• Journal of Microbiology and Biotechnology
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• v.8 no.6
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• pp.553-559
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• 1998

cDNA of human thrombopoietin (hTPO) amplified by polymerase chain reaction from a cDNA library of human fetal liver was cloned. EPO-like domains ($hTPO_{153} \;or\; hTPO_{l63})\; of\; hTPO(hTPO_{332}$) were expressed in Escherichin coli using several kinds of expression systems, such as ompA secretion, thioredoxin fusion, and the $P_L$ and T7 expression systems. To obtain $hTPO_{153}$ in soluble form, $hTPO_{153}$ cDNA was fused in-frame behind the gene encoding ompA signal sequence and thioredoxin protein. When fused with either of the genes, $hTPO_{153}$ was not expressed to the detectable level. However, a high level expression of the EPO-like domain of hTPO was obtained using the PL and T7 expression system. $hTPO_{153} \;or\; hTPO_{l63} cDNA were subcloned into the pLex and pET-28a(＋) vectors under the control of the inducible$ P_L\;T_7$ promoter, respectively. Proteins expressed using pl.ex vector and pET-28a(＋) detected in insoluble forms with an expression level of about 14% and 9% of total cellular proteins, respectively, and the level of expression was rapidly diminished in 2 h after the maximum level of expression was reached.

• The Journal of the Korea Contents Association
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• v.8 no.4
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• pp.264-272
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• 2008

In ubiquitous geographic information environment, the users are provided with geographic information anywhere and any time on their needs by any types of devices and communication media. The unique location ID, ePosition, is a new technology to support UBGI environment, using a logical location ID instead of physical location of a point of interest. Domain names of plural ePosition servers, where location information with its ePosition is stored, needs to be registered in DNS for some service through Internet. For better ePosition service of the type of public service like email service, a set of DNS resource records can be newly defined. This paper suggests a new DNS resource record EPO for implementation of the ePosition service through Internet.