• Applied Biological Chemistry
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• v.33 no.1
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• pp.87-92
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• 1990

To produce ergosterol Saccharomyces toke KBA No. 6 was used and various factors related to ergosterol accumulation were investigated. The most effective inorganic nitrogen source was ammonium chloride and 3.50 % of ergosterol was accumulated in the cell when the C/N ratio was 200/1. When Tween 80 and potassium nitrite were used simultaneously, ergosterol content and total amount of ergosterol were increased by 56 % and 45 %, respectively, compared to their control in which 0.2 % of Tween 80 and 0.1 % of potassium nitrite were used. Under the optimum conditions, ergosterol content increased from 1.73 % to 5.3 % and the total amount of ergosterol was increased from 65.2 mg/l to 135.15 mg/l.

• Journal of the Korean Wood Science and Technology
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• v.28 no.1
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• pp.65-70
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• 2000

Ergosterol is an indicator chemical of fungi and involves in fungal cell growth as a major component in fungal cell membranes. Thus, this chemical can be used to estimate live fungal biomass within various solid substrates. Ergosterol content in liquid culture of Lentinula edodes had a linear relationship (r=0.98) with the hyphal mass of the fungus. This chemical content differed depending on the fungal strains, age of culture and water content levels of sawdust substrate. Ergosterol content was 0.13% in the 10 weeks old liquid culture while it was 0.10% in 20 weeks old one. The chemical content in the sawdust cultures of the fungus varied 0.015% to 0.042% depending upon strains and water content levels within sawdust substrate. Ergosterol content in the culture of a L. edodes strain, Sanrim 4, was higher by $20{\mu}g$ to $140{\mu}g$/g dry substrate than those of strains, Mok-H and Sanrim 6. And the chemical contents in the sawdust cultures with 125% or 175% water, 297 to 425 ${\mu}g$/g dry substrate, were higher than those with 75% or 225% water, 148 to $286{\mu}g$/g dry substrate. We conclude that ergosterol analysis can estimate the fungal biomass within solid substrate such as logs and sawdust.

• Mycobiology
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• v.37 no.1
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• pp.10-16
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• 2009

The purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in Tricholoma matsutake soil colonies during the mushroom fruiting season. The active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular T. matsutake colony at Ssanggok valley in the Sogri Mt. National Park in Korea during 2003 to 2005. The four points were the ahead of the colony, the front edge of the colony and 20 cm and 40 cm back from the front edge of the colony. Ergosterol content was 0.0 to 0.7 ${\mu}g$ per gram dried soil at the ahead, 2.5 to 4.8 ${\mu}g$ at the front edge, 0.5 to 1.8 ${\mu}g$ at the 20 cm back and 0.3 to 0.8 ${\mu}g$ at the 40 cm back. The ergosterol content was very high at the front edge where the T. matsutake hyphae were most active. However, ergosterol content did not significantly change during the fruiting season, September to October. Soil water contents were lower at the front edge and 20 cm back from the front edge of the colony than at the ahead and 40 cm back during the fruiting season. Soil water content ranged from 12 to 19% at the ahead, 10 to 11% at the edge, 9 to 11% at the 20 cm back and 11 to 15% at the 40 cm back. Our results suggest that the active front edge of the T. matsutake soil colony could be managed in terms of water relation and T. matsutake ectomycorrhizal root development.

• Archives of Pharmacal Research
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• v.20 no.3
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• pp.201-205
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• 1997

A very high content (at least 0.23%) of ergosterol peroxide was isolated from Naematoloma fasciculare Karst. Not only ergosterol peroxide but also ergosterol showed very strong anticomplementary activity on the classical pathway, the $IC_{50}$ values being $5.0 {\mu}M$ and $1.0 {\mu}M$, respectively. The $ ^{1}H $and $^{13}C$ NMR data of ergosterol peroxide were revised and completely assigned by DEPT, $^{1}H-^{1}H$ COSY, HMQC and HMBC correlations.

• Journal of Microbiology and Biotechnology
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• v.30 no.12
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• pp.1835-1842
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• 2020

Ergosterol, an essential constituent of membrane lipids of yeast, is distributed in both the cell membrane and intracellular endomembrane components such as vacuoles. Honokiol, a major polyphenol isolated from Magnolia officinalis, has been shown to inhibit the growth of Candida albicans. Here, we assessed the effect of honokiol on ergosterol biosynthesis and vacuole function in C. albicans. Honokiol could decrease the ergosterol content and upregulate the expression of genes related with the ergosterol biosynthesis pathway. The exogenous supply of ergosterol attenuated the toxicity of honokiol against C. albicans. Honokiol treatment could induce cytosolic acidification by blocking the activity of the plasma membrane Pma1p H+-ATPase. Furthermore, honokiol caused abnormalities in vacuole morphology and function. Concomitant ergosterol feeding to some extent restored the vacuolar morphology and the function of acidification in cells treated by honokiol. Honokiol also disrupted the intracellular calcium homeostasis. Amiodarone attenuated the antifungal effects of honokiol against C. albicans, probably due to the activation of the calcineurin signaling pathway which is involved in honokiol tolerance. In conclusion, this study demonstrated that honokiol could inhibit ergosterol biosynthesis and decrease Pma 1p H+-ATPase activity, which resulted in the abnormal pH in vacuole and cytosol.

