• 한국동물위생학회지
• /
• 제41권4호
• /
• pp.257-262
• /
• 2018

Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

• 디지털융복합연구
• /
• 제16권11호
• /
• pp.89-96
• /
• 2018

현대사회에서는 경제성장이 중시되던 과거에 비해 삶의 질 향상과 행복을 위한 노력이 중시되고 있다. 이에 정부는 문화를 통한 국민행복실현을 이루고자 다양한 정책을 펼치고 있다. 본 연구는 기존의 한정된 설문조사나 문헌조사와는 달리 문화예술을 독립변수로 설정하여 수요(향유)와 공급 측면으로 나눈 후, 종속변수로 서울시민의 행복지수 향상에 미치는 영향을 소득과 인구구조변화의 통제 하에 시간적 범위를 10년 이상 설정한 후 정량적으로 패널분석 하였다. 분석결과 문화예술에 대한 향유가 클수록 서울시민의 행복지수 향상에 양(+)의 영향을 끼쳤으나 문화예술 공급은 유의미하지 못한 영향을 미치는 것으로 밝혀졌다. 이는 문화예술 향유에 기인한 서울시민의 행복지수 향상이 이루어지고 있으며 공급을 통한 서울시민의 행복지수를 향상시키기 위해서는 기존의 공간을 보완하고 다양한 계층을 겨냥한 차별화된 문화예술프로그램을 통해 문화수요계층 뿐만 아니라 문화무관심집단 나아가서는 문화소외계층의 능동적 참여를 증가시켜야 한다는 정책적 시사점을 줄 수 있다.

• 한국치위생학회지
• /
• 제19권2호
• /
• pp.287-295
• /
• 2019

Objectives: The purpose of this study was to investigate the factors affecting subjective oral symptoms according to the gender of youth from multi-cultural families in Korea using data from the 14th (2018) Korean Youth Health Behavior Survey. Methods: The independent variables used in this study consisted of gender and sweet drink intake. The dependent variable was experience of subjective oral symptoms. Compensation variables consisted of general characteristics of school type, academic performance, economic status, drinking status, smoking status, and number of tooth brushings day before. The subjects of the study were 835 children of multi-cultural families whose parents were foreigners. All statistical analyses were performed by complex samples cross-tabulation analysis and complex samples logistic regression analysis. Statistical analysis was performed using the PASW statistical package 21.0 (Statistical Packages for Social Science Inc., Chicago, IL, USA). A significance level of 0.05 was used for statistical significance. Results: The composite sample logistic regression analysis showed that there was a statistically significant difference between gender and intake of sweet drinks in experience of subjective oral symptoms. Conclusions: These results suggest that factors influence subjective oral symptoms in Korean multi-cultural adolescents. Therefore, I hope that they will be used as basic data for the introduction and development of a customized oral health education program for improving oral health of multi-cultural adolescents.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권6호
• /
• pp.891-895
• /
• 2019

Objective: Aim of present study was the set up of a fast and reliable protocol using species-specific markers for the quali-quantitative analysis of DNA and the detection of ruminant biological components in dairy products. For this purpose, the promoter of the gene coding for the ${\alpha}$-lactoalbumin (LALBA) was chosen as possible candidate for the presence of short interspersed nuclear elements (SINEs). Methods: DNA was isolated from somatic cells of 120 individual milk samples of cattle (30), Mediterranean river buffalo (30), goat (30), and sheep (30) and the gene promoter region (about 600/700 bp) of LALBA (from about 600 bp upstream of exon 1) has been sequenced. For the development of a single polymerase chain reaction (PCR) protocol that allows the simultaneous identification of DNA from the four species of ruminants, the following internal primers pair were used: 5'-CACTGATCTTAAAGCTCAGGTT-3' (forward) and 5'-TCAGA GTAGGCCACAGAAG-3' (reverse). Results: Sequencing results of LALBA gene promoter region confirmed the presence of SINEs as monomorphic "within" and variable in size "among" the selected species. Amplicon lengths were 582 bp in cattle, 592 bp in buffalo, 655 in goat and 729 bp in sheep. PCR specificity was demonstrated by the detection of trace amounts of species-specific DNA from mixed sources ($0.25ng/{\mu}L$). Conclusion: We developed a rapid PCR protocol for the quali-quantitative analysis of DNA and the traceability of dairy products using a species-specific marker with only one pair of primers. Our results validate the proposed technique as a suitable tool for a simple and inexpensive (economic) detection of animal origin components in foodstuffs.

