• YAKHAK HOEJI
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• v.57 no.6
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• pp.400-405
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• 2013

In this study, we measured the complex efficiency of a physiologically active antibody, a chelator and radiosiotopes using the ESI-TOF/Ms system for develop good radiopharmaceuticals. For a precise measurement, TLC is a low accuracy method. Loading of same amount of sample is difficult for each test, and work to quantify accurately the results obtained through TLC cannot be afforded compared to the use of other analytical instruments. The method of analysis using a mass spectrometer is capable of a mass analysis of proteins for quantitative analysis. The conjugates of the chelator (CHX-A- DTPA) and the antibody (IgG) were separated through MWCO, and were analyzed using ESI-TOF and MALDI-TOF mass spectrometry. The analysis using MALDI-TOF is roughly divided into measurements on mass spectrometry. When conjugating a small molecular weight of CHX-A-DTPA and a large molecular weight of IgG, distinguishing the peak of the conjugate and the peak of IgG was difficult. However, an ESI-TOF mass spectrometer system is capable of an analysis of mass by decentralizing the IgG. It is utilized as a technique for measuring the metabolic processes during conjugation and the stability evaluation of radiopharmaceuticals. When establishing this technique, the accuracy of the overall radiophar-maceutical analysis is expected to be able to be improved.

• Analytical Science and Technology
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• v.28 no.4
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• pp.299-307
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• 2015

In May 2012, a safety hazard issue arose because some kiwifruit growers in New Zealand had sprayed streptomycin to prevent kiwifruit canker. Therefore, for food safety management, analytical methods to determine streptomycin residues in kiwifruits are required. We developed an analytical method to determine streptomycin residues in kiwifruit samples using liquid chromatograph tandem mass spectrometer (LC-ESI-MS/MS). Streptomycin residues in samples were extracted using 1% formic acid in methanol, centrifugation for 10 min, and subsequent supernatant filtration. Purified samples were subjected to LC-ESI-MS/MS to confirm presence of and quantify streptomycin residues. Average streptomycin recoveries (6 replicates each sample) were in the range of 94.8%-110.6% with relative standard deviations of <10%. The linearity of the concentration range of 0.01-5.0 mg/kg using a matrix-matched calibration gave R² = 0.9995. The limit of quantification (LOQ) was 0.01 mg/kg. Results showed that our analytical method is rapid, simple, and sensitive, with easy sample preparation.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.405-409
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• 2018

최근 기후변화로 인해 기온, 강수량 등 농업에 직접적인 영향을 주는 환경요인의 변화가 급격하게 진행되고 있으며, 식량농업기구 (Food and Agriculture Organization of the United Nations, FAO)는 기후변화로 인해 전 세계적인 식량위기가 발생할 가능성이 크다고 경고하고 있다. 농업 시스템의 생산 능력을 확보하기 위해 수자원의 효율적인 공급 및 분배, 수확량 예측, 토지 특성 파악 등 농업 생산 제한요소에 대한 빠른 정보수집이 요구되고 있다. 재해관리 분야에서 원격탐사 기술은 재해 발생을 인지하고 발생지역의 재해 진행과 피해 정도를 신속하게 제공할 수 있다는 점에서 효용성이 높다. 또한 위성 영상을 이용할 경우 접근이 용이하지 못한 지역의 조사가 수월하며, 장기적인 변화관측이나 환경감시 등 광역적 접근이 가능하다. 최근 위성영상을 통한 다양한 신호의 데이터 취득 및 가공이 가능하게 됨에 따라 주기적이고 동일한 정확도로 지상자료의 획득이 가능하다는 측면에서 인공위성을 활용한 농업 분야에서의 가뭄 분석 연구의 필요성이 대두되었다. 위성영상 신호를 통해 농업 가뭄에 활용되고 있는 지표로는 정규식생지수 (Normalized Difference Vegetation Index, NDVI) 및 식생상태지수 (Vegetation Condition Index, VCI), 식생가뭄반응지수(Vegetation Drought Response Index, VegDRI) 등이 있다. 잠재 증발산과 실제 증발산의 비를 이용한 위성영상기반의 가뭄지수인 Evaporative Stress Index (ESI)는 일반적으로 사용되는 가뭄지수인 표준강수지수(Standardized Precipitation Index, SPI), 파머가뭄심도지수 (Palmer Drought Severity Index, PDSI) 등과 비교하였을 때, 가뭄에 더 민감하고 빠른 반응을 보인다는 연구 결과로부터 짧은 기간의 급속하게 발생하는(rapid-onset) Flash drought의 가뭄판단지표로 활용되고 있다. 본 연구에서는 과거 우리나라에 발생했던 극심한 가뭄 사상을 대상으로 ESI의 가뭄분석을 통해 타 지표와의 차별성을 확인하고 농업 가뭄 모니터링의 새로운 지표로써 적용성을 검토하고자 한다.

