• 제목/요약/키워드: Early development

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Studies of In Vitro Embryo Culture of Guppy (Poecilia reticulata)

  • Liu, LiLi;Lee, Ki-Young
    • 한국발생생물학회지:발생과생식
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    • 제18권3호
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    • pp.139-143
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    • 2014
  • Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studies on their early development of live bearers like the guppy including lineage tracing and genetic manipulations, have been limited. Therefore, to optimize conditions of embryo in vitro culture, explanted embryos from pregnant females were incubated in embryo medium (L-15 medium, supplemented with 5, 10, 15, 20% fetal bovine serum, respectively). We investigated whether the contents of FBS in vitro culture medium impact the development of embryos, and whether they would hatch in vitro. Our study found that in 5% of FBS of the medium, although embryos developed significantly slower in vitro than in the ovary, it was impossible to exactly quantify the developmental delay in culture, due to the obvious spread in developmental stage within each batch of eggs, and embryos can only be maintained until the early-eyed. And although in culture with 20% FBS the embryos can sustain rapid development of early stage, but cannot be cultured for the entire period of their embryonic development and ultimately died. In the medium with 10% and 15% FBS, the embryos seems well developed, even some can continue to grow after follicle ruptures until it can be fed. We also observed that embryonic in these two culture conditions were significantly different in development speed, in 15% it is faster than 10%. But 10% FBS appears to be more optimizing condition than 15% one on development process of embryos and survival rate to larvae stage.

유아교사의 이직의도에 대한 Q 방법론적 분석 (A Study on Turnover Intention of Early Childhood Teachers: Q Methodological Approach)

  • 박봉환;남미경
    • 한국보육지원학회지
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    • 제13권3호
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    • pp.55-81
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    • 2017
  • Objective: The purpose of this study was to classify turnover intention of early childhood teachers. This study also analyzed the characteristics of each types of turnover intentions. The research questions were as follows: First, what are the types of turnover intention among early childhood teachers?. Second, what are the characteristics of the types of turnover intention among early childhood teachers?. Method: This study has adopted the Q methodology as a method. first, open surveys were conducted among 101 early childhood teachers and principals who work at kindergartens or child care centers along with literature research, followed by the establishment of Q samples(48 questions). Second, the Q sample was classified among the P sample and was comprised of 90 participants. Ninety early childhood teachers as P sample sorted the 48 Q statements. Results: The findings of this study were summarized as follows: First, the types of early childhood teacher's turnover intentions were identified as four-factors. Second, the characteristics of the four-types of turnover intention were interpreted as follows: 'The type of complex psychological conflict', 'The type of pursuit of social value', 'The type of dependence on organizational environment', 'The type of realistic self-development'. Conclusion/Implications: We suggested political and institutional supporting ways for each type of early childhood teachers.

VES-LMC의 열 특성을 고려한 자기수축 (Autogenous Shrinkage of VES-LMC considering Hydration-Heat)

  • 최판길;이봉학
    • 산업기술연구
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    • 제25권B호
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    • pp.73-80
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    • 2005
  • Durability of concrete structures is seriously compromised by cracking at early-age concretes, particularly in high-strength or high-performance concrete structures. Since early-age cracking is influenced by various factors that affect the hydration process, early-age shrinkage and stress/strain development, the behavior at early-age is highly complex and no rational methodologies for its control have yet been established. Concrete structures often present volumetrical changes particularly due to thermal and moisture related shrinkages. Volumetric instability is detrimental to the performance and durability of concrete structures because structural elements are usually restrained. These restrained shrinkages develope tensile stresses which often results in cracking in combination with the low fracture resistance of concrete. Early-age defects in high-performance concrete due to thermal and autogenous deformation shorten the life cycle of concrete structures. Thus, it is necessary to examine the behavior of early-age concrete at the stages of design and construction. The purpose of this study was to propose a shrinkage models of VES-LMC (very-early strength latex-modified concrete) at early-age considering thermal deformation and autogenous shrinkage.

