• Entomological Research
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• v.48 no.6
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• pp.533-539
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• 2018

The RNA interference (RNAi) has been considered as an important genetic tool and applied to develop a new living modified (LM) crop trait which is an improvement of nutrient quality or pest management. The RNAi of DvSnf7 has been used for resistance to LM maize and the Western Corn Rootworm which is a major agricultural pest for the US Corn Belt. Most of the environmental risk assessments (ERA) of double strand RNA (dsRNA) have been performed using in vitro transcript products, and not in vivo expressed product. A large amount of dsRNA was required for the acute toxicity assay of water fleas. Therefore development of massive dsRNA purification techniques is critical. Daphnia, a freshwater microcrustacean, is a model organism for studying cellular and molecular mechanism involved in life history traits and ecotoxicology. In this study, we established the massive dsRNA purification method using Escherichia coli and implemented acute toxicity assays to Daphnia magna. As a result, the present RNase A and DNase I, dsRNA was efficiently purified without any special techniques or equipment. Even though purified dsRNA existed during the acute toxicity test, lethality or abnormal behavior were not observed in D. magna. These results indicated that GFP and DvSnf7 dsRNA were not significantly affected to D. magna due to their lack of sequence matching in its genome. The purification method of dsRNA and the acute toxicity assay of water fleas using purified dsRNA would be suitable for the toxicological studies of LMOs to aquatic non-target organisms.

• Land and Housing Review
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• v.15 no.1
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• pp.77-97
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• 2024

This study aims to examine the distinctions in evaluation index items between overseas and domestic ecological area-related systems, derive analytical indicators, and assess recently completed apartment complexes before and after the implementation of overall ecological area ratios. The objective is to analyse variances in the application of ecological area characteristics, categorizing them into ecological area analysis indicators and presenting their implications. The spatial scope covers completed apartment complexes in both metropolitan and non-metropolitan areas. Thirty-six completed apartment complexes were selected for analysis, and basic ecological area data were compiled. Subsequently, the data was utilized to categorize three analysis indicators-ecological area ratio, ecological area diversity, and ecological area connectivity-by metropolitan and non-metropolitan areas, as well as by type of apartment complex (sale housing versus rental housing) and size (large-scale, medium-scale, and small-scale). Results of the analysis indicate higher ecological area ratios and greater diversity in ecological area spatial types in metropolitan areas compared to non-metropolitan areas, and in pre-sale housing complexes compared to rental housing complexes. Mediumand large-scale apartment complexes exhibit higher ecological area ratios, with ecological area diversity being more pronounced. Ecological area connectivity reveals more numerous and varied connection points and types in metropolitan areas than in non-metropolitan areas. Implications of this study suggest that large-scale development should prioritize securing ecological area ratios and diversity in apartment complexes. Enhancing biodiversity necessitates establishing connections within and beyond the ecological area network of the complex. Future research should focus on linking the ecological area network within the complex.

• Journal of Korean Society of Rural Planning
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• v.24 no.3
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• pp.63-71
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• 2018

The comparison of demand and supply is needed for efficient ecosystem services planning. However, the gap between them cannot be analyzed as existing studies mainly dealt with only the supply of ecosystem services. This study compares the demand and supply of ecosystem services in Shiheung using environmental complaints and urban planning by semantic network analysis. As a result, 'air' and 'water' quality are magnified in demand, 'energy' and 'water' are crucial in supply. This result presents that citizen ask for the improvement of air quality in regulation services, although local government has plans for energy support in provisioning services. Periodic ecosystem services demand and supply monitoring will be the base of effective ecosystem services planning, which reduce insufficiency and surplus.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.1 no.1
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• pp.68-73
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• 2020

Korean fir (Abies koreana) is an evergreen coniferous tree species that is unique to South Korea. A. koreana is found in a limited sub-alpine habitat and is considered particularly vulnerable to climate change. Identification of populations vulnerable to climate change is an important component of conservation programs. In this study, a heat stress-induced transcriptome RNA-seq dataset was used to identify a subset of six genes for assessment as candidate marker genes for ecologically vulnerable populations. Samples of A. koreana were isolated from ecologically stable and vulnerable regions of the Halla and Jiri mountains, and the expression levels of the six candidate markers were assessed using quantitative real-time polymerase chain reaction. All six of the candidate genes exhibited higher expression levels in samples from vulnerable regions compared with stable regions. These results confirm that the six high temperature-induced genes can be used as diagnostic markers for the identification of populations of A. koreana that are experiencing stress due to the effects of climate change.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.515-520
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• 2017

Sexual dimorphism of the mouthpart, antenna and mandible of the Cyllorhynchites ursulus in South Korea was studied with linear measurements. The mouthpart and antenna measurements were conducted with a stereoscopic microscope using 122 specimens (72 males and 50 females). Microscopic observation of the mandible were conducted with a Field Emission Scanning Electron Microscope (FE-SEM) using 103 specimens (73 males and 30 females). Results showed that the size difference between males and females was significant in the size of the mouthpart and antenna. On the other hand, we could not detect sexual size dimorphism in the microstructure of the mandible. The bivariate plots made by the result of Principal Component Analysis (PCA) and Discriminant Analysis (DA) showed a size dimorphism in the size of the mouthpart and the antenna between males and females. Based on our study, sexual dimorphism in the mouthpart and antenna exists in C. ursulus from the South Korean population, and this difference seems to be related to the behavioral differences between males and females.

