• 한국응급구조학회지
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• 제25권2호
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• pp.39-53
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• 2021

Purpose: Emergency medical technicians (EMTs) have so far been trained as professionals under the same conditions, with no change in the 1995 Act. We aimed to find ways for them to secure expertise in accordance with social needs by strengthening the quality of the EMT education. Methods: This is a descriptive study comparing the operation status of the national emergency medical examination conducted by the Korea Health Personnel Licensing Examination Institute, and the national examinations of EMT paramedic and EMT basic. The scope of the national examinations for EMT was compared by subject and area. Results: The national written exam for EMT paramedic consists of five areas. EMT basic does not include basic medicine in three related subjects, 11 areas, and 18 detail areas. Paramedic care does not include advanced pediatric life support. In addition, nine areas and 20 detail areas are not included. Conclusion: The study suggests the need for institutional supplementation so that those who have completed EMT basic and the subjects prescribed by the ordinance of the Ministry of Health and Welfare at universities, etc., in the Higher Education Act can take the EMT paramedic national exam.

• 한국응급구조학회지
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• 제14권2호
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• pp.57-69
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• 2010

Purpose : The National Registry Exam had some problems on validity of EMT evaluation and verification. The aim of this study was to propose a New EMT National Registry Exam. Methods : Conducted using the Delphi Method(lst meeting March 29 in 2008, 2nd meeting May 31 in 2008, 3rd meeting September 20 in 2008, 4th meeting January 16 in 2009) from questionnaire data(391 from Paramedic, 317 from Basic EMT) collected between June 16-30(lst questionnaire) and October 16-30(2nd questionnaire) in 2008 and a public hearing on February 5 in 2009. Results: The subjects proposed at the request of the National Health Personnel Licensing Examination Board were Introduction, Medical+Trauma Emergencies(Paramedic & Basic) for written examination and scenarios+protocols(Paramedic), protocols(Basic) for practical examination. Conclusion : To be able to response patients' problems in various emergencies, this integrated examination was selected and proposed.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4271-4274
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• 2014

The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.

• International Journal of Stem Cells
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• 제16권1호
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• pp.52-65
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• 2023

Background and Objectives: Epithelial-Mesenchymal transition (EMT) is one of the origins of myofibroblasts in renal interstitial fibrosis. Mesenchymal stem cells (MSCs) alleviating EMT has been proved, but the concrete mechanism is unclear. To explore the mechanism, serum-free MSCs conditioned medium (SF-MSCs-CM) was used to treat rat renal tubular epithelial cells (NRK-52E) fibrosis induced by transforming growth factor-β1 (TGF-β1) which ameliorated EMT. Methods and Results: Galectin-3 knockdown (Gal-3 KD) and overexpression (Gal-3 OE) lentiviral vectors were established and transfected into NRK-52E. NRK-52E fibrosis model was induced by TGF-β1 and treated with the SF-MSCs-CM for 24 h after modelling. Fibrosis and autophagy related indexes were detected by western blot and immunocytochemistry. In model group, the expressions of α-smooth muscle actin (α-SMA), fibronectin (FN), Galectin-3, Snail, Kim-1, and the ratios of P-Akt/Akt, P-GSK3β/GSK3β, P-PI3K/PI3K, P-mTOR/mTOR, TIMP1/MMP9, and LC3B-II/I were obviously increased, and E-Cadherin (E-cad) and P62 decreased significantly compared with control group. SF-MSCs-CM showed an opposite trend after treatment compared with model group. Whether in Gal-3 KD or Gal-3 OE NRK-52E cells, SF-MSCs-CM also showed similar trends. However, the effects of anti-fibrosis and enhanced autophagy in Gal-3 KD cells were more obvious than those in Gal-3 OE cells. Conclusions: SF-MSCs-CM probably alleviated the EMT via inhibiting Galectin-3/Akt/GSK3β/Snail pathway. Meanwhile, Gal-3 KD possibly enhanced autophagy via inhibiting Galectin-3/Akt/mTOR pathway, which synergistically ameliorated renal fibrosis. Targeting galectin-3 may be a potential target for the treatment of renal fibrosis.