• 응용통계연구
• /
• 제34권4호
• /
• pp.537-556
• /
• 2021

통계적 공정관리절차의 특성은 해석적 해를 얻기가 어려운 경우가 많이 있으나 Markov연쇄를 적용하면 가능한 경우가 많이 있다. 이 논문에서는 공정 통계량이 Markov특성을 따르는 경우, Markov연쇄를 생성하는 방법과 이를 이용한 공정관리 절차의 특성을 도출하는 방법에 대해 설명하고 있다. 관리도의 통계적 설계, 경제적 설계 및 변량표본 추출비 설계 등의 특성 규명을 위한 Markov연쇄의 적용에 대한 기존의 알려진 방법을 복습하고 또한 새로운 공정관리 분야인 재조정 관리도에의 적용방법에 대한 연구결과도 보여주고 있다. 공정관리의 특성연구에서 해석적 해가 가능한 경우에도 이 과정이 복잡하여 Markov연쇄를 병행 사용하면 특성 규명이 명확해지며, 모의실험보다는 짧은 시간에 더 정밀한 결과를 얻을 수 있어 널리 이용되고 있다.

• 보건의료산업학회지
• /
• 제13권3호
• /
• pp.149-162
• /
• 2019

Objectives: The purpose of this study was to identify differences in characteristics of the people who frequently drink alone between those in old-city regions and those in other regions in Busan, focusing on socio-demographic factors and the level of drinking according to the types of stress. Methods: The analysis confirmed the differences based on Busan Social Statistics Data in 2012, 2014, and 2016. Frequencies by each variable were presented, and ${\chi}^2$ and logistic regression analysis were also used in the analysis. Results: The residents of Busan showed higher odds ratios of "frequent drinking alone" when they felt considerably higher-than-normal levels of stress. In addition, in the residents of old-city regions, the odds ratios of those who frequently drank alone were not different between people who could easily relieve stress, who experienced economic stress, or who had relationship stress and people who did not. However, higher odds ratios of frequently drinking alone were reported when such participants felt some stress related to work, family, relationships, and so on. Conclusions: Interventions for the appropriate relief of stress caused by different reasons, as well as other stress-relieving methods, should be implemented for the people's benefits.

• 한국융합학회논문지
• /
• 제12권6호
• /
• pp.139-150
• /
• 2021

현재 국내 신재생에너지 보급 정책은 RPS(Renewable Portfolio Standard; 공급의무화제도)이다. 대용량 발전사업자는 신재생 의무공급량을 직접 생산하거나 REC(Renewable Energy Certificates; 공급인증서) 구매를 통해서 확보한다. 정부는 신재생 에너지원의 경제적, 사회적 가치를 평가하여 각 에너지원에 가중치를 매기는 REC 가중치를 여러 차례 개정했으며 의무공급 비율도 점차 증가하도록 개정했다. 본 연구는 정부의 정책이 관련 산업에 미치는 영향을 확인하는 데 도움이 된다. 태양광발전에 관한 REC 가중치 개정과 신재생 의무공급량이 태양광 설비량에 미치는 영향을 분석하기 위해 태양광 설비량을 종속변수로 시계열 분석과 의무공급량에 대한 회귀분석을 수행하였다. 결과적으로 REC 가중치 개정과 의무공급량 증가는 태양광 설비량 증가에 유의미한 영향을 미친다는 것을 확인하였다.