• Journal of Korean Elementary Science Education
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• v.38 no.4
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• pp.465-474
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• 2019

The purpose of this study was to investigate the effect of the experience-based ecology-environmental STEAM education on ecological sensitivity of elementary students. The research subjects were 62 third graders of S elementary school located in Incheon. They were divided into the experimental group of 33 students and the comparative group of 29 students. The experimental group was provided the educational program with the experience-based ecology-environmental STEAM education. The comparative group was provided the theoretical and ecological self activity program based on the textbook. The results of this study can be summarized as follows; First, the experience-based ecology-environmental STEAM education had a meaningful effect on improvement of ecological sensitivity. Second, we found that access to the ecological environment education is important in all subjects, not in education that is biased toward. Third, in ecological environment education, experience-oriented education methods are more meaningful than indirect experiences trapped in textbooks. Fourth, ecological environment education can contribute to the improvement of ecological sensitivity as well as the intellectual aspect of ecology and environmental science. In conclusion, it was found that the ecological environment program should be conducted with activities of experience.

• Food Science of Animal Resources
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• v.44 no.1
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• pp.165-177
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• 2024

Volatile compounds (VOCs) are an important factor affecting meat quality. However, the characteristic VOCs in different parts of donkey meat remain unknown. Accordingly, this study represents a preliminary investigation of VOCs to differentiate between different cuts of donkey meat by using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with chemometrics analysis. The results showed that the 31 VOCs identified in donkey meat, ketones, alcohols, aldehydes, and esters were the predominant categories. A total of 10 VOCs with relative odor activity values ≥1 were found to be characteristic of donkey meat, including pentanone, hexanal, nonanal, octanal, and 3-methylbutanal. The VOC profiles in different parts of donkey meat were well differentiated using three- and two-dimensional fingerprint maps. Nine differential VOCs that represent potential markers to discriminate different parts of donkey meat were identified by chemometrics analysis. These include 2-butanone, 2-pentanone, and 2-heptanone. Thus, the VOC profiles in donkey meat and specific VOCs in different parts of donkey meat were revealed by HS-GC-IMS combined with chemometrics, whcih provided a basis and method of investigating the characteristic VOCs and quality control of donkey meat.

• The Journal of Sustainable Design and Educational Environment Research
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• v.2 no.1
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• pp.81-90
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• 2002

The purpose of this study is to create an approach to architectural planning centered on an Biotop-space Factor, with the purpose of applying environmentally friendly architecture to school buildings. For this, the notion of ecological schools is defined, and possible use of the planning standard is studied as a means by which ecological school planning can be carried out. As a result, ecological schools can be viewed as (1) the field of education, (2) the field of logical community and (3) a stronghold of the ecological environment. And The possible creation of a quantified goal and level at Ecological school planning is also reviewed with the Biotop-space Factor.

• Environmental Engineering Research
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• v.18 no.4
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• pp.241-246
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• 2013

For effective and efficient environmental management, developed countries, such as the Netherlands, UK, Australia, Canada, and United States apply ecological risk assessment, and they have an autonomous risk assessment methodology to protect native receptors. In this study, soil ecological protective concentration (EPC) of cadmium in Korea was derived using Korean ecological risk assessment methodology. The soil EPC of cadmium was calculated using probabilistic ecological risk assessment based on species sensitivity distribution. The soil EPC was calculated according to land use for residential/agricultural and industrial/commercial purposes. The chronic soil EPCs for residential/agricultural and industrial/commercial lands were derived to be 1.58 and 9.60 mg/kg, respectively. These values were similar to soil EPC of European Commission, the Netherlands, UK, and Canada. However, these values were lower than the established Korean soil standard, because the current soil standard was based on human risk. Therefore, the impact on an ecosystem when establishing environmental standard should be considered.

• KIEAE Journal
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• v.7 no.2
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• pp.25-30
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• 2007

The purpose of this research is to analyze the water quantity of the rainwater detention system based on the Planning Simulation in Residential Areas. The contents of this research consists of two main parts. The first part is to calculate the supply water quantity of the rainwater detention system and the demand water quantity of the Wonheungs' ecological park. The second part is to analyze the difference between demand and supply of water quantity, based on the Planning Simulation, in the rainwater detention system. This research will contribute to the establishment of the environment-friendly site planning methods which increase the quality of residential environment in apartment housing.