• BMB Reports
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• 제55권8호
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• pp.370-379
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• 2022

Human pregnancy is a delicate and complex process where multiorgan interactions between two independent systems, the mother, and her fetus, maintain pregnancy. Intercellular interactions that can define homeostasis at the various cellular level between the two systems allow uninterrupted fetal growth and development until delivery. Interactions are needed for tissue remodeling during pregnancy at both fetal and maternal tissue layers. One of the mechanisms that help tissue remodeling is via cellular transitions where epithelial cells undergo a cyclic transition from epithelial to mesenchymal (EMT) and back from mesenchymal to epithelial (MET). Two major pregnancy-associated tissue systems that use EMT, and MET are the fetal membrane (amniochorion) amnion epithelial layer and cervical epithelial cells and will be reviewed here. EMT is often associated with localized inflammation, and it is a well-balanced process to facilitate tissue remodeling. Cyclic transition processes are important because a terminal state or the static state of EMT can cause accumulation of proinflammatory mesenchymal cells in the matrix regions of these tissues and increase localized inflammation that can cause tissue damage. Interactions that determine homeostasis are often controlled by both endocrine and paracrine mediators. Pregnancy maintenance hormone progesterone and its receptors are critical for maintaining the balance between EMT and MET. Increased intrauterine oxidative stress at term can force a static (terminal) EMT and increase inflammation that are physiologic processes that destabilize homeostasis that maintain pregnancy to promote labor and delivery of the fetus. However, conditions that can produce an untimely increase in EMT and inflammation can be pathologic. These tissue damages are often associated with adverse pregnancy complications such as preterm prelabor rupture of the membranes (pPROM) and spontaneous preterm birth (PTB). Therefore, an understanding of the biomolecular processes that maintain cyclic EMT-MET is critical to reducing the risk of pPROM and PTB. Extracellular vesicles (exosomes of 40-160 nm) that can carry various cargo are involved in cellular transitions as paracrine mediators. Exosomes can carry a variety of biomolecules as cargo. Studies specifically using exosomes from cells undergone EMT can carry a pro-inflammatory cargo and in a paracrine fashion can modify the neighboring tissue environment to cause enhancement of uterine inflammation.

• 한국응급구조학회지
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• 제8권1호
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• pp.19-32
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• 2004

This study was conducted to identify an initial fire station experience of EMT students, so to better understand their' practice experience in fire station. The subjects were 28 EMT students of C department of Emergency Medical technology in C city, who were demonstrating at the fire station in C city. This study was approached by phenomenological method, collected data were analyzed by Colaizzi's method, the results were as a follows. From the protocol, 201 significant statements were organized into 93 formulated meanings. From the formulated meanings, 30 themes were identified, organized into 16 theme clusters, and then 7 categories. EMT students got experienced 'tension' in resulting from new training situation and at the field practice, 'comport and gratitude', in feeling of identity and a bond sympathy with senior EMT, in training environment and heartfelt care, 'stress' from lack of knowledge and skill, difficulties in field practice for 24 hours, in dealing with making interpersonal relationship with patient and staffs and from the insufficiency of instruction, 'confidence feeling' from the participation of field treatment, in improving of learning, in self-esteem of EMT job, 'confusing feeling' of conflict of the path in work, 'disappointment and doubt' by the discrepancy between learning and actuality, and disappointment of actuality, 'feeling of lack' based on the passive attitude. The results of this study are to use as basic data for students attending fire station experience for the first time.

• 한국응급구조학회지
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• 제11권2호
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• pp.5-17
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• 2007

Purpose: The purpose of this study was to supply basic data for a health promoting program and to elevate the level of it by examining whether EMT-P Students' health promoting behaviors were related to health percetion, health concept, health status, self-esteem, perceived benefits of action, perceived barriers of action, perceived self-efficacy, activity-related affect, social support, preference, prior related behavior, and a plan for action. Method: Subjects were 116 EMT-P Students in K city. Data collection method was a structured questionnaire. Data was analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation analysis, and stepwise multiple regression. Result: The most powerful predictor was prjor related behavior(28.8%). Altogether prjor related behavior, health status, perceived barriers of action, a plan for action were proven to account for 44.6% of health promoting behaviors of EMT-P Students. Conclusion: It suggested that prjor related behavior, health status, perceived barriers of action, a plan for action should be considered when developing a EMT-P Students' health promoting program.

