• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.82-82
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• 1993

허혈-재관류손상 심근세포의 DNA에서 8-hydroxydeoxyguanosine (8-OHdG) 생성을 검토하였다. 흰쥐 적출심장의 Langendorff 관류 표본에서 대동맥 차단에 의한 60분 허혈후 산소가 포화된 Kredb-Henseleit용액으로 30분간 재관류 하므로서 허혈-재관류 손상을 유도하였다. 재관류 후 심근세포에서 DNA를 추출하고 HPLC(EC detector)를 이용하여 8-OHdG를 측정하였다. 실험결과 허혈-재관류 심근세포의 DNA에서 8-OHdG 함량이 증가하였으며 이는 $O_2$ 제거물질인 superoxide dismutase와 OH 제거물질인 mannitol에 의하여 방지되었다. Xanthine oxidase외 경쟁적 길항약인 allopurinol도 8-OHdG 생성을 억제하였으며 단백분해효소 억제제인 phenylsulfonylfluoride 그리고 관류액에서 칼슘의 제거 또한 허혈-재관류 심근 DNA의 생성을 방지하였다. 이상의 결과 허혈심근의 재관류시 8-OHdG 생성이 증가하며 이는 재관류 손상과 같은 산화성 심근손상을 평가하는 좋은 Index가 될 수 있을 것으로 여겨진다.

• Journal of Bio-Environment Control
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• v.12 no.2
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• pp.89-94
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• 2003

This study was conducted to identify the effect of recycling method of drained solution on the concentration of drained solution, and growth and yield of tomato in the recycling system. The recycling methods of drained solution were composed of control, MEC (measurement of EC) and ANS (analysis of nutrient solution). The plant height in the early growth stage was not different among the treatments, but plant fresh weight and dry weight were higher in the MEC or the ANS than in the control. The growth including fruit number, fruit weight, and yield of tomato in the ANS as compared with the control was favorable. The EC of drained solution tended to decrease in the early growth stage, but that of drained solution increased in the late growth stage. It was low in the MEC and the ANS as compared with the control. The pH of drained solution was maintained by 6.2 to 6.5 throughout the growth period in the MEC and the ANS, but the pH of the control increased up to 7.2 at the late growth stage. The N and K concentrations of drained solution tended to decrease in the early growth stage, while those of P, Ca and Mg increased. In the late growth stage, concentrations of N, P, Ca and Mg tended to decrease, but that of K in the ANS was very low. Concentrations of N, P, K, Ca and Mg were lower in the MEC and the ANS than in the control.

• Elastomers and Composites
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• v.48 no.3
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• pp.209-215
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• 2013

On this study, visible-light responsive photocatalyst prepared by sol-gel method was evaluated the effect of the reduction of volatile organic compounds (VOCs) occurred in vehicle interior and its property was examined. According to UV/visible result, visible-light responsive photocatalyst was found that the UV-visible peak is red shift at 420nm, is sensitive in the visible light region. With vehicle interior parts and materials coated visible-light responsive photocatalyst, VOCs was measured by GC/MS. Measuring the VOCs generated from vehicle interior parts and materials, the reduction of VOCs was confirmed.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.5
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• pp.237-245
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• 2017

This study was aimed at investigating effluent water quality and proposing reuse possibilities for 12 sewage treatment plants discharged more than $5,000m^3/day$ in order to recycle the sewage treatment plant effluent of Jeollabuk-do effectively. Additionally, a laboratory scale test for reprocessing water discharge was performed. Categories of reclaimed sewage water reuse were divided into 7 topics and analyzed a total of 28 items including 16 heavy metals based on water quality standard. As a results, color, BOD, TN, chloride and Electrical Conductivity (EC) exceeded reused water quality standard. In particular, color and TN exceeded in 8 and 5 sewage treatment plants, respectively. The value of chloride and EC were high in sewage treatment plants including the food and industrial wastewater. At 4 sewage treatment plants were possible to reuse without re-treatment. The laboratory scale test was conducted to SandFilter (SF)-Granular Activated Carbon (GAC)-MicroFiltraion (MF)-Reverse Osmosis (RO). Both the removal efficiencies and economic feasibility of total E. coli., color and Suspended Solid (SS) suited in case using the SF-GAC treatment method. The removal of chloride and EC had little effect in the case of SF-GAC-MF system, but RO showed over 90% of removal efficiency. After using SF-GAC process only, the concentration of $UV_{254}$ decreased sharply from 0.3651 /cm to 0.0306 /cm and it showed over 92% of removal efficiency rate. In conclusion, for the effective reuse of sewage discharged water, water quality and the surrounding terrain of treatment plants should be all taken into account. If it needed for the reprocessing, both the selection for treatment and economic combination treatment methods will have to be considered.