• International Area Studies Review
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• v.13 no.3
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• pp.583-611
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• 2009

This study aims at analysing the export competitiveness of the six largest countries which have the highest market share in world automobile market, namely Japan, USA, China, Germany, South Korea and France for the last seven years between 2000 and 2006 in Chinese automobile market by using revealed comparative advantage index(RCA), comparative advantage index by country (CAC) and exporting similarity index(ESI). On analysis of the RCA index, it showed that the export competitiveness of Korean automobile in the world market was high following Japan and Germany, but it ranked the lowest competitiveness among the six trade competitors in Chinese automobile market. In other side, it is also made sure that the formidable competitors of Korean automobile export in global market are Germany and Japan. Therefore, South Korea needs to raise its ability to adjust to improvement of the international competitiveness in exports through close cooperation between government and automobile export enterprise and consistent monitoring about Chinese automobile industry and market.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.3
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• pp.1-14
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• 2019

North Korea has frequently suffered from extreme agricultural crop droughts, which have led to food shortages, according to the Food and Agriculture Organization (FAO). The increasing frequency of extreme droughts, due to global warming and climate change, has increased the importance of enhancing the national capacity for drought management. Historically, a meteorological drought index based on data collected from weather stations has been widely used. But it has limitations in terms of the distribution of weather stations and the spatial pattern of drought impacts. Satellite-based data can be obtained with the same accuracy and at regular intervals, and is useful for long-term change analysis and environmental monitoring and wide area access in time and space. The Evaporative Stress Index (ESI), a satellite-based drought index using the ratio of potential and actual evaporation, is being used to detect drought response as a index of the droughts occurring rapidly over short periods of time. It is more accurate and provides faster analysis of drought conditions compared to the Standardized Precipitation Index (SPI), and the Palmer Drought Severity Index (PDSI). In this study, we analyze drought events during 2015-2017 in North Korea using the ESI satellite-based drought index to determine drought response by comparing with it with the SPI and SPEI drought indices.

• Korean Journal of Pharmacognosy
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• v.50 no.1
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• pp.65-71
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• 2019

One of the oriental medicine prescriptions, Sagunja-tang consists of four herbal medicines (Ginseng Radix, Poria Sclerotium, Atractylodis Rhizoma Alba, and Glycyrrhiziae Radix et Rhizoma) and has been used as a medicine to enhance tonify the function of spleen and stomach in Korea. In this study, we conducted simultaneous analysis of the 9 marker components, liquiritin apioside, liquiritin, ginsenoside Rg1, liquiritigenin, ginsenoside Rb1, glycyrrhizin, atractylenolide III, atractylenolide II, and atractylenolide I in Sagunja-tang using a liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). Marker compounds were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, 1.7 mm) and the column was maintained at $45^{\circ}C$. The mobile phase consists of 0.1% (v/v) aqueous formic acid and acetonitrile with gradient condition. The LC-MS analysis was performed using a Waters ACQUITY TQD LC-MS/MS system with multiple reaction monitoring (MRM) method in the positive and negative modes. The calibration curves of the nine marker components showed good linearity with coefficient of determination ${\geq}0.9984$ within tested range. The limits of detection and limits of quantification values were 0.27-2.42 ng/mL and 0.81-7.27 ng/mL, respectively. The concentrations of tested 9 analytes in the lyophilized Sagunja-tang sample using the established LC-ESI-MS/MS MRM method were detected up to 16.593 mg/g. These results can be useful as a basic data for the quality control of an oriental medicine prescriptions.

