic forces acting on the train induced by the vehicle speed, crosswind velocity and local landforms are a common problem. To investigate the dynamic performance of a railway vehicle due to the influence of unsteady aerodynamic forces caused by local landforms, a vehicle aerodynamic model and vehicle dynamic model were established. Then, a wind-loaded vehicle system model was presented and validated. Based on the wind-loaded vehicle system model, the dynamic response performance of the vehicle, including safety indexes and vibration characteristics, was examined in detail. Finally, the effects of the crosswind velocity and vehicle speed on the dynamic response performance of the vehicle system were analyzed and compared.

Fabrication of triazine-based Porous Aromatic Framework (PAF) membrane with structural flexibility for gas mixtures separation

  • Wang, Lei;Jia, Jiangtao;Faheem, Muhammad;Tian, Yuyang;Zhu, Guangshan
    • Journal of Industrial and Engineering Chemistry
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    • v.67
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    • pp.373-379
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    • 2018
  • A transparent, freestanding Porous Aromatic Framework-97 (PAF-97) membrane was successfully synthesized via a one-step acid-catalyzed reaction. Due to the introduction of ether groups, the obtained PAF-97 membrane possesses enhanced structural flexibility, thus increasing the flexibility of the resulting membrane. This is proofed by the fact that the feeding pressure of the membrane reaches as high as 5.5 bar during the separation of gas mixtures. The Young's moduli of the membrane were 6.615 GPa and 11.11 GPa, either in a dry or hydrated state respectively. To be highlighted, under a feeding pressure of 3.6 bar, the PAF-97 membrane rendered the permeance values of $2.90{\times}10^{-7}$, $1.29{\times}10^{-8}mol\;m^{-2}s^{-1}Pa^{-1}$ for $CO_2$ and $CH_4$, respectively, with a $CO_2/CH_4$ permselectivity of 22.48.

Accurate Detection of a Defective Area by Adopting a Divide and Conquer Strategy in Infrared Thermal Imaging Measurement

  • Jiangfei, Wang;Lihua, Yuan;Zhengguang, Zhu;Mingyuan, Yuan
    • Journal of the Korean Physical Society
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    • v.73 no.11
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    • pp.1644-1649
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    • 2018
  • Aiming at infrared thermal images with different buried depth defects, we study a variety of image segmentation algorithms based on the threshold to develop global search ability and the ability to find the defect area accurately. Firstly, the iterative thresholding method, the maximum entropy method, the minimum error method, the Ostu method and the minimum skewness method are applied to image segmentation of the same infrared thermal image. The study shows that the maximum entropy method and the minimum error method have strong global search capability and can simultaneously extract defects at different depths. However none of these five methods can accurately calculate the defect area at different depths. In order to solve this problem, we put forward a strategy of "divide and conquer". The infrared thermal image is divided into several local thermal maps, with each map containing only one defect, and the defect area is calculated after local image processing of the different buried defects one by one. The results show that, under the "divide and conquer" strategy, the iterative threshold method and the Ostu method have the advantage of high precision and can accurately extract the area of different defects at different depths, with an error of less than 5%.