• 대한임상검사과학회지
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• 제36권2호
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• pp.210-214
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• 2004

This study was carried out to predict the value of serum ${\beta}$ subunit of humans chorionic gonadotropin(${\beta}$- hCG) in early pregnancy viability. This was performed among 85 women in vitro fertilization and embryo transfer(IVF-ET). The serum ${\beta}$-hCG levels were established for 30 normal singleton pregnancies, 10 twin and triplet pregnancies, 10 preclinical abortions, 10 clinical abortions, 20 biochemical abortions and 5 ectopic pregnancies. In comparison to normal singleton pregnancies, multiple pregnancies showed higher ${\beta}$-hCG. But clinical abortions, preclinical abortions and ectopic pregnancies showed lower ${\beta}$-hCG levels than singleton pregnancies. In conclusion, if we predict the value of serum ${\beta}$-hCG of variable early pregnancies and analyze it, we could predict the dilution protocol. Also, it can be useful in other ways.

• 한국건축시공학회지
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• 제12권6호
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• pp.566-574
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• 2012

To address the problem of global warming, consumption of cement, the main material of concrete, should be decreased. Unfortunately, when industrial by-products are used in large quantities as admixture, the early age strength of concrete will be decreased, reducing its viability for use in concrete structures. Therefore, in this study, the application of an ionization accelerator and alkaline activator as addition agent of superplasticizer were investigated to secure a similar early age strength to that of normal concrete, thus increasing the viability of low cement concrete. Through the investigation, it was found that specimens that used a combination of Alkaline-activator (Na2Sio3) and ionization accelerator (Amine) had the highest early and long-age compressive strength. From this, we can determine an appropriate range of application of superplasticizer to improve early-age compressive strength of low cement concrete.

• 한국수정란이식학회지
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• 제15권3호
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• pp.219-230
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• 2000

This study was carried out to determine the factors on achieving good viability of embryos biopsied fur sexing, to investigate pregnancy rate following embryo transfer(ET) with sexed embryos, and to confirm the accuracy for the calves bort following ET with sexed embryos by polymerase chain reaction(PCR). To investigate viability of Hanwoo embryos after biopsy for sexing, fresh and frozen/thawed embryos were biopsied according to different developmental day of blastocysts, different stage of blastocysts, and different biopsy grade and the embryos themselves were incubated for 2 hours in TCM199 after microsection to be evaluated morphologically for recovery as blastocyst. The results obtained were as follows : 1. The rate of oocytes cleaved in vitro and the rate of blastocyst of the cleaved oocytes were 52.5% and 21.6%, respectively. The rate of blastocyst on day 8 was 11.2%, denoting the highest rate during whole culture period posterior to in vitro fertilization(IVF) 2. After biopsy for sexing, the viability rate of blastocyst on day 7, 8 and 9 was 75.0%, 88.4%, and 100.0%, respectively and the viability of early, mid, and expanded blastocyst after biopsy was 75.0%, 88.9%, and 91.1%, respectively The viability rate of fresh and frozen/thawed embryos was 89.9%, 71.4%, respectively. And the viability of expanded, hatching, and hatched blastocyst of frozen/thawed embryos was : 75.0%, 75.0%, and 50.0%, respectively. The viability of embryos according to biopsy grade of 10∼20%, 21∼30%, and 31∼40% was 85.7%, 91.5%, and 71.4%, respectively. 3. Pregnancy rate after transfer with biopsied embryo between flesh and frozen/thawed embryos was 22.6% and 20.0%, respectively. 4. In comparison between sex by PCR method and sex of calves born after embryo transfer, the accuracy of sex deterimination was 92.3% (12/13).

