• Journal of dental hygiene science
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• v.20 no.4
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• pp.213-220
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• 2020

Background: The leaves of Perilla frutescens, commonly called perilla and used for food in Korea, contain components with a variety of biological effects and potential therapeutic applications. The purpose of this study was to identify the components of 70% ethanol extracted Perilla frutescens (EEPF) and determine its inhibitory effects on oral microbial activity and production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharides (LPS)-stimulated Raw264.7 macrophages, consequently, to confirm the possibility of using EEPF as a functional component for improving the oral environment and preventing inflammation. Methods: One kg of P. frutescens leaves was extracted with 70% ethanol and dried at -70℃. EEPF was analyzed using high-performance liquid chromatography analysis, and antimicrobial activity against oral microorganisms was revealed using the disk diffusion test. Cell viability was elucidated using a methylthiazolydiphenyl-tetrazolium bromide assay, and the effect of EEPF on LPS-induced morphological variation was confirmed through microscopic observation. The effect of EEPF on LPS-induced production of pro-inflammatory mediators, NO and PGE2 was confirmed by the NO assay and PGE2 enzyme-linked immunosorbent assay. Results: The main component of EEPF was rosemarinic acid, and EEPF showed weak anti-bacterial and anti-fungal effects against microorganisms living in the oral cavity. EEPF did not show toxicity to Raw264.7 macrophages and had inhibitory effects on the morphological variations and production of pro-inflammatory mediators, NO and PGE2 in LPS-stimulated Raw264.7 macrophages. Conclusion: EEPF can be used as a functional material for improving the oral environment through the control of oral microorganisms and for modulating inflammation by inhibiting the production of inflammatory mediators.

• Journal of Korean Medicine Rehabilitation
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• v.34 no.2
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• pp.15-28
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• 2024

Objectives We used the D-galactose (D-gal) induced C2C12 myoblast senescence model to investigate whether ethanol extract of Perilla. fructescens leaves (EEPF) could delay cellular senescence and regulate related mechanisms. Methods C2C12 myogenic cells were cultured in an incubator under 37 ℃ and 5% CO₂ conditions. EEPF, dried perilla leaves were pulverized and extracted at 1:10 (v/v) at 50 ℃ for 4 hours. Cell counting kit-8 and western blot analysis was performed. Annexin V-FITC apoptosis detection kit and DAPI staining was applied. Catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde analysis kits were used. To measure the level of reactive oxygen species generation, staining and flow cytometry was used. To analyze the mitochondrial activity, membrane potential changes were measured using JC-1. 𝛽-gal activity was analyzed using SA-𝛽-gal staining solution, and DNA damage was analyzed by using 𝛾-H2AX. Quantikine ELISA kit was used to analyze inflammatory cytokine production. Results According to the results of this study, EEPF significantly alleviated the decrease in cell viability in C2C12 cells treated with D-gal and suppressed the decrease in the expression of proliferating cell nuclear antigen. EEPF also markedly blocked D-gal-induced C2C12 cell apoptosis and restored reduced activity of CAT, GSH-Px, T-AOC, SOD. In addition, EEPF suppressed the decrease in 𝛽-galactosidase activity, the induction of DNA damage and the increase in expression of senescence-associated secretory phenotype proteins such as p16, p53 and p21 in D-gal-treated C2C12 cells. Furthermore, EEPF significantly attenuated D-gal-induced production and expression of inflammatory cytokines such as interleukin (IL)-6 and IL-18. Conclusions The results of this study indicate that EEPF can be used as a potential candidate for the prevention and treatment of muscle aging.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.528-528
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• 2012

Recently, atmospheric pressure plasmas attract lots of interests for the useful applications such as surface modification and bio-medical treatment. In this study, a particle-in-cell Monte Carlo collision (PIC-MCC) simulation was adopted to investigate the discharge characteristics of a planar micro dielectric barrier discharge (DBD) with a driving frequency from 13.56 MHz to 162.72 MHz and with a gap distance of 80 micrometers. The variation of frequency, in the change in the electron energy probability function (EEPF). Through the relation between the ion trajectories and the frequency, results in the change of EEPFs is achievable with the turning point of frequency mode. Therefore, it is possible to categorize the efficient operation range of DBDs for its applications by controlling the interactions between plasmas and neutral gas for the generation of preferable radicals.