• 한국가족관계학회지
• /
• 제23권2호
• /
• pp.133-155
• /
• 2018

Objectives: This study examined the differences in the intention of having a second child and the related factors among Chinese and Vietnamese migrant women from the perspective of adaptation theory. Methods: Data were drawn from the National Survey on Multi-Cultural Families in 2009 and 2015. Among the total 7,615 married migrant women (Korean-Chinese, Chinese-Han, Vietnamese), those within the age group 20-39 within the first 5 years of marriage who had one child were selected. A frequency analysis, chi-squared test, and logit regression analysis were performed. Results: Different ethnic groups had different reasons for having a second child and the related factors also differed between 2009 and 2015. In 2009, after controlling the related variables, the intention of Korean-Chinese and Chinese-Han married immigrant women to have a second child was higher than that of Vietnamese women, but no such difference was found in 2015. Participation in their local community, first marriage, the gender of the first child, and whether they were living with their parents-in-law were associated with the intention of migrant women having a second child in the 2009 analysis model but these factors were not significant in the 2015 analysis model. In the latter model, the household income, a variable related to economic conditions, has a positive effect on the intention of having a second child. Conclusions: The significance of this study supports adaptation theory by addressing the similarity in the childbirth intention between recently married immigrant women and Korean women.

• 한국압력기기공학회 논문집
• /
• 제16권2호
• /
• pp.1-9
• /
• 2020

Large-scale industrial facility structures continue to deteriorate due to the effects of operating and environmental conditions. The problems of these industrial facilities are potentially causing economic losses, environmental pollution, casualties, and national losses. Accordingly, in order to prevent disaster accidents of large structures in advance, the necessity of diagnosing structures using non-destructive inspection techniques is being highlighted. The defect occurrence, location and defect type of the structure are important parameters for predicting the remaining life of the structure, so continuous defect observation is very important. Recently, many researchers have been actively researching real-time monitoring technology to solve these problems. Structure Health Monitoring Inspection is a technology that can identify and respond to the occurrence of defects in real time, but there is a limit to check the degree of defects and the direction of growth of defects. In order to compensate for the shortcomings of these technologies, the importance of defect imaging techniques is emerging, and in order to find defects in large structures, a method of inspecting a wide range using guided ultrasonic is effective. The work presented here introduces a calculation for the shape factor for evaluation of the damaged area, as well as a variable β parameter technique to correct a damaged shape. Also, we perform research in modeling simulation and an experiment for comparison with a suggested inspection method and verify its validity. The curved structure image obtained by the advanced RAPID algorithm showed a good match between the defect area and the shape.

• 융합경영연구
• /
• 제8권2호
• /
• pp.13-17
• /
• 2020

Purpose: In modern corporate management, the establishment of a crisis management system that minimizes damage through measures used to respond to corporate crises is no longer an option. The importance of corporate reputation and brand asset management in modern enterprise management cannot be overemphasized and negative events that might arise from a number of different causes can cause brand crises. Research design, data and methodology: More than half of the questionnaire respondents were female (252 or 53%). More than a fourth of the respondents were aged 20 (122 or 26%) and the number of married participants was 196 (41%). Of the participants, 32% (153) had graduated from college. Only 18% (87) were employees and the monthly household income was 121. In this study, we conducted factor analysis in order to extract the variables that may enhance the explanation capability of each variable. For the method of factor extraction, an Eigen value of at least 1 was used as was factor loading. An analysis was performed using the Cronbach's alpha coefficient to verify the reliability of the measurement scale. Results: First, the analysis of the impact of the social responsibility activities on brand image revealed that the social, economic, philanthropic, ethical, and environmental responsibility activities significantly affected brand image, but legal responsibility activities were not statistically significant. Second, the analysis of the impact of brand image on loyalty showed that brand image had a significant impact on loyalty. Third, the analysis of the impact of social responsibility activities on loyalty showed that they had a significant impact on loyalty. Conclusions: The pro-social enterprise image is not only a brand asset that can be shared, but also a heavy proposition followed by a corresponding social responsibility, it will have to practice transparent corporate management based on clear principles through the establishment of various systems and the implementation of a strict code of conduct within the enterprise.