• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.68-73
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• 2011

The single cell gel electrophoresis (SCGE) assay is a microelectrophoretic technique for assessments of DNA damage at the level of the individual eukaryotic cell. The SCGE assay, due to its simplicity, sensitivity and need of a few cells, has advantages compared to other genomic damage assays such as sister chromatid exchange, chromosomal aberration and micronucleus test. In this study, investigated were the levels of DNA damage and the repair kinetics in the coelomocytes of Eisenia fetida treated with HgCl2 and ionizing radiation by means of the SCGE assay. For detecting DNA damage and repair in coelomocytes, earthworms (E. fetida) were irradiated with six doses of ${\gamma}$-rays (0, 2.5, 5, 10, 20 and 50 Gy) and in vivo exposed to mercuric chloride at 0, 80 and 160 mg $kg^{-1}$ for 48 hours. Then the Olive tail moments were measured during 0~12 hours after irradiation and 0~72 hours after Hg treatment. The results showed that the more the oxidative stress was induced by mercury and radiation, the longer the repair time was required. Also, the results suggest that the SCGE assay may be used as an important tool for comparison of the sensitivity of different species to oxidative stresses.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.591-594
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• 2009

Acute and massive death was noted in 10-week-old chickens, broiler breeder, housed in the floor pens. The number of dead chickens exceeded 20 birds each day. Grossly, fibrinous peritonitis with adhesion of mesenteries and intestinal organs was noted. The ceca were enlarged, expanded, and thickened with congestion. Cecal lumen was distended with a caseous core composed of serous, fibrinopurulent, and hemorrhagic exudates with desquamated masses of epithelial cells. The liver had multifocal white irregular necrotic foci surrounded by a raised ring. Light and electron microscope revealed Histomonas meleagridis in the liver with its characteristic structures and not in the intestinal mucoca and submucosa. In this case, the examination of parasite, larvae and egg was conducted more carefully; however, we could not find eggs or worms of Heterakis gallinarum in the dead or live chickens and earthworms in the soils of floor pens. Therefore, we concluded that an outbreak of blackhead disease probably occurred by direct transmission of histomonads from chickens to chickens in this case.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.8
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• pp.607-612
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• 2013

Soil ecological risk assessment requires terrestrial toxicity data based on trophic levels including plants, earthworms, nematodes, and springtails. To expand the trophic levels, it is needed to consider primary producer algae, nearly distributed in terrestrial environment, as representative terrestrial test species. In this study, we collected research cases focused on soil-related test species and exposure media from SCI papers, and analyzed exposure media, test species, test chemicals, and other test methods, for reviewing research trends of soil-related algal toxicity. Up to now, in the soil-related algal toxicity, test species were 8 cases (Pseudokirchneriella subcapitata, Chlorella vulgaris, Scenedesmus bijugatus, Chlorococcum infusionum, Scenedesmus subspicatus, Nostoc linckia, Synechococcus elongatus, and Chlorococcum sp.) and endpoints were cell count or photosynthetic pigment content. Also, 5 of exposure media were liquid medium, soil extracts, porewater, agar medium, and soil. Most of papers used algae isolated from natural soils or soil extracts. There were only one case for assessing algal toxicity in soil medium. More researches regarding algal toxicity in soil environments need to be conducted consistently.

• Resources Recycling
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• v.7 no.3
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• pp.52-57
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• 1998

The objective of this study was to investigate the effect of cow manure as additive material on the ripening of sewage s sludge for vermistabiJization. The changes of the waste properties by ripening for 50days were observed as a function of the v various mixture ratios of sewage sludge ‘and cow manure. The pH values of the mixture wastes decreased from 7.5-7.67 to 6_ 9~7.2 by the ripening for 50days, and the mixture ratio made dIfferent pH values. The initial value of oxidation-reduction p potential (Eh) of the mixture waste was a negative (-) value indicating an unfavorable condition for earthworm after, but the v values of Eh increased with the opening time. The Increase rate at Eh value was prop$\alpha$rtional to the mixture ratio of cow m manure. The value of alkalinity was also changed into the favorable range for earthworm after 50days except for non-use of 1 the cow manure When the mixture ratio of the cow manure increased from 10% to 30%, the growth of earthworms increased h from 63.7% to 88.3 % tor the survival rate, 265% to 321% for the liveweight increasing rate and 66.7_7% to 91% for hatching f rate of the cocoons. It can be concluded that the proper content of tbe cow manure in the sewage sludge to ensure effective v vermistabilization was over 20%, when the mixture was ripened during 50 days. The quantity of ingestion and 며ectian at 20%-30% was found to be O.15--i.L18g sludge and 0 1l--O.14g solid per capacity earthworm per day, respectively.

