• Title/Summary/Keyword: Early stress

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Screening Differential Expressions of Defense-related Responses in Cold-treated 'Kyoho' and 'Campbell Early' Grapevines

  • Ahn, Soon Young;Kim, Seon Ae;Han, Jae Hyun;Kim, Seung Heui;Yun, Hae Keun
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.275-281
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    • 2013
  • Low temperature is one of the major environmental factors that affect productivity including reduced growth and budding of vines, and changes of metabolic processes in grape (Vitis spp.). To screen the specific expression of abiotic stress-related genes against cold treatment in 'Kyoho' and 'Campbell Early' grapevines, expression of various defense-related genes was investigated by RT-PCR and real-time PCR. Among the 67 genes analyzed by RT-PCR and real-time PCR, 17 and 16 types of cDNA were up-regulated, while 5 and 6 types were down-regulated in cold-treated 'Kyoho' and 'Campbell Early' grapevines, respectively. Genes encoding carotene (Cart3564 and Cart4472), chalcone isomerase (CHI), cytochrome P450 (CYP), flavonol synthase (FLS), endo-${\beta}$-glucanase precursor (Glu), glutathione peroxidase (GPX), glutathione-S-transferase (GST), leucine-rich repeats (LRR), manganese superoxide dismutase (Mn-SOD), phenylalanine ammonia lyase (PAL), polygalacturonase-inhibiting protein (PGIP), proline rich protein 2 (PRP2), small heat shock protein (sHSP), temperature induced lipocalin (TIL), and thaumatin-like protein (TLP) were up-regulated, while those encoding CBF like transcription factor (CBF1), chitinase-like protein (CLP), cold induced protein (CIP), glycerol-3-phosphate acyltransferase (GPAT), and mitogen-activated protein kinase (MAPK) were down-regulated by low temperature treatment in both in 'Kyoho' and 'Campbell Early'.

Prevalence of Posttraumatic Stress Disorder Among North Korean Defectors During Preparation for Early Settlement

  • Chang, Seung-Yong;Jeon, Jin-Yong;Maeng, Se-Ri;Kim, Hyeyoung;Bae, Jae-Nam;Lee, Jung-Sub;Kim, Won-Hyoung
    • Anxiety and mood
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    • v.17 no.2
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    • pp.67-72
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    • 2021
  • Objective : This study examined the prevalence of posttraumatic stress disorder (PTSD) among North Korean defectors during preparation for early settlement. In addition, the correlation between PTSD and other mental disorders with suicidal ideation was assessed. Methods : A total of 639 North Korean defectors aged 19-65 years were included from resettlement and training centers. PTSD was assessed using the PTSD Diagnostic Scale (PDS). The prevalence of other mental disorders and suicidal ideation were assessed using the Korean version of the Psychiatric Diagnostic Screening Questionnaire (K-PDSQ). Results : A total of 6.8% of North Korean defectors had a PDS score >23, which was suggestive of a diagnosis of PTSD. Prior repatriation to North Korea was significantly associated with PTSD. The defectors with PTSD had a higher prevalence of other mental disorders (major depressive disorder prevalence: adjusted odds ratio [AOR], 14.5; 95% confidence interval [CI], 6.7-31.2, panic disorder prevalence: AOR, 9.5; 95% CI, 4.6-20.0, generalized anxiety disorder prevalence: AOR, 5.6; 95% CI, 2.4-13.2). No significant association was found between suicidal ideation and PTSD. Conclusion : North Korean defectors had a relatively high prevalence of PTSD and suicidal ideation during preparation for early settlement. Cohort studies are needed to assess the lasting effects of PTSD on North Korean defectors' settlement in Korea for a longer period.

The Effect of Fatigue Fracture in shot peening Marine structural steel at stress ratio (쇼트피닝 가공된 해양구조용강의 피로파괴에 미치는 응력비의 영향)

  • Park, Kyoung-Dong;Han, Kun-Mo;Jin, Young-Beom
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2003.10a
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    • pp.138-144
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    • 2003
  • Rencentely, the request for the light weight is more incresed in the area of industrial environment and machinery and consistent effort is needed to accomplish high strength of material for the direction of light weight. we got the following characteristic from crack growth test carried out in the range of stress ration of 0.1, 0.3 and 0.6 by means of opening mode displacement. At the content stress ratio, the threshold stress intensity factor crack range ${\Delta}K_{th}$in the early stage of fatigue crack growth (Region I) and dtress intensity factor range ${\Delta}K$ in the stable of fatigue crack growth (Region II) with an increase in ${\Delta}K$. Fatigue life shows more improvement in the Shot-peened material than in the Un-peening material. And compressive residual stress of surface on the Shot peening processed operate resistance force of fatigue. So we can obtain fallowings. (1) The fatigue crack growth rate on stage II is conspicuous with the size of compressive residual stress and is depend on Paris equation. (2) Although the maxium compressive residual stress is deeply and widely formed from surface, fatigue life does not improve than when maxium compressive residual stress is formed in surface. (3) The threshold stress intensity factor range is increased with increasing compressive residual stress.