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.397-402
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• 2009

In Korea, salt stress is one of the major problems limiting crop production and eco-environmental quality in greenhouse soil. The objective of this study was to evaluate the effectiveness of organic residues (Chinese milk vetch, maize stalk, rice straw, and rye straw) for reducing salt activity in greenhouse soil. Organic residues was incorporated with salt-accumulated soil (EC, 3.0 dS $m^{-1}$) at the rate of 5% (wt $wt^{-1}$) and the changes of electrical conductivity (EC) was determined weekly for 8 weeks under incubation condition at $30^{\circ}C$. The EC, microbial biomass carbon (MBC), and water soluble ions in soil was strongly affected by C/N ratio of organic residues. After 8 weeks incubation, the concentration of water soluble $NO_3{^-},\;Ca^{2+}$, and $Mg^{2+}$ was significantly decreased in organic residues having high C/N ratio (maize stalk, rice straw, and rye straw) incorporated soil compared to organic residues having lower C/N ratio (Chinese milk vetch) incorporated soil. The EC value in Chinese milk vetch incorporated soil was higher than control treatment. In contrast, maize stalk, rice straw, and rye straw amended soil was highly decreased the EC value compared to control and Chinese milk vetch applied soil after 4 weeks incubation. Our results indicated that incorporation of organic residues having high C/N ratio (>30) could reduce salt activity resulting from reducing concentration of water soluble ions.

• Proceedings of the Korea Information Processing Society Conference
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• 2000.04a
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• pp.978-983
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• 2000

소비자의 제품 구매에 대한 의사 확인은 전자상거래 시스템(EC System)과 연동한 전자우편 시스템 (E-mail system)에서 발송해 오는 전자우편과, 전자상거래 시스템에 종속적인 관리자도구를 통해 알수 있다. 이러한 정보는 소비자와 판매자간의 시간차를 유발하며, 소비자의 또다른 신속한 정보 획득 요구를 해소 시켜 주지 못한다. 전자우편은 그 자체로 데이터화 될 수 없고 전자상거래를 전략적으로 운영하기 위한 자료로 활용되기 어렵다. 뿐만 아니라 현재의 전자상거래에 있어서 소비자의 대량 구입에 대한 환경은 기존의 일반 상거래와 다른 체계를 갖고 있어서 대량 구매시 판매자와의 직접적인 상담과 할인정보 획득에 대한 소비자와 판매자의 동시적 불만을 유발하고 있다. 이에 따라 본 논문에서는 위와 같은 문제에 대하여 소비자의 구매 의사를 전담하기 위한 에이젼트 시스템을 제안하였고, 전자상거래 시스템에서 발생하던 소비자와 판매자 사이의 시간차와 전략적 운영에 대한 소비자 구매의사 정보의 재이용 문제를 해결하기 위한 것에 대하여 주로 다루고 있다.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.517-524
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• 2018

The purpose of this study was to investigate the density of thrips, and insecticidal resistance for effective control of Western flower thrips in greenhouse. The presence and density of the thrips was investigated using yellow colored-sticky trap in a cucumber field from May to August in Cheon-an. The results of the investigation revealed the existence of the following thrips species; Frankliniella occidentalis, F. intonsa, Thrips palmi, T. tabaci, Scirtothrips dorsalis, Microcephalothrips abdominalis, and T. nigropilosus. The predominant pest was found to be the western flower thrips. To survey the western flower thrips insecticidal resistance, we established the discriminating concentration (DC), recommended concentration (RC) and $2{\times}$recommended concentration ($2{\times}RC$) of nine insecticides; Emamectin benzoate EC, spinetoram SC, Chlorfenapyr EC, Spinosad SC, Cyantraniliprole EC, Acetamiprid WP, Dinotefuran WG, thiacloprid SC and thiamethoxam SC. The bioassay of about five local populations was conducted using the leaf-dipping method. In all local populations, insecticidal resistance in western flower thrips had not developed in emamectin benzoate EC (RC, $10.8{\mu}L\;L^{-1}$), chlorfenapyr EC (RC, $50.0{\mu}L\;L^{-1}$), spinetoram SC (RC, $25.0mg\;L^{-1}$), and spinosad SC (RC, $50.0mg\;L^{-1}$). However, insecticidal resistance in RC was found to have developed in cyantraniliprole EC (RC, $50.0{\mu}L\;L^{-1}$) and four insecticides of neonicotinoid type. Insecticidal activity of 95% or more was observed in each population when cyantraniliprole EC tested in $2{\times}RC$. However, the neonicotinoid types showed different insecticidal activity in $2{\times}RC$.