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조기교통개방 콘크리트의 내구성능 향상에 관한 연구 (Enhanced Durability Performance of High Early Strength Concrete for Early Traffic Opening)

  • 원종필;김현호;안태송
    • 콘크리트학회논문집
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    • 제13권4호
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    • pp.362-370
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    • 2001
  • 콘크리트포장 보수공사에서 조기교통개방을 위해 현재 국내에서는 초속경시멘트를 사용하고 있으나 이는 초기의 빠른 수화반응과 내.외부적 구속으로 인한 온도수축 및 건조수축이 발생하여 콘크리트포장에 인장응력을 유발한다. 구속으로 인한 수축은 콘크리트내에 균열을 발생시켜 투수성을 증가시키고 장기적으로는 내구성을 약화시킨다. 콘크리트보강재료로서 섬유는 이러한 균열을 제어하는데 효과적이다. 본 연구에서는 국내에서 사용되고 있는 3종류의 초속경시멘트의 내구성을 평가하였고 내구성을 증진시키기 위하여 콘크리트 보강 섬유를 혼입하였으며 일반 콘크리트와 섬유보강 콘크리트를 비교.분석하였다. 실험결과 조기교통개방콘크리트에서 섬유의 혼입은 일반 콘크리트보다 내구성을 증진시키는데 우수한 효과를 나타내었다.

초기재령에서 동결을 받은 고로슬래그 콘크리트의 강도발현특성에 관한 실험적 연구 (An Experimental Study on Strength Properties of Concrete Using Blast-Furnace Slag Subjected to Freezing at Early Age)

  • 최성우;반성수;유득현;최봉주
    • 콘크리트학회논문집
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    • 제15권1호
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    • pp.43-51
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    • 2003
  • 동절기 콘크리트 시공에 있어서 낮은 외기온에 따른 콘크리트의 강도발현이 저하되며, 동결 및 융해에 따른 콘크리트의 내부조직의 이상으로 인해 내구성이 저하하는 것으로 알려져 있다. 따라서 한중콘크리트 제조시 조기강도를 확보하기 위해 혼화재의 사용을 경시하고 있는 실정이다. 본 연구는 한중콘크리트의 제조시 혼화재의 사용에 따른 초기동해의 영향을 평가하기 위한 실험을 실시한 것으로서, 초기동해를 입히기 위한 실험 조건으로서, 본 연구는 초기 재령에서 콘크리트에 영향을 미치는 동결온도 및 동결지속시간, 초기양생시간에 따른 혼화재를 사용한 콘크리트의 강도발현특성을 검토하였으며, 실험조건으로서 초기양생기간을 비빔 직후, 12, 24, 48시간, 동결시간을 12, 24, 48시간, 동결온도는 -1, -10, $-15^{\circ}C$로 설정하여 실험을 실시하였다. 본 연구의 실험결과 고로슬래그 미분말의 대체율이 증가할수록 초기재령에서의 강도발현은 지연되는 것으로 나타났으나, 응결을 고려할 경우 고로슬래그 미분말 대체율 20%까지는 거의 차이가 없는 것으로 나타났다. 또한 동결에 따른 강도발현특성을 살펴보면, 비빔 직후 동결시킨 경우에서는 동결온도와 상관없이 재령 28일에 있어서 동결 받지 않은 경우의 80%를 발현하는 것으로 나타났으나, 12시간 이상 양생을 실시한 후 동결시킨 경우에 있어서는 동결온도 및 동결시간과 상관없이 동결시키지 않은 경우와 거의 유사한 강도를 발현하는 것으로 나타났으며, 모든 실험조건에서 혼화재를 사용한 경우에 있어서는 고로슬래그 미분말에 의한 강도회복효과가 매우 큰 것으로 나타났다. 따라서 동절기 초기재령에서 적절한 양생을 실시한다면, 한중콘크리트 제조시 고로슬래그 및 플라이애시와 같은 혼화재의 사용에 따른 콘크리트의 성능향상 효과가 매우 클 것으로 사료된다.