• 한국양식학회지
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• 제20권3호
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• pp.190-193
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• 2007

대농갱이, Leiocassis ussuriensis의 유도된 자성발생성 이배체의 초기 생존율, 초기 성장 및 성비를 분석하였다. 대농갱이 자성발생성 이배체는 일반 대농갱이 이배체 대조군과 비교 시 부화 180일까지 생존 및 성장 형질에서 모두 차이를 나타내지 않았다(P>0.05). 자성발생성 이배체는 부화 10주째 암수 판별이 용이한 수준의 난모세포 발달 정도를 보였고 일반 대농갱이 암컷과 난소 발달에 있어 유사하였다. 부화 10주째 생식소 조직학적 분석 결과 일반 대농갱이 이배체 자손들은 1:1의 암수 비율을 나타낸 반면, 자성발생성 이배체 자손들은 모두 암컷으로 판별됨으로써 본 어종의 성 결정 기구는 XX-XY를 기본으로 하는 암컷 동형접합형인 것으로 나타났다.

• 한국어류학회지
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• 제22권1호
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• pp.25-33
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• 2010

광염성 송사리 Oryzias dancena를 대상으로 서로 다른 염분도 조건이 산란, 발생 및 초기 생존 형질에 미치는 영향을 평가하고자 하였다. 송사리는 본 연구에서 설정한 모든 염분도 조건 (0~70$^{\circ}/_{\circ\circ}$)에서 산란이 가능하였으나 암컷의 산란 참여 빈도 및 산란 주기는 염분도 별로 차이가 관찰되어 담수 (0$^{\circ}/_{\circ\circ}$) 및 70$^{\circ}/_{\circ\circ}$에서 다른 염분도 그룹 (10~50$^{\circ}/_{\circ\circ}$)에 비해 낮은 값을 나타내었다. 조사한 염분도 조건 중 0~30$^{\circ}/_{\circ\circ}$ 구간에서만 수정률의 저하가 없었으며 염분도가 그 이상 높아질 경우 수정률의 유의적인 감소가 관찰되었다. O. dancena 수정란은 본 연구의 0, 15, 30, 45 및 60$^{\circ}/_{\circ\circ}$ 부화조건에서 모두 발생이 가능하였으나 염분도가 상승 할수록 배(embryo)의 생존율과 부화율이 저하되는 경향을 나타내었고, 특히 고염분의 조건에서는 기형 유발과 부화의 지연이 관찰되었다. 부화 자어 대상으로 다양한 염분도 조건에서 부화 후 7 일째까지의 초기 생존율을 관찰한 결과, 45$^{\circ}/_{\circ\circ}$ 이상의 염분도에서 초기 발달 과정 중 유의적인 폐사가 관찰되었으나 이후 안정화되는 경향을 나타내었다.

• 한국양식학회지
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• 제22권1호
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• pp.74-78
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• 2009

동자개(PF) 암컷과 대농갱이(LU) 수컷에 의해 유도된 잡종(FU)을 대상으로 동자개 및 대농갱이 암컷과 역교배체(PFU, LFU)를 유도하여 수정률, 부화율, 초기생존을, 염색체 수 및 핵형분석을 수행하였다. 유도된 PFU ($PF{\times}FU$)의 수정률은 86.8%, 부화율은 70.7% 및 생존율은 76.4%로 양친으로 사용된 PF 및 LU보다 유의적으로 높게 나타났다. 그러나 FU를 사용한 LFU ($PF{\times}FU$)는 모두 사망하였다. PF (2n=52)와 FU(2n=52)간의 PFU의 염색체 수는 2n = 52로 양친과 동일하게 나타났으며 핵형분석 결과 22 metacentrics + 20 submetacentric + 10 acrocentric으로 구성되어 있었다.

• 대한수의학회지
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• 제29권2호
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• pp.123-128
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• 1989