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Assessment of Incipient Decay of Radiata Pine Wood Using Stress-wave Technique in the Transverse Direction (횡단방향(橫斷方向) 응력파(應力波) 방법(方法)에 의(依)한 라디에타소나무의 초기부후(初期腐朽) 평가(評價))

  • Kim, Gyu-Hyeok;Jee, Woo-Guen;Ra, Jong-Bum
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.3
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    • pp.18-27
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    • 1996
  • The feasibility of using stress-wave technique in the transverse direction for the assessment of early stages of decay was investigated using compression test specimens having different annual ring orientations subjected to decay by Tyromyces palustris for various time intervals. Decay detection, quantitative assessment of decay, and the prediction of residual strength of decayed wood with less than five percent weight loss can be feasible using stress-wave parameters (wave velocity, wave impedance, and stress-wave elasticity) and their percent reduction due to decay, measured by stress-wave technique in the transverse direction. The use of stress-wave technique in the transverse direction for the application of this technique to structural members in service is desirable, when considering the easiness of attachment of accelerometers of stress-wave measuring device on the surface of members and also accurate detection of localized decayed areas. In stress-wave technique in the transverse direction, stress-wave parameters measured were different according to the angles between wave propagation path and annual ring, due to the anisotropy of wood structure. Therefore, it is recommended to use percent reduction in stress-wave parameters instead of stress-wave parameters. This evaluation method using percent reduction in stress-wave parameters is ideal when it is impossible to observe annual ring orientation on the transverse surface of wood.

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Functional Implications of Transporters Under Nitrosative Stress Conditions

  • Yu, Kyung-Ha;Maeng, Han-Joo;Chung, Suk-Jae
    • Journal of Pharmaceutical Investigation
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    • v.40 no.3
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    • pp.139-153
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    • 2010
  • Nitrosative stress is defined as pathophysiological conditions that are related to covalent modifications of proteins by nitration/nitrosylation by forms of nitrogen oxide ($NO_x$), leading to DNA damage, ultimately, cell death. This type of stress condition appears to be associated with a number of disease states, including diabetes, inflammation and neurodegenerative diseases. Since these pathological conditions are frequently chronic in nature and, thus, require long-term treatment, changes in pharmacokinetics are likely to affect the therapy. Transporters are membrane proteins that facilitate the movement of substrates, including drugs, across plasma membranes of epithelial / endothelial cells. Since it is now increasingly evident that transporters are pharmacokinetically significant, functional alteration of transporters by this stress condition may have therapeutic relevance. In this review, experimental techniques that are used to study both in vivo and in vitro nitrosative stress are summarized and discussed, along with available literature information on the functional implication of transporters under conditions of nitrosative stress conditions. In the literature, both functional induction and impa irment were apparently present for both drug transporter families [i.e., ATP-binding cassette (ABC) and solute carrier families (SLC)]. Furthermore, a change in the function of a certain transporter appears to have temporal dependency by impairment in the early phase of nitrosative stress and induction thereafter, suggesting that the role of nitrosative stress is complex in terms of functional implications of the transporters. Although the underlying mechanisms for these alterations are not fully understood, protein nitration/nitrosylation appears to be involved in the functional impairment whereas transcript factor(s) activated by nitrosative stress may play a role, at least in part, in functional induction. Interestingly, functional induction under conditions of nitrosative stress has not been observed for SLC transporters while such impairment has been documented for both ABC and SLC transporters. Further investigations appear to be necessary to fully delineate the underlying reasons for these differences on the impact and importance of nitrosative stress conditions.

A Comparative Study of Job Satisfaction and Stress Coping Strategies of Teachers in Early Childhood Education and Care According to Their Personality Types (영유아교사의 성격유형에 따른 직무만족도와 스트레스 대처 방식의 차이)

  • Chae, Jin Young;Kwon, Hye Jin
    • Korean Journal of Childcare and Education
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    • v.8 no.3
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    • pp.5-23
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    • 2012
  • The purpose of this study was to investigate and compare how the Enneargram personality types of teachers in early childhood education and care would affect their job satisfaction and stress coping strategies. The findings are as follows. First, as to the distribution of the participants' Enneargram personality types, the most common was the Peacemaker type and the least common was the Achiever type. Second, for teachers' job satisfaction, there was a statistically significant difference in the job-related subcategory according to their personality types. The post-hot comparison showed that the Loyalist type showed the lowest score in job-related job satisfaction. Third, in terms of stress coping strategies, there was a statistically significant difference in the psychological coping strategy according to their personality types. The post-hot comparison showed that the Individualist type showed the highest score in psychological coping strategy. The implications for future studies are also discussed.