하전작물 품종 및 재배기술의 1962년 이후 변천 (Changes in Variety and Cultural Practices of Soybean, Sweet Potato and Corn Since 1962 in Korea)

  • 홍은희;박근용
    • 한국작물학회지
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    • 제27권4호
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    • pp.462-469
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    • 1982
  • Since 1962, varietal development and dissemination for summer upland crops have been actively initiated by the Crop Experiment Station, Office of Rural Development, Suweon, Korea. The major breeding objectives of soybeans have been to develop varieties which are early maturing, disease resistant, and adaptable to late planting for after-barley cropping. Development of eleven new soybean varieties including Hwangkeumkong, Jangyeobkong, Danyeobkong, and Kwangkyo has greatly increased the soybean yield throughout country. For com, after development of Hwangok #2, a synthetic, in early 1960's, nine corn hybrids-single crosses, double-crosses, and three-way crosses-such as Jecheon-ok, Hoengseong-ok, Kwangok, Suweon #19, etc., have been disseminated mainly to Kangweon province, a major corn producing area in Korea, and drew up the yield over 4 tons per hectare. The major breeding objectives of sweet potato have been to develop varieties which have high starch content and root yield. Hwangmi, Hongmi, and Shinmi are three sweet potato varieties developed and disseminated by the Crop Experiment Station, Office of Rural Development and are grown most widely in Korea. Most of researches on cultural practices of upland crops have begun on a full scale from early 1960's. In soybeans, for example, no fertilizer but for barley was applied although the effects of phosphate and potassium fertilizers were great on soybeans in after-barley soybean croppings. The effects of heavy application of phosphate and calcium fertilizers on soybeans in newly reclaimed soils were recognized. Recently a mixed fertilizer for soybean (N; 40, P:70, K:60 kg/㏊) was developed and sold for soybean growers. The optimum planting densities of 220, 000 plants/ha in full-season cropping and 330, 000 pts/ha in after-barley cropping of soybeans were known from repeated experiments. For higher yield, a means of cultural practices such as transplanting-pinching, direct planting-pinching, and hilling-up, etc., were developed along with barley-stubble planting with no tillage and integrated herbicide application for labour savings. For sweet potato, cultural practices for planting date, harvesting date, fertilizer, and planting density were fully established. For early marketing, a technique of vinyl-mulching on sweet potato has also fully developed. For com, planting density of 37, 000 pts/ha in early 1960's has been changed to 55, 000 pts/ha for grain production and 67, 000 pts/ha for silage. The amounts of fertilizers have also been changed from 120-120-120kg/ha (N-P-K) in early 1960s to 180-150-150 kg/ha. These increases in number of plants per unit area and fertilizer levels have resulted in greater production for both grain and silage. At the same time, the production techniques of F1 seeds have also improved.

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사용재료 및 양생조건에 따른 콘크리트의 조기강도발현 특성에 관한 실험적 연구 (An Experimental Study on the Early Strength Development Properties of Concrete According to Curing Condition and Used Materials)

  • 이상수;송하영;이지환
    • 콘크리트학회논문집
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    • 제20권6호
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    • pp.721-729
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    • 2008
  • 본 연구는 조기강도 발현용 콘크리트의 유동특성 및 강도특성을 비롯한 공학적 특성을 알아보기 위한 것으로서 실내배합시험에서 각 실험 요인 및 수준을 설정하여 검토한 결과 시멘트의 경우 OPC보다 CHC와 HESPC가 우수한 품질 성능을 나타냈으며, PNS계 혼화제보다 PC계 혼화제가 경시변화 및 조기강도 발현 측면에서 우수하게 나타났다. 또한, 양생 온도에 따라 많은 차이를 나타냈는데, 양생온도 $12^{\circ}C$ 이하의 온도에서는 조기강도 발현에 상당한 취약성을 나타냈으며 콘크리트 종결 직후의 양생온도에 의해 조기강도 발현에 상당한 차이가 있는 것으로 나타났다. 5 MPa/18 hr의 강도 요구조건을 만족하기 위해서 OPC는 최소 $17^{\circ}C$ 이상, CHC는 $14^{\circ}C$ 이상, HESPC는 $11^{\circ}C$ 이상의 양생온도가 필요한 것으로 나타났다. 한편, 물-시멘트비에 따른 강도특성은 물-시멘트비가 낮을수록 우수한 강도발현을 보였다. 본 연구의 결과를 바탕으로 건설현장에서 활용된다면 조기강도를 앞당김으로써 공사기간 단축에 의한 공사원가 절감으로 경제적인 시공이 가능할 것으로 사료된다.

