• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.107-114
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• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• Journal of Internet Computing and Services
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• v.4 no.2
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• pp.31-37
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• 2003

Recently, motion capture has become one of the most promising technologies in animation. Realistic motion data can be captured by recording the movement of a real actor with an optical or magnetic motion capture system. This paper deals with motion editing by a geometric mean filter. Since the captured motion has some noises that cause a jerky motion, it needs a smoothing process to make it natural. A geometric mean filter is proposed to produce natural motions without jerky motions. Experimental results show that the geometric mean filter can effectively remove noises that cause a jerky motion and it can guarantee the most natural motions among various spatial filters. This method could be applied to the various fields such as real time animation, virtual reality applications, 3D applications, and etc.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.154-160
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• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.

• Journal of Internet Computing and Services
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• v.5 no.2
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• pp.41-47
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• 2004

Recently, motion capture has become one of the most promising technologies in animation. Realistic motion data can be captured by recording the movement of a real actor with an optical or magnetic motion capture system. This paper deals with motion editing by a geometric mean filter. Since the captured motion has some noises that cause a jerky motion, it needs a smoothing process to make it natural. A geometric mean filter is proposed to produce natural motions without jerky motions. Experimental results show that the geometric mean filter can effectively remove noises that cause a jerky motion and it can guarantee the most natural motions among various spatial filters. This method could be applied to the various fields such as real time animation, virtual reality applications, 3D applications, and etc.

• Journal of Biomedical Engineering Research
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• v.22 no.1
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• pp.81-90
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• 2001

본 연구에서는 디지털 영상 뇌파계(digital video electroencephalogram, Digital VEEG)에서 비디오 영상과 뇌전도 파형의 동기화된 편집 시스템을 구성한다. 이 시스템은 기존 아날로그 영상 뇌파계(analog video electroencephalogram)의 동기화 문제와 디지털 영상 시스템에서의 영상편집 문제를 해결하기 위하여 MPEG-I(이하 MPEG) 고압축 기술을 이용한 MPEG 인코딩 보드(encoding board)와 MPEG 편집 엔진(editing engine)을 각각 사용하였다. 시스템은 디지털 영상뇌파계모듈과 디지털 편집 모듈로 구성되며, 뇌전도모듈에서는 환자에게 연결된 전극을 통해 들어온 뇌파를 생체신호증폭기를 이용하여 증폭한 후 AD 보드(analog to digital board)를 이용 디지털화한다. 디지털 카메라로 촬영된 환자영상의 아날로그 영상신호(NTSC 신호)는 MPEG 인코딩 보드를 이용하여 고압축 디지털화한다. 이후 디지털화된 뇌전도신호와 MPEG 형식의 영상을 시간 동기화하여 두 개의 모니터에 각각보여준다. 편집 모듈에서는 영상신호와 뇌파신호를 어느 부분이든 간단한 조작으로 오려 붙이기(cut and paste) 기능을 이용할 수 있다. 본 시스템은 사용된 데이터 모두 디지털 기술을 이용하여 영상과 뇌파신호의 정확한 동기화 및 각각의 데이터의 오려 붙이기 기능을 가능케 하였으며, 이는 환자의 데이터를 관리 및 보관하는데 있어, 임상의에게 의미 있는 자료만을 모아서 효율적으로 관리할 수 있게 해준다. 이와 같은 장점을 갖는 디지 영상뇌파계 편집시스템을 구현하였다.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• The KIPS Transactions:PartD
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• v.9D no.3
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• pp.487-494
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• 2002

In this paper, we present an equation edition system, which is designed and implemented according to the W3C MathML standard. The system can be used to edit equations for the various web-oriented applications. Various user-friendly interfaces are provided for the easy and efficient edition of equations. The edited equations can be viewed immediately with the representation modules. To improve the processing speed of equations, we upgraded functions of the conventional XML parser. The presented system will contribute to the extensive usage of equations on the web and efficient processing of XML documents with equations.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.3
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• pp.129-141
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• 2005

It is difficult to edit large-scale digital maps because of problems of cost and process and it is carried out by aerial photogrammetry in renewal periods. Five years of update period cannot provide exact data required in the fast-moving age. This study is to analyze applicability and impact for editing digital map of road system using the GPS-Van. Results are compared with accuracy of the data acquisition with GPS-Van positioning. INS data are affected by the barrier to receive GPS data. But high accuracy were achieved by thorough plans according to work order. By using GPS Van and fieldwork at the same time for editing and renewal of digital map, it is expected that this method can be used to reduce costs in the economic and temporal aspects, and provide rapid and accurate digital map of road system.

• Biomedical Science Letters
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• v.26 no.2
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• pp.114-119
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• 2020

Of the various types of mice used for genome editing, conditional knock-out (cKO) mice serve as an important model for studying the function of genes. cKO mice can be produced using loxP knock-in (KI) mice in which loxP sequences (34 bp) are inserted on both sides of a specific region in the target gene. These mice can be used as KO mice that do not express a gene at a desired time or under a desired condition by cross-breeding with various Cre Tg mice. Genome editing has been recently made easy by the use of third-generation gene scissors, the CRISPR-Cas9 system. However, very few laboratories can produce mice for genome editing. Here we present a more efficient method for producing loxP KI mice. This method involves the use of an HDR vector as the target vector and ssODN as the donor DNA in order to induce homologous recombination for producing loxP KI mice. On injecting 20 ng/µL of ssODN, it was observed that the target exon was deleted or loxP was inserted on only one side. However, on injecting 10 ng/µL of the target HDR vector, the insertion of loxP was observed on both sides of the target region. In the first PCR, seven mice were identified to be loxP KI mice. The accuracy of their gene sequences was confirmed through Sanger sequencing. It is expected that the loxP KI mice produced in this study will serve as an important tool for identifying the function of the target gene.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.4
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• pp.798-803
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• 2004

XML(extensible Markup Language) of W3C(World Wide Web Consortium) that is used the standard core technology of data exchange on the current Internet is an independent data type of usable at the all platforms. Especially, it can handle rapidly because of the integration of each other data types that is exchanged. Between each application and system that built at an enterprise in the past. However, W3C had notice to use XSLT(extensible Stylesheet Language Transformation) that is document transformable standard to descript expression information in XML documents because documents of XML only have a logical structure information. It is designed for XML that is developed for data exchange on the internet. Moreover, it is proposed to process and to change as other data type for expression XML documents for user. This thesis design and implement XSLT document editing system transformable as a un data type as a HTML data type applying XSLT at XML and developed the system. It can edit XSLT document that descript expression information in XML document that is used for data editing in the WYSIWYG environment.