• Title/Summary/Keyword: Early release

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Effect of Ruthenium Red and Ryanodine on Calcium Ion Metabolism in Oocyte and Early Embryo of Mouse (생쥐의 난자와 초기배아의 칼슘이온 대사에 미치는 Ruthenium Red와 Ryanodine의 영향)

  • Lee Joon Yeong;Hong Soon Cap;Kim Tae Sik;Min Byeong Yeol;Kim Haekwon;Yoon Yong-Dal
    • Development and Reproduction
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    • v.7 no.2
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    • pp.95-103
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    • 2003
  • Intracellular calcium is an important physiological factor in most cells, and ruthenium red and ryanodine play an important role as calcium modulators. Ruthenium red inhibits calcium-induced calcium release(CICR) from the intracellular calcium store. Ryanodine activates calcium release through ryanodine channel. The present experiment was performed to investigate the effects of two modulators on calcium ion metabolism and to determine their dose-dependency in oocyte and early embryo of mouse. Intracellular calcium ion concentration was measured in realtime by using confocal laser scanning microscope(CLSM) after loading of Fluo-3/AM in mouse oocytes and early embryos. Ruthenium red decreased intracellular calcium ion concentration in oocytes and early embryos at its high concentration(30, 300 $\mu$M). Ryanodine increased intracellular calcium ion concentration in oocytes and early embryos in low concentration(0.01 $\mu$M) but decreased that at higher concentrations(1, 10 $\mu$M). These results indicate that two modulators affected calcium ion metabolism in oocyte and early embryo of mouse, and their dose-dependency was different from somatic cell including myocytes.

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Early cicatricial lagophthalmos release with pentagonal wedge resection of the scar, fat redistribution, and full-thickness skin grafting

  • Chung, Chan Min;Tak, Seung Wan;Lim, Hyoseob;Cho, Sang-Hun;Lee, Jong Wook
    • Archives of Craniofacial Surgery
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    • v.21 no.1
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    • pp.49-52
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    • 2020
  • The specialized structure of the upper eyelid ensures complete closure of the eye and eyeball sealing. An upper eyelid injury can cause various symptoms associated with eyeball trauma, not just scar formation or eyelid deformity. In this report, we describe a case of lagophthalmos observed after wound repair in a patient with a crushing injury caused by a grinder. Several surgical techniques are used to treat lagophthalmos or scar contracture. In most cases, a releasing procedure is performed after 6 months of initial repair. However, if the patient has severe symptoms that are not relieved by conservative care, early revision is inevitable. We describe a case of early lagophthalmos successfully resolved with pentagonal wedge resection, fat redistribution, and full-thickness skin grafting. After the revisional surgery, we observed that the patient regained the ability to completely close the injured eyelid, with restoration of function and favorable cosmetic outcomes. Pentagonal wedge resection to release a retracted structure, fat redistribution to prevent readhesion, and full-thickness skin grafting for enough amount of skin to regain upper eyelid function is useful for scar release and lagophthalmos following crushing injuries of the upper eyelid.

Prediction of Dormancy Release and Bud Burst in Korean Grapevine Cultivars Using Daily Temperature Data (기온자료에 근거한 주요 포도품종의 휴면해제 및 발아시기 추정)

  • Kwon Eun-Young;Song Gi-Cheol;Yun Jin I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.3
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    • pp.185-191
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    • 2005
  • An accurate prediction of dormancy release and bud burst in temperate zone fruit trees is indispensable for farmers to plan heating time under partially controlled environments as well as to reduce the risk of frost damage in open fields. A thermal time-based two-step phenological model that originated in Italy was applied to two important grapevine cultivars in Korea for predicting bud-burst dates. The model consists of two sequential periods: a rest period described by chilling requirement and a forcing period described by heating requirement. It requires daily maximum and minimum temperature as an input and calculates daily chill units (chill days in negative sign) until a pre-determined chilling requirement for rest release is met. After the projected rest release date, it adds daily heat units (anti-chill days in positive sign) to the chilling requirement. The date when the sum reaches zero isregarded as the bud-burst in the model. Controlled environment experiments using field sampled twigs of 'Campbell Early' and 'Kyoho' cultivars were carried out in the vineyard at the National Horticultural Research Institute (NHRI) in Suwon during 2004-2005 to derive the model parameters: threshold temperature for chilling and chilling requirement for breaking dormancy. The model adjusted with the selected parameters was applied to the 1994-2004 daily temperature data obtained from the automated weather station in the NHRI vineyard to estimate bud burst dates of two cultivars and the results were compared with the observed data. The model showed a consistently good performance in predicting the bud burst of 'Campbell Early' and 'Kyoho' cultivars with 2.6 and 2.5 days of root mean squared error, respectively.