초기단계 기술의 가치평가 방법론 적용 프레임워크 (Framework for Technology Valuation of Early Stage Technologies)

  • 박현우;이종택
    • 기술혁신학회지
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    • 제15권2호
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    • pp.242-261
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    • 2012
  • 본 고에서는 연구기관에서 개발된 기술의 기술이전 가격결정을 위해 초기단계 기술의 가치평가를 포함하여 연구개발이 진행됨에 따른 단계별 가치평가 방법을 적용하기 위한 개념적 틀을 구상해보고자 한다. 전통적인 기술가치평가는 암묵적으로 기술이 제품에 적용되는 경쟁기술인 상태를 가정하는 경우가 많다. 그러나 초기단계 기술 또는 경쟁환경에 노출되기 이전 단계의 기술에 대한 가치는 단일의 평가방법만으로 평가하는 데 한계가 존재한다. 따라서 이와 같은 초기단계 기술에 대한 가치평가를 수행하기 위한 방법론 적용의 프레임워크를 제시하고자 하였다.

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한중 환경에서 정제된 급결제를 혼입한 플라이애시 콘크리트의 특성 (A Characteristics of Fly-ash Concrete Incorporating Tablet-shaped Accelerators in Cold Weather)

  • 이용수;류재석
    • 한국건설순환자원학회논문집
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    • 제2권1호
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    • pp.40-45
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    • 2014
  • 본 연구에서는 한중 콘크리트 타설시 발생되는 초기동해로 인한 피해를 방지하고 조강성을 향상시키기 위하여 기존 연구에서 이용된 정제(Tablet)화된 급결제를 사용 하였다. 특히 국내에서는 한중 콘크리트라고 해도 적은양의 플라이애시가 치환되고 있으므로 이러한 Tablet을 플라이애시 치환 콘크리트에 혼입하여 workability, early strength 및 동결융해 저항성을 비교 확인하였다. 그 결과 Tablet 0.5%, 1.0%가 가장 좋은 결과를 나타내어 일정시간 동안 작업시간 확보가 가능하며 초기동해 및 조기강도 특성을 증진 시킬 뿐만 아니라 높은 동결융해 저항성 및 내구성 지수를 가지고 있어 한중 콘크리트로의 적용이 가능하다는 것을 확인하였다.

Indicator-based Behavior Ontology for Detecting Insider Threats in Network Systems

  • Kauh, Janghyuk;Lim, Wongi;Kwon, Koohyung;Lee, Jong-Eon;Kim, Jung-Jae;Ryu, Minwoo;Cha, Si-Ho
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제11권10호
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    • pp.5062-5079
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    • 2017
  • Malicious insider threats have increased recently, and methods of the threats are diversifying every day. These insider threats are becoming a significant problem in corporations and governments today. From a technology standpoint, detecting potential insider threats is difficult in early stage because it is unpredictable. In order to prevent insider threats in early stage, it is necessary to collect all of insiders' data which flow in network systems, and then analyze whether the data are potential threat or not. However, analyzing all of data makes us spend too much time and cost. In addition, we need a large repository in order to collect and manage these data. To resolve this problem, we develop an indicator-based behavior ontology (IB2O) that allows us to understand and interpret insiders' data packets, and then to detect potential threats in early stage in network systems including social networks and company networks. To show feasibility of the behavior ontology, we developed a prototype platform called Insider Threat Detecting Extractor (ITDE) for detecting potential insider threats in early stage based on the behavior ontology. Finally, we showed how the behavior ontology would help detect potential inside threats in network system. We expect that the behavior ontology will be able to contribute to detecting malicious insider threats in early stage.