• Weed & Turfgrass Science
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• v.5 no.3
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• pp.155-164
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• 2016

Earworm is very useful animal in soil ecosystem, however it is harmful for golf courses because they introduce many cast on turfgrass that reduces turf uniformity and play quality. However, no information has found on earthworm diversity and seasonal fluctuation in different Korean golf courses. In this study, we focused to carry out earthworm species composition and seasonal population structure in turfgrass of golf courses. During spring and fall season survey with direct digging and tea saponin drenching sampling in 5 different golf courses, 6 species under 3 families of earthworms were collected. Earthworm species composition and density was different among the golf courses. Aporrectodea caliginosa in Lumbricidae was dominant species in Anseong and Dongrae Benest Golf Club; however Amynthas carnosus in Megascolecidae was dominant species in Anyang and Glenrose Golf Club. Ap. caliginosa was collected only aclitellum in July and Am. carnosus was collected aclitellum and clitellum in August in golf courses. Seasonal population of earthworm was different depending on earthworm species (Am. hupeiensis was the highest in August and Ap. caliginosa was in April) however small number of collected earthworm species were not dominant trend in golf courses.

• Journal of Animal Environmental Science
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• v.18 no.2
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• pp.63-74
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• 2012

This experiment was carried out to investigate the effect of different stocking rate on growth, cast production and conversion efficiency of organic matter to tissues of earthworm. The carbon and nitrogen ratio (C/N) of tested Korean cow manure was 25.1, it was estimated an adequate ratio as feed for earthworms. The different stocking rates were 1:8(S-1), 1:16(S-2), 1:32(S-3) 1:64(S-4) 1:128(S-5) and 1:256 (S-6) as the ratios of earthworm fresh weight to biomass of Korean cow manure, respectively. A stocking rate of 1:32(S-3) was obtained a significantly highest values of increasing rate and conversion efficiency of organic matter to earthworm tissues. The mean values of increasin g rate of fresh weight and conversion efficiency of organic matter to earthworm tissues were 10.63 mg/day and 6.65% at the ratio of 1:32(S-3) with a rearing volume was $56.6cm^3$. A stocking rate of 1:8(S-1) was obtained a highest ratio of vermicasts, but showed a negative values of increasing rate and conversion efficiency of organic matter to earthworm tissues, it may due to severely food competition between individuals during the rearing periods. The pH, total nitrogen, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with stocking rate. Especially, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with rearing progressed. Vermicasts have the potential for improving plant growth when amended to container medium and soil according to increased availability of nutrients and improved physicochemical properties.

• Journal of the Korean GEO-environmental Society
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• v.25 no.5
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• pp.5-13
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• 2024

This paper investigates the stabilization feasibility of contaminated sediment contaminated with benzyl butyl phthalate (BBP) using acid/base-modified activated carbon. The efficiency of stabilizers was evaluated by analyzing the impact of the activated carbon on the decomposition and adsorption of the contaminant, along with the biological effects on earthworms. Additionally, the contaminant migration was monitored with the BBP concentration in pore water using low-density polyethylene. The research results indicated that the accumulated concentration of BBP was approximately 2% lower in the experimental group applying a 5% mixture ratio of modified activated carbon compared to the group applying a 10% mixture ratio. The leaching into water was reduced by over 18% in all experimental conditions after 7-day exposure period. Over 25% reduction was observed after 28-day exposure. The pore water concentrations were measured. After 7 days of exposure, the mechanically mixed experimental group exhibited a higher pore water stabilization rate compared to the biologically mixed group. Within the mechanically mixed group, the experimental group with 10% mixture of modified activated carbon showed a 1% higher stabilization rate than the group with 5% mixture. After 28 days of exposure, the biologically mixed experimental group demonstrated a higher pore water stabilization rate compared to the mechanically mixed group. Moreover, within the biologically mixed group, the experimental group with 10% mixture of modified activated carbon showed approximately 0.1% higher stabilization rate than the group with 5% mixture.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.76-83
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• 2006

In order to investigate the impacts of non-selective herbicide, glufosinate-ammonium (ammonium 4-[hydroxy(methyl)phosphinoyl] -DL-homoalaninate, GLA) to the non-target organisms, earthworm was exposed to GLA in the field soil for a month, and microbial populations in the soil were investigated after application of GLA. Simultaneously, the residues of GLA and its metabolite, 3-MPP were analyzed in the same soil. Meanwhile, to elucidate the influence of GLA to the growth of non-target crops incase of inter-furrow application, the amounts of carotenoid, chlorophyll, amino acid, proteins and sugars in the leaves of potato and chinese cabbage grown in the same field were investigated. In result, the dead earthworm was not observed during the test period, and the increasing rates of bodyweight were $9.410{\sim}11.603%$ in GLA-treated plots and 5.645% in GLA-untreated plots. The populations of fungi, bacteria and actinomycetes in the GLA-treated soils were $6.2{\times}10^4$, $1.5{\times}10^6$ and $5.7{\times}10^4$, respectively. They maintained relatively similar levels to the control which were $3.7{\times}10^4$, $3.7{\times}10^5$ and $3.7{\times}10^4$, respectively. In residue analysis, the limit of detection of GLA was 0.02 mg $kg^{-1}$, that of 3-MPP was the same level, and the half-life of GLA was 15 days in sandy clay loam soil. This result indicates that GLA was degraded very quickly in field soil. On the other hand, the amounts of physiological, biochemical components such as carotenoid, amino acid, chlorophyll, protein and sugar were ranged from 90.0 to 104.3% in potato and from 99.0 to 112.7% in chinese cabbage. Comparing with hand-weeded plots, it is indicated that GLA had not affected to the growth of non-target crops when applied at inter-furrow in crops-growing field.