• Title/Summary/Keyword: EM-S

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Influence of Supplemental Enzymes, Yeast Culture and Effective Micro-organism Culture on Gut Micro-flora and Nutrient Digestion at Different Parts of the Rabbit Digestive Tract

  • Samarasinghe, K.;Shanmuganathan, T.;Silva, K.F.S.T.;Wenk, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.830-835
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    • 2004
  • An experiment of 10 weeks duration was carried out to study the influence of supplemental effective microorganism (EM) culture, yeast culture and enzymes on nutrient digestibility and gut microflora in rabbit gastrointestinal (GI) tract. Twenty four eight to nine weeks old, New Zealand White rabbits were allotted to four dietary treatments; a basal (control) feed, basal feed supplemented with either EM (1%), yeast culture or enzymes (400 ppm). Nutrient flow in digesta and their digestibility at ileum, caecum, colon and in the total tract as well as gut microflora distribution were studied. Feed dry matter was diluted from 92% to about 14% up to the ileum and about 95% of this water was reabsorbed by the colonic rectal segment followed by caecum (25%). EM and yeast improved protein digestibility at a lower rate than enzymes. Ileal, caecal, colonic and total tract digestibility of crude protein with enzymes were higher by 10.8, 9.4, 11.3 and 10.7%, respectively, as compared to the control. Yeast and enzymes increased crude fiber digestibility at ileum, caecum, colon and in the total tract by 8.5, 9.6, 9.0 and 8.3%, respectively, while EM improved them at a lower rate. Irrespective of treatments, total tract digestibility of crude protein (0.698-0.773) and fiber (0.169-0.183) were greater (p<0.05) than the ileal digestibility. Even though a post-caecal protein digestibility was observed, fiber digestion seemed to be completed in the caecum especially with yeast and enzymes. High precaecal digestibility of crude fiber (97%) and protein (95%) were observed even without additives probably due to caecotrophy. EM and yeast culture promoted the growth of lactic acid bacteria especially in the caecum but they did not influence gut yeast and mould. Present findings reveal that even though rabbits digest nutrients efficiently through hind gut fermentation, they can be further enhanced by EM, yeast and enzymes. Of the three additives tested, enzymes found to be the best.

Applicability of the Small-Loop EM Method in the Sallow Marine Environment (천해 환경에서 소형루프 전자탐사의 적용성)

  • Song, Sung-Ho;Kim, Rae-Young;Kang, Hye-Jin;Cho, In-Ky
    • Geophysics and Geophysical Exploration
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    • v.14 no.2
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    • pp.152-157
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    • 2011
  • The small-loop electromagnetic (EM) method is one of the rapid and non-destructive geophysical methods and has been used widely for many geophysical investigations, particularly for shallow engineering and environmental surveys. Especially in the shallow marine environment, the small-loop EM technique is very effective because of rapid and convenient data acquisition, large signal and low noise level. However, the method has been rarely applied in the very conductive marine environment since it's penetration or investigation depth might be considered too low. In this study, we demonstrated that the small-loop EM method can be effectively applied in the extremely conductive marine environment through the analysis of 1D small-loop EM data. Furthermore, we confirmed that the resistivity distribution under the sea bottom can be quantitatively predicted from the 1D inversion results of synthetic and field data.

Development of the EM Wave Absorber for the Collision-Avoidance Radar of ITS (ITS의 차량충돌방지 레이더용 전파흡수체 개발)

  • Kim Dong-Il;Choi Chang-Mook;Ko Kwang-Soob
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.43 no.9 s.351
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    • pp.1-5
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    • 2006
  • In this paper, the EM wave absorber was designed and fabricated for the collision-avoidance radar as a basic sensor of ITS(Intelligence Transport System), because radar system has some problems including false image and system-to-system interference. We fabricated some samples in different composition ratio of Carbon and CPE, and defined that optimum composition ratio of Carbon and CPE was 20:80 wt%. The complex relative permittivity was calculated by the measured data. And absorption abilities were simulated according to different thickness of the EM wave absorbers using the complex relative permittivity. The EM wave absorber was manufactured based on the simulated design. Simulated and measured results agree very well. As a result, the developed EM wave absorber with the thickness of 2 mm has absorption ability over 20 dB in frequency range of $76{\sim}77$ GHz.