• Proceedings of the Korean Vacuum Society Conference
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• 2005.08a
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• pp.154-154
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• 2005

• Journal of the Semiconductor & Display Technology
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• v.17 no.1
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• pp.76-83
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• 2018

The electron energy probability function (EEPF) is of significant importance since the plasma chemistry such as the rate of ionization is determined by the electron energy distribution function. It is usually assumed to be Maxwell distribution for 0-D global model. Meanwhile, it has been observed experimentally that the form of EEPF of Ar plasma changes from being two-temperature to Druyvesteyn like as the gas pressure increases. Thus, to apply the 0-D global model of Maxwellian distribution to the non-Maxwellian plasma, we investigated the relative contribution of two distinct electrons with different temperatures. The contributions of cold/hot electrons to the equilibrium state of the plasma have attracted interest and been researched. The contributions to the power and particle balance of cold/hot electrons were studied by comparing the result of the global model considering all combinations of electron temperatures with that of 1-D Particle-in-Cell and Monte Carlo collision (PIC-MCC) simulation and the results of studies were analyzed physically. Furthermore, comparisons term by term for variations of the contribution of cold/hot electrons at different driving currents are presented.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.140.2-140.2
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• 2015

낮은 압력의 평판형 유도 결합 플라즈마 (Inductively Coupled Plasma, ICP)에서 챔버 높이를 바꾸면서 전자 에너지 확률 함수 (Electron Energy Probability Function, EEPF)를 측정하였다. 측정된 전자 에너지 확률 함수에서 기울기가 평평한 부분이 관찰됐고, 이러한 전자 에너지 분포함수의 평평한 부분은 챔버 높이를 증가함에 따라 높은 전자 에너지로 옮겨졌다. 이러한 현상을 분석하기 위해서 2차원 비충돌 가열 메커니즘이 포함된 유도 결합 플라즈마 모델로부터 전자 에너지 확산 계수와 이론적인 전자 에너지 확률 함수를 구하여 실험 결과와 비교하였다. 이를 통하여, 측정된 전자 에너지 확률 함수의 평평한 부분은 전자 튕김 공진 (electron bounce resonance)에 의한 것임을 알 수 있었다.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.251.2-251.2
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• 2014

현재 의료 및 표면처리 분야에 많이 이용되고 있는 상온 대기압 플라즈마 중에서 유전체 격벽 방전(DBD) 장치는 비교적 간단한 구조를 가지며 sub-millimeters 사이즈에서도 매우 높은 플라즈마 밀도의 발생 및 유지가 가능하다. 그러나, 현재로선 이러한 Micro DBD의 특성을 실험적으로 분석하는 것은 장비의 한계가 있으므로, Particle-In-Cell 시뮬레이션을 이용하여 중요 플라즈마 변수들을 관찰하였다. 여기서 사용된 중요변수로는 13.56 MHz~600 MHz사이의 인가 주파수를 두었으며, 유전체 표면에서 양이온에 대한 이차전자 방출계수를 고려하였다, 또한 중성기체는 헬륨가스와 아르곤가스의 2가지 중성기체인 경우를 살펴보았다. 이러한 시뮬레이션을 통해 인가전압의 주기 대비 Ion transit time의 비율이 달라짐에 따라 플라즈마 쉬스의 특성변화와 함께 전자 에너지 확률함수(EEPF)의 특성이 달라진다는 것을 확인하고, 이러한 전자 가열 양상의 변화 원리에 대해서 분석하였다. 또한 주파수 비율 조정을 통한 전자온도, 파워, 효율 등을 조절하는 방법의 공학적 가치에 대해 의견을 제시하였다.

• The Korean Journal of Ecology
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• v.27 no.1
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• pp.1-7
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• 2004

With the emergence and development of ecological engineering in China, and also the research and demonstration of Chinese Ecological Agriculture (CEA), in recent 10 years many types of ecological engineering have been appeared in Southern China, they could be classified into 4 typical patterns. These typical patterns are; 1. Ecological Building (EB), such as ecological public toilet, ecological garden, ecological integrated building, farmers' ecological household, the combine of ecological building and CEA; 2. Ecological Engineering in Paddy Field (EEPF), like rice-fish system, water caltrop-wild ricestem-fish system, mulberry-silkworm-pig-fish system; 3. Ecological Engineering of Agroforestry Systems (EEAF), as tea-food crops intercropping system, rubber-tea-chicken system, bamboo-chicken system, fruit tree-chicken system, metasequoia-fish-duck system; 4. Ecological Engineering Linked with Biogas (EELB), such as animal (pig)-biogas-fish system, animal (pig)-biogas-fruit tree system, animal-biogas-food crops system. Some case studies were presented in the paper. It is showed that these typical patterns have better ecological, social and economic benefits, now they are deeply been extending in southern China.