• 약학회지
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• 제60권1호
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• pp.15-20
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• 2016

Globotriaosylsphingosine (lyso Gb3) is considered as one of the biomarkers for Fabry disease. A rapid and simple UPLC-MS/MS method was developed for the determination of reliable biomarker, lyso Gb3. Total analytical procedure takes only 15 min including sample preparation and MS/MS analysis. Limit of detection was 0.85 ng/ml (S/N=3). The calibration curve was linear over the range of 2.0~400.0 ng/ml ($R^2=0.9999$). Inter-day and intra-day assay accuracy were 93.4~100.6% (RSD, 0.6~6.0%) and 97.5~100.7% (RSD, 3.6~5.2%). Absolute recoveries of 97.6~98.6 showed excellence of a new analytical method. The method was applied to human and mice urines, proved the suitability for the quantification of lyso-Gb3 for screening, diagnosis and therapeutic monitoring of Fabry disease patients.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.442-447
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• 2002

When a SAR system operates in a full-polarimetic mode, the amount of the information one can extract is so complex that the effective presentation of the information is important. However, the information acquired from the polarimetric SAR data is often difficult to interpret by itself, because it is consisted of both the amplitude information and the phase information. Polarimetric parameters are the good way of representing the polarimetric SAR information in a quantitative manner. Also they can characterize the scattering behavior of the ground scatterer. In this research, extraction of polarimetric parameters, evaluation and interpretation of the scattering behavior of the ground with respect to polarimetric SAR signal are carried out. Using the NASA/JPL AIRSAR data, we estimated the polarimetric parameters and compared them in terms of the ground features. In general, extracted parameters well represent the characteristics of the different features on the ground.

• Natural Product Sciences
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• 제4권4호
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• pp.245-252
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• 1998

The unique sulphated phenolics, gallic acid 3-methyl ether 5-potassium sulphate, isoferulic acid 3-potassium sulphate, and ellagic acid 4,4'-dimethyl ether 3-potassium sulphate have been isolated from the flowers of Tamarix amplexicaulis Ehrenb. (Tamaricaceae). The hitherto unknown natural phenolic acid, gallic acid 3-methyl ether, together with the known phenolic, gallic acid, gallic acid 4-methyl ether, isoferulic acid, ferulic acid, ellagic acid, and ellagic acid 4,4'-dimethyl ether have been also separated and characterized. The structures were established by conventional methods, including electrophoretic analysis and confirmed by ESI-MS, $^1H-\;and\;^{13}C-NMR$.

• Mass Spectrometry Letters
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• 제9권1호
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• pp.17-23
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• 2018

Studies on the interactions of amyloidogenic proteins with trace metals, such as copper, have indicated that the metal ions perform a critical function in the early oligomerization process. Herein, we investigate the effects of Cu(II) ions on the active sequence regions of amyloidogenic proteins using electrospray ionization mass spectrometry (ESI-MS) and collision induced dissociation tandem MS (CID-MS/MS). We chose three amyloidogenic peptides NNQQNY, LYQLEN, and VQIVYK from yeast prion like protein Sup35, insulin chain A, and tau protein, respectively. [Cu-peptide] complexes for all three peptides were observed in the mass spectra. The mass spectra also show that increasing Cu(II) concentrations decrease the population of existing peptide oligomers. The tandem mass spectrum of NNQQNY shows preferential binding for the N-terminal region. All three peptides are likely to appear to be in a Cu-monomer-monomer (Cu-M-M) structure instead of a monomer-Cu-monomer (M-Cu-M) structure.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.227-228
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• 2009

• Journal of Industrial and Engineering Chemistry
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• 제68권
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• pp.282-292
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• 2018

This study aimed to compare the chemical composition of Morus nigra leaves extracts, obtained by maceration, accelerated solvent (ASE) and supercritical fluid extraction (SFE) under different extraction conditions. With regards to chemical composition, mainly phenolic acids and flavonoids were identified. HPLC-ESI-QTOF-MS allowed the identification of 13 new compounds reported in M. nigra leaves for the first time. ASE as a fast, green and innovative approach, seems to be the best choice for extracting compounds of different polarities within the shortest extraction time. The present study also highlights the potential application of M. nigra extracts as constituents of new added-value formulations.

• Mass Spectrometry Letters
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• 제12권4호
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• pp.137-145
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• 2021

Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

• Annals of Fuzzy Mathematics and Informatics
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• 제16권3호
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• pp.337-361
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• 2018

We introduce and study some basic properties of rough $I_{\lambda}$-statistical convergent of weight g (A), where $g:{\mathbb{N}}^3{\rightarrow}[0,\;{\infty})$ is a function statisying $g(m,\;n,\;k){\rightarrow}{\infty}$ and $g(m,\;n,\;k){\not{\rightarrow}}0$ as $m,\;n,\;k{\rightarrow}{\infty}$ and A represent the RH-regular matrix and also prove the Korovkin approximation theorem by using the notion of weighted A-statistical convergence of weight g (A) limits of a triple sequence of Bernstein-Stancu polynomials.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.217.3-217.3
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• 2003

Acetyl-L-carnitine, a physiological component of the L-carnitine family, has been proposed for treating Alzheimer's disease in pharmacological doses. Acetyl-L-carnitine and d3-acetylcarnitine (internal standard) were analyzed by electrospray ionization / tandem mass spectrometry (ESI/MS/MS) after derivatization to their butylesters through treatment with butanolic hydrogen chloride. Acetyl-L-carnitine produced a protonated precursor ion at m/z 260 and a corresponding product ion of m/z 85. Analytes were separated on a Capcell Pak C18 (2.0${\times}$150mm, 5 mm). (omitted)

• Natural Product Sciences
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• 제26권4호
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• pp.334-339
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• 2020

A new compound, 3��-acetoxylanosta-7,9(11),24-triene-26-al (3), and seven known compounds (1 - 2 and 4 - 8) were isolated from Ganoderma tropicum (Jung.) Bres. collected in Tay Nguyen, Vietnam. The structures of these compounds were determined by one- and two-dimensional nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and high-resolution ESI-MS, and by comparison with literature data. All of the isolated compounds were tested for nitroblue tetrazolium (NBT) reduction activity in Saccharomyces cerevisiae-stimulated RAW 246.7 cells. Among them, compounds 2 - 4 and 6 - 8 enhanced the NBT reduction in a dose-dependent manner.