A Comparison of the Textbooks for Elementary Mathematics Between Korea and U.S.A about Congruence of Figures (우리나라 초등 수학 교과서와 미국 EM 교과서 비교 - 도형의 합동을 중심으로 -)

  • Son, Min-Gyeong;Ryu, Heuisu
    • Journal of Elementary Mathematics Education in Korea
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    • v.18 no.3
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    • pp.539-555
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    • 2014
  • In this study, an implication has been drawn for the textbook development and teaching and learning process as a congruence of a figure is compared and analyzed between Korean elementary mathematics textbooks and American elementary math textbooks. Based on the result of comparison and analysis in congruence contents between Korean and EM textbooks, some applications for the development of figure and congruence chapters in Korean textbooks are as below. First of all, in term of congruence, activities related to congruence need to be introduced after the concept of congruence is defined either with illustrations of fundamental figures such as a segment and angle or with examples of polygon. Second, it is required to assist students to realize that compasses can be used to copy length. In Korean textbooks, compasses are being introduced as a tool to draw circles, which causes children to have difficulty in drawing triangles. Last, for the implication of congruence, tessellation suggested in American Everyday Mathematics textbooks is worth being applied to the development of Korean textbooks.

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Modification of Efficient Vitrification Method by Using Open Pulled Straw (OPS) and EM Grid as Vehicles in Human Embryonic Stem Cell (인간 배아 줄기세포의 OPS와 Grid를 이용한 유리화 동결법의 효율성 비교)

  • 박규형;최성준;김희선;오선경;문신용;차광렬;정형민
    • Journal of Embryo Transfer
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    • v.18 no.3
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    • pp.179-186
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    • 2003
  • Human embryonic stem (hES) cell lines have been derived from human blastocysts and are expected to have far-reaching applications in regenerative medicine. The objective of this study is to improve freezing method with less cryo-injuries and best survival rates in hES cells by comparing various vitrification conditions. For the vitrifications, ES cells are exposed to the 4 different cryoprotectants, ethylene glycol (EG), 1,2-propanediol (PROH), EG with dime-thylsulfoxide (DMSO) and EG with PROH. We compared to types of vehicles, such as open pulled straw (OPS) or electron microscopic cooper grids (EM grids). Thawed hES cells were dipped into sequentially holding media with 0.2 M sucrose for 1 min, 0.1 M sucrose for 5 min and holding media for 5 min twice and plated onto a fresh feeder layer. Survival rates of vitrified hES cells were assessed by counting of undifferentiated colonies. It shows high survival rates of hES cells frozen with EG and DMSO (60.8%), or EG and PROH(65.8%) on EM grids better than those of OPS, compared to those frozen with EG alone (2.4%) or PROH alone (0%) alone. The hES cells vitrified with EM grid showed relatively constant colony forming efficiency and survival rates, compared to those of unverified hES cells. The vitrified hES cells retained the normal morphology, alkaline phosphates activity, and the expression of SSEA-3 and 4. Through RT-PCR analysis showed Oct-4 gene expression was down-regulated and embryonic germ layer markers were up-regulated in the vitrified hES cells during spontaneous differentiation. These results show that vitrification method by using EM grid supplemented with EG and PROH in hES cells may be most efficient at present to minimize cyto-toxicity and cellular damage derived by ice crystal formation and furthermore may be employed for clinical application.

GECKO Optical Follow-up Observation of Three Binary Black Hole Merger Events

  • Kim, Joonho;Im, Myungshin;Paek, Gregory S.H.;Lee, Chung-Uk;Kim, Seung-Lee;Chang, Seo-Won;Choi, Changsu;Hwang, Sungyong;Kang, Wonseok;Kim, Sophia;Kim, Taewoo;Lee, Hyung Mok;Lim, Gu;Seo, Jinguk;Sung, Hyun-Il
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.53.3-54
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    • 2021
  • We present optical follow-up observation results of three binary black hole merger (BBH) events, GW190408 181802, GW190412, and GW190503 185404, which were detected by the Advanced Ligo and Virgo gravitational wave (GW) detectors. Electromagnetic (EM) counterparts are generally not expected for BBH merger events, however, some theoretical models suggest that EM counterparts of BBH can possibly arise in special environments. To identify EM counterparts of the three BBH merger events, we observed high-credibility regions of the sky with telescopes of the Gravitational-wave EM Counterpart Korean Observatory (GECKO), including the Korea Microlensing Telescope Network (KMTNet). Our observation started as soon as 100 minutes after the GW event alert and covered roughly 29 - 63 deg2 for each event with a depth of 22.5 mag in R-band within hours of observation. No plausible EM counterparts were found for these events. Our result gives a great promise for the GECKO facilities to find EM counterparts within few hours from GW detection in future GW observation runs.