• CELLMED
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• v.11 no.1
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• pp.2.1-2.8
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• 2021

The clinical condition Amnesia causes difficulty in learning new information and the inability to recall past events. It is primarily concerned with recent memory loss. Majoon-e-Nisyan (MJN) is a polyherbal Unani formulation, present in a semi-solid form. It is widely used potent drug of the Unani System of Medicine (USM) for treating Nisyan (amnesia). In the present study polyherbal Unani formulation, MJN has been studied for its quality control and acute toxicity. Standardization (quality control) of drugs deals with drug identity, drug quality and purity determination. Standardization of MJN had been done as per the Unani pharmacopoeial parameters approved by World Health Organization (WHO) - Pharmacognostical parameters, Physico-chemical parameters, high-performance thin-layer chromatography (HPTLC), microbial load, aflatoxin, and heavy metals. Solvents and chemicals used in the study were of analytical grade and used instrument were calibrated. By conducting an acute oral toxicity study in rats, the safety of MJN was assessed. The limit test method of OECD guideline 425 was followed in the study. Results of standardization and standard operating procedures (SOPs) for preparation of MJN may serve as the standard reference in the future. The data generated in the study for the quality control of MJN proved the quality of formulation and shows that MJN is not toxic in rats following acute dosing up to 2000 mg/kg bw. The data obtained in the paper for MJN may be used as a standard guideline for preparation of the formulation which can save time, cost, and resources for future research endeavours.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.372-379
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• 2007

A high-performance liquid chromatography-electrospray ionization (HPLC-ESI) tandem MS was developed for the rapid and simultaneous determination of forbidden medicines in dietary supplements. Thirteen medicinal components such as PDE-5 inhibitors and their analogues, and the newly identified dimethylsildenafil and xanthoanthrafil, were included in this study. After tentative standardization of molecular ions in both polarities using thirteen references on the mass spectrometer, with ESI-continuous infusion via the syringe pump method, the relative intensity of the ions present in the resulting spectra was quantitatively compared. From the results, the ion mode was selected depending on each reference's characteristics. A HPLC method coupled with the ESI mode was developed considering the matrix effect and interference depending on the type of sample. The validation test of the developed method was followed by carrying out precision, accuracy, recovery, sensitivity and linearity, etc. The method showed sufficiently high sensitivity, reproducibility, and specificity, and produced 4 times faster results when compared with the existing HPLC/UV method for the determination of forbidden compounds in dietary supplements.

• Journal of Navigation and Port Research
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• v.35 no.9
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• pp.735-740
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• 2011

It is necessary to develop the one stop system in order to protect our marine environment rapidly from oil spill accident. The purpose of this study is to develop real time database for oil spill prediction modeling and implement real time prediction modelling with ESI and server-client GIS based user interface. The existing oil spill prediction model cannot provide one stop information system for public and government who should protect sea from oil spill accident. The development of multi user based information system permits integrated handling of real time meteorological data from external ftp. A server-client GIS based model is integrated on the basis of real time database and ESI map to provide the result of the oil spill prediction model. End users can access through the client interface and request analysis such as oil spill prediction and GIS functions on the network as their own purpose.

• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.1996-2000
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• 2009

Alkali metal salts were introduced to enhance the ionization efficiency of glucose and maltooligoses in electrospray ionization-mass spectrometry (ESI-MS). A mixture of the same moles of glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose was used. Salts of lithium, sodium, potassium, and cesium were employed as the cationizing agent. The ionization efficiency varied with the alkali metal cation types as well as the analyte sizes. Ion abundance distribution of the [M+$cation]^+$ ions of the carbohydrates varied with the fragmentor voltage. The maximum ion abundance at low fragmentor voltage was observed at maltose, while the maximum ion abundance at high fragmentor voltage shifted to maltotriose or maltotetraose for Na, K, and Cs. Variation of the ionization efficiency was explained with the hydrated cation size and the binding energy of the analyte and alkali metal cation.