Demi-embryos were successfully produced by bisection of ICR mouse embryos at preimplantation stages. They were microsurgically bisected using a microsurgical blade attached to a micromanipulator after pretreatment with 0.5% pronase in PBS for two minutes or not. Embryos with softened zona pellucida were more easily bisected and less damaged than intact embryos. The highest success rate in bisection has been achieved by selecting blastocysts(94.1% in success rate with intact blastocysts and 100% in success rate with zona softened blastocysts). Demi-embryos without zona pellucida were cultured in D-PBS or M-16 medium at $37^{\circ}C$, 5% $CO_2$ in air for 72 hours for 2-cell stage embryos, 48 hours for 4-to 8-cell stage embryos, 24 hours for morula stage embryos and 6~12 hours for blastocyst stage embryos. For the in vitro culture of 2-cell stage embryos, $100{\mu}M$ 2Na-EDTA was added to the media. M-16 medium was better for the in vitro development of mouse embryos than PBS, and PBS is not considered to be suitable for long-term culture of embryos, especially at early stage of cleavage. In M-16 medium, developing rate of demi-embryos of which pair underwent development to form eublastocysts was 15.8% at 2-cell stage, 16.8% at 4-cell stage, 38% at 8-cell stage, 89.6% at morula stage and 94.4% at blastocyst stage, respectively. The more rapid and efficient production of demi-embryos and higher viability after bisection can be expected by softening zona pellucida with pronase and by selecting morulae or blastocysts rather than embryos at early stage of cleavage.

• Journal of Korean Neurosurgical Society
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• 제55권3호
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• pp.125-130
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• 2014

Objective : This study was conducted to elucidate neuroprotective effect of carnosine in early stage of stroke. Methods : Early stage of rodent stroke model and neuroblastoma chemical hypoxia model was established by middle cerebral artery occlusion and antimycin A. Neuroprotective effect of carnosine was investigated with 100, 250, and 500 mg of carnosine treatment. And antioxidant expression was analyzed by enzyme linked immunosorbent assay (ELISA) and western blot in brain and blood. Results : Intraperitoneal injection of 500 mg carnosine induced significant decrease of infarct volume and expansion of penumbra (p<0.05). The expression of superoxide dismutase (SOD) showed significant increase than in saline group in blood and brain (p<0.05). In the analysis of chemical hypoxia, carnosine induced increase of neuronal cell viability and decrease of reactive oxygen species (ROS) production. Conclusion : Carnosine has neuroprotective property which was related to antioxidant capacity in early stage of stroke. And, the oxidative stress should be considered one of major factor in early ischemic stroke.

• 한국환경과학회지
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• 제24권12호
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• pp.1673-1679
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• 2015

This study was conducted to investigate the effects of fruit set internode on the germination of watermelon seeds. Generally fruits setted in higher than 20 internode were high percent of germination, fruit setted in low internode(5-10) is low. The higher fruit-set internode, fruit weight was significantly increased, but 1,000 seed weight was decreased the more fruit set internode. However number of seed was about 300 seeds irrespective of fruit-set internode. Investigating viability of seed by BP test, general percent germination was recorded highest at 5 flower cluster above 20 internode of fruit-set internode. In contrast, condition of fruit setted on 3, 4, 6 flower cluster were high percent of germination, regarded as unnormal germination not to be as normal seedling by 2~3%. Seed harvested at 5 flower-cluster had high viability on hypocotyl height and diameter of seedling through early growth test, but were not significant. Therefore 5 flower-cluster was optimum fruit-set internode to obtain high-quality seed.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.457-462
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• 2020

Pancreatic cancer is among the most difficult-to-treat tumors. More than half of patients with this cancer have very few symptoms at the early stages, allowing the development of distant metastases and resistance to cancer treatment. In this study, we found that Juniperus chinensis extract (JCX) decreased the cell viability and migration activity of PANC-1 and SNU-213 pancreatic cancer cells in a dose-dependent manner. JCX increased caspase-3 activation and generation of reactive oxygen species (ROS). N-acetylcysteine treatment blocked JCX-induced ROS generation and the negative effects on pancreatic cancer cell viability. In addition, JCX down-regulated the levels of phospho-focal adhesion kinase (p-FAK) and phospho-extracellular signal-regulated kinase (p-ERK). Together, these results indicate that JCX induces apoptosis in human pancreatic cancer cell lines through ROS production, downregulating FAK/ERK signaling and activating caspase-3. We propose that JCX-derived compounds represent candidates for the development of alternative medicines for the treatment of pancreatic cancer.