Development of Revegetation Methods for Restoration of the Disturbed Slopes -Application on the Seed Attached Ripping Net Revegetation Methods- (급경사 비탈면의 녹화 공법 개발 -종자부착 리핑네트공법을 중심으로-)

  • Kim, Eui-Young;Kim, Nam-Choon;Kang, Jin-Hyung;Bae, Sun-Woo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.4
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    • pp.61-69
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    • 2002
  • This study was conducted to develop revegetation methods for the restoration of the steep slopes by seed attached ripping net revegetation methods. In general, steep slopes with decomposed granite soils and ripping rock are easy to erode by precipitation and impossible to revegetate only using hydroseeding with core net mulching because of poor soil fertility. The Seed Attached Ripping Net Revegetation Methods(SALNRM) will be the most popular and sustainable methods to restore decomposed granite soils and ripping rock exposed slopes. The main results are summarized as follows; 1. The net size with $1.5cm{\times}1.0cm$ density was more suitable for growing plants, and increasing the ratio of the slow release fertilizer was better to make early coverage and to grow germinated plants. 2. The fertilizer bag made by the ratio of Peatmose : Vermiculite : Perlite : Quick release fertilize r : Slow release fertilizer = 10 : 2 : 5 : 3 : 4 (v : v) was the best for plant's growth. 3. According to the seed mixture experiments, even though not using foreign grasses, the SALNRM using native plants can make diverse plant composition. 4. The SALNRM will make same early ground coverage by only using native plants like using foreign grasses. The SALNRM would become the popular revegetation methods to restore decomposed granite soils and ripping rock exposed slopes in Korea.

Integrated Level 1-Level 2 decommissioning probabilistic risk assessment for boiling water reactors

  • Mercurio, Davide;Andersen, Vincent M.;Wagner, Kenneth C.
    • Nuclear Engineering and Technology
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    • v.50 no.5
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    • pp.627-638
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    • 2018
  • This article describes an integrated Level 1-Level 2 probabilistic risk assessment (PRA) methodology to evaluate the radiological risk during postulated accident scenarios initiated during the decommissioning phase of a typical Mark I containment boiling water reactor. The fuel damage scenarios include those initiated while the reactor is permanently shut down, defueled, and the spent fuel is located into the spent fuel storage pool. This article focuses on the integrated Level 1-Level 2 PRA aspects of the analysis, from the beginning of the accident to the radiological release into the environment. The integrated Level 1-Level 2 decommissioning PRA uses event trees and fault trees that assess the accident progression until and after fuel damage. Detailed deterministic severe accident analyses are performed to support the fault tree/event tree development and to provide source term information for the various pieces of the Level 1-Level 2 model. Source terms information is collected from accidents occurring in both the reactor pressure vessel and the spent fuel pool, including simultaneous accidents. The Level 1-Level 2 PRA model evaluates the temporal and physical changes in plant conditions including consideration of major uncertainties. The goal of this article is to provide a methodology framework to perform a decommissioning Probabilistic Risk Assessment (PRA), and an application to a real case study is provided to show the use of the methodology. Results will be derived from the integrated Level 1-Level 2 decommissioning PSA event tree in terms of fuel damage frequency, large release frequency, and large early release frequency, including uncertainties.

Ethylene Release of Panax ginseng in Relation to Plant Part and Various Conditions (고려인삼의 식물부위 및 여러 조건과 관련한 에틸렌가스 방출)

  • Park, Hoon;Lee, Myong-Gu;Lee, Chong-Wha
    • Journal of Ginseng Research
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    • v.14 no.2
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    • pp.122-125
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    • 1990
  • Ethylene was released from leaf and fruit but root of Panax ginseng. Root callus showed higher ethylene release (ER) than fruit ER increased with leaf senesence. Fruit during ripening showed decreasing ER in the order of green stage, early stage of reddening and fully ripened stage. between leaves from the plant with fruits in different stages of ripening showed similar trend of fruit in ER but it was about 10 times higher in leaves than in fruits. Leaves of P. quinquefolius showed about 200 times higher ER than that of P ginseng on 22 July Fruits from the plant treated with ethephon showed higher ER after 109 days. Forty-five day-old seedlings grown with various growth regulators showed a significant decrease of stem length and significant increase of ER only in Uniconazole (0.1 ppm) and H-9 (0.0, 5 ppm) solution.

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Induction of apoptosis in human promyelocytic leukaemia HL -60 cells by yomogin involves release of cytochrome c and activation of caspase

  • Jeong, Seoung-Hee;Koo, Sung-Ja;Ryu, Shi-Yong;Park, Hee-Jun;Lee, Kyung-Tae
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.319.1-319.1
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    • 2002
  • Yomogin. an eudesmane sesquiterpene isolated from Artemisia princeps, was found to induce apoptosis in human promyelocytic leukaemia, HL -60 cell with characteristic apoptotic features like nuclear condensation, apoptotic body formation, flipping of membrane phosphatidylserine, release of mitochondrial cytochrome c and caspase-8. -9. and -3 activation. Furthermore. early yomogin-induced cytochrome c release was not affected by the caspase inhibitor Z-VAd fmk and preceded loss of mitochondrial membrane potential. The results suggest that induction of apoptosis by yomogin may provide a pivotal mechanism for their cancer chemopreventive function.

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Anterior interosseous nerve palsy in the early postoperative period after open capsular release for elbow stiffness: a case report

  • Christopher A. Colasanti;Michael Boin;Jacques Hacquebord;Mandeep S. Virk
    • Clinics in Shoulder and Elbow
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    • v.26 no.4
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    • pp.462-466
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    • 2023
  • Surgical release of elbow contracture is associated with injury to structures traversing the elbow. To date, only one other case report has been published describing anterior interosseous nerve (AIN) palsy that developed immediately after open elbow contracture release and debridement. Here we describe the unique case of a patient that developed AIN palsy 1 week after operation, including magnetic resonance imaging and electrodiagnostic studies, to shed some light on the etiology of this rare complication.