• Title/Summary/Keyword: EPI

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Development of a Total Internal Reflection Fluorescence (TIRF) Microscopy for Precise Imaging the Drying Pattern of a Sessile Droplet (고착 액적 증발면의 정밀 관측을 위한 전반사 형광 현미경 기법 개발)

  • Wonho Cho;Jinkee Lee
    • Journal of the Korean Society of Visualization
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    • v.21 no.3
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    • pp.65-74
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    • 2023

  • Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

  • https://doi.org/10.5407/jksv.2023.21.3.065 인용 PDF

Half Fourier T2 weighted EPI

  • 안창범;김치영;김휴정;문길영;김남주;정성택;이흥규;신경섭
    • Proceedings of the KSMRM Conference
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    • 2000.11a
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    • pp.19-19
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    • 2000

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