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Development of Bovine Embryos after Vitrified-Thawed with Electron Microscope Grid and Open Pulled Straws

  • Lee, Y. J.;D.H. Ko;Lee, H. T.;K. S. Chung
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.76-76
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    • 2001
  • The objective of this study was to optimize the vitrification method of in vitro produced bovine embryos. Thus, in vitro produced embryos at 8 cell, morula and blastocyst stages were vitrified on electron microscope grids (EM grids) or in open pulled straws (OPS) with EG5.5 (5.5 M ethylene glycol, 1.0 M sucrose and 10% FBS in m-DPBS medium) freezing solution and their survival rates after thawing were compared. The embryos on EM grids or in OPS were briefly exposed to EG5.5 freezing solution and plunged directly into liquid nitrogen within 30 to 35 sec. Post-thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-DPBS, each for 1 min, and then cultured in CRI aa medium supplemented with 10% FBS. Embryonic survival rate was assessed as re-expanded and hatched rates of those embryos after warming. The rates of re-expansion embryos did not significantly different between EM grid (8 cell: 42.10%, morula: 66.66% and blastocyst: 77.08%) and OPS (8 cell: 47.36%, morula: 61.90% and blastocyst: 83.33%) methods. In addition, the hatched rates in EM grid (8 cell: 31.57%, morula: 57.14% and blastocyst: 72.91%) were similar to those in OPS (8 cell. 34.21%, morula: 50.00% and Blastocyst: 77.08%). Interestingly, even at the same blastocyst stage, the in vitro survival of day 7 embryos (EM grid: 79.48 and OPS: 87.18%) was higher than those of day 8 embryos (EM grid: 72.10 and OPS: 82.06%). The total cell number of blastocyst developed in vitro after vitrification was examined with Hoechst 33342 staining to compare the embryo quality among different treatment groups. The total cell number of blastocyst was not significantly different between vitrified groups (EM grid: 162.4$\pm$8.0 and OPS: 158.4$\pm$7.1) and unvitrified control (168.0$\pm$5.6). These results indicate that both vitrification containers can provide the high rate of embryo survival. Moreover, the OPS container may not need a cap to protect the container from floating after immersion in L$N_2$. Therefore, this study suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification method using EM grid or OPS with EG5.5 freezing solution. In the future, the Pregnancy rate would be investigated after transfer of our vitrified embryos into the appropriated recipients.

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Study on the Differences of Managing to Environmental Issues of Firms between Korea and U.S. (한국과 미국 기업간 환경관리 실천차이에 관한 연구)

  • Jeong, Heon-Bae;Lee, Il-Han
    • International Commerce and Information Review
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    • v.8 no.2
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    • pp.275-296
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    • 2006
  • Environmental management systems offer a set of management tools and principles that can help integrate environmental concerns into daily business practices. Such systems have been in existence since the 1980's. This research identifies the firm's implementation of environmental management that is the most and least popular for managing the environmental management such as 3R's, EM, EMS, environmental awareness. This article investigates the differences of managing to environmental issues of firms between Korea and U.S. The research results suggest that the U.S. firms control of 3R's, EM and EMS better than Korea's, while Korean firms have higher environmental awareness than U.S. firms.

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Analysis of Resonant MTM-TL Using Transmission Line Theory and Its Applications (전송 이론을 이용한 공진 MTM-TL 특성 분석 및 응용)

  • Jang, Seong-Nam;Lee, Bom-Son
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.10
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    • pp.1091-1096
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    • 2009
  • Some closed-form expressions for circuit parameters are derived based on the equivalent circuits for the resonant MTM-TL(open and short). The lumped series resistance and shunt conductance, which explain radiation effects of a unit cell may be found by |$S_{11}$|(simulated or measured) with open and short terminations, respectively. The EM-simulated results, circuit-simulated results(obtained using extracted circuit parameters) and measurement results are shown to be in good agreement.