• Journal of Animal Reproduction and Biotechnology
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• v.35 no.4
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• pp.323-328
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• 2020

Direct injection of genome editing tools such as CRISPR/Cas9 system into developing embryos has been widely used to generate genetically engineered pigs. The approach allows us to produce pigs carrying targeted modifications at high efficiency without having to apply somatic cell nuclear transfer. However, the targeted modifications during embryogenesis often result in mosaicism, which causes issues in phenotyping founder animals and establishing a group of pigs carrying intended modifications. This study was aimed to establish a genomic PCR and sequencing system of a single blastomere in the four-cell embryos to detect potential mosaicism. We performed genomic PCR in four individual blastomeres from four-cell embryos. We successfully amplified target genomic region from single blastomeres of 4-cell stage embryo by PCR. Sanger sequencing of the PCR amplicons obtained from the blastomeres suggested that PCR-based genotyping of single blastomere was a feasible method to determine mutation type generated by genome editing technology such as CRISPR/Cas9 in early stage embryos. In conclusion, we successfully genotyped single blastomeres in a single 4-cell stage embryo to detect potential mosaicism in porcine embryos. Our approach offers a simple platform that can be used to screen the prevalence of mosaicism from designed CRISPR/Cas9 systems.

• Tunnel and Underground Space
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• v.11 no.1
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• pp.64-71
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• 2001

The purpose of this study is to develop rail rockfall and landslide information system to manage spatial database using GIS. For this, a spatial database containing information such as railroad, map, topographic analysis, meteorological data, and rockfall has been constructed for 2.5 km or 5 km buffer zone from the Kyungchun, Youngdong, Jungang, Taebak and Jungsun Rairoad where risk of landslide occurrence potential is high. For management of the spatial database, railroad rockfall and landslide information system has been developed. The information system consists of view, table, chart, layout and project environment. The functions provided in the system are data conversion, editing, labeling, zoom in and out, map making, graphic editing, text DB management, charting, on-line help as well as input, retrieve and output of spatial database. The system was developed using ArcView script language Avenue, and consisted of pull-down menus and icons for easy use. The spatial database and the information system can be used to rockfall and landslide management and analysis near the railroad as basic data and tool.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.4
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• pp.857-862
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• 2013

Due to the development of the online and mobile, cases of introducing CMS(Contents Management System) and WCMS(Web Contents Management System) to manage Web-Contents of individual and company's homepage are continuously increasing. In this paper, it is necessary to develop WCMS that can establish GUI(Graphic User Interface) based WCMS and edit contents using more concise procedures. In this paper, by reconfiguring WCMS-based Web contents using user-definition, we designed the system that can effectively and conveniently edit web contents as GUI-based. Also, we studied the intuitive and convenient web-contents management methods through researches using simplified logic. Therefore, proposed WCMS based Web-contents editing system allows the users to edit and modify the web contents much more quickly and conveniently compared to the existing web-contents editing system.

• Journal of the Korean Institute of Intelligent Systems
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• v.17 no.4
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• pp.494-499
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• 2007

In this paper we realized a system, if a user selects the area of the important parts or the arrangement parts when he reads the books or the papers, which amends, stores and readjusts the characters that are included in the selected area by outputting the characters to the word processor in sequence. If a user selects what he wishes lot with his finger, the system detects the movement of the finger by applying the hand recognition algorithm and recognizes the selected area. The system converts the distance of the width and the length of the selected area to the number of the pulse, and controls the motor to move the camera at the position. After the system scales up/down the zoom to be able to recognize the character and controls the focus to the regulated zoom closely, it controls the focus in detail to get more distinct image by using the difference of the light and darkness. We realize the recognition and editing support system of korean language that converts the obtained images to the document by applying the character recognition algorithm and arrange the important parts.

• Proceedings of the IEEK Conference
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• 2002.06c
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• pp.223-226
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• 2002

If one can easily modify the existing motion data to a new motion in making an animation movie, he can save a lot of time for graphic design. To implement this kind of system, we propose a PC-based system composed of low cost commercial animation tool (3D Studio Max) for visualization of the animation and motion editing module that handles optimization process during the motion transform. Researchers studying advanced motion transform techniques only have to focus on the mathematical manipulation of the motion data

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 1996.06b
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• pp.77-81
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• 1996

In order to conform to the needs of effective program production in multimedia era, we are studying Desk Top Program Production system. With the DTPP, users can easily produce multimedia program including video, sound, and ancillary data, and freely handle video images synthesizing video components retrieved from video database. This paper describes the new program production system, DTPP and its key technologies such as cooperative program production via multimedia network, indexing and utilization of attribute information of images, and image segmentation and spatio-temporal editing.

• International Journal of Industrial Entomology and Biomaterials
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• v.39 no.1
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• pp.14-21
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• 2019

Genome editing by CRISPR/Cas9, a third-generation gene scissor in molecular breeding at the genome level, is attracting much attention as one of the breeding techniques of the future. In this study, genetic and phenotypic analysis was used to examine the responsiveness of the Bakokjam variety of the silkworm Bombyx mori to molecular breeding using CRISPR/Cas9 in editing the white egg 2 (w-2) gene. The nucleotide sequence of the w-2 gene was analyzed and three different guide RNAs (gRNA) were prepared. The synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the Bm-N silkworm cell line. To edit the silkworm gene, W1N and W2P gRNA and Cas9 complexes were microinjected into silkworm embryos. Based on the results of microinjection, the hatching rate was 16-24% and the incidence of mutation was 33-37%. The gene mutation was verified in the heterozygous F1 generation, but no phenotypic change was observed. In F2 homozygotes generated by F1 self-crosses, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and will be a very effective way to shorten the time required than the traditional breeding process.

• Journal of Digital Convergence
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• v.19 no.7
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• pp.199-208
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• 2021

Automatic Post Editing(APE) is the study that automatically correcting errors included in the machine translated sentences. The goal of APE task is to generate error correcting models that improve translation quality, regardless of the translation system. For training these models, source sentence, machine translation, and post edit, which is manually edited by human translator, are utilized. Especially in the recent APE research, multilingual pretrained language models are being adopted, prior to the training by APE data. This study deals with multilingual pretrained language models adopted to the latest APE researches, and the specific application method for each APE study. Furthermore, based on the current research trend, we propose future research directions utilizing translation model or mBART model.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.05a
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• pp.531-534
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• 2008

Recently, by the interest of geographical information and the utilization field of geographical information diversifying. Various GIS(Geographic Information System) was built to manage geographical information efficiently. However, because of interoperability between geographic information system, OGC(Open Geospatial Consortium) standardized GML(Geography Markup Language) of the XML(eXtensible Markup Language) foundation in which an interoperability writes possible geographical information data. GML national standard is applied to various application fields at present, and the use is confirmed. In this paper, GML 3.2.1 encoding standard specification follows requirements of OGC recommendation, we designed GML document editing system using XML standard technology, and generated interoperability GML data.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.6
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• pp.1058-1064
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• 2009

According to development of Information Technology and generalization of internet, practical use field of geography information became various. Therefor various Geographic Information System (GIS) constructed to manage geography information efficiently. However, geography information data of various form is depending on graphic authorizing tool of various form being not normalized each other. So, OGC(Open Geospatial Consortium) proposed GML(Geography Markup Language) that describe normalized geography information data that can apply mutually and W3C proposed SVG(Scalable Vector Graphics) of vector base. In this paper creates GML data of XML base for geography information data processing to vector graphic object, and design and implementation of GML document editing and translation system that define code converter that create GML document through created graphic objects and change vector graphic to logic structure of XML base.