• 한국응급구조학회지
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• 제7권1호
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• pp.95-108
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• 2003

This study was conducted to identify an initial clinical experience of EMT students, so to better understand their' experience in clinical training. The subjects were 30 EMT students of C department of Emergency Medical technology in C city, who were demonstrating at the emergency room in C, T, S, W city. This study was approached by phenomenological method, collected data were analyzed by Colaizzi's method. the results were as a follows. From the protocol, significant statements were organized into 34 formulated meanings. from the formulated meanings, 21themes were identified, organized into 8 theme clusters, and then categories. EMT students got experienced 'tension resulting from new situation', 'fear in contacting with patients', 'lack of knowledge and skill', 'confidence feeling from being adapted', 'facing up to Paramedic role', 'experience of death' and 'stress'. The results of this study are to use as basic data for students attending clinical experience for the first time.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7617-7623
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• 2014

Objective: The mechanism of action of fatty acid synthase (FASN) in drug tolerance of breast cancer cells with epithelial-mesenchymal transition (EMT) features was investigated. Methods: The breast cancer cell line MCF-7-MEK5 with stably occurring EMT and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) tolerance was used as the experimental model, whereas MCF-7 acted as the control. Tumour cells were implanted into nude mice for in vivo analysis, and cerulenin was used as a FASN inhibitor. RT-PCR, real-time quantitative PCR and Western blot were employed to detect the expression of FASN, TNFR-1, TNFR-2, Wnt-1, ${\beta}$-catenin and cytC at the RNA and protein levels. Results: Compared with MCF-7, TNFR-1 expression in MCF-7-MEK5 was slightly changed, TNFR-2 was decreased, and FASN, Wnt-1, ${\beta}$-catenin and cytC were increased. The expression of Wnt-1 and ${\beta}$-catenin in MCF-7-MEK5 decreased after cerulenin treatment, whereas cytC expression increased. Conclusions: The important function of FASN in the drug tolerance of breast cancer may be due to the following mechanisms: FASN downregulated TNFR-2 expression through lipid rafts to make the cells less sensitive to TNF-${\alpha}$, and simultaneously activated the Wnt-$1/{\beta}$-catenin signalling pathway. Thus, cytC expression increased, which provided cells with anti-apoptotic capacity and induced drug tolerance.

• 한국산학기술학회논문지
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• 제14권6호
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• pp.2908-2914
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• 2013

본 연구는 대전,충남 소재 대학병원에 근무하는 응급의료종사자 122명을 대상으로 병원 내 응급구조사를 조사하여 응급구조사의 역할과 법적 업무규정의 현실적 제고 및 병원 내 응급구조사의 업무에 관한 인식을 보다 명확히 하기 위한 기초자료를 제공 하고자 연구를 실시하였다. 연구 대상자들의 병원 내 응급구조사의 업무인식을 살펴본 결과, 전체 40문항에 대해 $3.23{\pm}0.67$점으로 나타나 병원 내 응급구조사에 대한 업무인식정도는 중간 보다 다소 높은 것으로 나타났다. 영역별로는 외상처치가 가장 높게 나타났으며, 약물투여가 가장 낮았다. 응급의료종사자가 응급구조사의 업무를 바르게 인식하게 하기 위해서는 응급구조사가 고유 업무와 역할을 충실히 해 낼 수 있어야 할 것이다. 그러므로 보다 명확한 교육과정이 이루어져야 하며 보다 명확한 업무가 법적으로 규정되어져야 한다. 응급구조사에 대한 홍보와 지속적인 모니터링이 필요할 것으로 사료된다.