Amelioration of 1,2 Dimethylhydrazine (DMH) Induced Colon Oxidative Stress, Inflammation and Tumor Promotion Response by Tannic Acid in Wistar Rats

  • Hamiza, Oday O.;Rehman, Muneeb U.;Tahir, Mir;Khan, Rehan;Khan, Abdul Quaiyoom;Lateef, Abdul;Ali, Farrah;Sultana, Sarwat
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.9
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    • pp.4393-4402
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    • 2012
  • Colon cancer is the third most common malignant neoplasm in the world and it remains an important cause of death, especially in western countries. The toxic environmental pollutant, 1, 2-dimethylhydrazine (DMH), is also a colon-specific carcinogen. Tannic acid (TA) is reported to be effective against various types of chemically induced toxicity and also carcinogenesis. In the present study, we evaluated the chemopreventive efficacy of TA against DMH induced colon toxicity in a rat model. Efficacy of TA against the colon toxicity was evaluated in terms of biochemical estimation of antioxidant enzyme activities, lipid peroxidation, histopathological changes and expression of early molecular markers of inflammation and tumor promotion. DMH treatment induced oxidative stress enzymes (p<0.001) and an early inflammatory and tumor promotion response in the colons of Wistar rats. TA treatment prevented deteriorative effects induced by DMH through a protective mechanism that involved reduction of oxidative stress as well as COX-2, i-NOS, PCNA protein expression levels and TNF-${\alpha}$ (p<0.001) release. It could be concluded from our results that TA markedly protects against chemically induced colon toxicity and acts plausibly by virtue of its antioxidant, anti-inflammatory and antiproliferative activities.

The Influence of Teacher Efficacy and Stress Coping Strategies on Job Satisfaction of Teachers in Early Childhood Education and Care (영유아교사의 교사효능감과 스트레스 대처방식이 직무만족도에 미치는 영향)

  • Chae, Jin-Young
    • Korean Journal of Childcare and Education
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    • v.9 no.6
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    • pp.223-241
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    • 2013
  • The purpose of this study was to examine the influence of teacher efficacy and stress coping strategies on job satisfaction of teachers in early childhood education and care. 189 teachers were recruited from 8 day care centers and 10 kindergartens in Seoul, Kyunggi, Kyungbuk, and Chonbuk provinces in Korea. Data were analyzed through frequencies, Pearson's correlations, and the stepwise regression analyses using PASW 18.0. The findings are as follows. First, the result showed that teachers in ECEC were most likely to be satisfied with their job characteristics most among the 4 subcategories in job satisfaction. They also perceived their the ability of discipline high for teacher efficacy, and used the direct coping strategy most as a stress coping strategy. Second, the teachers' ability to create a positive learning atmosphere affected job characteristics, the ability to participate in decision-making affected the relationship with director and colleagues, working environment, welfare and salary the most. Third, the direct coping strategy had the most impacts on all subcategories of job satisfaction. The results of this study may provide basic data for finding effective way to increase job satisfaction of teachers in ECEC.

The Effects of the Hot, Humid Tropical Climate and Early Age Feed Restriction on Stress and Fear Responses, and Performance in Broiler Chickens

  • Al-Aqil, A.;Zulkifli, I.;Sazili, A.Q.;Omar, A.R.;Rajion, M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.11
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    • pp.1581-1586
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    • 2009
  • The present study was conducted to determine the effects of two types of housing systems and early age feed restriction on stress and fear reactions, and performance in broiler chickens raised in a hot, humid tropical climate. On day 1, chicks were housed either in windowless environmentally controlled chambers (temperature was set at 32$^{\circ}C$ on day 1 and gradually reduced to 23$^{\circ}C$ by day 21) or in conventional open-sided houses (OH) with cyclic temperatures (minimum, 24$^{\circ}C$; maximum, 34$^{\circ}C$). An equal number of chicks from each housing system was subjected to either ad libitum feeding (AL) or 60% feed restriction on day 4, 5 and 6 (FR). The CH birds showed greater weight gain, higher feed consumption and better feed conversion ratios (FCR) than their OH counterparts. Feeding regimen had negligible effect on overall performance. Neither housing nor feeding regimen had a significant (p<0.05) effect on mortality rate. Although the CH birds were less stressed, as measured by plasma corticosterone concentration (CORT), than those of OH, the former showed longer TI duration suggesting higher magnitude of underlying fearfulness. A significant (p<0.05) effect of housing on heterophil/lymphocyte ratios was only noted among the AL birds where the CH birds had higher values than OH. Collectively, these results suggest that although OH birds had poorer performance and higher level of stress than CH, the former were less fearful. Although FR had negligible effect on growth performance, the regimen alleviated both stress and fear reactions in broilers.

Growth Retardation and Death of Rice Plants Irradiated with Carbon Ion Beams Is Preceded by Very Early Dose- and Time-dependent Gene Expression Changes

  • Rakwal, Randeep;Kimura, Shinzo;Shibato, Junko;Nojima, Kumie;Kim, Yeon-Ki;Nahm, Baek Hie;Jwa, Nam-Soo;Endo, Satoru;Tanaka, Kenichi;Iwahashi, Hitoshi
    • Molecules and Cells
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    • v.25 no.2
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    • pp.272-278
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    • 2008
  • The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.