• Proceedings of the Korean Information Science Society Conference
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• 2001.10a
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• pp.157-159
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• 2001

웹의 표준으로 자리잡고 있는 XML(eXtensible Markup language)은 정보교환을 위한 정보한 포멧으로 대두되고 있으며, 현재 EC/EDI, 전자 도서관, 전자 상거래 등 다양한 분야에서 XML이 사용되고 있다. 따라서 XML 문서를 데이터베이스에 효율적으로 저장하고 검색하기 위한 많은 연구들이 진행되고 있다. XML 문서를 저장하고 검색하고자 하는 시스템은 다양한 시스템의 변화에 효율적으로 적응하고 재사용과 확장성이 용이한 컴포넌트 기반의 소프트웨어로 변환되어야 한다. 본 논문에서는 복잡한 시스템의 개발 기간을 단축하고 소프트웨어 품질과 재사용성을 높이는 객체지향 및 컴포넌트 표준으로 알려진 EJB(Enterprise JavaBeans)를 이용해 XML 문서를 저장하는 방법을 제시 하였다.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.247-252
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• 2011

Electrical conductivity, drainage, and irrigation amount of nutrient solution are important factors for determination of the mixing ratio of fresh and reused nutrient solutions in closed-loop soilless culture. Generally a fixed mixing ratio is applied in commercial scale greenhouses using solar radiation-based irrigation system. Although it ensures continuous supply of fresh nutrient solution in the mixing process, occasional discharge of the drainage is inevitably required. This study was conducted to compare the nutrient replenishing effect under different mixing processes and to investigate appropriate mixing process. For this experiment, a fixed mixing ratio (FR), modifiable mixing ratio (MR), and open-loop (OP) as control were applied. Mixing ratio was determined by a set value of EC for dilution of collected drainage in FR and the set values of 1.0 and $2.0dS{\cdot}m^{-1}$ were used as treatments (FR 1.0 and FR 2.0), respectively. In MR, mixing ratio was determined based on EC and volume of drainage within irrigation volume per event. The volume of drainage stored in the drainage tank tended to increase in FR 1.0. Although such trend was not observed in FR 2.0 and MR, the volume of drainage stored in MR was lower than that in FR 2.0. The ion balance of $Mg^{2+}:K^+:Ca^{2+}$ or $SO^{2-}_4:NO^-_3:PO^{3-}_4$ in the drainage and reused nutrient solution changed within a narrow range regardless of treatment.

• Journal of Life Science
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• v.27 no.8
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• pp.864-872
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• 2017

Equine chorionic gonadotropin (eCG) consists of highly glycosylated ${\alpha}-$ and ${\beta}-subunits$ and is a unique member of the gonadotropin family, because it elicits the response characteristics of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in species other than the horse. To directly assess the biological function of $rec-eCG{\beta}/{\alpha}$, we constructed mammalian expressing vectors of equine luteinizing hormone/chorionic gonadotropin receptors (eLH/CGR). The activity of $rec-eCG{\beta}/{\alpha}$ in vitro assayed in transient transfected CHO-K1 cells and in stably transfected PathHunter Parental cells with eLH/CGR was investigated. $rec-eCG{\beta}/{\alpha}$ was efficiently secreted in the CHO-K1 suspension cell media, and the quantity detected was about 200 mIU/ml from 1 to 7 days after transfection. In the western blot analysis, the $rec-eCG{\beta}/{\alpha}$ protein was broadly identified to be about 40~45 kDa molecular weight. The cAMP stimulation in CHO-K1 cells expressing eLH/CGR was determined to evaluate the activity of $rec-eCG{\beta}/{\alpha}$. The cAMP concentration increased in direct proportion to the concentration of the $rec-eCG{\beta}/{\alpha}$. The $EC_{50}$ value in the transient transfected CHO-K1 cells was $8.1{\pm}6.5ng$. The stable cell lines of eLH/CGR were established in the PathHunter Parental cells expressing ${\beta}-arrestin$. We found that $rec-eCG{\beta}/{\alpha}$ had full LH activity in the PathHunter Parental cells expressing eLH/CGR. The $EC_{50}$ value in transient and stable cells was $5.0{\pm}4.7ng/ml$ and $4.5{\pm}5.2ng/ml$, respectively. These results suggest that $rec-eCG{\beta}/{\alpha}$ has a biological activity in a cell expressing eLH/CGR. These stable cells expressed in PathHunter Parental cells could be useful for elucidating the functional mechanisms of deglycosylated $rec-eCG{\beta}/{\alpha}$ mutants.