• Mycobiology
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• v.35 no.4
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• pp.205-209
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• 2007

Ergosterol involves in fungal cell growth as a major component in fungal cell membranes. It can be an indicator that shows the fungal activity, and its content depends on the fungal strains, culture, growth conditions and so on. In this study, fungal activities and growth patterns of three white-rot fungi strains isolated in Korea were evaluated by determination of ergosterol contents during the incubation. Wood decay test and chemical analyses of wood were also performed to verify the relationship between fungal activity and wood degrading capacity of white-rot fungi for 60 days. In the results of experiments, it is considered that the test strains selectively degrade large amount of lignin in wood at the early stage of decay. Especially, Phanerochaete chrysosporium showed the best capability on selective degradation of lignin among the test fungi. It is suggested that the determination of ergosterol content in the fungal culture during the incubation is the simple and effective screening method of white-rot fungi for the application to biopulping of wood.

• Journal of Environmental Science International
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• v.29 no.12
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• pp.1199-1204
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• 2020

Lentinula edodes mushrooms cultivated under different relative humidities were wrapped at 4℃ and the results of storage characteristics were investigated. Changes in water content of fruiting bodies during the storage period showed the highest water content in fruit bodies harvested from the treatment with the highest relative humidity. The luminosity of the fresh fruiting bodies showed no significant change during the storage period, and the redness was higher in the relative humidity 95% treatment than in the other treatments. According to this study, the relative humidity of the pileus was 65%, and the content of Ergosterol was 0.67 ± 15 g / L at relative humidity of 65%, 80% and 95%. In addition, amino acid analysis and Principal Component Analysis (PCA) confirmed that methionine was the main cause of changes in storage time and relative humidity.

• Applied Biological Chemistry
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• v.32 no.3
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• pp.270-277
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• 1989

In order to elucidate the action mechanism of prochloraz and triadimefon, the mycelia of the Valsa ceratosperma were treated with the compounds in vitro. Analysis by GLC of the sterol extract from Valsa ceratosperma mycelia revealed one major peak and two minor peaks. Their relative retention time(RRT) relative to chloresterol were 1.29, 1.48 and 1.82, The compounds with RRT 1.29 and RRT 1.82 were identified as ergosterol and 24-methylenedihydrolanosterol by GC/MS, respectively. Five ppm of prochloraz and triadimefon applied to mycelia caused decrease in ergosterol content, whereas increase in 24-methylenedihydrolanoserol content in mycelia. The longer treatment time and the higher concentrations of the chemicals resulted in the greater decrease in ergosterol and the greater increase in 24-methylenedihydrolanosterol. Based on the analysis, it is considered that the two chemicals inhibit the ergosterol biosynthesis in Valsa ceratosperma by blocking C-14 demethylation as found previously in other fungi arid yeasts.

• Natural Product Sciences
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• v.19 no.3
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• pp.258-262
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• 2013

Three ergosterol derivatives, ergosta-4,6,8(14),22-tetraen-3-one (1), ergosta-7,24(28)-dien-3-ol (2), and 5,8-epidioxyergosta-6,22-dien-3-ol(3) were isolated from the fruit body of Phellinus pini. Their structures were based on spectroscopic methods including IR, MS, and NMR (1D and 2D). These compounds were evaluated for their activity to decrease melanin production in ${\alpha}$-MSH (melanocyte stimulating hormone) activated B16F10 cells. Compound 1, 2, and 3 reduced melanin content in a dose-dependent manner at concentrations of 5~15 uM. They also suppressed the tyrosinase expression of protein and m-RNA level dose dependently by western blot analysis and RT-PCR experiment in B16F10 murine melanoma cells.

• The Korean Journal of Food And Nutrition
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• v.36 no.2
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• pp.129-136
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• 2023

In order to investigate the optimal UVB (ultraviolet B) treatment conditions for vitamin D₂ enhancement of freeze-dried ear mushroom, sample size (below 300 ㎛~whole), UV treatment temperature (30~60℃), treatment density (6.25~50.0 mg/cm²) and the samples mixing frequency (1~32 times) were treated differently. After that, chromaticity, vitamin D₂ and ergosterol (vitamin D₂ precursor) contents were investigated. As a result of the investigating, effective UVB treatment conditions for vitamin D₂ enhancement are as follows. The sample sizes were 2~4 mm and finely crushed pieces. The treatment temperatures were 50℃ and 60℃. The treatment density was 12.5 to 25.0 mg/cm², and the number of sample mixing was 8 times or more. As the amount of vitamin D₂ increased by UVB treatment, the ergosterol content generally tended to decrease. However, under some UVB treatment conditions, the vitamin D₂ content was not high despite the decrease of ergosterol content. Under the conditions set in this experiment, it was possible to obtain ear mushrooms with enhanced vitamin D₂ up to 26,968.7 ㎍/100 g. Therefore, it is thought that the ear mushroom is highly likely to be used as a vitamin D source and nutritionally fortified food ingredient.